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1.
Q. Zhang K. D. Liu G. P. Yang M. A. Saghai Maroof C. G. Xu Z. Q. Zhou 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(1-2):112-118
The partial sterility of hybrids between the indica and japonica rice subspecies of Asian cultivated rice is a serious constraint
for utilizing inter-subspecific heterosis in hybrid rice breeding. In this study, we have investigated the relationship between
molecular-marker polymorphism and indica-japonica hybrid fertility using a diallel set involving 20 rice accessions including
9 indica and 11 japonica varieties. Spikelet fertility of the resulting 190 F1s and their parents was examined in a replicated field trial. Intra-subspecific hybrids showed much higher spikelet fertility
than inter-subspecific hybrids except in crosses involving wide-compatibility varieties. The parents were surveyed for DNA
polymorphism using 96 RFLP and ten SSR markers, which revealed extensive genetic differentiation between indica and japonica
varieties. A large number of markers detected highly significant effects on hybrid fertility. The chromosomal locations for
many of the positive markers coincided well with previously identified loci for hybrid sterility. The correlation between
hybrid fertility and parental distance was low in both intra- and inter-subspecific crosses. The results suggest that the
genetic basis of indica-japonica hybrid sterility is complex. It is the qualitative, rather than the quantitative, difference
between the parents that determines the fertility of hybrids.
Received: 3 January 1997/Accepted: 17 January 1997 相似文献
2.
J. Wang K. D. Liu C. G. Xu X. H. Li Q. Zhang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(3):407-412
Wide-compatibility varieties (WCVs) are a special class of rice germplasm that is able to produce fertile hybrids when crossed
to both indica and japonica rice varieties. WCVs may differ greatly in their spectrum and level of compatibility. The objective
of this study was to determine the genetic basis of wide-compatibility conferred by ‘Dular’, a landrace variety from India
that has demonstrated a high level of wide-compatibility in previous studies with a broad range of indica and japonica varieties.
A three-way cross (‘Balilla/Dular//Nanjing 11’) was made and the resulting F1 population evaluated in the field for spikelet fertility. A total of 235 plants from this population was assayed individually
for restriction fragment length polymorphisms (RFLPs) at 159 marker loci covering the entire rice genome at regular intervals.
Quantitative trait locus (QTL) analysis identified 5 loci, located on chromosomes 1, 3, 5, 6 and 8, as having significant
effects on hybrid fertility, which jointly explained 55.5% of the fertility variation in this population. The QTL on chromosome
5 ( f5) showed the largest effect on hybrid fertility, followed by those on chromosomes 6 ( f6), 3 ( f3) and 1 ( f1), with the one on chromosome 8 ( f8) having the smallest effect. Genotypes each composed of an allele from ‘Dular’ and an allele from ‘Nanjing 11’ at four ( f3, f5, f6 and f8) of the five QTLs contributed to the increase of fertility in the population. In contrast, the genotype composed of alleles
from ‘Balilla’ and ‘Nanjing 11’ at the fifth locus ( f1) was in the direction of increasing fertility. Analysis of variance using marker genotypes at the five QTLs as the groups
detected two interactions involving four of the five loci, a 2-locus interaction between f5 and f8 and a 3-locus interaction among f3, f5 and f6. The level of hybrid fertility is the result of complex interactions among these loci. The implication of the present findings
in the utilization of the wide-compatibility of ‘Dular’ in rice breeding programs is also discussed.
Received: 21 October 1997 / Accepted: 30 December 1997 相似文献
3.
Genetic basis of low-temperature-sensitive sterility in indica-japonica hybrids of rice as determined by RFLP analysis 总被引:13,自引:0,他引:13
H. B. Li J. Wang A. M. Liu K. D. Liu Qifa Zhang J. S. Zou 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(7):1092-1097
Low-temperature-sensitive sterility (LTSS) has become one of the major obstacles in indica-japonica hybrid rice breeding.
In this study, we determined, using RFLP markers, the genetic basis of LTSS in two populations derived from crosses between
indica and japonica parents, the BC1F1 of 3037/02428//3037 and the F2 of 3037/02428. The fertility segregation in the two populations under low-temperature conditions was used as a measurement
of the temperature sensitivity of the various genotypes in the populations. A RFLP survey of bulked extremes from the BC1F1 population identified three genomic regions, two on chromosome 1 and one on chromosome 12, that were likely to contain genes
for LTSS (or Ste loci). One-way ANOVA and QTL analysis using a total of 19 markers from these three genomic regions resolved three Ste loci in the BC1F1 population and two Ste loci in the F2 population. On the basis of chromosomal location these loci were distinct from those governing wide-compatibility identified
in previous studies. Two- and three-way ANOVA showed that these loci acted essentially independent of each other in conditioning
LTSS. The main mode of gene action was an interaction between the indica and the japonica alleles within each locus. For each
respective locus this resulted in a drastic fertility reduction in the heterozygote state relative to the homozygote state.
The results have significant implications in indica-japonica hybrid rice breeding programs.
