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1.
本文对皱纹盘鲍“脓疱病”的病原菌形态和生物学特性研究结果作了报道。这种病原菌细胞呈革兰氏阴性杆状(有时为短杆),大小为0.6-0.7×1.2-1.5μm以单根极生鞭毛运动,氧化酶阳性,接触酶阳性,对0/129(2,4-二氰-6,7-二异丙基喋啶)(150μg)敏感,又根据对葡萄糖的O/F测定为发酵型产酸产气,能还原硝酸盐到亚硝酸盐,V-P反应阴性,精氦酸双水解酶阳性,不液化明胶,不水解淀粉,可在1%-7%NaCl的肉汁培养基中生长,生长温度范围在15-42℃,对低浓度0/129(10mg)不敏感,阿拉伯糖阳性,木糖、肌醇阴性等鉴别特征,可确定其为河流弧菌Ⅱ(Vibrofluvialis-Ⅱ)。  相似文献   

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用戊肝病毒(HEV)基因组编码氨基酸序列1-901-914/2-515-530、3-91-123、2-613-654相应的三段合成多肽为抗原、研制出一种检测抗-HEVIgG的ELISA试剂。以该试剂检测中国、缅甸、印度和前苏联肠道传播非乙型肝炎(ET-NANBH)病人血清105份,仅3份中国病人血清阴性,阳性率为97.1%;检查实验感染HEVL赤猩猩血清,感染前阴性,感染后阳性;检查正常人血清99  相似文献   

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单纯疱疹病毒(HSV)Ⅰ型及Ⅱ型之间有很多共同抗原,能引起血清学交叉反应,鉴别诊断比较困难。本实验利用重组DNA技术,将部分HSV-2DNA的PstI片段克隆到载体质粒PSK中,并筛选出两个重组质粒(P和P)只与HSV-2反应,与HSV-1不反应,这两个重组质粒中所含的HSV-2DNA片段大小分别是3.1和4.3kb,另外,还筛选了一个重组质粒(PHSV2-1,含5.8kbHSV-2DNA片段)与HSV-1和HSV-2均反应。将4.3kb的片段用光生物素标记后作为探针检测了159份人阴道拭子,其中23份样品呈阳性反应,其余均为阴性,从23份阳性样品中挑选12价涂片用间接荧光抗体法检测也都呈阳性反应,随机挑选的几份杂交反应阴性样品在间接荧光试验中也是阴性。本实验制备的HSV通用及HSV-2型特异性探针将比常规的血清学方法诊断和鉴别HSV-1和HSV-2感染更为可靠。  相似文献   

4.
腹泻便中克吕沃氏菌的分离鉴定及致病性研究   总被引:2,自引:0,他引:2  
本文报告了自腹泻便分离到的18株克吕沃尔氏菌的生化鉴定。分子遗传学检测,药物敏感试验及致病性研究结果。18株菌中有11株为抗坏血酸克吕沃尔氏菌,7株栖冷克吕沃尔氏菌。其中溶血试验阳性率38.9%;乳鼠灌胃阳性率33.3%;Helo-2细胞痍变22.2%;兔肠袢结扎阳性5.6%。豚鼠角膜侵袭试验及Vero细胞病变均呈阴性。以此证明,克吕沃尔氏菌中有部分菌株可致生物模型病变,与人腹泻有密切关系。该组细  相似文献   

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腹泻便中克吕沃尔氏菌的分离鉴定及致病性研究   总被引:1,自引:0,他引:1  
本文报告了自腹泻便分离到的18株克吕沃尔氏菌的生化鉴定。分子遗传学检测,药物敏感试验及致病性研究结果。18株菌中有11株为抗坏血酸克吕沃尔氏菌,7株栖冷克吕沃尔氏菌。其中溶血试验阳性率38.9%;乳鼠灌胃阳性率33。3%;Helo-2细胞病变22.2%;兔肠袢结扎阳性5.6%。豚鼠角膜侵袭试验及Vero细胞病变均呈阴性。以此证明,克吕沃尔氏菌中有部分菌株可致生物模型病变,与人腹泻有密切关系。该组细菌的抗药性已很广,临床可用药物不多,一旦发生感染,治疗将十分困难。  相似文献   

