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一种改进的阴茎电刺激采精法和猕猴藏酋猴及熊猴的采精及其精液特征的初步研究 总被引:6,自引:2,他引:4
本文报道了一种改进的阴茎电刺激采精法,用脱脂棉和铝箔作为电极,以避免直接用金属电极可能对阴茎的损伤,并运用这一方法对猕猴(Macacamulatta)、藏酋猴(M.thibetana)和熊猴(M.assamensis)进行了电刺激采精及其精液特征研究。电刺激采精模式为连续刺激和间断刺激方式。在采精过程中没有发生阴茎损伤。对初次接受电刺激采精的动物以间断刺激模式效果较好。猕猴、藏酋猴和熊猴的射精体积分别为2.0±0.1、6.3±1.1和3.2±0.6ml;液化体积分别为0.7±0.6、2.1±0.4和1.7±0.3ml;精子浓度分别为12.6±1.2×10~8、45.6±5.6×10~8和11.5±0.9×10~8/ml。3种动物精液的液化率分别为:猕猴36.2±0.9%,藏酋猴34.0±1.4%;熊猴51.8±1.2%。3种动物的精子总数与射精体积和凝块体积没有相关性(r~2=0.079;0.016;0.094和r~2=0.064;0.020;0.072)。上述结果表明:1)改进的阴茎电刺激采精法适用于猕猴,特别是阴茎表面较为粗糙的藏酋猴和熊猴;2)藏酋猴的射精体积和精子总数是迄今已报道的非人灵长类中最大的,可能 相似文献
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根田鼠的最大同化能量 总被引:8,自引:0,他引:8
本文利用逐步降温诱导,以食物平衡法对青海高原的根田鼠(Microtus oeconomus)的最大同化能量进行了测定,设有5个实验温度;23℃,15℃,10℃,5℃和3℃,结果显示每个动物每天能量摄入于 5℃大, 197. 14±8.66KJ;同样,消化能、消化率、同化能和同化率也于 5℃8大,分别为123.96±13.05KJ,63.0±4.8%,121.77±13.52KJ和51.4±5.2%;消化率与同化率3℃最小,分别为51.5±3.9%和50.4±4.0%.根田鼠的最大同化能量为基础代谢率(BMR)的3.2—3.5倍,明显高于以平均每日代谢率(ADMR)估测的每日能耗,故在自然生境中根田鼠的能量收支很少达到其生理上限,其生存价较高. 相似文献
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布氏田鼠静止代谢率特征 总被引:9,自引:3,他引:6
本文以布氏田鼠的耗氧量为指标,探讨了布氏田鼠的能量代谢特征,布氏田鼠的热中性区为27.5至32.5℃,最低静止代谢率为1.836mlW2/g.h,是期望RMR的132.6%,最低热传导率为0.189mlO2/g.h,是期望热传导率的114.5%,环境温度在5-27.5℃范围内,RMR与Ta呈负相关,回归方程为RMR=6.968-0.1852Ts,体温稍有降低。Ta35℃时,RMR升高,体温升高1. 相似文献
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[^3H]Cortisol及[^3H]Dexamethasone在大鼠肝细胞膜上的特异结合位点 总被引:1,自引:0,他引:1
本研究应用[3H]cortisol和[3H]dexamethasone(DEX)两种配基,观察到大鼠肝细胞膜上存在一类糖皮质激素(GC)特异结合位点。这些位点与GC的结合具有饱和性、高亲和力及低容量。其平衡解离常数(Kd)分别为12.84±6.58nmol/L和40.27±23.44nmol/L;最大结合容量(Bmax)分别为2.57±1.84pmol/mg蛋白质与0.64±0.18pmol/mg蛋白质(cortisol,n=4;DEX,n=3;±SE)。动力学实验数据所得的Kd值与Scatchard分析所得的Kd值结果基本一致。[3H]cortisol和[3H]DEX饱和结合实验数据用Scatchard作图分析,均显示为直线。Hill系数则分别为0.9880和0.9990.竞争抑制实验结果表明,cortisol对[3H]cortisol的结合位点有高度特异性竞争,比其它几种类固醇(强的松、黄体酮、RU486、DEX)的竞争力至少强40倍以上.用放射自显影技术进行研究,也提供了[3H]cortisol特异结合银粒位于大鼠肝细胞膜上的依据。 相似文献
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发头裸腹SouMoinairrasaBrehm(Cladocera)在25±1℃下有3个幼龄,6个成龄,平均寿命为167.7h,平均最大体长为1.75mm平均产仔量为117个。rm为1.358/d;在30±1℃下有3个幼龄,7个成龄,平均寿命为121.0h平均最大体长为1.71mm,平均产仔量为106.6个,rm为1.650/d。在生命初期30±1℃下体重增长率(dW/dt),相对体长增长率(dL 相似文献
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磷化氢、二氧化碳混合气体对腐食酪螨成螨的生物学效应 总被引:7,自引:0,他引:7
本文研究结果表明:腐食酪螨Tyrophagusputrescentiae(Schrank)成螨在0%、8%、16%(容积比)CO2气体中耗氧量随CO2浓度的增加而增加,当在32%、64%CO2气体中,该成螨的耗氧量反倒低于其在正常大气中的耗氧量。在0%、8%、16%、32%、64%CO2与0.05mg/LPH3混合气体中该成螨对PH3的吸收量分别为1.11±0.92、1.79±0.56、5.14±1.13、7.60±1.80、8.08±0.85μg/hr’g,在同一CO2浓度条件下试螨对PH3的吸收量在高浓度PH3(0.45mg/L)中明显大于在低浓度PH3(0.05mg/L)中,但PH3吸收量的增加倍数远远低于PH3浓度的增加倍数。PH3对该螨过氧化氢酶的抑制体内酶高于离体酶,细胞色素c氧化酶受PH3抑制则相反。被PH3抑制的过氧化氢酶和细胞色素C氧化酶活性恢复时间分别为二周和一周。本文还对PH3的可能杀螨机理及CO2在此过程中的作用进行了讨论 相似文献
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心房钠尿肽对大鼠主动脉弓压力感受器活动的影响 总被引:2,自引:0,他引:2
