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1.
为了综合评价常用杀螨剂对荔枝叶螨Oligonychus litchii的室内毒力,在室内分别采用Potter喷雾法、浸叶碟法和浸叶法测定了5种常用杀螨剂对荔枝叶螨成螨、卵和若螨的毒力,并测定了5种杀螨剂对荔枝叶螨的田间防效。室内毒力结果表明:哒螨灵、阿维菌素对荔枝叶螨成螨和若螨有较高的毒力,联苯肼酯对成螨和若螨的毒力较低,而螺螨酯和乙螨唑对成螨基本无活性,对若螨有一定的毒力;5种杀螨剂对卵的毒力大小依次为乙螨唑螺螨酯哒螨灵阿维菌素联苯肼酯。田间防效试验中,15%哒螨灵乳油和1.8%阿维菌素乳油对荔枝叶螨的总体防效较好,药后1 d防效高于70%,药后10 d防效达100%,药后20 d防效仍在90%以上,速效性和持效性均较好;240 g/L螺螨酯悬浮剂和110 g/L乙螨唑悬浮剂对荔枝叶螨药后3 d防效均低于70%,而药后20 d的防效高于90%,说明两药剂速效性较差但持效性较好;43%联苯肼酯悬浮剂药后1、3 d防效低于阿维菌素和哒螨灵,高于螺螨酯和乙螨唑,药后15 d防效开始下降。在田间防治荔枝叶螨时,若荔枝叶螨发生早期、成螨较少时建议施用螺螨酯或乙螨唑,而荔枝叶螨种群中成螨、若螨较多时推荐施用阿维菌素、哒螨灵或联苯肼酯。  相似文献   

2.
【目的】为明确不同杀螨剂对朱砂叶螨Tetranychuscinnabarinus不同发育阶段的生物活性。【方法】采用浸渍法分别测定了10种杀螨剂对朱砂叶螨成螨、卵和若螨的毒力。【结果】丁氟螨酯、阿维菌素和联苯肼酯对成螨、卵和若螨的活性均较高;乙螨唑和螺螨酯对卵和若螨活性较高,但对成螨活性明显偏低;甲氰菊酯对成螨和若螨的活性优于卵;唑螨酯、哒螨灵和三唑锡对3种螨态也均具有毒杀作用,但毒力偏低。同一种杀螨剂对若螨的活性均高于成螨和卵,乙螨唑、螺螨酯、联苯肼酯、唑螨酯和炔螨特对卵的活性高于成螨,丁氟螨酯、阿维菌素、甲氰菊酯、哒螨灵和三唑锡对成螨的活性高于卵。【结论】不同杀螨剂对朱砂叶螨不同发育阶段毒力存在较大差异,田间用药防治时应根据害螨发生情况和发生阶段,选择适合的防治药剂。  相似文献   

3.
姜黄素类化合物对朱砂叶螨的生物活性   总被引:6,自引:1,他引:5  
张永强  丁伟  赵志模 《昆虫学报》2007,50(12):1304-1308
分别用玻片浸渍法和叶片浸渍法测定了从姜黄中分离的姜黄素(curcumin,CCM)、去甲氧基姜黄素(demethoxycurcumin,DMC)和双去甲氧基姜黄素(bisdemethoxycurcumin,BDMC) 3种天然姜黄素类化合物对朱砂叶螨Tetranychus cinnabarinus Boisduval的成螨、若螨、卵的触杀活性以及对成螨产卵的抑制作用。结果表明:3种化合物对朱砂叶螨的生物活性大小依次为BDMC>DMC>CCM。3种化合物对朱砂叶螨成螨触杀活性最高者为BDMC,处理24 h和48 h其LC50分别为1.18和0.51 mg/mL。对若螨触杀活性的大体趋势与对成螨的相同,其中处理48 h,BDMC对若螨的LC50最小,为2.48 mg/mL。3种化合物对朱砂叶螨卵的触杀毒力也同样表现为BDMC>DMC>CCM。3种单体化合物都表现出一定的对朱砂叶螨雌成螨的产卵抑制作用。对姜黄素类化合物构效关系的初步研究,明确了甲氧基在姜黄素模板上对杀螨活性的贡献,对于开发具经济价值的叶螨类杀螨剂或者筛选先导化合物模板,具有一定的参考价值。  相似文献   

