南极适冷菌Pseudomonassp．C抑菌活性物质的分离纯化与结构鉴定北大核心CSCD

本文对南极适冷菌Pseudomonassp．c发酵液上清中的抗植物病原菌活性物质进行了研究,结果表明在温度40-70℃和pH5—9的范围内发酵液上清的抑菌活性稳定。根据活性追踪,通过硅胶柱层析和高效液相色谱对发酵液上清中的抑菌活性物质进行了分离纯化,得到了3个对常见植物病原真菌尖孢镰刀菌具有抑菌活性的化合物,并对其中的两个化合物进行了结构鉴定。化合物1为环二肽类（环（苯丙氨酸-脯氨酸））,化合物2为壬基酚聚氧乙烯醚类（14-壬基苯氧基．3,6,9,12．四氧十四烷-1-醇）,两者对枯草芽孢杆菌也具有一定的抑菌活性。     

深海放线菌Actinomadura sp.01119的代谢产物研究

采用硅胶柱色谱、制备薄层色谱和凝胶色谱等分离方法,对分离自深海沉积物中的海洋放线菌Actinomadura sp.01119的代谢产物进行研究,从中分离得到9个化合物,经1H NMR、13C NMR和MS等波谱分析并结合相关文献鉴定为环(L-苯丙-L-脯)二肽(1),环(L-脯-L-脯)二肽(2),环(L-酪-L-脯)二肽(3),环(D-酪-L-脯)二肽(4),环(L-异亮-L-脯)二肽(5),环(L-缬-L-脯)二肽(6),棕榈酸(7),对羟基苯甲酸(8),尿嘧啶(9)。其中化合物1~6为环二肽类化合物。     

滑桃树内生真菌Fusarium sp.1RGa-1b中萘醌类代谢产物及其抗菌活性研究

从滑桃树内生真菌Fusarium sp．（1RGa—1b）的发酵代谢产物中分离得到6个一系列的萘醌类色素化合物,通过波谱分析将其结构鉴定为：3-methylether-fusarubin（1）,anhydrofusarubin（2）,2-aeetonyl-3-methyl-5-hydro—gen-7-methoxy—naphthazarin（3）,2-acetonyl-3-methyl-7-methoxy-8-hydrogen—naphthazarin（4）,2-acetonyl-3-methyl-7-methoxy—naphthazarin（5）,2-isopropanol-3-methyl-7-methoxy-naphthazafin（6）。利用纸片扩散法对这些化合物的抗细菌及抗真菌活性进行了初步测试,实验结果表明：化合物3和4具有弱的抗金黄色葡萄球菌的活性。     

△^5—3β，7β-二羟基甾醇及其C-7差向异构体波谱特征及胆碱酯酶的抑制活性

本文对△^5-3β,7β-二羟基甾醇（1—3）和△^5-3β,7α-二羟基甾醇（4～6）的一些核磁共振波谱特征进行了比较。活性测试表明化合物1—6对乙酰胆碱酯酶（AChE）无明显的抑制活性,对丁酰胆碱酯酶（BuChE）则有较强的抑制活性,其中24-亚甲基胆甾-5-烯-3β,7α-二醇（6）的IC50值为9．5μM。通过活性数据比较我们发现7α-羟基甾醇对丁酰胆碱酯酶的抑制活性明显比相应的7β-羟基甾醇高。我们通过计算7位羟基和四环平面之间的二面角角度来尝试解释这些活性差别。     

三株大兴安岭森林凋落物真菌中次生代谢产物及其抗菌活性研究CSCD

本研究采用天然产物分离技术从大兴安岭森林凋落物真菌SGSF622(Berkleasmium sp.)、SGSF289(Oidiodendron sp.)和SGSF062(Parapyrenochaeta sp.)的提取物中获得8个天然产物,经核磁共振、质谱对这些化合物进行了结构鉴定,分别为1-甲氧基-Sch53825(1)、3,4-二羟基-10-甲基-2-亚甲基十六碳-9-烯酸(2)、3,3-二-(3-吲哚)丙烷-1,2-二醇(3)、环(D-色-L-脯)二肽(4)、6-demethylkigelin(5)、lignicol(6)、indole-3-carboxaldehyde(7)和N-(5-amino-2-hydroxy-1-oxopentyl)-tyrosine(8),其中化合物1和化合物2为新化合物。抗菌活性结果显示,化合物2和3具有明显的抗菌活性,其中化合物2活性最强,抗青枯劳尔氏菌(Ralstonia solanacearum)MIC值为7.81μg/mL。     

