首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 46 毫秒
1.
探讨了光照、温度和培养方式对青蒿芽生长和青蒿素合成的影响。适宜芽生长和青蒿素积累的光照强度约为3 000 lx,照光时间为20 h/d ;芽生长和青蒿素积累的最适温度分别为25 ℃和30 ℃,通过先25 ℃(25 d)后30 ℃(5 d) 的温度转变二步培养法可以提高青蒿素的产量;青蒿芽生长和青蒿素积累的最佳培养方式为非浸没低转速摇瓶培养。  相似文献   

2.
适于青蒿芽生长和青蒿素积累的光,温和培养方式探讨   总被引:13,自引:0,他引:13  
探讨了光照,温度和培养方式对青蒿芽生长和青蒿素合成的影响。适宜芽生长和青蒿素积累的光照强度约为3000lx,照光时间为20h/d,芽生长和青蒿素积累的最适温度为25℃和30℃,通过先25℃(25d)后30℃(5d)的温度转变二步培养法可以提高青蒿素的产量;青蒿芽生长和青蒿素积累的最佳培养方式为非浸没代转速摇瓶培养。  相似文献   

3.
利用新型雾化生物反应器培养青蒿不定芽生产青蒿素   总被引:7,自引:0,他引:7  
利用新型的超声雾化内环流生物反应器进行青蒿(ArtemisiaannuaL.)不定芽多层培养生产青蒿素。在新型内环流超声雾化生物反应器中,营养雾沿中心导流筒上升并由其顶端和各开孔处溢出后从环隙落下,2~3min营养雾充满整个反应器。青蒿不定芽在此反应器中生长健壮,形态正常,无玻璃化现象产生,在培养后期,青蒿不定芽长满整个培养空间,部分不定芽可生根。当雾化周期为3min/90min(雾化时间/间隔时间),通气量为0.5L/min时,经25d分批培养,青蒿素产量为46.9mg/L,分别为固体培养和摇瓶培养的2.9和3.2倍。  相似文献   

4.
黄花蒿培养细胞中青蒿素合成代谢的体外调节   总被引:6,自引:0,他引:6  
黄花蒿培养细胞通过两步培养积累青蒿素.第1步在含有0.2~0.4mg/L6-苄基氨基嘌呤(6-BA)和3~4mg/L吲哚乙酸(IAA)的N6培养基中进行细胞的增殖培养,第2步将培养好的细胞转入含0.2~0.4mg/L6-BA和0.2~0.4mg/LIAA的改良N6培养基中进行青蒿素的合成.青蒿素的合成量为190μg/g干细胞左右.当在第2步培养中加入青蒿素合成前体青蒿酸,青蒿素合成量比仅靠激素诱导提高了3倍多.青蒿素的合成途径是植物固醇合成途径的分支途径,当在青蒿素合成过程即第2步培养中加入固醇生物合成抑制剂双氯苯咪唑和氯化氯胆碱处理,可使代谢向合成青蒿素的方向移动,青蒿素合成量明显提高.经200mg/L氯化氯胆碱处理2d,黄花蒿细胞合成青蒿素量为372μg/g干细胞;经20mg/L双氯苯咪唑处理4d,黄花蒿细胞合成青蒿素量为1540μg/g干细胞,比靠激素诱导提高了8倍多,与诱导脱分化细胞的黄花蒿叶中所含的青蒿素(3000μg/g干细胞)处于同一个数量级.以上结果表明:在通过植物激素调节可以合成青蒿素的黄花蒿培养细胞中,缺乏青蒿素合成前体是青蒿素合成量低的重要原因.因此,在青蒿素合成的过程中通过体外调节,  相似文献   