Received : 10 April 1996 / Accepted: 2 June 1997 相似文献
4.
The rice indica/japonica hybrid shows strong heterosis.However,such inter-subspecific hybrid can't be directly used in rice production due to its low spikelet fertility.The S5 locus was proved to be associated with fertility of indica/japonica hybrid and its S5n allele from wide-compatibility variety(WCV)is capable to overcome fertility barrier.In the present study,we reported the causal sites in the S5 locus responsible for compatibility of indica/japonica hybrid.Fine-mapping of the S5 locus using the 11 test-cross families pinpoints a candidate S5 locus encoding aspartic protease(Asp).Intragenic recombination within the Asp gene happened in a number of recombinants,resulting in chimeric S5j-S5n alleles.Just like S5n,the chimeric s5j-S5n allele displayed higher spikelet fertility when combined with the S5i allele.In the complementary test,however,the S5n allele from WCVs failed to enhance fertilities of the indica/japonica hybrids.Compared to both indica and japonica varieties.all nine WCVs from different resources are characterized with a 136 bp deletion in the Asp N-terminus.which probably renders the S5n allele non-functional.Furthermore,an A/C polymorphic site is detected 1,233 bp downstream of the Asp start codon.The heterozygous A/C site of the Asp gene in indica/japonica hybrid is believed to be the casual factorto causc partial sterility.The functional makers based on the two polymorphic sites will be broadly used in developing wide-compatibility rice varieties. 相似文献
5.
Singh SP Sundaram RM Biradar SK Ahmed MI Viraktamath BC Siddiq EA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(3):509-517
Although pronounced heterosis in inter-subspecific hybrids was known in rice for a long time, its utilization for hybrid rice breeding has been limited due to their hybrid sterility (HS). For the last two decades, however, a few inter-subspecific hybrids have been developed by incorporating wide-compatibility genes (WCG) that resolve HS, into parental lines of these inter-subspecific hybrids. For effective use of WCG, it is necessary to find convenient markers linked to WCG of practical importance. In this paper, initially a set of simple sequence repeat (SSR) markers in the vicinity of known WCG loci identified based on comparative linkage maps have been surveyed in a population derived from the three-way cross- IR36/Dular//Akihikari, where a known donor of WCG Dular was crossed to a representative indica and japonica cultivar. Of the five parental polymorphic markers, RM253 and RM276 were found to be closely linked to the WCG locus S5 at a distance of 3.0 and 2.8 cM, respectively. Later, loci for HS were examined in three F2 populations derived from inter-subspecific crosses, with same set of SSR markers. The locus S8 was confirmed to have major influence on HS in the F2 population derived from CHMRF-1/Taichung65 since two SSR markers in its vicinity, RM412 and RM141, co-segregated with HS at a map distance of 7.6 and 4.8 cM, respectively. In the F2 population derived from the cross BPT5204/Taipei309, three SSR markers in the vicinity of S5, RM50, RM276 and RM136 co-segregated with HS at a map distance of 4.2, 3.2 and 7.8 cM, respectively. In the third F2 population derived from Swarna/Taipei309, the SSR markers in the vicinity of S5, RM225, RM253, RM50, RM276 and RM136 were identified to co-segregate with HS at a map distance of 3.2, 2.6, 3.4, 2.6 and 6.6 cM, respectively. These results indicated a clear picture of WCG in Dular as well as the predominant role of HS alleles at S5 locus. The identified SSR markers are expected to be used for incorporation of WCG into parental lines in hybrid rice breeding to solve HS in inter-subspecific hybrids.S.P. Singh , R.M. Sundaram contributed equally 相似文献
6.
Qing Ji a Jufei Lu b Qing Chao a Yan Zhang a Meijing Zhang a Minghong Gu b Mingliang Xu a c a National Maize Improvement Center China Agricultural University Beijing China b Key lab for plant biotechnology of Jiangsu province Yangzhou University Yangzhou China c Key Laboratory of Crop Genetic Improvement China 《Acta Genetica Sinica》2010,(1)
The rice indica/japonica hybrid shows strong heterosis.However,such inter-subspecific hybrid can't be directly used in rice production due to its low spikelet fertility.The S5 locus was proved to be associated with fertility of indica/japonica hybrid and its S5n allele from wide-compatibility variety (WCV) is capable to overcome fertility barrier.In the present study,we reported the causal sites in the S5 locus responsible for compatibility of indica/japonica hybrid.Fine-mapping of the S5 locus using the 11... 相似文献
7.