山竺果壳的化学成分

从山竺（Garcinia mangostana）果壳中分离得到6个化合物,通过MS,1D NMR以及与文献对照鉴定它们为4个[口山]酮类化合物：α-rnangostin（1）,β-mangostin（2）,γ-mangostin（3）,5,9-dihydroxy-8-methoxy-2,2-dimethyl-7-（3-methylbut-2-envl）-2H,6H-pyrano-[3,2-b]-xanthen-6-one（4）,以及表儿茶素（epicatechin,5）和一个双苄类化合物egonol（6）。其中化合物5和化合物6为首次从该植物中分离得到。对化合物1～5进行抗HIV-1 RT活性筛选结果表明,化合物2和化合物5在浓度200μg/ml的条件下,其对HIV-1 RT抑制率分别为41．97％和47．72％;同一实验结果显示化合物1,3和4没有抑制HIV-1 RT作用。     

黄皮根和茎中一个新的木脂体

从黄皮（Clausenalansium）根和茎中分离得到8个化合物,其中化合物claulignan（1）为一个新的木脂体类化合物,通过1D-、2D—NMR和高分辨质谱确定其结构。化合物2-8首次从黄皮属（Clausena）植物中分离得到。活性筛选结果表明,化合物1和6显示一定的细胞毒活性,对人宫颈癌HeLa细胞株的,c50分别为25．03和53．99μM;化合物3具有一定的抗氧化活性,在DPPH实验中的EC50为268．96g·kg-1。     

秦岭地区玉竹根茎的脂溶性成分及其抑菌活性研究

从秦岭产玉竹（Polygonatum odoratum）根茎的石油醚萃取物中分离得到8个化合物,其中有5个化合物为首次从黄精属植物中得到,其结构分别为：（1）（24R/S）-9,19-环阿尔廷-25-烯-3β,24-二醇;（2）α-棕榈酸甘油酯;（3）棕榈酸甲酯;（4）二十八碳酸;（5）（Z）-6-十九碳烯酸。首次对化合物1的抗菌活性进行了测定,发现化合物1在浓度为10μg/mL时,对黄瓜炭疽病原菌的抑制率达到100%;在浓度为100μg/mL时,对灵杆菌的抑菌能力与红霉素相当。     

一株无花果内生真菌及其次生代谢产物

从无花果Ficus carica 叶中分离获得1株具有抗菌活性的内生真菌ZJWCF255,通过形态学和ITS rDNA序列分析将菌种鉴定为炭角菌属Xylaria sp.,其发酵液经活性跟踪分离,采用硅胶柱层析及HPLC等方法获得4个化合物。根据波谱数据,化合物1–4分别鉴定为cytochalasin C、D、Q、R。Cytochalasin Q（3）对11种植物病原真菌均具有较强的抑制活性,对蔓枯病菌的抑制率最强,EC50值为0.04μg/mL。该化合物对3种肿瘤细胞SMMC-772、MCF-7、MGC80-3具有较强体外抑制活性,其IC50值分别为（17.24±2.55）、（7.75±1.37）、（10.30±1.34）μg/mL。首次报道了cytochalasin Q（3）的抗菌活性及对3种肿瘤细胞的体外抑制活性,植物内生真菌是获得抗菌与抗肿瘤活性先导化合物的重要来源。     

Streptomyces sp.KC17012次生代谢产物及抗菌活性研究CSCD

研究Streptomyces sp.KC17012的次生代谢产物。采用硅胶柱色谱、凝胶柱色谱、反相RP-18柱色谱等方法,对Streptomyces sp.KC17012的发酵产物乙酸乙酯萃取部分进行分离纯化,然后采用核磁共振波谱(^(1)H NMR,^(13)C NMR)与质谱方法(MS),结合与文献数据对比,共鉴定18个化合物,分别为seco-((S)-Pro-(R)-Val)(1)、环-(L-脯-L-酪)二肽(2)、环-(D-脯-L-酪)二肽(3)、环-(L-脯-L-苯丙)二肽(4)、色氨酸(5)、amethyldioxindole-3-acetate(6)、3-羧基吲哚(7)、邻氨基苯甲酸(8)、胸腺嘧啶核苷(9)、macrolactin A(10)、红花脂A(11)、(-)-methyl dihydrophaseate(12)、菜豆酸(13)、(-)-ethyl dihydrophaseate(14)、布卢门醇A(15)、3α-hydroxy-5α,6α-epoxy-7-megastigmen-9-one(16)、trogopterin A(17)、和β-谷甾醇(18),其中化合物1~4属于二肽类,11~14为倍半萜类脱落酸类似物。采用微量稀释法检测以上单体化合物的体外抗菌活性,结果表明化合物1和10分别在11和33μmol/L浓度下对枯草杆菌、大肠杆菌、金黄色葡萄球菌具有较好的抑制作用。本研究为进一步挖掘链霉菌KC17012的代谢产物奠定了基础。     

