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酵母属间原生质体融合改进菌株木糖发酵性能 总被引:2,自引:0,他引:2
通过单倍体分离和紫外诱变,获得了14株树干毕赤酵母(Pichiastipitis)7124和酿酒酵母(Sacharomycescerevisiae)1300的营养缺陷型突变株。用聚乙二醇(PEG)和电诱导融合及致死融合等方法,实现了树干毕赤酵母和酿酒酵母的属间原生质体融合。融合子能发酵木糖产生酒精,其厌氧发酵木糖和木糖葡萄糖混合液的能力明显优于亲株,耐酒精的性能也比亲株树干毕赤酵母7124有所提高。融合子经DNA含量、细胞体积测定和稳定性能实验证明为稳定融合子。 相似文献
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酵母菌属间原生质体融合:构建高产麦角固醇的优良品系 总被引:7,自引:2,他引:5
采用属间原生质体融合技术,对麦角固醇含量较高、细胞生物量低的裂殖酵母(Schziosacch-aromyces)单倍体YG-1-14-58(amet-trp-)与细胞生物量较高、麦角固醇含量低的酿酒酵母(Succh-aromyces)单倍体YG-28-32-135(aade-hix-)进行原生质体融合,在高渗基本选择培养基上挑选融合子,并对获得的大量融合子进行了形态、生理生化、遗传稳定性、细 相似文献
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芽孢杆菌M_(50)产生β甘露聚糖酶的条件研究 总被引:3,自引:0,他引:3
从土壤中分离到9 株产生β甘露聚糖酶的芽孢杆菌( Bacillus sp .) 。Bacillussp . M50250m L三角瓶摇瓶培养试验,以4 % 的魔芋粉为碳源,1-0 % ( NH4)2SO4 为氮源,0-35 %Na2CO3 ,30 ~34 ℃培养60h 产酶达到高峰。酶活力为180 ~200u/m L。100L 罐发酵,在30 ~32 ℃,1∶0 .75vvm 通气量,200r/min 条件下,发酵液酶活力高达330u/m L。酶的最适反应温度和pH 分别为50 ℃和6-0 ,低于50 ℃,pH5 .0 ~7 .0 酶稳定。Fe3+ 、Al3+ 、EDTA、Hg2+ 对酶有抑制作用,而Ba2+ 、Mn2+ 对酶有激活作用。发酵粗酶液对苎麻精干麻精练,显示对精干麻的半纤维素残胶具有降解作用。 相似文献
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以携有结合转座子Tn916的Enterococcus faecalis JH2-2为供体,脱卤脱亚硫酸菌HSS1(Desulfitobacterium dehalogenans,Sm抗性突变株)为受体,在厌氧条件下,通过滤膜杂交、结合转移,将Th916转移并插入到受体菌的染色体上,其转移频率为:1.1×10-7~3×10-8。 在丙酮酸/乳酸-3-氯-4-羟基苯氧乙酸、Tc、Sm培养基上,筛选脱氯呼吸的缺陷型突变株,并用反向PCR(I 相似文献
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在摇瓶培养的基础上,对酵母菌Lipomyces starkeyi HL进行了小型发酵罐的分批和分批补料发酵及其发酵动力学的初步研究。结果表明,通过后期补料既可明显地延长菌体脂类合成期,减缓油脂比合成速率的降低,又可增加菌液的细胞密度,最终提高了整个发酵罐的油脂产量和平均容量产率。发酵结果如下:发酵时间120h;油脂产量11.0g/L;菌体生物量19.4g/L。油脂百分含量 56.5%,显然比分批培养84h所得的11.2g/L细胞生物量和 6.1g/L油脂产量分别增长了73%和80%。此外,通 相似文献
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建立了一个实验室制备重组hGM-CSF的培养、复性和分离纯化的技术工艺。通过对PEGM工程菌的生长和诱导表达等条件的初步调整,使hGM-CSF的表达量从16.9%提高到32.3%,获得0.749g/L的包含体,纯度为65%;包含体用6mol/L盐酸胍溶解和稀释复性后,经CMSepharoseFF.DEAESepharoseFF和SephacrylS200HR层析分离,制得纯度达97%以上、比活性为3.0×107u/mg的hGM-CSF,纯化总回收率约为5% 相似文献
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Michael Hesse 《Plant Systematics and Evolution》1980,134(3-4):229-267
Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout. 相似文献
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Surveillance of Class Ⅰ Newcastle Disease Virus at Live Bird Markets and Commercial Poultry Farms in Eastern China Reveals the Epidemic Characteristics 下载免费PDF全文
Xiaolong Lu Xiaoquan Wang Tiansong Zhan Yifan Sun Xin Wang Naiqing Xu Tianxing Liao Yu Chen Min Gu Shunlin Hu Xiaowen Liu Xiufan Liu 《中国病毒学》2021,36(4):818-822
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正Dear Editor,Parainfluenza virus 5 (PIV5), known as canine parainfluenza virus in the veterinary field, is a negative-sense,nonsegmented, single-stranded RNA virus belonging to the Paramyxoviridae family (Chen 2018). The virus was first reported in primary monkey kidney cells in 1954 (Hsiung1972), then it has been frequently discovered in various 相似文献
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Altaf Hussain Tiantian Wu Hui Li Linjin Fan Kai Li Li Gao Yongqiang Wang Yulong Gao Changjun Liu Hongyu Cui Qing Pan Yanping Zhang Asim Aslam Khan Muti-Ur-Rehman Muhammad Munir Salman Latif Butt Xiaomei Wang Xiaole Qi 《中国病毒学》2019,34(1):102-105
<正>Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However, there is limited genome information of IBDV 相似文献