凹叶瑞香的化学成分研究(英文)

从凹叶瑞香的乙酸乙酯部位分离得到14个化合物,经理化性质及波谱数据分析分别鉴定为瑞香黄烷A(1),瑞香黄烷B(2),瑞香黄烷C(3),瑞香黄烷E(4),瑞香黄烷F(5),芫花素(6),芫根苷(7),4,′5-二羟基-3,′7-二甲氧基黄酮(8),瑞香新素(9),5′′-去甲氧基瑞香新素(10),左旋松脂酚(11),瑞香醇酮(12),紫丁香苷(13)和丁香醛(14)。化合物1～14均为首次从该植物中分离得到。     

金边瑞香有机相提取物库的构建及活性成分分离纯化研究

采用逆流提取-系统溶剂组分化-高效液相色谱法(high-performance liquid chromatography,HPLC)检测组分化效果-高速逆流色谱技术(High-Speed Counter-Current Chromatography,HSCCC)分离制备高纯度化合物的研究思路,对金边瑞香化学成分进行研究得到4个高纯度单体化合物.通过现代谱学方法分别鉴定为:瑞香素(daphnetin,1)、瑞香黄烷I(daphnodorin I,2)、对羟基苯甲酸(p-hydroxybenzonic acid,3)和瑞香黄烷D1(daphnodorin D1,4),化合物2～4均为首次从该种植物中分离得到.     

瑞香狼毒化学成分研究

从瑞香狼毒95%乙醇提取物中分离得到了17个化合物,根据理化性质和波谱数据鉴定为4’-甲氧基-7-羟基黄酮(1),山奈酚(2),5,4’-二羟基-7-甲氧基二氢黄酮(3),4’,5,7-三羟基黄烷酮(4),3,5,7-三羟基黄烷酮(5),山奈酚-7-O-β-D-葡萄糖苷(6),α-香树酯醇(7),(24R)-5α-豆甾-7,22(E)-二烯-3α-醇(8),狼毒色原酮(9),伞形花内酯(10),东莨菪素(11),异东莨菪素(12),狼毒素(13),瑞香内酯(14),异茴芹香豆素(15),β-谷甾醇(16),瑞香素乙(17)和β-胡萝卜甙(18)。其中化合物1-8为首次从该植物中分离得到。     

黄芪注射液的化学成分

采用正、反相硅胶柱层析从黄芪注射液原液中分离纯化出 14个化合物 ,经波谱分析鉴定了它们的结构。其中 6个为异黄酮化合物 ,分别是芒柄花素 (1) ,毛蕊异黄酮 (2 ) ,6″ O 乙酰基芒柄花苷 (3) ,芒柄花苷 (7) ,红车轴草异黄酮 7 O β D 吡喃葡萄糖 (12 ) ,毛蕊异黄酮 7 O β D 吡喃葡萄糖 (13) ;1个紫檀烷化合物 ,结构为 9,10 二甲氧基紫檀烷 3 O β D吡喃葡萄糖 (4) ;1个异黄烷化合物 ,结构为 2′ 羟基 3′ ,4′ 二甲氧基异黄烷 7 O β D 吡喃葡萄糖 (6 ) ;另外 6个为黄芪皂苷类化合物 ,分别是乙酰黄芪皂苷Ⅰ(5 ) ,黄芪皂苷Ⅰ (8) ,异黄芪皂苷Ⅰ (9) ,异黄芪皂苷Ⅱ (10 ) ,黄芪皂苷Ⅱ (11)和黄芪甲苷 (14)。其中化合物 3系首次从黄芪属植物中分离得到。     

文殊兰叶子化学成分及抗炎活性研究

本文对西双版纳傣药痹通剂的配方植物文殊兰的叶子进行了化学成分分离以及抗炎活性研究。通过色谱柱分离纯化,从文殊兰叶子的甲醇提取物中分离得到13个化合物,并运用现代波谱学技术分别鉴定为:7,4'-二羟基黄烷(1)、7,4'-二羟基-8-甲基黄烷(2)、杜鹃素(3)、stigmast-4-ene-3β,6β-diol(4)、byzantionoside B(5)、daucosterol+stigmasterol-3-O-glucopyranoside(6)、(6R,9R)-3-oxo-α-ionol-9-O-β-D-glucopyranoside(7)、5-hydroxy-6,7-methylenedioxy-2-methylchromone(8)、正丁基吡喃果糖苷(9)、豆甾醇(10)、豆甾醇苷(11)、β-谷甾醇(12)、胡萝卜苷(13),其中化合物2~8为首次从该植物中分离得到。文殊兰叶子提取物具有显著的NO抑制活性,即显著的抗炎活性,本次实验分离得到的化合物7,4'-二羟基-8-甲基黄烷(2)和杜鹃素(3)具有一定的抗炎活性。     

