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1.
小麦吸收土壤磷转运子在酵母突变体中的功能互补分析   总被引:5,自引:1,他引:4  
以小麦磷转运子全长编码cDMA(TaPT2)为探针与小麦基因组DNA进行Southern杂交,结果表明,在小麦基因组中存在该基因的不同家族成员,另外,将TaPT2基因转入酵母突变体MB192中,以野生型菌株YPH084为对照,分别检测YTaPT2,YPH084和MB192酸性磷酸酶分泌情况,生长情况以及对培养基的磷吸收情况,得到结论:TaPT2的功能与酵母磷转运子编码基因PHO84相似,具有增强酵母吸收磷的作用。  相似文献   

2.
磷、硫转运蛋白是大豆(Glycine max(L.)Merr.)体内磷、硫转运的重要载体,参与调节磷和硫酸盐的吸收与转运,对提高大豆的磷、硫利用效率至关重要。大豆磷转运蛋白可划分为Pht1、Pht2、Pht3、Pho1和Pho2 5大家族,目前对Pht1的研究最为深入。大豆14个Pht1家族可分为3个亚家族,他们对磷吸收和转运具有重要作用。大豆硫转运蛋白基因GmSULTR1;2b可在大豆根中特异性表达并被低硫胁迫诱导。本文基于大豆磷、硫的营养吸收、转运与利用过程中的相关性,对Pht1家族以及GmSULTR1;2b基因在大豆中的研究进展进行了综述,并对近年来大豆磷、硫转运蛋白的研究进展及未来的研究方向进行了展望。  相似文献   

3.
Pht3(phosphate transporter 3)磷转运子家族属于一类低亲和力磷转运蛋白,在调节植株体内磷素的动态平衡中发挥重要作用。为了初步探讨玉米中Zm Pht3;1基因的结构特征及其磷饥饿的响应机制,利用同源克隆的方法从耐低磷玉米自交系Mo17中分离得到Zm Pht3;1基因,并运用实时荧光定量PCR和亚细胞定位的方法对其进行深入研究。结果表明,Zm Pht3;1的编码区全长1 101 bp,编码366个氨基酸,含有典型的线粒体转运家族(mitochondrial carrier family,MCF)结构特征与6个疏水跨膜结构。荧光定量PCR分析表明,该基因在两个极端材料的根系与叶片中均有表达,而表达模式差异显著,在耐低磷玉米自交系Mo17的根系和叶片中表现为缺磷胁迫前期的一般性反应和后期的特异性反应。转化烟草的亚细胞定位结果显示,Zm Pht3;1主要分布于细胞膜上,可能是一个双亲和转运体,在玉米响应磷饥饿胁迫过程中发挥重要的适应性调节作用。  相似文献   

4.
Pht1家族磷酸盐(Pi)转运体介导植物中磷(P)的吸收和再动员。为探讨甘草Pht1基因的结构及表达模式,该研究利用生物信息学方法对甘草Pht1(GuPht1)基因家族进行分析,结合转录组数据和实时荧光定量(qRT-PCR)分析GuPht1在非生物胁迫下的表达,并采用RT-PCR克隆4个GuPht1基因。结果显示:(1)甘草中有8个Pht1家族成员(GuPht1;1—GuPht1;8),都位于细胞膜上,且具有12个跨膜结构,属于MFS超家族,氨基酸长度介于521~570 aa之间,含有Pht1保守的特征序列GGDYPLSATIMSE。(2)系统进化分析显示,甘草GuPht1基因家族成员与豆科植物亲缘关系较近;启动子区含有与磷饥饿有关的W-box、G-box、PHO-like和P1BS元件;甘草GuPht1基因家族在Scaffold定位分布均匀,三级结构均为单体。(3)转录组数据分析显示,GuPht1响应干旱、盐、激素等胁迫,且表达有组织特异性。qRT-PCR结果表明,低磷胁迫下GuPht1基因有明显的时空表达差异性,GuPht1;1/1;6/1;8在根中表达明显上调,GuPht1;5/...  相似文献   

