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1.
基于ISSR标记的烤烟种质遗传多样性研究   总被引:47,自引:0,他引:47  
杨本超  肖炳光  陈学军  石春海 《遗传》2005,27(5):753-758
利用ISSR标记分析了24份代表性烤烟种质的遗传多样性。从100个ISSR引物中筛选出10个引物,通过聚丙烯酰胺凝胶电泳可以检测到208条稳定的条带,片段大小介于200~2 400 bp之间,条带数在7~37条之间;扩增片段中多态性带141条,平均多态性比率(PPB)为67.79%。 通过UPGMA聚类分析,24个烤烟品种分为5类,最大一类有12个材料,主要衍生于Coker319。品种间遗传相似指数(GS)范围为0.66~0.85,表明其遗传多样性较低,需要拓宽烤烟种质的遗传基础。同时,利用2个多态性好的ISSR引物可以将这24份烤烟材料区分开,每个品种都有各自独特的指纹图谱,表明ISSR标记适于烟草品种鉴定和遗传多样性研究。  相似文献   

2.
利用ISSR技术对48份乌塌菜种质资源进行遗传多样性分析。从60条随机引物中筛选出稳定性强、条带清晰且多态性丰富的9条引物进行PCR扩增,共扩增出103条谱带,平均每个引物扩增出11.4条带,其中多态性带85条,多态性位点百分率为82.68%。不同乌塌菜种质间遗传相似系数变幅为0.59~0.97,说明ISSR标记能够揭示材料间较高的遗传多样性。利用UPGMA聚类分析,ISSR标记能将48份乌塌菜品种完全区分开,48份乌塌菜种质被划分为4个类群,聚类结果与叶片颜色相关,为乌塌菜品种资源的研究利用提供参考。  相似文献   

3.
不同龙眼资源遗传多样性的SCoT和ISSR 比较分析   总被引:3,自引:1,他引:2       下载免费PDF全文
应用SCoT和ISSR标记对36份龙眼资源和1份近缘种龙荔的遗传多样性进行分析。结果表明:12对SCoT引物共扩增出127条带,平均每条引物扩增10.58条带;15条ISSR引物共扩增出117个条带,平均扩增7.8条带。UPGMA聚类结果表明:SCoT标记和ISSR标记分别在相似系数0.672和0.685水平上,均可将37份材料分成6大类群,SCoT和ISSR标记均适用于龙眼材料的遗传多样性分析,如果将两种标记的数据进行综合分析,可以缩小单一标记的误差。研究结果为龙眼种质资源的保存和利用提供了重要的依据。  相似文献   

4.
利用SRAP和ISSR分子标记,研究了14份耐盐茄子种质资源的遗传多样性,结果表明,2种标记均能揭示材料间较高的遗传多样性,其中ISSR标记多态性略高于SRAP标记。在SRAP分析中,每对引物组合可扩增出8-15条DNA片段,平均为12.12条:26对SRAP引物组合共扩增出315条DNA片段,其中263条具有多态性,多态性比率为83.49%;材料间遗传相似系数变化范围为0.212~0.923,平均值为0.755。在ISSR分析中,每个引物可获得5~16条DNA片段,平均为10.87条;15个ISSR引物共扩增出163条DNA片段,其中141条具有多态性,多态性比率为86.50%;材料间遗传相似系数变幅为0.333-0.957,平均值为0.736。聚类分析表明,2种标记都能将供试材料完全区分开来,聚类结果具有一定的相似性,但也存在明显差异。Mantel相关分析表明,SRAP分析与ISSR分析的相关性达到极显著性水平(r=0.904,P〈0.01)。  相似文献   