4.
【目的】评价联苯肼酯对智利小植绥螨Phytoseiulus persimilis的安全性,以及该药剂与捕食性天敌智利小植绥螨联合使用的效果。【方法】采用室内生物测定与田间试验的方法测定了联苯肼酯对智利小植绥螨成螨和若螨致死率与繁殖能力的影响,以及二者联合使用对茄子上二斑叶螨的控制效果。【结果】结果表明143 mg/L联苯肼酯对智利小植绥螨成螨和若螨的存活和生殖能力均无显著影响,处理后96 h对若螨的最高致死率为2.30%,成螨为2.04%;处理组8 d的平均产卵量为15.08粒/雌,与对照平均产卵量15.45粒/雌无显著差异;处理组所产卵的平均孵化率为98.63%,与对照组平均孵化率98.13%无显著差异;联苯肼酯悬浮剂在143 mg/L浓度下对二斑叶螨的控制效果表现为速效性高于单独使用智利小植绥螨,但持效性低于智利小植绥螨,防效在第22天时开始下降。二者联合使用表现出较好的速效性与持效性,处理后第2天防效达97.35%,第18天时达100%。【结论】联苯肼酯对智利小植绥螨具有极高的安全性,该药剂与智利小植绥螨联合使用对二斑叶螨具有良好的控制效果。  相似文献   

5.
通过施用不同类型的杀螨剂对苹果山楂叶螨Tetranychus viennensis和天敌塔六点蓟马Scolothrips takahashii的影响进行分析,结果表明,15%哒螨灵EC(0.4 mL/L)、73%炔螨特EC(0.5 mI/L)表现出较好的速效性和持效性,但是对塔六点蓟马有一定的杀伤作用,240 g/L螺螨酯SC(0.2 mL/L)、5%唑螨酯SC(0.4 mL/L)对山楂叶螨相对防效较好.对塔六点蓟马影响较小,500 s/L四螨嗪SC(0.2 mL/L)速效性较差,但对天敌安全,20%甲氟菊酯EC(0.5 mL/L)对山楂叶螨防治效果差,且对天敌杀伤率始终较高,从而可能造成山楂叶螨的爆发.复配药剂10%阿维·四螨SC(0.67 mL/L)综合了单剂的优点,15.6%阿维·丁醚EC(0.5 mL/L)、6%阿维·哒EC(0.5 mL/L)相对单剂对天敌杀伤率有所降低,1.8%阿维·甲氰EC(0.83 mL/L)则主要表现出单剂甲氰菊酯相同的特点.  相似文献   

6.
在室温26℃±1℃、湿度60%~80%、光照14 h条件下,测定了天然植物活性成分双去甲氧基姜黄素对重要植食性害螨朱砂叶螨各螨态的触杀和熏蒸活性,对幼螨、若螨、成螨的驱避活性,以及对雌成螨的产卵抑制活性.采用玻片浸渍法测得双去甲氧基姜黄素对朱砂叶螨雌成螨48 h的LCso为0.433 mg·mL-1.在0.883 mg·mL-1(LC70)的浓度下,双去甲氧基姜黄素对朱砂叶螨不同螨态触杀活性的大小依次为幼螨>若螨>成螨>卵,其中对幼螨24h和48 h的校正死亡率分别为60.0%和83.3%;对朱砂叶螨各螨态的熏蒸作用不明显,024h和48 h的校正死亡率均小于3%;对幼螨、若螨以及成螨均表现出较强的驱避作用,其中对幼螨的效果最好,不同处理时间的驱避率均在85%以上,其次是若螨,对成螨的驱避性相对较差,72 h的驱避率仅为47.8%.同时对雌成螨有明显的产卵抑制作用,处理后120 h产卵抑制率达到89.3%.表明双去甲氧基姜黄素对朱砂叶螨的主要作用方式为触杀、驱避和产卵抑制作用.  相似文献   

7.
测定了木鳖子提取物对朱砂叶螨的触杀活性。用甲醇、氯仿、石油醚3种不同极性的溶剂提取,石油醚提取率最高为30.79%,且对朱砂叶螨成螨和卵的触杀活性均高于其他两种溶剂的提取物,24 h校正死亡率分别为77.52%和72.04%。用甲醇对石油醚提取物萃取,发现甲醇萃取物活性明显高于石油醚部分,对成螨和卵处理24 h后的校正死亡率分别为89.60%和74.65%,产卵抑制率为62.74%,驱避率为58.23%。柱层析对甲醇萃取物进行分离得到10种组分,组分5活性最高,浓度为2 mg/mL时,24 h校正死亡率为86.15%。用薄层层析和气相色谱质谱联用仪分别检测组分5纯度和主成分,初步确定活性成分为α-菠菜甾醇。  相似文献   