秀雅杜鹃化学成分的研究

从秀雅杜鹃全草的石油醚部分和乙酸乙酯部分中分离出20个化合物,利用现代波谱技术(MS1、HNMR、13CNMR、DEPT、CD)和化学方法对其中17个化合物进行了结构鉴定,分别为2S-4′,5,7-三羟基黄烷酮(1)、(2R,3R)-( )花旗松素(2)、扁蓄苷(3)、槲皮素-3-O--αL-鼠李糖苷(4)、金丝桃苷(5)、槲皮素(6)、16,17-二羟基,11β-贝壳杉烷-19酸(7)、异莨菪亭(8)、喇叭茶醇(9)、豆甾-4-烯-6β-醇-3-酮(10)、β-香树脂醇(11)、α-香树脂醇(12)、28-羟基-β-香树脂醇(13)、山楂醇(14)、齐墩果酸(15)、β-谷甾醇(16)、β-胡萝卜苷(17).所有化合物均为首次从该植物中分得,其中化合物1、7、8、9、10为首次从该属植物中发现.     

大苞鞘石斛化学成分研究

通过硅胶柱层析、纯化,从大苞鞘石斛中分离得到5个化合物,利用理化性质和波谱分析分别鉴定为钩状石斛素(1)、愉悦石斛素(2)、4',5,7-三羟基-3′,5′-二甲氧基黄烷酮(3)、β-胡萝卜苷(4)和β-谷甾醇(5).所有化合物均为首次从大苞鞘石斛中分离得到.     

钩枝藤化学成分研究

采用95%乙醇提取,石油醚、乙酸乙酯萃取分部,利用反复硅胶柱和凝胶柱色谱进行分离、纯化,根据波谱技术鉴定结构,研究钩枝藤茎中的化学成分。结果发现:分离得到5个已知二氢黄烷类化合物(-)-表儿茶素-3-没食子酸酯(1)、3,3',5,5',7-五羟基黄烷(2)、(-)-儿茶素(3)、(-)-表儿茶素(4)、(-)-表没食子酸儿茶素(5)和1个酚类化合物(3,5-二甲氧基-4-羟基苯酚)-1-O-β-D-(6-O-没食子酸)葡萄糖苷(6)。其中化合物1、2、4-6均为首次从该植物分离得到。     

蒙药材瑞香狼毒的化学成分研究

采用溶剂提取及柱色谱等方法,首次对瑞香狼毒Stellera chamaejasme L.的正丁醇萃取部位进行系统研究,分离得到6个苯丙素类化合物,并运用UV、1H NMR、13C NMR等现代波谱技术依次鉴定为伞形花内酯7-O-β-D-吡喃木糖(1→6)-β-D-吡喃葡萄糖苷(1),芥子醇1,3’-双-O-β-D-吡喃葡萄糖苷(2),紫丁香苷(3),(+)-落叶松树脂醇4,4’-O-β-D-吡喃葡萄糖苷(4),(+)-松树脂醇4,4’-O-双-β-D-吡喃葡萄糖苷(5)和(+)-丁香树脂醇-双-O-β-D-吡喃葡萄糖苷(6)。其中,化合物4、6为首次从该药材中分离得到。     

羊角拗根的化学成分研究

应用柱色谱技术从羊角拗[Strophanthus divaricatus(Lour.)Hook.et Arn.]根的甲醇提取物中分离纯化出6个化合物,通过1H NMR和13C NMR等波谱技术并对照文献,鉴定其分别为(+)-pinoresinol(1)、沙门苷元(2)、17βH-沙门苷元(3)、羊角拗苷(4)、β-谷甾醇(5)和胡萝卜苷(6)。其中,化合物2~4均为强心苷类化合物。化合物1和3为首次从该植物中分得。     

Daphnodorin dimers from Edgeworthia chrysantha with α-glucosidase inhibitory activity

Four daphnodorin dimers named herein as edgechrins A–D (1–4) were isolated from the stems and twigs of Edgeworthia chrysantha Lindl. along with four known compounds identified as daphnodorin A (5), B (6), C (7) and I (8). Their structures were established by spectroscopic methods, including IR, HR-MS, and 1D- and 2D NMR. All of these compounds showed potent in vitro α-glucosidase inhibitory activity with IC₅₀ values in the range of 0.4–20 μM.     

Effects of daphnodorin A, daphnodorin B and daphnodorin C on human chymase-dependent angiotensin II formation.

We investigated whether daphnodorin A, daphnodorin B and daphnodorin C inhibited human chymase-dependent angiotensin II-forming activity. Although the structures of these compounds are very similar, daphnodorin A completely inhibited angiotensin II formation generated by chymase, while daphnodorin B partially inhibited and daphnodorin C did not. On the other hand, these daphnodorins did not affect angiotensin converting enzyme-dependent angiotensin II formation. Furthermore, these daphnodorins did not inhibit purified human tryptase, which, like chymase, is contained in mast cells. Therefore, daphnodorin A, but not daphnodorin B and daphnodorin C, may specifically inhibit the chymase-dependent angiotensin II formation, and such differences between inhibitory effects of these compounds to human chymase may be useful for the development of human chymase inhibitor.     

