高血压病患者转铁蛋白与β2-微球蛋白和微量白蛋白的关系

目的:探讨原发性高血压痛患者尿液中β2-微球蛋白(β2-MG)尿微量白蛋白(mALB)和尿转铁蛋白(UTRF)的关系.方法:采用免疫比浊法检测尿β2-MG、mALB和UTRF.结果:mALB和β2-MG之间有显著的正相关关系(F=0.507,p＜0.001),mALB和UTRF之间有显著的正相关关系(F=0.791,p＜0.001),UTRF和B2-MG之间有显著的正相关关系(F=0.554,p＜0.001);单因素LinearRegression分析发现UTRF是mALB和B2-MG的相关因子(p＜0.001).结论:UTRF升高的高血压病患者,mALB和β2-MG均增加,UTRF、mALB和B2-MG的测定可作为高血压患者早期肾损伤的一个理想的反应肾小球滤过功能的敏感指标.     

人β2-微球蛋白基因克隆及其在大肠杆菌中的高效表达

β₂-微球蛋白(β₂m)是主要组织相容性复合体(MHC)Ⅰ类分子的轻链部分, 为制备MHC-Ⅰ类分子四聚体的必要成分。 根据已报道的序列设计特异引物, 利用RT-PCR方法从人白细胞中克隆了β₂m基因, 并构建了成熟β₂m的原核表达载体, 在大肠杆菌中得到高效表达。 表达的β₂m大部分在包涵体中,经洗涤、变性和复性,并以强阴离子交换柱层析纯化,获得SDS-PAGE纯的人重组β₂m,Western印迹法分析表明该蛋白具有与抗人天然β₂m抗体反应的特性。此工作为制备MHC-Ⅰ类分子四聚体奠定基础。     

72例糖尿病血清,尿β2微球蛋白临床分析

７２例糖尿病血清、尿β２微球蛋白临床分析蓝红林梁笑芳高玲桂林医学院附属医院５４１００１血清β２—微球蛋白（β２—Ｍ）的测定作为肾病早期肾功能减退是一项较为敏感的指标之一已有大量临床报道［１］。本组资料为住院治疗的老年人糖尿病及非糖尿病的老年人、中年人...     

尿微量白蛋白检测在社区糖尿病患者管理中的临床意义

目的:观察尿微量白蛋白在糖尿病患者中的改变情况,探讨提高社区糖尿病肾病早期诊断检出率的方法。方法:选择社区2型糖尿病患者73例,根据空腹血糖控制情况及糖尿病病程时长分别分组,比较不同组间患者尿微量白蛋白水平及阳性病例检出率,同时进行相关性分析。结果:MAlb在不同血糖控制情况患者中,血糖水平与MAlb数值、阳性检出率具有正相关性(P<0.05);血糖控制良好患者与其他血糖控制情况患者相比,差异具有(高度)统计学意义(P<0.01;P<0.05);MAlb在不同病程患者中,病程时间与MAlb数值、阳性检出率具有正相关性(P<0.05);≤5年组患者与5～10年组和≥10年组相比,差异具有(高度)统计学意义(P<0.01;P<0.05)。结论:尿微量白蛋白(MAlb)可作为早期糖尿病肾病检测的重要指标,能够提示肾脏病变的发展,定期检查能够提高早期糖尿病肾病的检出率。     

尿微量白蛋白检测在社区糖尿病患者管理中的临床意义

目的：观察尿微量白蛋白在糖尿病患者中的改变情况,探讨提高社区糖尿病肾病早期诊断检出率的方法。方法：选择社区2型糖尿病患者73例,根据空腹血糖控制情况及糖尿病病程时长分别分组,比较不同组间患者尿微量白蛋白水平及阳性病例检出率,同时进行相关性分析。结果：MAIb在不同血糖控制情况患者中,血糖水平与MAIb数值、阳性检出率具有正相关性（P〈0．05）;血糖控制良好患者与其他血糖控制情况患者相比,差异具有（高度）统计学意义（P〈0．01;P〈0．05）;MAIb在不同病程患者中,病程时间与MAIb数值、阳性检出率具有正相关性（P〈0．05）;≤5年组患者与5～10年组和≥10年组相比,差异具有（高度）统计学意义（P〈0．01;P〈0．05）。结论：尿微量白蛋白（MAIb）W作为早期糖尿病肾病检测的重要指标,能够提示肾脏病变的发展,定期检查能够提高早期糖尿病肾病的检出率。     

微量白蛋白尿与2型糖尿病合并代谢综合征的相关性

目的:探讨2型糖尿病(T2DM)患者微量白蛋白尿与代谢综合征(MS)的相关性。方法:282例T2DM患者根据是否合并MS分为MS组(163例)和非MS组(119例),测定24h尿白蛋白(UAlb)及相关生化指标,比较两组UAlb水平及糖尿病肾病(DN)患病率,采用多元Logistic回归方法分析T2DM患者微量白蛋白尿的危险因素。结果:MS组的UAlb及DN患病率明显高于非MS组,且随着MS组分增加,UAlb水平显著升高。多元Logistic回归分析表明甘油三酯、糖化血红蛋白、收缩压为影响UAlb的独立危险因素。结论:T2DM患者微量白蛋白尿与MS密切相关,需采取综合干预措施避免或延缓DN的发生发展。     