在隔离灌流的大鼠主动脉弓-主动脉神经标本上,观察了心房肽Ⅲ(atriopeptinⅢ,APⅢ)对主动脉弓压力感受器活动的影响。主动脉弓内压保持13.3kPa条件下向灌流液中加入APⅢ(2.0μg/ml)后,主动脉神经传入放电较对照值增加64±27%(P<0.001);升降灌流压时,主动脉神经传入放电随之增减,饱和压(SP)由22.5±0.5降至21.3±0.5kPa(P<0.05),工作范围(OR)由12.1±0.4降至10.6±0.4kPa(P<0.05),阈压(TP)无明显变化;压力感受器机能曲线向左上方移位,曲线的最大积分值(PIV)由508±66%增至730±52%(P<0.05),曲线最大斜率(PS)由55.6±7.5增至93.2±6.8%·kPa-1(P<0.05);冲洗掉APⅢ后,主动脉神经传入放电基本恢复至对照水平.主动脉弓内压保持在13.3kPa条件下,向灌流液中加入硝普钠(NP,1.0μg/ml),主动脉神经传入放电虽有所减少,但无统计学意义(P>0.05);升降灌流压时,TP,SP,OR均无明显变化,对压力感受器机能曲线及PS也无显著影响,而PIV由667±78降至502±74%(P< 相似文献
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血小板生长因子(PDGF—BB)刺激体外培养兔肺动脉平滑肌细?… 总被引:2,自引:0,他引:2
应用细胞培养、^3H-TdR和^3H-Leucine掺入方法,观察血小板生长因子BB对体外培养兔肺动脉平滑肌细胞DNA和蛋白质合成的影响。结果表明:(1)当PDGF-BB浓度为10ng/ml时,^3H-TdR掺入值已较对照组显著提高(6262.5±412.9vs833.5±124.0,P〈0.05);当PDGF-BB浓度为20ng/ml时,^3H-Leucine掺入值亦较对照线显著增高(10212 相似文献
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乌龟胚胎及仔龟的卵黄代谢 总被引:5,自引:0,他引:5
乌龟卵、卵黄、剩余卵黄和仔龟的含水量分别为68.87%、61.47%、60.75%和76.43%;干重分别为2.2915±0.2101g,1.3071±0.2913g,0.2681±0.0241g和1.2305±0.2994g;脂肪含量分别为18.03%、31.76%、32.10%和19.30%。卵黄、剩余卵黄和仔龟的热值分别为6.71kcal/gDM,6.76kcal/gDM和4.10kcal/ 相似文献
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雄螨(Malemite)是否存在第二若螨期(Deutongmph-period)各种资料说法不一,且国内尚未见这方面的详细报道。笔者于1994~1997年以截形叶螨TetranychustruncatusEhara为试验材料,对此进行了初步研究,现将结果报告如下。1螨种与试验条件螨种于1994年8月在延边大学农学院附近的菜豆上采集,经鉴定后在室内25℃、14小时/天光照条件下饲养,供试验用。本试验在自动控制温度(±0.5℃)和光照长度(±0.01小时/天)的小室内进行。小室大小为1.7m×1.4… 相似文献
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Profiles of SARS-CoV-2 RNA and Antibodies in Inpatients with COVID-19 not Related with Clinical Manifestation: A Single Centre Study 下载免费PDF全文
Zhao Li Gao Ruqin Lu Roujian Wang Huijuan Deng Yao Niu Peihua Jiang Fachun Huang Baoying Liang Jiwei Jia Jing Zhang Feng Wang Wenling Wu Guizhen Tan Wenjie 《中国病毒学》2021,36(5):1088-1092
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RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
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JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献
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Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme
responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare
the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show
that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by
distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of
demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least
one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of
the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable
potent antiproliferative synthetic drugs. 相似文献
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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases 相似文献