Inhibitory mechanism of daphnodorins for human chymase

We investigated the inhibitory mechanisms of daphnodorins for human chymase using three-dimensional molecular modeling. In daphnodorin A-human chymase complex, daphnodorin A was fixed to the active site via hydrogen bonds with Ala177, Phe29, and Gly199 in human chymase, and it formed hydrogen bonds with Ser182 and Gly180, and this complex was formed stably. In daphnodorin B-human chymase complex, daphnodorin B formed hydrogen bonds with Lys28 and Phe29 in human chymase, but it could not form hydrogen bonds with Gly199, Ala177, and Lys179. The phenyl group of daphnodorin B shifted from the P1 hole in human chymase in comparison with that of daphnodorin A. For the inhibition of human chymase by daphnodorins, we indicated that it was significant whether daphnodorins formed hydrogen bonds with Ala177 located in the P1 hole, Ser182 located in the active site, Gly180 located in the anion hole, and with Gly199, Phe29, and Lys28 in human chymase.     

Effects of a new 12-lipoxygenase inhibitor, daphnodorin A, on angiotensin II-induced vascular contraction in hamster.

We studied whether lipoxygenase inhibition suppressed angiotensin II-induced vascular contraction. In the present study, we used a new 12-lipoxygenase inhibitor, daphnodorin A, and an analogue of daphnodorin A, daphnodorin B, which has no inhibitory effects on 12-lipoxygenase. Daphnodorin A at 30 microM and 100 microM significantly suppressed the contractile responses induced by angiotensin 11 (3 x 10(-8) M) in isolated hamster aorta, while daphnodorin B up to 100 microM did not affect the responses. These results suggest that daphnodorin A, but not daphnodorin B, may suppress angiotensin II-induced vascular contractile responses through the inhibition of 12-lipoxygenase.     

Biflavonoids from Fumana procumbens (Dunal) Gren. & Godr

The first phytochemical investigation on the aerial parts of Fumana procumbens (Dunal) Gren. & Godr. led to the isolation and identification of six compounds, including two biflavonoids, i.e. dihydrodaphnodorin B (1) and daphnodorin B (2); three flavonoids, i.e. quercitrin (3), myricitrin (4), and quercetin (5); and a flavan derivative, i.e. epigallocatechin (6). The structures of the compounds were elucidated by extensive 1D- and 2D-NMR spectroscopic analysis in combination with MS experiments. This is the first report on the isolation of biflavonoids from the genus Fumana and from the family Cistaceae.     

Antibacterial metabolites from the Actinomycete <Emphasis Type="Italic">Streptomyces</Emphasis> sp. P294

The Actinomycete strain P294 was isolated from soil and identified as Streptomyces sp. based upon the results of 16S rRNA sequence analysis. Three compounds obtained from the solid fermentation products of this strain have been determined by 1D, 2D NMR and HRMS experiments. These compounds include two new compounds angumycinones C (1) and D (2), and the known compound X-14881 E (3). All compounds were assayed for antibacterial and nematicidal activity. The results showed the three compounds had different degrees of inhibitory activity against several target bacteria but no significant toxicity against the nematode Caenorhabditis elegans.     

Flavonoids and andrographolides from Andrographis paniculata

Two flavonoids, identified as 5,7,2',3'-tetramethoxyflavanone and 5-hydroxy-7,2',3'-trimethoxyflavone, as well as several other flavonoids, andrographolide diterpenoids, and polyphenols, were obtained from the phytochemical investigation of the whole plant of Andrographis paniculata, a well known medicinal plant. The structures of these compounds were established with the aid of spectroscopic methods, including analysis by 2D NMR spectroscopy.     

Flavonoid dimers from the aerial parts of Conyza stricta

A new flavonoid dimer, strictatin (10), was identified from the aerial part of Conyza stricta, in addition to eleven known compounds. The structure of compound 10 was determined by spectral analyses, including 1D, 2D NMR and HR-ESI-MS experiments. All compounds were identified from Conyza stricta for the first time and flavonoid dimers were firstly reported from the family Asteraceae. The chemotaxonomic significance of the identified metabolites was discussed.     

Sesquiterpenoids and steroids from gorgonian Echinogorgia sassapo reticulate

Three guaiane-type sesquiterpenoids (1−3) and five steroids with 3β-hydroxy-5-en-7-one moiety (4−8) were obtained from gorgonian Echinogorgia sassapo reticulata collected from the South China Sea. Among them, new compound 1 was identified as (1S,5S,8S)-8-methoxy-menelloide B by spectroscopic methods including IR, HRESIMS, 1D and 2D NMR, and ECD spectra. All compounds except for 3 were obtained for the first time from the genus Echinogorgia. The chemotaxonomic significance of these compounds was summarized.