5.
内循环气升式生物反应器培养甘草细胞   总被引:1,自引:0,他引:1  
自行设计研制了7L,9L及25L内循环气升式生物反应器,并应用于甘草细胞的放大培养研究。在接种量为8%(W/V)通气率为0.2—0.25vvm的条件下,甘草细胞在反应器中生长迅速。其中在9L反应器中生长最好,最高生物量达16.25g/L,生长速率达0.9g/L.d,均高于摇瓶培养。培养过程中pH值、溶氧状况通过电极自动显示记录,说明设计的气升式生物反应器适合于甘草细胞的大规模培养。  相似文献   

6.
青蒿素在露水草毛状根中的生物转化   总被引:9,自引:0,他引:9  
露水草毛状根培养系中加入青蒿素培养8d后,青蒿素转化去氧青蒿素。根据光谱数据,对去氧青蒿素的结构进行了鉴定。结果表明,通过水草毛状根能将青蒿素进行选择性还原为去氧青蒿素。  相似文献   

7.
通过正交试验优化筛选了适合黄孢原毛皮革菌降解苯胺的适宜培养基和摇瓶培养降解条件。结果表明:其适宜降解的液体培养基组成为:蔗糖20g/L,可溶性淀粉20g/L,(NH4)2SO4l0g/L,Mn^2 lμmol/L,Tween-800.3%,蛋白胨30g/L。适宜降解的摇瓶培养条件为:接种量为20%、pH为7.0、温度为30℃、培养时间为12d.此条件下的苯胺最高降解率可达95.5%。  相似文献   

8.
液化沙雷氏菌胞外脂肪酶产生条件的研究   总被引:1,自引:0,他引:1  
刘慧  邹文欣  郁文焕   《微生物学通报》1995,22(6):343-346
新分离的一株液化沙雷氏菌(Serratialiquefaciens)能产生大量胞外碱性脂肪酶,本文在摇瓶发酵水平上考察了培养基组成、培养条件等因素对其生成脂肪酶的影响。在优选条件下,即培养基组成为(%)玉米油1.25,豆饼粉2.0,蛋白胨1.0,酵母膏0.2,KHPO0.2,MgSO·7HO0.1,初始pH为7.25时,28℃,150r/min旋转摇床振荡培养40h,该菌株的发酵液酶活达到43u/  相似文献   

9.
在实验室条件下研究方形网纹的生物学。试验时水温25±1℃,饲以斜生栅藻。初生幼体平均体长0.40mm,第1成龄0.96mm,最大体长可达1.45mm,体长增长(1_t,单位mm)与年龄(t,d)的关系可以表达为1_t=1.43-e ̄(-0.268t),平均寿命22.73(17.10-25.00)d,其中包括3个幼龄和13个成龄。在3.91d达到性成熟。7d内生殖3胎,产仔40(19-47)个。一生生殖222.0个幼体,平均每胎18.5(4.8-23.6)个。生殖量最大出现在第6成龄。这时的年龄11.05d,体长1375mm。从初始10个个体的种群计算,种群生长曲线呈Logistic型,可以如下表达式中y表示总个体数,t表示时间(d)。内禀增长能力(r_m)为0.540/个/d,净增殖率(R_o)147.360/世代,世代平均周期(T)9.246d,周限速率(λ)1.716/个/d。  相似文献   

10.
利用气升式内环流生物反应器培养青蒿毛状根生产青蒿素   总被引:8,自引:0,他引:8  
利用自制的气升式内环流生物反应器进行青蒿(ArtemisiaannuaL.)毛状根多层培养生产青蒿素。毛状根培养物在培养过程中均匀分布在生物反应器的筛网间,或以不锈钢网为附着点向四周生长,在25℃和12h/d光照周期下,经20d分批培养获得生物量干重22.57g/L,青蒿素产量374.4mg/L,并对培养过程中蔗糖、磷酸盐、硝酸盐和氨盐消耗的动力学进行了分析。  相似文献   

11.
12.
13.
14.
15.
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.  相似文献   

16.
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.  相似文献   

17.
Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.  相似文献   

18.
正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases  相似文献   

19.
20.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号