8.
高温冲击对山楂叶螨的影响   总被引:3,自引:0,他引:3  
李定旭  张晓宁  杨玉玲  朱华伟 《生态学报》2010,30(16):4437-4444
为探索高温冲击对山楂叶螨的影响,在室内采用叶碟饲养的方法,将山楂叶螨不同螨态暴露于33-42℃高温下1-6h,然后在温度(25±1)℃、相对湿度(60±7)%、光周期16h∶8h(L∶D)下测定其寿命、产卵量和孵化率。结果表明,高温冲击对山楂叶螨的影响主要表现在对其产卵量和孵化率的影响,而对成螨的寿命无明显影响;影响的程度取决于高温的强度、持续时间以及处理的螨态。卵经历33-42℃的高温处理1-6h,其孵化率无明显变化,但在随后的发育过程中,幼若螨的发育历期在39℃和42℃6h处理中显著延长,发育至成螨后其产卵量分别增加34.50%和37.41%;幼螨经历39℃和42℃的高温处理6h后发育至成螨时产卵量比对照高出27.02%和35.83%;静止期第二若螨经历39℃和42℃的高温处理,其发育成的雌雄螨的交配和受精能力无明显影响;初羽化雌成螨经历39℃和42℃的高温处理6h后,产卵量不受影响,但卵的孵化率降低了7.01%-11.36%。  相似文献   

9.
【目的】丁氟螨酯是一种新型酰基乙腈类非内吸性杀螨剂,对害螨的各个螨态都有很高活性,具有较高的应用价值。本文评价了丁氟螨酯对二斑叶螨生长发育的影响,以期为合理用药和二斑叶螨的综合防治提供理论依据。【方法】采用浸叶法测定丁氟螨酯对二斑叶螨成螨与卵的致死中浓度、雌成螨产卵量、各螨态存活率以及各发育历期的影响。【结果】经丁氟螨酯处理后,二斑叶螨日均产卵量和总产卵量下降,各螨态发育历期延长;LC70、LC50、LC30剂量处理二斑叶螨雌成螨后,每雌日均产卵量为(2.09±0.17)、(3.02±0.22)、(3.39±0.13)粒,每雌总产卵量为(29.29±2.31)、(42.32±3.01)、(47.41±1.77)粒,与空白对照差异显著(P<0.05,df=3);不同剂量处理二斑叶螨卵后,各螨态发育历期的延长程度不同,但成功孵化的卵能完成发育历期,LC 70剂量延长卵历期至(4.45±0.07)d、前若螨历期至(2.75±0.04)d、后若螨历期至(2.61±0.05)d和总历期至(12.53±0.18)d,LC 50剂量延长前若螨历期至(2.52±0.08)d、后若螨历期至(2.67±0.09)d和总历期至(12.22±0.18)d,LC 30剂量延长前若螨历期至(2.45±0.06)d和总历期至(11.53±0.08)d,与空白对照差异显著(P<0.05,df=3)。【结论】丁氟螨酯能对二斑叶螨的生长发育产生明显影响,降低二斑叶螨的产卵能力,抑制卵孵化,延长发育历期,从而降低二斑叶螨种群的发育速率,对该螨的种群控制有积极意义。  相似文献   

10.
【目的】为解决当前苹果园二斑叶螨Tetranychus urticae抗药性高、防治效果差、用药量大的问题,评价当前登记药剂及果园正在使用的药剂对二斑叶螨的防治效果。【方法】采用室内生测的方法比较了10种登记药剂,9种果园正在使用的药剂对二斑叶螨的毒力效果。【结果】选用的登记药剂中,80%的药剂对二斑叶螨效果差,甚至失去控制作用,防治效果低于66.67%,仅双甲脒、阿维菌素48 h防效在92.68%以上;果园常用药剂螺螨酯、虫酰肼、噻虫啉对二斑叶螨均表现出很好的防治效果,48 h防效达到84.09%-100%。【结论】目前80%以上的登记单剂药剂对二斑叶螨失去了防控效果,这是果园二斑叶螨防效差的主要原因,常用药剂螺螨酯、虫酰肼、噻虫啉等对二斑叶螨防效好。建议增加高效药剂登记或更换防治药剂,保证对二斑叶螨的防效,降低果园用药量,提高果园用药系统的精准性。二斑叶螨防治不能仅依赖化学药剂,要充分发挥果园生态系统的自然控害功能,延缓抗药性发展。  相似文献   