Structure investigation of amphiphilic cyclopeptides in isotropic and anisotropic environments-A model study simulating peptide-membrane interactions.

It has been proposed that the membrane allows a much more efficient binding of certain small or medium-sized amphiphilic messenger molecules to their receptor, not only by accumulation of the drug, but also by induction of orientations and conformations that are much more favorable for receptor docking than structures adopted in isotropic phases. A series of eight amphiphilic cyclic peptides containing lipophilic (L-alpha-aminodecanoic acid = Ada, L-alpha-aminohexadecanoic acid = Ahd, Nhdg = N-hexadecylglycine) and hydrophilic (Lys, Asp) amino acids were synthesized and examined by means of NMR spectroscopy and molecular dynamics (MD) simulations in isotropic (CDCl3) and membrane-mimicking anisotropic (SDS/H2O) solvents to study the influence of the environment on their individual conformations. NMR data of cyclo(-Gly1-D-Asp2-Ahd3-Ahd4-Asp5-Gly6+ ++-) (C4), cyclo(-Lys1-D-Pro2-Lys3-Ada4-Pro5-Ada6-) (C5) and cyclo(-Lys1-Pro2-Lys3-Ada4-D-Pro5-Ada6-) (C6) clearly indicate that those compounds are too rigid to perform a conformational change upon transition from an isotropic to an anisotropic environment. On the other hand, the experimental data of cyclo (-Gly1-Asp2-Ahd3-Ahd4-Asp5-Gly6-) (C1), cyclo(-Asp1-Ala2-Nhdg3-Ala4-D-Asp5-) (C7), and cyclo(-D-Asp1-Ala2-Nhdg3-Ala4-Asp5-) (C8) suggest highly flexible unstructured molecules in both environments. However, for cyclo(-Asp1-Asp2-Gly3-Ahd4-Ahd5-Gly6-) (C2) we observed a structure inducing effect of a membrane-like environment. The compound populates three different conformations in SDS/H2O, whereas in CDCI3 no preferred conformation can be detected. cyclo(-D-Asp1-Asp2-Gly3-Ahd4-Ahd5-Gly6-) (C3) clearly exhibits two different conformations with a shifted beta,beta-turn motif in CDCI3 and SDS/H2O solutions. The conformational change could be reproduced in a restraint-free MD simulation using the biphasic membrane mimetic CCl4/H2O. Our results give clear evidence that membrane interactions may not only lead to structure inductions, but can also induce major conformational changes in compounds already exhibiting a defined structure in isotropic solution.     

New cyclic peptides with osteoblastic proliferative activity from Dianthus superbus

Two new cyclic peptides, dianthins G-H (1 and 2), together with the known dianthin E (3), were isolated from the traditional Chinese medicinal plant Dianthus superbus. The sequences of cyclic peptides 1 and 2 were elucidated as cyclo (-Gly(1)-Pro(2)-Leu(3)-Thr(4)-Leu(5)-Phe(6)-) and cyclo (-Gly(1)-Pro(2)-Val(3)-Thr(4)-Ile(5)-Phe(6)-), on the basis of ESI tandem mass fragmentation analysis, extensive 2D NMR methods and X-ray diffraction. The isolated three compounds all increase proliferation of MC3T3-E1 cells in vitro using MTT method.     

