共查询到20条相似文献,搜索用时 46 毫秒
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福尔马林固定云南鲴的DNA提取及其细胞色素b基因序列分析 总被引:12,自引:0,他引:12
福尔马林固定云南鲴的DNA提取及其细胞色素b基因序列分析DNAEXTRACTEDFROMFORMALIN-FIXEDXenocyprisyunnanensisANDSEQUENCEANALYSISOFITSCYTOCHROMEBGENE关键词福尔马林... 相似文献
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一种从鱼类卵巢制备地高辛标记mtDNA探针的简易方法 总被引:5,自引:1,他引:4
一种从鱼类卵巢制备地高辛标记mtDNA探针的简易方法*ASIMPLEMETHODFORGETTINGDIGOXIGENINLABELINGPROBLEOFmtDNAFROMOVARYOFFISH关键词鱼类,线粒体DNA,地高辛标记KeywordsFi... 相似文献
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陈清轩 《基因组蛋白质组与生物信息学报(英文版)》1995,(1)
DetectionandAnalysisofanEstrous-associatedOviductalGlycoproteinDNAFragmentfromPrimatesbyPCR¥CHENQing-xuan(陈清轩);ClaytonE.Walto... 相似文献
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以嗜热脂肪芽孢杆菌为材料,通过PolyminP沉淀、硫酸铵分级及DEAE纤维素、磷酸纤维素、Blue-Sepharose、FPLCMonoQ、FPLC Superose12等柱层析,得到了部分纯化DNA解链蛋白BstH2。BstH2具有受DNA促进的ATP酶活力,没类型的核酸对BstH2的ATP酶活力的促进作用没。BstH2在55℃有最高ATP酶活力。这种活力受大肠杆菌单链DNA结合蛋白的抑制及随 相似文献
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鸡减蛋综合征病毒(EDSV—76)基因组E1区结构特点分析 总被引:1,自引:0,他引:1
EDSV-76病毒中国株AA-2经常规方法提取其病毒DNA后,建立了限制性内切酶PstI水解片段的全基因文库。对其中PstI-G片段和PstI-A片段的正反链进行序列测定,获得EDSV E1区(0-8.8m.u)的核苷酸序列。经分析,EDSV E1区具有与其他腺病毒E1区类似的结构。以大于60个氨基酸残基为标准,EDSV E1区共有7个开放读码框架(ORF),其中R1、R2、ElbsT和E1b1T 相似文献
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以Epstein-Barr病毒(EBV)DNA聚合酶为抗原,建立了简便、快速、敏感和特异的鼻咽癌诊断方法。构建原表达载体pRSET-DNA聚合酶及其亚克隆PRSET-A1和BL21(DE3)中表达的产物,经Western-blot检测其抗原性并用于检测鼻咽癌(nasopharyngeal carcinoma,NPC)病人血清中的抗体。在DPC病人血清中存在抗EB病毒DNA聚合酶的IgG抗体,并证明 相似文献
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嗜热脂肪芽孢杆菌DNA解链蛋白1的纯化及性质 总被引:1,自引:1,他引:0
以嗜热脂肪芽孢杆菌为材料,通过PolyminP沉淀,硫酸铵分级及Phenyl-Sepharose,DEAE纤维素,磷酸纤维素,FPLC MonoQ,FPLC Superose12等柱层析,得到部分纯化的DNA解链蛋白1。BstH1具有依赖DNA和Mg^2+的ATP酶活力,不同类型的核酸对BstH1的ATP酶活力的促进作用不同。 相似文献
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Michael Hesse 《Plant Systematics and Evolution》1980,134(3-4):229-267
Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout. 相似文献
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Surveillance of Class Ⅰ Newcastle Disease Virus at Live Bird Markets and Commercial Poultry Farms in Eastern China Reveals the Epidemic Characteristics 下载免费PDF全文
Xiaolong Lu Xiaoquan Wang Tiansong Zhan Yifan Sun Xin Wang Naiqing Xu Tianxing Liao Yu Chen Min Gu Shunlin Hu Xiaowen Liu Xiufan Liu 《中国病毒学》2021,36(4):818-822
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正Dear Editor,Parainfluenza virus 5 (PIV5), known as canine parainfluenza virus in the veterinary field, is a negative-sense,nonsegmented, single-stranded RNA virus belonging to the Paramyxoviridae family (Chen 2018). The virus was first reported in primary monkey kidney cells in 1954 (Hsiung1972), then it has been frequently discovered in various 相似文献
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Altaf Hussain Tiantian Wu Hui Li Linjin Fan Kai Li Li Gao Yongqiang Wang Yulong Gao Changjun Liu Hongyu Cui Qing Pan Yanping Zhang Asim Aslam Khan Muti-Ur-Rehman Muhammad Munir Salman Latif Butt Xiaomei Wang Xiaole Qi 《中国病毒学》2019,34(1):102-105
<正>Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However, there is limited genome information of IBDV 相似文献
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Yanling Yang Yuening Cheng Nan Li Shipeng Cheng Li Guo Yucheng Zhou Haiwei Zhang Xinyuan Zhang Linzhu Ren 《中国病毒学》2018,33(6):561-564
正Dear Editor,Mink circovirus (MiCV), which is clustered in the genus Circovirus of the family Circoviridae, was first described in minks from farms in Dalian, China in 2013 (Lian et al.2014). The complete single-stranded circular genome of the virus is 1,753 nucleotides long and contains two major open reading frames (ORFs), designated ORF1 (Rep gene)and ORF2 (Cap gene)(Lian et al. 2014; Ge et al. 2018).Sequence analysis has shown that MiCV is most closely 相似文献
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Madina Mahesutihan Weinan Zheng Liang Cui Yun Li Pengtao Jiao Wenxian Yang Wei Liu Jing Li Wenhui Fan Limin Yang Wenjun Liu Lei Sun 《中国病毒学》2018,33(5):440-448
Cyclophilin A (CypA) is a peptidyl-prolyl cis/trans isomerase that interacts with the matrix protein (M1) of influenza A virus (IAV) and restricts virus replication by regulating the ubiquitin–proteasome-mediated degradation of M1. However,the mechanism by which CypA regulates M1 ubiquitination remains unknown. In this study, we reported that E3 ubiquitin ligase AIP4 promoted K48-linked ubiquitination of M1 at K102 and K104, and accelerated ubiquitin–proteasome-mediated degradation of M1. The recombinant IAV with mutant M1 (K102 R/K104 R) could not be rescued, suggesting that the ubiquitination of M1 at K102/K104 was essential for IAV replication. Furthermore, CypA inhibited AIP4-mediated M1 ubiquitination by impairing the interaction between AIP4 and M1. More importantly, both the mutations of M1 (K102 R/K104 R) and CypA inhibited the nuclear export of M1, indicating that CypA regulates the cellular localization of M1 via inhibition of AIP4-mediated M1 ubiquitination at K102 and K104, which results in the reduced replication of IAV.Collectively, our findings reveal a novel ubiquitination-based mechanism by which CypA regulates the replication of IAV. 相似文献
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Zhiyun Yin Fuli Ren Min Zhou Shengyao Chen Yali Deng Sijing Hu Fei Deng Shu Shen Junming Shi 《中国病毒学》2022,37(6):946-949