11.
In this study the reproductive capacity of a laboratory-selected spirodiclofen resistant strain was investigated after treatment with spirodiclofen. Firstly, females were exposed to different concentrations of spirodiclofen (200 and 1,000 mg/l) during 6, 12 or 24 h. In contrast to the susceptible parental strain, the fecundity and fertility of resistant mites was not affected by treatment with these concentrations after any time of exposure tested. Secondly, pre-treatment of the resistant females with the synergists PBO or DEF could increase the inhibitory effect of spirodiclofen on reproduction, demonstrating the possible involvement of monooxygenases and esterases in metabolic detoxification of the acaricide. Because spirodiclofen interferes with lipid biosynthesis, total lipid content was measured in female adults. There were no significant differences between treated and non-treated female adults, both in the susceptible and resistant strain. However, the total lipid content in the resistant females was significantly higher than in susceptible females. Our data shows that the detection of spirodiclofen resistance should not be limited to mortality bioassays with eggs or larvae, but should be combined with inhibitory studies on female fertility and fecundity.  相似文献   

12.
Laboratory bioassays were conducted to evaluate the effects of spirodiclofen on life history and life-table parameters of two-spotted spider mite (Tetranychus urticae Koch) females treated at pre-ovipositional period with a series of acaricide concentrations starting with the concentration discriminative for eggs and immatures i.e. the lowest concentration that causes 100% mortality of those stages. After a 24 h exposure, the proportion of females that survived treatments was 0.71 (6 mg/l), 0.51 (12 mg/l), 0.41 (24 mg/l), 0.30 (48 mg/l) and 0.25 (96 mg/l). At the end of the trial, the survival rate of females treated with the lowest concentration was significantly lower than the survival rate of untreated females but it remained above that of females treated with higher concentrations. Total fecundity/fertility significantly decreased as concentrations of spirodiclofen increased. Viable eggs were laid by females treated with 6, 12 and 24 mg/l, and total fertility was reduced by 42, 84 and 97%, respectively. Compared with control, the gross fecundity/fertility of the treated females was significantly reduced throughout the trial, except in females treated with 6 mg/l. All concentrations caused a significant reduction in the net fecundity/fertility throughout the trial. The females treated with 12 mg/l had significantly reduced net reproductive rate (R 0 = 6.45), compared to females treated with 6 mg/l (R 0 = 23.35) and to untreated females (R 0 = 28.92); there was no significant difference between the last two treatments. The intrinsic rate of increase (r m ) and finite rate of increase (λ) were significantly reduced in treated females (r m = 0.141, λ = 1.156 and r m = 0.214, λ = 1.232; 12 and 6 mg/l, respectively), compared to control (r m = 0.251, λ = 1.276). The reduction was significantly greater in females treated with the highest concentration. As a result of the lowered r m , the doubling time in treated females was significantly extended. Sublethal effects of spirodiclofen and its impact on T. urticae management are discussed.  相似文献   

13.
The toxicity of selected commercial formulations of neem on Tetranychus urticae Koch (Acari: Tetranychidae) and two predatory mites Euseius alatus De Leon and Phytoseiulus macropilis (Banks) was studied. Topical toxicity was tested with the commercial formulations (Natuneem, Neemseto and Callneem) and extract of neem's seeds at concentration 1%, compared to the standard acaricide abamectin at concentration of 0.3 ml/L and the control treatment (distilled water). Based on the best performance against T. urticae through topical contact, the formulation Neemseto was selected to be evaluated using different concentrations against eggs, and residual and repellent effects on adults of the mites. Egg treatment consisted of dipping eggs into Neemseto dilutions and control treatment for five seconds. In addition, residual and repellent effects of Neemseto for adult mites consisted of using leaf discs dipped into the dilutions for five seconds. The toxicity of Neemseto on eggs and adults was greater for T. urticae compared to the toxicity observed for the predatory mites. Neemseto was repellent for T. urticae and E. alatus when tested at the concentrations of 0.25, 0.50 and 1.0%, and did not affect P. macropilis. Neemseto using all concentrations, while for the predatory mites significant reduction of mite fecundity was only observed at the largest concentrations reduced the fecundity of T. urticae significantly. So Neemseto, among tested neem formulations, performed better against the twospotted spider mite and exhibited relatively low impact against the predatory mites studied.  相似文献   