微量白蛋白尿与2型糖尿病合并代谢综合征的相关性

目的:探讨2型糖尿病(T2DM)患者微量白蛋白尿与代谢综合征(MS)的相关性.方法:282例T2DM患者根据是否合并MS分为MS组(163例)和非MS组(119例),测定24h尿白蛋白(UAlb)及相关生化指标,比较两组UAlb水平及糖尿病肾病(DN)患病率,采用多元Logistic回归方法分析T2DM患者微量白蛋白尿的危险因素.结果:MS组的UAlb及DN患病率明显高于非MS组,且随着MS组分增加,UAlb水平显著升高.多元Logistic回归分析表明甘油三酯、糖化血红蛋白、收缩压为影响UAlb的独立危险因素.结论:T2DM患者微量白蛋白尿与MS密切相关,需采取综合干预措施避免或延缓DN的发生发展.     

CysC,mALB和尿BGM联合检测在2型糖尿病早期肾损中的临床价值

目的:探讨CysC、mALB和尿BGM联合检测在2型糖尿病早期肾损中的临床价值。方法:选取2010年11月至2013年6月我院内分泌科收治的130名2型糠尿病患者,根据空腹血糖值的不同程度将其分为A、B两组,A组72例,B组58例,另选取同期在我院体检的年龄、性别等资料与之匹配的64名健康者设为C组。分别对其CysC、mALB和尿BGM进行测定,并进行对比分析。结果:通过分析可知,A组和B组的Cys C分别为(3.29±1.09)mg/L、(2.86±1.05)mg/L,相对于C组的(0.87±0.99)mg/L,均明显升高,但A组升高更加明显;A组、B组、C组的mALB分别为(37.36±4.82)mg/L、(36.55±4.31)mg/L、(6.61±3.84)mg/L,B组高于A组,C组高于A组和B组;三组的BGM中,以A组最高,为(634.15±55.24)μg/L,B组其次,为(626.92±48.18)μg/L,均高于C组的(57.12±11.75)μg/L;Cys C、mALB、BGM等阳性指标的检出率分别为76.15%、77.69%、72.31%,而Cys C、mALB、BGM联合检测的检出率为93.85%,明显高于其他单指标检测。且P0.05,差异具有统计学意义。结论:对CysC、mALB和尿BGM进行联合检测对2型糖尿病早期肾损患者具有较高的检出率,相对于单指标检测,敏感性更高。     

2型糖尿病患者尿微量白蛋白水平与动脉粥样硬化的相关性研究

目的:研究2型糖尿病患者尿微量白蛋白(MAU)水平与动脉粥样硬化的相关性。方法:抽样选取2015年2月至2017年2月我体检中心检测的2型糖尿病患者500例,根据临床数据中MAU水平的不同分为异常组188例和正常组312例。分别对比两组患者的临床资料以及生化指标水平,并采用多因素Logistic回归分析法分析患者MAU异常的危险因素。结果:异常组患者的病程、BMI、空腹血糖、甘油三酯、腰臀比均高于正常组,而高密度脂蛋白水平低于正常组(P0.05)。多因素Logistic回归分析显示影响2型糖尿病患者MAU异常的危险因素包括病程(较长)、BMI(较大)、收缩压(较高)、糖化血红蛋白(较高)、高密度脂蛋白(较低)(P0.05)。结论:2型糖尿病患者的病程、BMI、收缩压、糖化血红蛋白、高密度脂蛋白均与MAU的发生密切相关,临床上可通过检查以上指标预测评估患者的病情变化以及预后情况。     

2种方法在血清β2-微球蛋白检测中的效果对比

目的:探讨散射免疫法和荧光免疫层析法在血清β2-微球蛋白中的检测效果对比观察及精确度。方法:选取2016年10月-2017年8月我院收治的肝肾功能损伤患者88例,对患者分别应用散射免疫法以及荧光免疫层析法进行检测。对比两组方法血清β2-微球蛋白的检测结果、灵敏度、特异性以及精确度。结果:检测结果方面,荧光免疫分析法患者的检测率明显高于散射免疫法,漏检率明显低于散射免疫法(P0.05);荧光免疫层析法的灵敏度、特异性以及诊断符合率明显高于散射免疫法(P0.05);各项指标水平方面,患者血清β2-微球蛋白、肌酐、尿酸以及尿素等指标的水平明显高于正常水平(P0.05);精确度方面,荧光免疫层析法批间、批内的精确度明显高于散射免疫法(P0.05)。结论:对患者应用荧光免疫层析法检测血清β2-微球蛋白,有较高的检测符合率、灵敏度以及准确度,能够满足临床检测过程中的要求,进而有利于患者的诊断和治疗,临床上应当进一步推广应用。     

Postthaw survival of rhesus macaque sperm: variation in the response of individual males to different freezing protocols

This study was designed to assess the effects of various cryopreservation protocols on the postthaw survival of rhesus macaque sperm. Multiple ejaculates of five mature males were collected and frozen by each of four different methods. Cryopreservation significantly reduced motility in all samples, regardless of the method, and the response of different ejaculates of the same male to each method proved to be consistent. The freezing method was a significant factor in determining the immediate postthaw motility, although this effect disappeared after 4 hr. Moreover, there was a significant interaction between individual males and freezing method suggesting that developing a single freezing protocol that is universally suitable may be difficult.     