P. Wu G. Zhang N. Huang J. K. Ladha 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,91(6-7):825-829
Significant segregation of spikelet fertility occurred in an F2 population derived from a spikelet fertility-normal F1 hybrid produced by a cross between Palawan, a japonica variety, and IR42, an indica variety. To identify factors controlling the fertility segregation, we used 104 RFLP markers covering all 12 rice chromosomes to investigate the association of spikelet fertility and marker segregation. We found that the segregation of two sets of gene pairs was significantly (P < 0.001) associated with fertility segregation. The first pair of genes was linked to RFLP marker RG778 on chromosome 12 and RFLP markers RG690/RG369 on chromosome 1. A significant reduction in fertility was observed when the plants were homozygote at RG778 with the indica allele as well as homozygote at RG690/RG369 with the japonica allele. The second pair of genes was linked to RG218 on chromosome 12 and RG650 on chromosome 7, respectively. The recombinant homozygote at these two loci showed a significant reduction on spikelet fertility. The non-allelic interaction effect was further modified by a gene linked to RG778, resulting in even lower fertility. The results of this study provides the first evidence of chromosomal localization of sporophytic sterility genes whose interaction can result in a reduction of spikelet fertility in the F2 derived from fertility-normal F1. 相似文献
8.
Genetic dissection of embryo sac fertility, pollen fertility, and their contributions to spikelet fertility of intersubspecific hybrids in rice 总被引:6,自引:0,他引:6
Song X Qiu SQ Xu CG Li XH Zhang Q 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,110(2):205-211
The partial sterility of hybrids has been a major barrier for utilization of the strong heterosis expressed in hybrids between Oryza sativa ssp. indica and O. sativa ssp. japonica. Wide-compatibility varieties, comprising a special class of germplasm, are able to produce fertile hybrids when crossed to both indica and japonica varieties. However, all the work on wide compatibility and majority of studies on indica/japonica hybrid sterility reported so far were based only on spikelet fertility; thus, it is not known to what extent male and female gamete abortions influence hybrid sterility. In this study, we investigated pollen fertility, embryo sac fertility, and spikelet fertility in an F1 population of 202 true hybrid plants derived from a three-way cross (02428/Nanjing 11//Balilla). A partial regression analysis showed that the pollen and embryo sac fertility contributed almost equally to spikelet fertility. QTL analysis based on a linkage map of 191 polymorphic marker loci identified two QTLs for pollen fertility, one QTL for embryo sac fertility, and three QTLs for spikelet fertility. The S5 locus, previously identified as a locus for wide compatibility by spikelet fertility analysis, is a major locus for embryo sac fertility, and a QTL on chromosome 5 had a major effect on pollen fertility. These two loci coincided with the two major QTLs for spikelet fertility. The study also detected a QTL on chromosome 8, showing a large effect on spikelet fertility but no effect on either pollen or embryo sac fertility. Very little interaction among the QTLs was detected. The implications of the findings in rice breeding programs are discussed. 相似文献
9.
Wang GW He YQ Xu CG Zhang Q 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(2):382-387
Wide-compatibility varieties (WCVs), comprising a special class of rice germplasm, are able to produce fertile hybrids when
crossed with both indica and japonica varieties. Dular, a landrace variety from India, has both a wide spectrum and high level
of wide-compatibility when crossed with a range of indica and japonica varieties. In previous studies, an allele at the f5 locus from Dular (f5-Du) was identified as a neutral allele conferring wide-compatibility with a large effect on both pollen and spikelet fertility.
Using a population of 1993 hybrid plants derived from a cross between ZS(f5-Du/f5-ZS) (F1 of near isogenic line of Zhenshan 97 containing f5-Du) and Balilla (a japonica tester), f5-Du was genetically mapped to an interval of about 1.6 cM, with 0.8 cM from both SSR markers WFPM3 and WFPR1. Combined with
bioinformatic analysis, a contig map was constructed for the f5 region, consisting of five bacterial artificial chromosome (BAC) or P1 artificial chromosome (PAC) clones and spanning approximately
437 kb in length. By assaying the recombinant events in the region with markers developed using the sequence information,
the f5 locus was further narrowed down to a 70 kb fragment containing nine predicted genes. The result will be very useful for cloning
this gene and marker-assisted transferring of the neutral allele in rice breeding programs. 相似文献
10.
S. Fukuoka H. Namai K. Okuno 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(3):446-449
Complementary recessive genes hwd1 and hwd2 controlling hybrid breakdown (weakness of F2 and later generations) were mapped in rice using RFLP markers. These genes produce a plant that is shorter and has fewer
tillers than normal plants when the two loci have only one or no dominant allele at both loci. A cultivar with two dominant
alleles at the hwd1 locus and a cultivar with two dominant alleles at the hwd2 locus were crossed with a double recessive tester line. Linkage analysis was carried out for each gene independently in two
F2 populations derived from these crosses. hwd1 was mapped on the distal region of rice genetic linkage map for chromosome 10, flanked by RFLP markers C701 and R2309 at
a distance of 0.9 centiMorgans (cM) and 0.6 cM, respectively. hwd2 was mapped in the central region of rice genetic linkage map for chromosome 7, tightly linked with 4 RFLP markers without
detectable recombination. The usefulness of RFLP mapping and map information for the genes controlling reproductive barriers
are discussed in the context of breeding using diverse rice germplasm, especially gene introduction by marker-aided selection. 相似文献