Quorum-sensing cross talk: isolation and chemical characterization of cyclic dipeptides from Pseudomonas aeruginosa and other Gram-negative bacteria

In cell-free Pseudomonas aeruginosa culture supernatants, we identified two compounds capable of activating an N-acylhomoserine lactone (AHL) biosensor. Mass spectrometry and NMR spectroscopy revealed that these compounds were not AHLs but the diketopiperazines (DKPs), cyclo(DeltaAla-L-Val) and cyclo(L-Pro-L-Tyr) respectively. These compounds were also found in cell-free supernatants from Proteus mirabilis, Citrobacter freundii and Enterobacter agglomerans [cyclo(DeltaAla-L-Val) only]. Although both DKPs were absent from Pseudomonas fluorescens and Pseudomonas alcaligenes, we isolated, from both pseudomonads, a third DKP, which was chemically characterized as cyclo(L-Phe-L-Pro). Dose-response curves using a LuxR-based AHL biosensor indicated that cyclo(DeltaAla-L-Val), cyclo(L-Pro-L-Tyr) and cyclo(L-Phe-L-Pro) activate the biosensor in a concentration-dependent manner, albeit at much higher concentrations than the natural activator N-(3-oxohexanoyl)-L-homoserine lactone (3-oxo-C6-HSL). Competition studies showed that cyclo(DeltaAla-L-Val), cyclo(L-Pro-L-Tyr) and cyclo(L-Phe-L-Pro) antagonize the 3-oxo-C6-HSL-mediated induction of bioluminescence, suggesting that these DKPs may compete for the same LuxR-binding site. Similarly, DKPs were found to be capable of activating or antagonizing other LuxR-based quorum-sensing systems, such as the N-butanoylhomoserine lactone-dependent swarming motility of Serratia liquefaciens. Although the physiological role of these DKPs has yet to be established, their activity suggests the existence of cross talk among bacterial signalling systems.     

Staphylopeptide A,a new cyclic tetrapeptide from culture broth of Staphylococcus sp.

Re-investigation of the culture broth of the marine bacterium Staphylococcus sp. (no. P-100826-4-6) afforded a new cyclic tetrapeptide namely staphylopeptide A (1), along with five known compounds, cyclo (l-prolyl-l-valine) (2), cyclo (l-prolyl-l-tyrosine), (3), cyclo (l-prolyl-l-alanine) (4), l-phenylalanine (5), and l-tryptophan (6). The structures of the isolated compounds were determined by extensive IR, 1D (¹H and ¹³C) and 2D (¹H-¹H COSY, HSQC, HMBC, and NOESY) NMR and HRFABMS spectral measurements. The antimicrobial activity of the isolated compounds (1–6) was evaluated.     

Potent cyclic peptide inhibitors of VLA-4 (alpha4beta1 integrin)-mediated cell adhesion. Discovery of compounds like cyclo(MePhe-Leu-Asp-Val-D-Arg-D-Arg) (ZD7349) compatible with depot formulation.

Additional structure-activity relationship studies on potent cyclic peptide inhibitors of very late antigen-4 (VLA-4) are reported. The new N- to C-terminal cyclic hexa-, hepta- and octapeptide inhibitors like cyclo(MeIle/MePhe-Leu-Asp-Val-X) (X = 2-4 amino acids containing hydrophobic and/or basic side chains) were synthesized using solid phase peptide synthesis methods. The peptides were evaluated in in vitro cell adhesion assays and in in vivo inflammation models. Many of the peptides like cyclo(MePhe-Leu-Asp-Val-D-Arg-D-Arg) (ZD7349) (17), cyclo(MeIle-Leu-Asp-Val-D-Arg-D-Arg-D-Phe) (20), cyclo(MeIle-Leu-Asp-Val-D-Arg-D-Arg-MePhe) (21) and cyclo(MePhe-Leu-Asp-Val-D-Arg-D-Arg-D-Ala-D-Ala) (23) were potent inhibitors of VLA-4-mediated cell adhesion and inhibited ovalbumin-induced delayed type hypersensitivity (DTH) response in mice. The more potent compounds were highly selective and did not affect U937 cell adhesion to fibronectin (VLA-5), phorbolmyristate acetate or PMA-differentiated U937 cell adhesion to intercellular cell adhesion molecule-1 (ICAM-1)-expressing Chinese hamster ovary cells (LFA-1) and adenosine diphosphate (ADP)-induced platelet aggregation (GPIIb/IIIa). In contrast to the inhibitors like Ac-cyclo(D-Lys-D-Ile-Leu-Asp-Val) and cyclo(CH2CO-Ile-Leu-Asp-Val-Pip-CH2CO-Ile-Leu-Asp-Val-Pip) described earlier, the new compounds were much more compatible with the depot formulations based on poly(DL-lactide-co-glycolide) polymers. The hexapeptide cyclo(MePhe-Leu-Asp-Val-D-Arg-D-Arg) (ZD7349) (17) inhibited MOLT-4 cell adhesion to fibronectin and vascular cell adhesion molecule-1 (VCAM-1) with IC50 values of 260 and 330 nM, respectively, and did not show any significant effect against other integrins (IC50 > 300 microM). ZD7349 inhibited ovalbumin-induced DTH response in mice when administered continuously using a mini-pump (ED50 0.01 mg/kg/day) or when given as an s.c. or i.v. bolus injection at a dose of 1-10 mg/kg. ZD7349 was also active in type II collagen-induced arthritis (CIA) and experimental autoimmune encephalomyelitis (EAE) tests at a dose of 3-10 mg/kg. The peptide was released from some formulations over a period of 10-20 days. ZD7349 is currently undergoing pre-clinical investigation.     

