共查询到20条相似文献,搜索用时 31 毫秒
1.
乙型肝炎病毒(HBV)体内外对单个核细胞白细胞介素—1和白细胞介素… 总被引:2,自引:0,他引:2
本研究用PAP法、胸腺细胞增殖法、脾细胞增殖法,分别检测16例体外HBV感染和骨髓单个核细胞与16例慢性乙型肝炎患者体内感染的骨髓单个核细胞中的HBcAg和白细胞介素-1、白细胞介素-2的诱生活性。结果显示,体外HBV感染组成体内HBV感染组骨髓MNCs中HBcAg检出率分别为50%和43.7%。本实验结果表明,HBV在体外感染骨髓MNCs,且与体内自然感染相符,但光镜下未观察到致细胞病变效应。体 相似文献
2.
转甜菜碱醛脱氢酶基因豆瓣菜的耐盐性 总被引:33,自引:0,他引:33
将山菠菜甜苹碱醛脱氢酶(BADH)基因经根癌土壤杆菌(Agrobacterium tumefaciens (Smith et Townsend)Conn)AGL1介导转入豆瓣菜(Nasturtium nofficinale R,Br.)PCR、Southerj bloting检测呈阳性的再生植株有46株,对6株再生植株的BADH活性和Northern bloting检测发现,有5株BADH酶活性明 相似文献
3.
用中国药品生物品检定所检定合格的国内外HBsAgEIA试剂及ABBOTT抗-HBs、抗-HBcEIA试剂,对所收集的13份HBsAg疑难判定血清进行检测,并用PCR方法检测血清中HBVDNA,结果显示国内外HBsAg试剂对部分HBVDNA阳性样品的检出率差异较大,提示这些样品可能为S基因突变株或HBsAg含量较低,因此,HBsAgEIA试剂的敏感度仍有待进一步提高,并应进一步研制检测S基因突变株的 相似文献
4.
5.
乙型肝炎病毒表面抗原aa126 Ile→Ser变异株HBsAg的暂时表达和抗原性鉴定 总被引:1,自引:0,他引:1
曾在一个儿童患者体内,发现一个新的乙型肝炎病毒(HBV)变异株,其HBsAg主蛋白aa126发生Ile(ATT)到Ser(AGT)的取代。已知adr/ayr亚型HBsAg126位为Ile,而adw/ayw亚型HBsAg126位为Thr,表明HBsAg126Ser是一个新的变异株。用计算机做结构分析的结果表明,突变体HBsAg126Ser主蛋白aa120-aa130区段的二级结构与野生型adrHBVHBsAg126Ile相比发生明显的改变。这种构象的变化可能会影响a抗原决定簇(a124-aa147)的抗原性。为了证实这一点,构建了突变S基因表达质粒,在SV40早期启动子的控制下进行抗原表达。利用HBsAg9肽(Thr-Ile126-Pro-Ala-Gln-Gly-Thr-Ser-Met)抗i单克隆抗体和9肽(Thr-Thr126-Pro-Ala-Gln-Gly-Thr-Ser-Met)抗t单克隆抗体进行放射免疫测定,结果表明,这种Ser126突变蛋白对抗i单克隆抗体的反应性比野生蛋白减弱,对抗t单克隆抗体的反应性则与HBsAg126Thr接近。但用三种抗-a单克隆抗体的检测结果揭示,突变蛋白HBsAg126 相似文献
6.
HRP-HBVDNA探针在临检应用中的研究 总被引:2,自引:0,他引:2
本文介绍了一种简便的检测血清HBVDNA的方法。参照Renz等人的标记方法,构建了直接酶标HRP HBVDNA探针。此探针经与固定在硝酸纤维素滤膜上的血清靶DNA杂交后,可通过化学发光自显影检测技术观察结果。敏感度可检测0-1pg靶DNA,相当于同位素探针的灵敏度。对63份HBsAgHBeAg和Anti HBcELISA阳性血清以及24份HBsAgAnti HBc阳性,HbeAg阴性血清用HRP HBVDNA探针进行检测,结果探针HBVDNA阳性率分别为100%(63)和58%(14);对50份HBsAg,ELISA阴性和ALT正常的血清,探针HBVDNA全部阴性。实验结果表明本方法具有很大的推广应用价值。 相似文献
7.