5.
基于ISSR标记的中国芋种质资源遗传多样性分析   总被引:1,自引:0,他引:1  
本研究利用ISSR分子标记技术,对国家种质武汉水生蔬菜资源圃收集并保存的来自全国各地的72份芋种质资源进行遗传多样性检测。研究结果表明,13条ISSR引物在72份芋种质资源中共扩增出109条带,其中85条带具有多态性,平均多态性位点比率为78.51%;不同芋种质资源间遗传相似系数的变幅为0.56~1,说明ISSR标记能够揭示芋种质资源之间较高的遗传多样性;在遗传系数0.725处,72份芋种质资源被聚为3大类,为进一步研究芋种质资源分类、起源、保存和利用奠定了基础。  相似文献   

6.
虎杖种质资源的分子标记研究   总被引:2,自引:0,他引:2  
本文应用RAPD、ISSR和SRAP标记对26份虎杖种质资源遗传多样性进行检测.在22个引物中有17个引物(77.3%)扩增产物具多态性,多态性水平相对较高.22个引物共得到98条扩增DNA片段,其中90.8%具有多态性.每个多态性引物平均可扩增出5.24个多态性片段.聚类分析表明,利用BAPD、ISSR和SRAP技术相结合可将全部供试材料区分开,26份材料在Gs值0.54水平上全部聚为一类,以所有材料间的平均遗传相似遗传系数0.71为阈值,将其分为11类.虎杖种质资源在分子水平上确实存在较大遗传差异,RAPD、ISSR和SRAP标记可作为构建虎杖DNA指纹图谱的有效工具.  相似文献   

7.
利用ISSR分子标记对7种12居群的川产淫羊藿属植物进行遗传多样性及亲缘关系分析。结果发现23个ISSR随机引物共扩增194条清晰条带,其中169条具多态性,平均多态性位点比率为87.11%,有效等位基因数Ne=1.534 2,Nei基因多样性指数H=0.314 4,Shannon多样性指数I=0.469 7,表明物种间遗传多样性丰富。7种川产淫羊藿属植物间的遗传相似系数为0.653 2~0.748 9,聚类分析和主成分分析直观的显示出12份供试材料间的亲缘关系,并将其分为两类。表明ISSR分子标记技术可以用于川产淫羊藿属植物的遗传多样性和亲缘关系分析。  相似文献   

8.
运用ISSR标记对采自云南的25份野生种质和4份驯化新品系中型狼尾草材料进行遗传多样性分析。结果显示:(1)50条ISSR引物中共筛选出10条能扩增出清晰条带且多态性明显的引物,29份材料DNA共获得72个扩增位点,其中多态性位点62个,多态性比率为87.4%,平均每条引物扩增位点为7.2个;平均观察等位基因数(Na)、有效等位基因数(Ne)、Shannon多样性信息指数(I)和Nei’s基因多样性指数(H)分别为1.861 1、1.742 8、0.561 0和0.395 9;种质材料间的遗传相似性系数变幅为0.236~0.903,表现出丰富的遗传多样性。(2)利用UPGMA聚类分析,以遗传相似系数0.51为界,29份材料划分为4大类,但Mantel检测表明29份种质材料的遗传聚类和地理距离之间不存在显著的正相关关系(r=0.437 0,P=0.204 6)。研究结果首次从分子水平揭示了中型狼尾草的遗传多样性和变异水平,为合理地引种、驯化、保护和利用中型狼尾草野生资源提供了重要的参考依据和数据支持。  相似文献   

9.
猕猴桃属16个雄性材料遗传多样性的ISSR分析   总被引:1,自引:0,他引:1  
利用ISSR分子标记对雄性猕猴桃16个材料进行遗传多样性分析。从100条引物中筛选出10条引物用于ISSR扩增,共扩增出172条带,其中多态性条带140条,多态性百分率为81.4%;经POPGENE 1.32软件分析结果显示,16个雄性猕猴桃材料的遗传距离在0.1503~0.5128之间,平均Nei's基因多样性指数(H)为0.2416,平均Shannon信息指数(I)为0.4048;聚类分析结果显示,在遗传相似系数为0.64处可将供试材料分成4类,第Ⅰ类为中华和美味猕猴桃,第Ⅱ类为阔叶、毛花猕猴桃,第Ⅲ类为魁绿猕猴桃,第Ⅳ类为四萼猕猴桃。结果表明ISSR可用于雄性猕猴桃遗传多样性研究,该研究结果可为猕猴桃种质资源的进一步开发利用提供重要信息。  相似文献   