Lactobacillus plantarum MiLAB 393 produces the antifungal cyclic dipeptides cyclo(L-Phe-L-Pro) and cyclo(L-Phe-trans-4-OH-L-Pro) and 3-phenyllactic acid

We have isolated a Lactobacillus plantarum strain (MiLAB 393) from grass silage that produces broad-spectrum antifungal compounds, active against food- and feed-borne filamentous fungi and yeasts in a dual-culture agar plate assay. Fusarium sporotrichioides and Aspergillus fumigatus were the most sensitive among the molds, and Kluyveromyces marxianus was the most sensitive yeast species. No inhibitory activity could be detected against the mold Penicillium roqueforti or the yeast Zygosaccharomyces bailii. An isolation procedure, employing a microtiter well spore germination bioassay, was devised to isolate active compounds from culture filtrate. Cell-free supernatant was fractionated on a C(18) SPE column, and the 95% aqueous acetonitrile fraction was further separated on a preparative HPLC C(18) column. Fractions active in the bioassay were then fractionated on a porous graphitic carbon column. The structures of the antifungal compounds cyclo(L-Phe-L-Pro), cyclo(L-Phe-trans-4-OH-L-Pro) and 3-phenyllactic acid (L/D isomer ratio, 9:1), were determined by nuclear magnetic resonance spectroscopy, mass spectrometry, and gas chromatography. MIC values against A. fumigatus and P. roqueforti were 20 mg ml(-1) for cyclo(L-Phe-L-Pro) and 7.5 mg ml(-1) for phenyllactic acid. Combinations of the antifungal compounds revealed weak synergistic effects. The production of the antifungal cyclic dipeptides cyclo(L-Phe-L-Pro) and cyclo(L-Phe-trans-4-OH-L-Pro) by lactic acid bacteria is reported here for the first time.     

Synthesis and study of cyclic and linear analogs of neurotensin

New cyclic analogues of neurotensin (NT): [cyclo (13----8), Gly8]NT-(8-13), [cyclo (13----7), Gly7]NT-(7-13), [cyclo (13----5 epsilon), Lys5]NT-(5-13), [cyclo (13----4 epsilon), Lys4]NT-(4-13), and their linear precursors have been synthesized. The latter (protected linear compounds) were prepared by solid-phase peptide synthesis, and cyclization was attained by using diphenylphosphoryl azide. Cyclization of C-terminal hexa- and octapeptide fragments of NT was found to lead to cycloanalogues possessing high depressor activity. As judged by CD spectral data in aqueous solution, the cyclohexapeptide analogue has a relatively rigid conformation different from its linear counter-part and the NT-(9-13) fragment, whereas NT, its cyclohepta- and cyclononapeptides have random structure.     