六种固氮蓝藻提取液对玉米的促长作用和提取液成分比较 总被引:4,自引:1,他引:3
本文用六种固氮蓝藻的提取液处理玉米种子,同时对其提取液氨基酸组成和碳水化合物与维生素B12的含量进行了分析。结果表明固氮鱼腥藻HB686(AnabaenaazoticaHB686)、球孢鱼腥藻HB1017(A.sphaericaHB1017)、多变鱼腥藻HB1058(A.variabilisHB1058)和小单歧藻HBTT(TolypothrixtenuisHBTT)的提取液中氨基酸、碳水化合物和维生素B12的含量高于鱼腥藻SP.HB1042(Anabaenasp.HB1042)和繁育管链藻HB38(AulosirafertilissimaHB38)。同时,促进玉米种子萌发和幼苗生长的效果前四种藻较好,后两种则较差。 相似文献
8.
灯盏花总黄酮对血小板聚集和血栓形成的影响 总被引:20,自引:2,他引:18
采用Bom氏比浊法测定灯盏花总黄酮(Erigeron breviscapus flavones,EBF)在体内和体外对血小板活化聚集的影响。应用Kohler等法观察EBF对小鼠尾静脉注射花生四烯酸(AA)所致突然死亡的保护作用。结果表明,EBF在体外和体内对ADP、AA或血小板活化因子(PAF)引起的血小板聚集均有明显抑制作用,体外实验,其效应呈浓度依赖关系,体内试验,于给药后180min达最大抑 相似文献
9.
用套式多聚酶链反应(Nested-PCR)技术对169对HBsAg及HBsAg/HBeAg阳性孕妇及其新生儿外周血清进行了HBV-DNA检测,103对HBsAg阳性孕妇及其新生儿外周务中HBV-DNA阳性率分别为72.8%和33.0%;66对HBsAg和HBeAg双阳性的孕妇及其新生儿外周血清中HBV-DNA阳性率分别为86.4%和43.9%,对55例HBsAg及HBsAg/HBeAg阳性产妇产后 相似文献
10.
促进紫草色素合成的米曲霉诱导子的纯化和活性鉴定 总被引:1,自引:0,他引:1
米曲霉(Aspergillusoryzae)诱导子来源于该真菌的菌丝体部分。诱导子粗提物通过DEAE-Cellu-lose、Sepharose-4B、Bio-Gelp-4、732 柱层析,得到既不吸附于阴离子交换纤维素又不吸附于阳离子交换树脂、分子量约为1200~2200 D、糖含量为粗提物6.7% 的组分FⅠb2-H,与粗提物相比它的诱导子相对活性提高了120 倍。诱导子粗提物中还含有低浓度不影响滇紫草(Onosm a paniculatum )细胞合成紫草色素、而高浓度抑制紫草色素合成的核酸类组分FⅡ,核苷酸、氨基酸类组分FⅠb2-Na 及强烈抑制紫草色素合成的多糖类组分FⅠa。FⅠa可诱导细胞合成对照中没有的一种黄色素,是另一类代谢产物的诱导子 相似文献
11.
12.
Profiles of SARS-CoV-2 RNA and Antibodies in Inpatients with COVID-19 not Related with Clinical Manifestation: A Single Centre Study 下载免费PDF全文
Zhao Li Gao Ruqin Lu Roujian Wang Huijuan Deng Yao Niu Peihua Jiang Fachun Huang Baoying Liang Jiwei Jia Jing Zhang Feng Wang Wenling Wu Guizhen Tan Wenjie 《中国病毒学》2021,36(5):1088-1092
13.
14.
15.
RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
16.
JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献
17.
Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
18.
正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献
19.