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1.
Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation*
《Molecular & cellular proteomics : MCP》2019,18(5):909-922
Highlights
- •Characterization of the phagosomal proteome comparing resting and LPS-treated BMDCs.
- •Label-free quantification determined 2843 phagosomal proteins.
- •Reduced recruitment of hydrolases and V-ATPase to phagosomes of LPS-treated cells.
- •Increased recruitment of antigen cross-presentation molecules to these phagosomes.
2.
《Molecular & cellular proteomics : MCP》2019,18(8):1556-1571
Highlights
- •Functional role of a yet uncharacterized receptor kinase QSK1.
- •Activation model for SIRK1 receptor kinase in a heteromer with QSK1.
- •Role of QSK1 in substrate recruitment and stabilization of the complex.
3.
《Molecular & cellular proteomics : MCP》2019,18(5):818-836
Highlights
- •Kallikrein-related peptidase 7 is over expressed in ovarian cancer.
- •Quantitative PROTOMAP and TAILS approaches identified putative substrates of KLK7.
- •Pro-MMP10 is activated by KLK7.
- •KLK7 cleaves thrombospondin 1 and IGFBP6 in vitro.
4.
《Molecular & cellular proteomics : MCP》2019,18(8):1479-1490
Highlights
- •Frequent genetic polymorphism affects the glycosylation pattern of fetuin.
- •Personalized in-depth proteoform profiling of fetuin purified from 20 donors.
- •Classification of serum donors into three different genotypes.
- •Septic patients show increased level of fucosylation at N-glycolation site N176.
5.
《Molecular & cellular proteomics : MCP》2019,18(6):1171-1182
Highlights
- •First report on the quantitative proteomic profiling of Drosophila lymph glands.
- •Comparative proteomic analysis under conditions of perturbed blood cell homeostasis.
- •Resource for identifying new regulators of insect and vertebrate hematopoiesis.
6.
《Molecular & cellular proteomics : MCP》2019,18(8):1526-1542
Highlights
- •Definition of early systemin-responsive phosphorylation time course.
- •Reconstruction of kinase-substrate relationships from phosphorylation time profiles.
- •Phosphatase PLL5 rapidly dephosphorylated H+-ATPase LHA1 inducing alkalinization of the medium.
- •MAP-Kinase MPK2 re-phosphorylated LHA1 after 15 minutes.
7.
《Molecular & cellular proteomics : MCP》2019,18(6):1085-1095
Highlights
- •Rapamycin and zinc induce moderate but significant mitochondrial proteome changes.
- •The mitochondrial proteins processing system is robust under subtoxic conditions.
- •Rapamycin and zinc perturb the mitochondrial proteins processing system.
- •Rapamycin and zinc perturb the mitochondrial proteins homeostasis.
8.
《Molecular & cellular proteomics : MCP》2019,18(7):1285-1306
Highlights
- •Identification of previously undetected chloroplast envelope proteins.
- •Up to date manual annotation of genuine (or shared) envelope components.
- •New hypotheses for localizations, functions, interactions among cell compartments.
- •A new resource of significant value to the broader plant science community.
9.
《Molecular & cellular proteomics : MCP》2019,18(5):854-864
Highlights
- •Zero-length chemical cross-linking of APOA1 peptides in HDL.
- •Cross-links match antiparallel isomers of APOA dimers in molecular modeling.
- •Identical MS/MS spectra of native and synthetic cross-linked peptides.
- •First biochemical evidence of LL5/5 and LL5/4 isomers in human HDL.
10.
《Molecular & cellular proteomics : MCP》2019,18(10):2089-2098
Highlights
- •Cathepsin-L is introduced as a novel protease for HX-MS studies.
- •Cathepsin-L improves resolution of traditionally challenging histone tails.
- •Cathepsin-L can be readily combined with pepsin for improved protein coverage.
- •In-solution dynamics of the H3.1 and H4 monomers reveal extensive EX1 kinetics.
11.
《Molecular & cellular proteomics : MCP》2019,18(6):1110-1122
Highlights
- •Comprehensive analysis of inter-individual variation of normal urinary proteome.
- •Significant gender differences were observed.
- •Proteins increased in female urine are enriched in immunological pathways.
- •Estimated reference intervals of proteins as the baseline for biomarker discovery.
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14.
《Molecular & cellular proteomics : MCP》2019,18(6):1210-1226
Highlights
- •Quantitative global proteome, acetylome and succinylome of phytoplasma-infected Paulownia tomentosa seedlings.
- •Acetylation may be more important than succinylation in response to phytoplasma infection.
- •Acetylation modified the activities of POR and RuBisCO.
- •Possible model to elucidate the molecular mechanism responses to PaWB from proteome and PTMs.
15.
《Molecular & cellular proteomics : MCP》2019,18(7):1345-1362
Highlights
- •Plant mitoribosomes contain several pentatricopeptide repeat proteins.
- •The small mitoribosomal subunit is of an exceptionally large size.
- •Protein units not directly related to translation may be attached to plant mitoribosomes to confer additional functions to these molecular machines.
16.
《Molecular & cellular proteomics : MCP》2019,18(6):1123-1137
Highlights
- •Changes to the proteome of skin fibroblasts subjected to reductive stress have been quantitated.
- •Only a small set of proteins is selectively diminished upon exposure to reductants.
- •Collagens (COL1A2 and COL6A2) emerge as sentinels of reductive stress.
- •Reductive stress triggers receptor-independent Akt phosphorylation at Ser473.
17.
《Molecular & cellular proteomics : MCP》2019,18(8):1630-1650
Highlights
- •Our search identifies 2,134 kinase-substrate phosphosite pairs in breast cancer.
- •CDKs and MAPKs are dominant regulators of trans substrate-phosphorylation.
- •Druggability, outcomes, and immune signatures related to kinase-substrates.
- •Experimentally validated activated phosphosites of ERBB2, EIF4EBP1, and EGFR.
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19.
《Molecular & cellular proteomics : MCP》2019,18(4):606-621
Highlights
- •In-depth proteome underpins the gland ontogeny and age-specific activity of the HGs.
- •The well-developed acini in the HGs of NBs promote the RJ secretary activities.
- •The enhanced protein and energy metabolism in the HGs boost the stronger RJ secretion of RJBs.
20.
Intact Transition Epitope Mapping – Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE),
《Molecular & cellular proteomics : MCP》2019,18(8):1543-1555
Highlights
- •Multiplex epitope mapping/antigenic determinant identification in the gas phase.
- •Intact transition and controlled dissociation of immune complexes by MS.
- •Simultaneous identification and amino acid sequence determination of epitopes.
- •Simplified in-solution sample handling because of ion manipulation and filtering by MS.