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将不同灭活条件下制备的无细胞百日咳毒性试验参考苗进行了检测。结果表明,以浓度0.1%Formalin溶液25℃灭活96-120小时制备的无细胞百日咳菌苗毒性试验参考苗,凝集效价仍可达到百日咳Ⅰ相血清原效价;不耐热毒素试验呈阴性;毒性试验BWDU/ml为75.9-128.4;LPU/ml为2.1-6.0;HSU/ml为3.9-5.7;稳定性良好。该苗可标化作为无细胞百日咳菌苗毒性试验的参考标准  相似文献   

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应用抗人结肠癌单克隆抗体CL-2,CL-4,对205例大肠癌及癌旁病变进行了免疫组化研究。CL-2相应抗原在移行粘膜,轻、中、重度非腺瘤异型增生,大肠癌的阳性率分别为37.6%、63.2%、86.7%、90.9%及86.8%,阳性率呈递增趋势;CL-4相应抗原的阳性率依次是39.1%、57.9%、73.3%、81.8%及77.6%;40例正常大肠粘膜均阴性。结果表明,CL-2、CL-4都是对大肠癌阳性率较高的标记物,但用来鉴别癌与异型增生意义不大;一部分大肠癌可能来源于非腺瘤途径;移行粘膜不同程度的肿瘤相关抗原的表达,反映了其潜在的恶性性质,但其程度低于异型增生,文中,对其临床意义进行了讨论。  相似文献   

8.
酵母饲料的营养价值及应用研究   总被引:2,自引:0,他引:2  
孙建义  许梓荣 《生物技术》1995,5(1):30-32,35
本文报道了以热带假丝酵母ZAU-1为生产菌株,应用固体发酵技术生产的酵母饲料的营养价值,并通过对比饲养试验评价应用效果。试验结果表明,粗蛋白提高11.97%,粗脂肪提高2.44倍;赖氨酸、蛋氨酸和苏氨酸分别提高80.00%、34.07%和69.42%;维生素B2提高11.41倍,B5和B6分别提高19.57%和68.84%;维生素E提高63.43倍,还含有维生素A和D3用多种微量元素。饲养试验结果  相似文献   

9.
长吻鮠碱性磷酸酶的动力学研究   总被引:1,自引:0,他引:1  
采用生化手段,从长吻(LeiocassislongirostrisGunther)肝中提取出碱性磷酸酶(AKP)。提纯倍数为62.08倍,比活为66.43单位/mg蛋白,提取酶液经PAGE和SDS-PAGE只呈现一条区带。该酶的最适pH为10.05,7.0>pH>11.0时不稳定;最适温度为40℃,;对热不很稳定;以磷酸苯二钠为底物其Km值为1.82×10 ̄(-3)mol/L。Mg ̄(2+)为该酶的激活剂,L-Cys、KH_2PO_4、DFP、ME、EDTA-Na_2为抑制剂。选用KH_2PO_4,和DFP作抑制类型的判断,结果表明,KH_2PO_4,属竞争性抑制剂,其抑制常数为2.41mmol/L,DFP为非竞争性抑制剂,抑制常数为1.01mmol/L。  相似文献   

10.
采用固相法合成HIV-1和HIV-2两个多肽,建立了用混合多肽为包被抗原检测HIV-1和HIV-2感染的间接酶联免疫吸附法。检测46份抗HIV-1和HIV-2抗体阳性血清标本以及94份对照血清标本,与UBI试剂比较,其阳性符合率为97.8%,阴性符合率为100%,总符合率为99.3%。实验结果表明,此法可用于HIV-1和HIV-2感染的检测。  相似文献   

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Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.  相似文献   

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Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.  相似文献   

17.
Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.  相似文献   

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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases  相似文献   

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