10.
利用ISSR标记对33份绿豆种质进行了遗传差异和亲缘关系分析。 结果表明,从46条ISSR引物中筛选出10条扩增条带清晰、多态性高的引物,利用这10条引物从33份绿豆种质中共扩增出118条条带,其中多态性条带115条,多态性位点比例98.18%。遗传相似性与聚类分析结果表明,供试绿豆种质间的遗传相似系数范围为0.50~0.98之间,平均0.68。当遗传相似性系数为0.682时,供试33份绿豆种质资源分为4个ISSR类群( ISSR Groups,IGs),第Ⅰ类群包括产地为黑龙江、吉林共9份和河北的1份绿豆种质资源,第二类群包括河南、山西和陕西的所有和河北的4份绿豆种质资源,第三类群为来自泰国的5份绿豆抗虫资源,第四类群包括山东和内蒙各2份绿豆资源;当遗传相似系数为0.98时,供试的33份绿豆种质资源可被全部区分开。 ISSR分析结果表明, ISSR类群划分与绿豆的地理来源存在一定相关性;而同一类群中,地理来源相同的绿豆种质间也存在一定的遗传差异。  相似文献   

11.
穗花杉ISSR引物反应条件的优化与筛选   总被引:18,自引:2,他引:16  
在研究穗花杉(Amentotaxus aragotaenia)的遗传多样性过程中,为了获得清晰、重复性好ISSR扩增结果,对影响ISSR-PCR的多个因素包括模板浓度、Taq酶的选择和用量、Mg2+和dNTPs浓度及退火温度等指标等进行了筛选和优化,确定了穗花杉ISSR-PCR分析的最适扩增条件: 20 μL PCR反应体系中,2 μL 10×Taq酶配套缓冲液,1.8 U Taq聚合酶(上海生工公司),0.2 μmol·L-1引物,0.18 mmol·L-1 dNTP,1.5~2.5 mmol·L-1 MgCl2,10 ng·μL-1模板DNA。用来自不同居群7个个体,以100个ISSR引物进行PCR扩增,筛选出扩增效果较好的10个引物。得到了92个位点,其中45个多态性位点,多态性位点比例为49%。  相似文献   

12.
The existence of genetic diversity in Crocus sativus has globally remained a mystery till date. The study investigated PCR based DNA amplification profile of saffron using ISSR and RAPD based primers. A total of 38 amplicons were generated by ISSR primers in the range from 7 to 12 with an average of 9.50 bands per primer. 20 bands were found to be polymorphic and 18 were monomorphic with an average percentage of polymorphism as 52.48%. RAPD based amplification revealed a total 161 amplicons, 107 as polymorphic and 54 as monomorphic with an average percentage of polymorphism as 66.44%. Cumulative results of RAPD and ISSR demonstrated that Nei-Li’s similarity index ranged between 0.70 and 0.97. The results of AMOVA has revealed 9% of variance among populations and 91% of variance within populations, Φ PT was found as 0.089, which indicates existence of genetic differences though limited. In conclusion, the results indicate that saffron accessions are minimally genetically differentiated, which could be capitalized in future breeding programmes to ameliorate this precious crop.  相似文献   

13.
To investigate the genetic diversity and phylogenetic relationships between polyploid Leymus and related diploid species of the Triticeae tribe, inter-simple sequence repeats (ISSR) markers was used to analyze 41 Leymus accessions representing 22 species and 2 subspecies, together with Pseudoroegneria stipifolia (St), Psathyrostachys fragilis (Ns), Australopyrum retrofractum (W), Hordeum bogdanii, H. chilense (H) and Lophopyrum elongatum (Ee). A total of 376 clear and reproducible DNA fragments were amplified by 29 ISSR primers, among which 368 (97.87%) fragments were found to be polymorphic. 8–18 polymorphic bands were amplified by each polymorphic primer, with an average of 12.69 bands. The data of 376 ISSR bands were used to generate Nei’s similarity coefficients and to construct a dendrogram by means of UPGMA. The similarity coefficients data suggested great genetic diversity in genus Leymus and related diploid Triticeae species, the genetic diversity among the different species more abundant than that of the different accessions. The dendrogram and principal coordinate analysis showed explicit interspecific relationships and demonstrated close phylogenetic relationships between Leymus species and Psathyrostachys.  相似文献   