5.
以油茶‘湘林4号’为材料,通过 RT-PCR 和 RACE 的方法克隆出油茶磷酸转运子Pht1基因家族一个成员的全长cDNA序列,命名为CoPht1;1(GenBank登录号:JX403969),通过实时定量 PCR 的方法检测了不同磷浓度下该基因在根系中的表达水平。结果表明:CoPht1;1 CDS长度为 1 626 bp,编码 542 个氨基酸,与其他物种的Pht1氨基酸序列具有较高的相似性,其中与夹竹桃科长春花的 Pht1相似性最高,达到 88%;蛋白质二级结构和拓扑结构预测表明,CoPht1;1具有跨膜蛋白的主要特征,与其他物种的Pht1具有一致性;实时定量 RT-PCR 结果表明,油茶Pht1基因的表达受低磷因素诱导,并随磷处理时间的不同而变化。  相似文献   

6.
高亲和磷转运蛋白负责植物在低磷条件下吸收和转运磷酸盐,对植物的生长发育至关重要。将水稻中关键的高亲和磷转运蛋白基因OsPT8(A high affinity phosphate transporter gene OsPht1;8,以下简称OsPT8)通过农杆菌介导的方法转入烟草云烟87,以转基因烟草和野生型(云烟87)为材料,设置正常供磷(1 mmol/L Pi)和低磷(0.1 mmol/L Pi)两个处理的沙培试验,检测烟株地上部和地下部的生物量、全磷及有效磷的含量,分析烟草高亲和磷转运蛋白家族基因(NtPT1和NtPT2)的表达差异。结果显示,低磷条件下,OsPT8过量表达转基因株系生物量均显著高于野生型;在正常供磷和低磷条件下,OsPT8过量表达烟草株系全磷含量和有效磷含量均显著高于野生型,这表明高亲和磷转运蛋白基因OsPT8可以提高转基因烟草的耐低磷能力。RT-PCR和Q-PCR结果显示,转基因株系显著提高了烟草高亲和磷转运蛋白基因NtPT1和NtPT2的表达量,表明OsPT8对烟草磷吸收和转运的影响是通过OsPT8基因和烟草NtPT1、NtPT2基因等一个复杂的过程起作用的。  相似文献   

7.
低磷胁迫水稻根部基因表达谱研究   总被引:3,自引:0,他引:3       下载免费PDF全文
磷是植物体内重要的营养元素.土壤中含磷总量丰富,但能被植物直接吸收利用的可溶性磷含量却很低,这成为制约农作物产量的重要因素.本研究利用水稻寡核苷酸芯片分析了水稻根部在正常营养条件和低磷胁迫6,24,72h3个时间点的全基因组表达谱.和正常营养条件下相比,低磷胁迫水稻根部共发现795个差异表达基因.差异表达基因功能分析发现:(1)磷酸盐转运蛋白、酸性磷酸酶、RNA酶等基因上升表达;(2)糖酵解等与能量代谢相关基因先上升后下降表达;(3)氮吸收和脂代谢相关基因改变其表达;(4)蛋白质降解、细胞衰老相关基因上升表达;(5)部分跨膜转运蛋白基因表达上调.研究结果为进一步揭示植物低磷胁迫反应机制,改善作物对磷吸收利用效率提供了有用的信息.  相似文献   

8.
利用抑制性扣除杂交技术克隆水稻磷饥饿诱导基因   总被引:3,自引:0,他引:3  
磷素是植物生长所必需的重要元素。在缺磷环境中,植物能够调节自身的形态、生理生化和基因表达水平来适应环境的变化。为研究水稻(Oryzn sativa L.)耐低磷胁迫的分子机理,采用抑制性扣除杂交技术(SSH)构建磷饥饿诱导的水稻根系扣除cDNA文库。通过文库筛选和测序获得18个已知基因和47个功能未知基因。这些基因参与了不同的代谢过程,包括磷吸收和转运、信号传导、蛋白质合成和降解、碳水化合物代谢和胁迫反应。Northern杂交结果表明,在磷饥饿胁迫下这些基因呈现不同的表达模式,并且不同代谢过程中的基因对磷饥饿有着不同的反应。  相似文献   

9.
植物菌根共生磷酸盐转运蛋白   总被引:1,自引:0,他引:1  
大多数植物能和丛枝菌根(arbuscular mycorrhiza, AM)真菌形成菌根共生体。AM能够促进植物对土壤中矿质营养的吸收,尤其是磷的吸收。磷的吸收和转运由磷酸盐转运蛋白介导。总结了植物AM磷酸盐转运蛋白及其结构特征,分析其分类及系统进化,并综述了AM磷酸盐转运蛋白介导的磷的吸收和转运过程及其基因的表达调控。植物AM磷酸盐转运蛋白属于Pht1家族成员,它不仅对磷的吸收和转运是必需的,而且对AM共生也至关重要,为进一步了解菌根形成的分子机理及信号转导途径提供了理论基础。  相似文献   