2,5-Diketopiperazines Produced by Bacillus pumilus During Bacteriolysis of Arthrobacter citreus

We report the detection by gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry analyses of the secreted 2,5-diketopiperazines (DKPs) cyclo(-Ala-Pro), cyclo(-Gly-Pro), cyclo(-Val-Pro), cyclo(-Ile-Pro), cyclo(-Leu-Pro), cyclo(-Pro-Pro), cyclo(-HyP-Pro), cyclo(-Met-Pro), and cyclo(-Phe-Pro) produced by Bacillus pumilus. The study focuses on a marine isolate and a laboratory test strain of B. pumilus with capabilities to lyse pregrown living cell lawns of different bacterial species, among them Arthrobacter citreus. Chromatographic methods were used to analyze induced bioactive compounds. At least 13 different DKPs are produced by B. pumilus. Both strains respond with an increased production of the DKPs cyclo(-Gly-Pro), cyclo(-Ala-Pro), and cyclo(-Val-Pro) to the presence of pasteurized A. citreus cells after 4 h in a nutrient-poor liquid medium. In agar diffusion assays, these DKPs did not cause lysis zones in living cell lawns, but they did inhibit further growth of several pregrown test bacteria in microplates even at concentrations as low as 1 μg ml^?1. Antibiotic substances produced by B. pumilus after 20 h of cultivation in a special lysis medium showed lytic activity in cell-free extracts of B. pumilus culture supernatants.     

Potent cyclic monomeric and dimeric peptide inhibitors of VLA-4 (alpha4beta1 integrin)-mediated cell adhesion based on the Ile-Leu-Asp-Val tetrapeptide.

Potent monomeric and dimeric cyclic peptide very late antigen-4 (VLA-4) inhibitors have been designed based on a tetrapeptide (Ile-Leu-Asp-Val) sequence present in a 25-amino acid peptide (CS-1) reported in the literature. The peptides, synthesized by the SPPS techniques, were evaluated in the in vitro cell adhesion assays and in the in vivo inflammation models. The N- to C-terminal cyclic peptides such as cyclo(Ile-Leu-Asp-Val-NH-(CH2)2-S-(CH2)2-CO) (28) and cyclo(MeIle-Leu-Asp-Val-D-Ala-D-Ala) (31), monomeric and dimeric peptides containing piperazine (Pip) or homopiperazine (hPip) residues as linking groups, e.g. cyclo(MeIle-Leu-Asp-Val-Pip-CH2CO-NH-(CH2)2-S-CH2-CO) (49) and cyclo(MeIle-Leu-Asp-Val hPip-CH2CO-MeIle-Leu-Asp-Val-hPip-CH2CO) (58) and cyclic peptides containing an amide bond between the side chain amino group of an amino acid such as Lys and the C-terminal Val carboxyl group, e.g. Ac-cyclo(D-Lys-D-Ile-Leu-Asp-Val) (62) and beta-Ala-cyclo(D-Lys-D-Leu-Leu-Asp-Val) (68) were more potent than CS-1 in inhibiting the adhesion of the VLA-4-expressing MOLT-4 cells to fibronectin. The more potent compounds were highly selective and did not affect U937 cell adhesion to fibronectin (VLA-5), PMA-differentiated U937 cell adhesion to intercellular cell adhesion molecule- 1-expressing Chinese hamster ovary cells (LFA-1) and ADP-induced platelet aggregation (GPIIb/IIIa). A number of the more potent compounds inhibited ovalbumin-induced delayed type hypersensitivity in mice and some were 100-300 times more potent (ED50 = 0.003-0.009 mg/kg/day, s.c.) than CS-1. Two peptides, Ac-cyclo(D-Lys D-Ile-Leu-Asp-Val) (62) and cyclo(CH2CO-Ile-Leu-Asp-Val-Pip-CH2CO-Ile-Leu-Asp-Val-Pip) (55), were formulated in poly(DL-lactide-co-glycolide) depots and the release profile was investigated in vitro over a 30-day period.     

金铁锁根中的环肽成分

从云南民间重要的药用植物金铁锁（Ｐｓａｍｍｏｓｉｌｅｎｅ ｔｕｎｉｃｏｉｄｅｓ Ｗ．Ｃ．Ｗｕ ｅｔ Ｃ．Ｙ．Ｗｕ）的根中分离得到２个新的天然环二肽以及２个新的环八肽：金铁锁环肽Ａ和Ｂ（ｐｓａｍｍｏｓｉｌｅｎｉｎｓ Ａ ａｎｄ Ｂ）。它们的结构经光谱方法鉴定为ｃｙｃｌｏ（－Ａｌａ－Ａｌａ－）,ｃｙｃｌｏ（－Ｖａｌ－Ａｌａ－）,ｃｙｃｌｏ（－Ｐｒｏ１－Ｐｈｅ１－Ｐｒｏ２－Ｐｈｅ２－Ｐｈｅ３－Ａｌａ－ｐ