14.
Flavoparmelia caperata (L.) Hale is medicinally very important and possesses antifungal and antibacterial activities. F. caperata is the only species found in India. Inter simple sequence repeat (ISSR) and Directed amplification of minisatellite DNA (DAMD) methods were used to analyze the genetic variability within F. caperata from the Western Himalayan region of India. Eleven ISSR and 10 DAMD primers produced 139 and 117 polymorphic bands, and detected 91.44 and 82.34 % polymorphisms, respectively. Cumulative band data generated for ISSR and DAMD markers resulted in 86.86 % polymorphism across all the accessions of F. caperata. The average Polymorphic information content (PIC) value obtained with ISSR, DAMD, and cumulative band data were 0.28, 0.27, and 0.27, respectively. The clustering of the F. caperata accessions in the UPGMA dendrogram showed that these accessions are intermingled with each other in different subclusters irrespective of their geographical affiliations. The pattern of genetic variations within F. caperata accessions could be due to free exchange of spores that might have taken place among these accessions in the wild. ISSR and DAMD markers efficiently and reliably resulted in discrete banding patterns and polymorphic profiles. These markers despite targeting different regions of genome, revealed almost similar levels of polymorphism across all the accessions. The wide range of genetic distance and high level of polymorphism detected by ISSR and DAMD reflected a high genetic variability among the different accessions of F. caperata.  相似文献   

15.
22种木莲属植物亲缘关系的ISSR分析   总被引:1,自引:0,他引:1  
利用ISSR标记分析技术探讨了22种木莲属植物的遗传亲缘关系。选用10对ISSR引物组合对木莲属22个种的基因组进行分析。结果表明:22个种共扩增出595条DNA带,其中多态性条带占94.45%;根据Nei Li遗传相似性系数在0.68处,UPGMA聚类结果将22个种划分为6个类群,在0.70处进一步可分为12个亚类群;22种木莲属植物的Nei-Li遗传相似性系数变化范围为0.605(乳源木莲与球果木莲之间)~0.956(中缅木莲与滇南木莲之间),平均遗传相似性系数为0.698。说明木莲属植物种间差异性大。其中中缅木莲、滇南木莲之间遗传相似性系数为0.956,两者亲缘关系最近。该结果同时支持将巴东木莲、乳源木莲、滇桂木莲分别作为独立的种。  相似文献   

16.
RAPD和ISSR标记对水稻化感种质资源遗传多态性的分析   总被引:23,自引:1,他引:22  
运用RAPD和ISSR技术分析水稻化感种质资源的遗传多态性。从供试材料中筛选到具有多态性的RAPD引物12条,ISSR引物7条。RAPD引物共扩增到85条清晰的多态性条带,多态性条带比率为69.4%。ISSR引物共扩增到34条清晰的多态性条带,多态性条带比率为53.0%。对两种标记结果进行UPGMA聚类分析,结果极其类似,呈极显著的正相关(r=0.74)。聚类结果表明,地理位置相近的品种聚为一类。部分具有较强化感作用潜力的水稻品种亲缘关系很近,表明控制其化感作用性状的基因可能是等位的相同基因。而部分化感作用潜力差异显著的水稻品种聚为一类,这是由于人类在长期高产品种的定向选择过程中,水稻化感作用性状不被注意而丢失,遗传基础日益狭窄的原因。  相似文献   