Caloric Restriction Engages Hepatic RNA Processing Mechanisms in Rhesus Monkeys

1. Download high-res image (305KB)
2. Download full-size image

     

Successful implementation of intestinal endoscopy in non‐human primates prompts the possibility of achieving AIDS longitudinal intestinal research

HIV infection induces pathological changes in the intestinal mucosa. Here, a successful endoscopy was performed on the colon of a Chinese rhesus macaque by using Olympus CV170 gastroscope. The stability on postoperative recovery and the integrity of biopsy tissue implied a possibility of achieving AIDS longitudinal intestinal research on macaques.     

In vitro T lymphopoiesis: A model system for stem cell gene therapy for AIDS

Abstract: Stable introduction of therapeutic genes into hematopoietic stem cells has the potential to reconstitute immunity in individuals with HIV infection. However, many important questions regarding the safety and efficacy of this approach remain unanswered and may be addressed in a non-human primate model. To facilitate evaluation of expression of foreign genes in T cells derived from transduced hematopoietic progenitor cells, we have established a culture system that supports the differentiation of rhesus macaque and human CD34⁺ bone marrow derived cells into mature T cells. Thymic stromal monolayers were prepared from the adherent cell fraction of collagenase digested fetal or neonatal thymus. After 10–14 days, purified rhesus CD34⁺ bone marrow-derived cells cultured on thymic stromal monolayers yielded CD3⁺CD4⁺CD8⁺, CD3⁺CD4⁺CD8^?, and CD3⁺CD4^?CD8⁺ cells. Following stimulation with mitogens, these T cells derived from CD34⁺ cells could be expanded over 1,000-fold and maintained in culture for up to 20 weeks. We next evaluated the ability of rhesus CD34⁺ cells transduced with a retroviral vector containing the marker gene neo to undergo in vitro T cell differentiation. CD34⁺ cells transduced in the presence of bone marrow stroma and then cultured on rhesus thymic stroma resulted in T cells containing the retroviral marker gene. These studies should facilitate both in vitro and in vivo studies of hematopoietic stem cell therapeutic strategies for AIDS.     

Toward developing a genome-wide microsatellite marker set for linkage analysis in the rhesus macaque (Macaca mulatta): identification of 76 polymorphic markers

Linkage analysis can be problematic in humans because of the lack of large, multigenerational pedigrees and the difficulties in obtaining phenotypic data on all family members. In contrast, large, captive colonies of rhesus macaque are a potentially valuable resource for linkage studies because detailed phenotypic and genealogical data are kept, inbreeding is avoided, and DNA samples can usually be obtained. Microsatellite marker sets for genome-wide screening are available in a number of species, but not for the rhesus macaque. We tested primers to 400 human microsatellite markers from a genome-wide mapping set using DNA from nine unrelated female rhesus macaques. We found that 76 (19%) of the primers amplified a polymorphic product using the standard protocols for human DNA. The average heterozygosity of the markers in humans was 0.80, compared to 0.65 in the rhesus macaques. This study provides preliminary data, which could be used toward the development of a linkage mapping set in this species. There would be a need, however, to confirm the Mendelian inheritance of the markers.     

Hormonal Correlates of Maternal Style in Captive Macaques (Macaca fuscata and M. mulatta)

Previous studies on nonhuman primate maternal styles showed that variation in infant-directed behavior falls along two dimensions, traditionally labeled as protectiveness and rejection. We investigated maternal styles in rhesus (Macaca mulatta) and Japanese (M. fuscata) macaques living in analogous captive social groups, and correlated them with the levels of estrogen metabolites excreted in female feces during the perinatal period. We focally observed mother-infant pairs 3 hours per week during the first 12 weeks of infant life. We collected fecal samples twice a week from each mother, starting 4 weeks before delivery and ending 4 weeks after delivery. Three significant components were extracted by principal component analysis from 15 different infant directed behaviors, each reflecting a different aspect of mothering style: rejection is correlated with behavioral measures of maternal opposition to contact; protectiveness is correlated with maternal solicitude; and independence is correlated with time in contact, grooming and cradling. Levels of estrogen metabolites are significantly correlated to rejection but not to protectiveness or independence, indicating that, in nonhuman primates, individual endocrine changes related to maternal behavior might specifically affect the timing of contact interruption.