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1.
植物样品包埋脱水法超低温保存的研究进展   总被引:15,自引:0,他引:15  
包埋脱水法是植物材料超低温保存的新技术, 从1990 年至今, 已有30 多篇文献报道。本文介绍了包埋脱水法的研究历史、技术要点和主要优点  相似文献   

2.
植物榈包埋脱水法超低温保存的研究进展   总被引:7,自引:0,他引:7  
包埋脱水法是植物材料超低温保存的的新技术,从1990年至今,已有30多篇文献报道。本语文介绍了包埋脱水法的研究历史、技术要点和主要优点。  相似文献   

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文章介绍近10年来有关植物种质包埋脱水超低温保存的研究进展,并对这一方法的应用前景作了展望。  相似文献   

4.
吴雪梅  汤浩茹 《植物学报》2005,22(2):238-245
包埋玻璃化法是在玻璃化法和包埋脱水法基础上发展起来的超低温保存植物种质的新技术。它具有能同时处理大量材料,处理后恢复生长快,对材料的毒害作用较小及成芽率高等优点,已成功地用于辣根、山嵛菜等20余种植物,在植物种质资源的保存上显示出了巨大的应用潜力。本文介绍了包埋玻璃化法产生的背景及其优点, 阐述了包埋玻璃化法的基本方法和预培养、包埋、脱水、化冻及恢复培养等过程,比较了该法冻存后的效果和冻存后所形成植株的遗传稳定性,同时指出了进一步研究的重点。  相似文献   

5.
包埋玻璃化法超低温保存植物种质的研究进展   总被引:29,自引:0,他引:29  
包埋玻璃化法是在玻璃化法和包埋脱水法基础上发展起来的超低温保存植物种质的新技术.它具有能同时处理大量材料,处理后恢复生长快,对材料的毒害作用较小及成芽率高等优点,已成功地用于辣根、山嵛菜等20余种植物,在植物种质资源的保存上显示出了巨大的应用潜力.本文介绍了包埋玻璃化法产生的背景及其优点,阐述了包埋玻璃化法的基本方法和预培养、包埋、脱水、化冻及恢复培养等过程,比较了该法冻存后的效果和冻存后所形成植株的遗传稳定性,同时指出了进一步研究的重点.  相似文献   

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植物种质资源超低温保存现状及其研究进展   总被引:4,自引:0,他引:4       下载免费PDF全文
本文根据联合国粮农组织2010年发布的世界各国的《第二份世界粮食和农业植物遗传资源现状报告以及有关国际会议和相关文献资料,从超低温保存材料类型、基本程序、方法技术、理论基础、影响因素、保存费用、保存策略和保存现状、实际应用等方面综述了全球植物种质资源超低温保存现状及其研究进展,展望了植物种质资源超低温保存技术的应用前景,旨在加强非正常型种子植物种质资源的安全长期保存。文章最后分析了我国存在的差距,提出了今后努力方向和发展的建议。  相似文献   

8.
用包埋脱水法冷冻保存水稻胚性悬浮细胞。整个过程包括:胚性悬浮细胞预培养、细胞包埋、二次预培养、包埋细胞脱水、液氮冰冻,细胞解冻和冷冻细胞恢复培养。结果表明,在细胞水分含量为25.17%和蔗糖浓度依次递增以及第2次预培养34d的存活率最好。在培养基中加2.5g·L^-1活性炭有利于细胞的恢复生长。细胞恢复培养后,能再产生愈伤组织,但生长变慢,有约5d的滞后期。  相似文献   

9.
怀山药种质资源的包埋玻璃化超低温保存与植株再生   总被引:3,自引:0,他引:3  
通过对怀山药(Dioscorea opposita)种质资源的包埋玻璃化超低温保存与植株再生进行研究,结果表明:将低温下锻炼7天的怀山药试管苗带芽茎段放入预培养基中,低温下培养3天,然后在室温下用3%的海藻酸钠和0.5mol·L-1CaCl2包埋,包埋珠用MS+0.2mol·L-1蔗糖+2mol·L-1甘油+50g·L-1二甲基亚砜在0°C下装载60分钟,用30%甘油+15%乙二醇+10%二甲基亚砜+15%聚乙二醇+0.4mol·L-1蔗糖在0°C下脱水60分钟,迅速投入液氮,24小时后立即用40°C水浴快速化冻,再用MS+0.5mol·L-1蔗糖溶液洗涤2次,转入再生培养基中培养,可获得再生植株。再生植株成活率因基因型而异,铁棍山药、太谷山药、怀庆1号山药和B号山药的成活率分别为64.29%、49.21%、13.11%和39.81%。  相似文献   

10.
甘薯种质超低温保存研究进展   总被引:2,自引:2,他引:2       下载免费PDF全文
甘薯是重要的粮食及经济作物,其种质保存一般采用田间种质圃和离体试管苗保存两种方式.但因甘薯的生理特性及这两种保存方式本身存在的弊端,有必要开展新的保存方法研究.超低温保存是目前甘薯种质长期安全保存的最有效方式.本文对甘薯超低温保存的定义、方法及存在的问题和展望进行了论述,并对茎尖玻璃化法进行了详细介绍.  相似文献   

11.
    
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.  相似文献   

12.
Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.  相似文献   

13.
    
正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases  相似文献   

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Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.  相似文献   

17.
    
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.  相似文献   

18.
    
Han  Zhenzhi  Xiao  Jinbo  Song  Yang  Zhu  Shuangli  Wang  Dongyan  Lu  Huanhuan  Ji  Tianjiao  Yan  Dongmei  Xu  Wenbo  Zhang  Yong 《中国病毒学》2021,36(6):1652-1655
  相似文献   

19.
The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.  相似文献   

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Zhao  Li  Gao  Ruqin  Lu  Roujian  Wang  Huijuan  Deng  Yao  Niu  Peihua  Jiang  Fachun  Huang  Baoying  Liang  Jiwei  Jia  Jing  Zhang  Feng  Wang  Wenling  Wu  Guizhen  Tan  Wenjie 《中国病毒学》2021,36(5):1088-1092
  相似文献   

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