17.
Iran is an important producer of cornelian cherries (Cornus mas L.). Seed propagation and long term human selection have given rise to a great diversity of trees. In this study, ISSR marker was used for genetic diversity evaluation of 40 accessions of cornelian cherries (C. mas L.). With 20 ISSR primers, 171 polymorphic bands were detected with polymorphism ratio range of 80–100%. The average polymorphism information content (PIC) was 0.46, which shows that the majority of primers are informative. The average values of Effective Multiplex Ratio (EMR), Marker Index (MI), effective number of alleles (Ne), Nei's gene diversity (He) and Shannon's information index (I) for all primers were 8.0, 3.75, 1.756, 0.416 and 0.595 respectively. Based on these results, ISSR analysis can be used for the characterization and grouping of cornelian cherry accessions. This is the first study demonstrating that ISSR analyses can be used to differentiate and classify cornelian cherry accessions.  相似文献   

18.
Salvia miltiorrhiza is one of the most important traditional Chinese medicinal plants for its therapeutic effects. In the present study, morphological traits, ISSR (inter-simple sequence related) and SRAP (sequence-related amplified polymorphism) markers were used to analyze the genetic diversity of 59 S. miltiorrhiza phenotypes. Out of the 100 ISSR primers and 100 SRAP primer combinations screened, 13 ISSRs and 7 SRAPs were exploited to evaluate the level of polymorphism and discriminating capacity. The results showed that the 13 ISSRs generated 190 repeatable amplified bands, of which 177 (93.2%) were polymorphic, with an average of 13.6 polymorphic fragments per primer. The 7 SRAPs produced 286 repeatable amplified bands, of which 266 (93.4%) were polymorphic, with an average of 38.1 polymorphic fragments per primer. Cluster analysis readily separated different morphological accessions, wild and cultivated controls based on morphological traits, ISSR and SRAP markers. The study indicated that morphological traits, ISSR and SRAP markers were reliable and effective for assessing the genetic diversity of phenotypic S. miltiorrhiza accessions. The overall results suggested that the introduction of genetic variation from morphology-based germplasms enlarged the genetic base for the collection, conservation and further breeding program of S. miltiorrhiza germplasm.  相似文献   

19.
ISSR and SSR markers were used to evaluate genetic diversity among 33 Cynodon dactylon accessions and 22 cultivars from four different countries in order to provide information on how to improve the utilization of bermudagrass germplasms. Eighty eight bands were amplified by nine SSR primer combinations and 236 bands were observed from 23 ISSR primers. The results showed that 97.7% of the SSR primers and 86.9% of the ISSR primers were polymorphic. The genetic similarity coefficients (GSC), gene diversity (He) and Shannon index (I) were 0.58–0.97, 0.27 and 0.41, respectively, for ISSR and 0.52–0.97, 0.29, and 0.43 for SSR. The UPGMA analysis clustered the 55 accessions (cultivars) into three groups. The cluster results produced by the ISSR data were close to the SSR data results. Analysis based on the combined ISSR and SSR data was more closely related to the geographical distribution of the tested germplasm.  相似文献   

20.
14个蝴蝶兰品种遗传关系的ISSR分析   总被引:3,自引:0,他引:3  
采用ISSR分子标记技术对14个蝴蝶兰品种进行品种间遗传关系的研究。利用14个筛选的引物共扩增出179条带,其中多态性条带147条,多态性条带比率(PPB)为82%。品种之间的遗传相似系数范围在0.734~0.936间,说明部分蝴蝶兰品种间存在显著的遗传分化。14个引物组合可区分所有14个品种,并且检测到20条品种特异性条带,这些品种特异条带可用来鉴定供试蝴蝶兰中的10个品种。因此,ISSR分子标记能有效地进行蝴蝶兰品种鉴定。UPGMA聚类分析表明,14个品种可聚为2类,聚类情况与花色特征比较一致,但与花色的划分结果不完全相同,这可能是由于品种间杂交引起的。本文也论讨了ISSR分析结果对蝴蝶兰育种的指导意义。  相似文献   

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