10.
水稻磷酸盐转运蛋白基因的克隆、表达及功能分析   总被引:5,自引:0,他引:5  
以水稻叶片为材料, 设计一对特异引物, 获得了编码磷酸盐转运蛋白基因OsPT6:1. 聚类和氨基酸保守位点分析指出该基因可能为水稻高亲和力磷酸盐转运蛋白编码基因. 原位杂交与RT-PCR表达结果确定此基因在根与叶片中均表达, 尤以低磷诱导下叶片的叶肉细胞表达量最高. 同源重组表明该基因的表达可以提高毕氏酵母对磷素的吸收效率, 同时其基因的导入可以使高亲和力磷酸盐转运蛋白缺失的酵母突变体的磷素吸收功能得以恢复. 以上结果表明, OsPT6:1为水稻高亲和力磷酸盐转运蛋白的编码基因.  相似文献   

11.
Several phosphate transporters (PTs) that belong to the Pht2 family have been released in bioinformatics databases, but only a few members of this family have been functionally characterized. In this study, we found that wheat TaPHT2;1 shared high identity with a subset of Pht2 in diverse plants. Expression analysis revealed that TaPHT2;1 was strongly expressed in the leaves, was up-regulated by low Pi stress, and exhibited a circadian rhythmic expression pattern. TaPHT2;1–green fluorescent protein fusions in the leaves of tobacco and wheat were specifically detected in the chloroplast envelop. TaPHT2;1 complemented the Pi transporter activities in a yeast mutant with a defect in Pi uptake. Knockdown expression of TaPHT2;1 significantly reduced Pi concentration in the chloroplast under sufficient (2 mM Pi) and deficient Pi (100 μM Pi) conditions, suggesting that TaPHT2;1 is crucial in the mediation of Pi translocation from the cytosol to the chloroplast. The down-regulated expression of TaPHT2;1 resulted in reduced photosynthetic capacities, total P contents, and accumulated P amounts in plants under sufficient and deficient Pi conditions, eventually leading to worse plant growth phenotypes. The TaPHT2;1 knockdown plants exhibited pronounced decrease in accumulated phosphorus in sufficient and deficient Pi conditions, suggesting that TaPHT2;1 is an important factor to associate with a distinct P signaling that up-regulates other PT members to control Pi acquisition and translocation within plants. Therefore, TaPHT2;1 is a key member of the Pht2 family involved in Pi translocation, and that it can function in the improvement of phosphorus usage efficiency in wheat.  相似文献   

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13.
Chen A  Hu J  Sun S  Xu G 《The New phytologist》2007,173(4):817-831
Here, orthologous genes of six phosphate transporter (PiT) genes, which are members of the Pht1 and Pht2 families in tomato and potato, have been cloned from the solanaceous species pepper, eggplant and tobacco. Overall, expressions of these genes in pepper, eggplant and tobacco showed similar patterns to those in tomato and potato: P-starvation enhancement in both leaves and roots for Pht1;1, P-depletion induction exclusively in roots for Pht1;2, mycorrhizal enhancement for Pht1;3, and mycorrhizal induction for both Pht1;4 and Pht1;5. In the roots of nonmycorrhizal eggplant, SmPht1;3, SmPht1;4 and SmPht1;5 were also expressed under extreme P starvation. Mycorrhizal symbiosis under high-P supply conditions reduced plant growth, with concurrent enhancement of Pht1;2 expression in the roots of pepper as well as eggplant. In addition, the mycorrhizal symbiosis down-regulated the expression of Pht2;1 genes greatly in the leaves of pepper and tobacco. The discrepancies between the evolutionary distances of the PiT genes and their expression patterns among the five species suggest greater complexity in function of PiT in plants than previously expected.  相似文献   

14.
Phosphate transport in plants   总被引:19,自引:5,他引:14  
Smith  Frank W.  Mudge  Stephen R.  Rae  Anne L.  Glassop  Donna 《Plant and Soil》2003,248(1-2):71-83
Transport of inorganic phosphate (Pi) through plant membranes is mediated by a number of families of transporter proteins. Studies on the topology, function, regulation and sites of expression of the genes that encode the members of these transporter families are enabling roles to be ascribed to each of them. The Pht1 family, of which there are nine members in the Arabidopsis genome, includes proteins involved in the uptake of Pi from the soil solution and the redistribution of Pi within the plant. Members of this family are H2PO4 /H+ symporters. Most of the genes of the Pht1 family that are expressed in roots are up-regulated in P-stressed plants. Two members of the Pht1 family have been isolated from the cluster roots of white lupin. These same genes are expressed in non-cluster roots. The evidence available to date suggests that there are no major differences between the types of transport systems that cluster roots and non-cluster roots use to acquire Pi. Differences in uptake rates between cluster and non-cluster roots can be ascribed to more high-affinity Pi transporters in the plasma membranes of cluster roots, rather than any difference in the characteristics of the transporters. The efficient acquisition of Pi by cluster roots arises primarily from their capacity to increase the availability of soil Pi immediately adjacent to the rootlets by excretion of carboxylates, protons and phosphatases within the cluster. This paper reviews Pi transport processes, concentrating on those mediated by the Pht1 family of transporters, and attempts to relate those processes involved in Pi acquisition to likely Pi transport processes in cluster roots.  相似文献   

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16.
TaPHT1.2 is a functional, root predominantly expressed and low phosphate (Pi) inducible high-affinity Pi transporter in wheat, which is more abundant in the roots of P-efficient wheat genotypes (e.g., Xiaoyan 54) than in P-inefficient genotypes (e.g., Jing 411) under both Pi-deficient and Pi-sufficient conditions. To characterize TaPHT1.2 further, we genetically mapped a TaPHT1.2 transporter, TaPHT1.2-D1, on the long arm of chromosome 4D using a recombinant inbred line population derived from Xiaoyan 54 and Jing 411, and isolated a 1,302 bp fragment of the TaPHT1.2-D1 promoter (PrTaPHT1.2-D1) from Xiaoyan 54. TaPHT1.2-D1 shows collinearity with OsPHT1.2 that has previously been reported to mediate the translocation of Pi from roots to shoots. PrTaPHT1.2-D contains a number of Pi-starvation responsive elements, including P1BS, WRKY-binding W-box, and helix-loop-helix-binding elements. PrTaPHT1.2-D1 was then used to drive expression of 13-glucuronidase (GUS) reporter gene in Arabidopsis through Agrobacterium-mediated transformation. Histochemical analysis of transgenic Arabidopsis plants showed that the reporter gene was specifically induced by Pi-starvation and predominantly expressed in the roots. As there is only one SNP between the TaPHT1.2-D1 promoters of Xiaoyan 54 and Jing 411, and this SNP does not exist within the Pi-starvation responsive elements, the differential expression of TaPHT1.2 in Xiaoyan 54 and Jing 411 may not be caused by this SNP.  相似文献   

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Background

Phosphorus (P) is essential for plant growth and development. Phosphate (Pi) transporter genes in the Pht1 family play important roles in Pi uptake and translocation in plants. Although Pht1 family genes have been well studied in model plants, little is known about their functions in soybean, an important legume crop worldwide.

Principal Findings

We identified and isolated a complete set of 14 Pi transporter genes (GmPT1-14) in the soybean genome and categorized them into two subfamilies based on phylogenetic analysis. Then, an experiment to elucidate Pi transport activity of the GmPTs was carried out using a yeast mutant defective in high-affinity Pi transport. Results showed that 12 of the 14 GmPTs were able to complement Pi uptake of the yeast mutant with Km values ranging from 25.7 to 116.3 µM, demonstrating that most of the GmPTs are high-affinity Pi transporters. Further results from qRT-PCR showed that the expressions of the 14 GmPTs differed not only in response to P availability in different tissues, but also to other nutrient stresses, including N, K and Fe deficiency, suggesting that besides functioning in Pi uptake and translocation, GmPTs might be involved in synergistic regulation of mineral nutrient homeostasis in soybean.

Conclusions

The comprehensive analysis of Pi transporter function in yeast and expression responses to nutrition starvation of Pht1 family genes in soybean revealed their involvement in other nutrient homeostasis besides P, which could help to better understand the regulation network among ion homeostasis in plants.  相似文献   

19.
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