14.
Laboratory bioassays were conducted to evaluate the effects of spiromesifen on gross fecundity, gross fertility, net fertility and population growth of two-spotted spider mite (Tetranychus urticae Koch) after treatments with four acaricide concentrations: 180 mg/l, i.e. maximum recommended concentration for use in glasshouses against spider mites, 18, 1.8, and 0.18 mg/l, i.e. concentration discriminative for eggs and immatures in preliminary studies which produced 100% mortality of these stages. Quiescent female deutonymphs were treated in the first assay, and young pre-ovipositing females in the second and third, in which exposure lasted 6 h and 20 h, respectively. In the first assay, the 180, 18, and 1.8 mg/l concentrations significantly reduced gross fecundity (61–85%), gross fertility (64–87%) and net fertility (85–94%) of the surviving females. In the second one, only the highest concentration achieved a significant statistical reduction in gross fecundity (52%), gross fertility (67%) and net fertility (84%). In the third assay, fecundity and fertility reduction under the two highest concentrations was 98–99% and 93–98%, whereas it was 50–74% under the 1.8 mg/l concentration, and statistically different from control values. In all three trials, treatments with 180, 18, and 1.8 mg/l concentrations significantly reduced the instantaneous rate of increase. In the third assay, treatments with the two highest concentrations caused population decline. Sublethal activity of the 0.18 mg/l concentration was not found in any assay to be statistically significant. Sublethal effects of spiromesifen and its impact on T. urticae management are discussed.  相似文献   

15.
Northern fowl mites were monitored on a caged-layer operation in southern California for 22 mo. Three experienced observers underestimated actual numbers of mites in the vent region approximately 80% of the time. Errors were higher for heavy infestations. Observer estimates were highly correlated with each other (r > 0.89, P < 0.01) and with mite numbers estimated by vent feather removal (r > 0.82, P < 0.01). Mites on hens varied between houses and over time. Molting consistently reduced mite numbers, but did not eliminate then in a flock. Long-term monitoring of individual sentinel hens demonstrated that some hens would support high numbers of mites for several months or more. Use of a new sequential hen sampling plan required approximately 1 min per hen, if mite numbers were estimated. At this site, treatment decisions often could be reached in < 20 min per house. Mite scores (index of estimated mites per hen) were well correlated with percentage of hens infested in both test houses. In a chronically infested house, prevalence of mites on eggs averaged 8.5%, with a range of 0-55%. Applications of tetrachlorvinphos-dichlorvos by the producer appeared to be based on mites on > about 20% of eggs. The chemical was marginal for controlling mites on hens (25% reduction in percentage of hens infested), but effectively reduced mites on eggs (95% fewer mites on eggs at 1 wk and 90% at 2 wk). When data were grouped by mite index score on hens, there was a strong relationship (r2 = 0.83, P < 0.01) between mite prevalence on eggs and the scores of the hens which laid them. Sampling 100 eggs evenly spaced in a house required < 7 min, and adult mites were easily seen. Sampling mites on eggs appears to be useful to localize at least high-level infestations, and egg-based sampling for mites merits further investigation.  相似文献   

16.
Both prey density and developmental stage of pests and natural enemies are known to influence the effectiveness of biological control. However, little is known about the interaction between prey density and population structure on predation and fecundity of generalist predatory mites. Here, we evaluated the functional response (number of prey eaten by predator in relation to prey density) of adult females and nymphs of the generalist predatory mite Euseius concordis to densities of different developmental stages of the cassava green mite Mononychellus tanajoa, as well as the fecundity of adult females of the predator. We further assessed the instantaneous rate of increase, based on fecundity and mortality, of E. concordis fed on eggs, immatures and adults of M. tanajoa. Overall, nymphs and adults of E. concordis feeding on eggs, immatures and females of M. tanajoa had a type III functional response curve suggesting that the predator increased prey consumption rate as prey density increased. Both nymphs and adult females of the predator consumed more eggs than immatures of M. tanajoa from the density of 20 items per leaf disc onwards, revealing an interaction between prey density and developmental stage in the predatory activity of E. concordis. In addition, population growth rate was higher when the predator fed on eggs and immatures in comparison with females. Altogether our results suggest that E. concordis may be a good candidate for the biological control of M. tanajoa populations. However, the efficiency of E. concordis as a biological control agent of M. tanajoa is contingent on prey density and population structure.  相似文献   

17.
The false spider mite, Brevipalpus californicus (Banks), is a major citrus pest in Texas. This phytophagous mite causes damage to fruit, leaves and stems of citrus, and is also a vector of citrus leprosis virus. Galendromus helveolus (Chant) is one of the most prevalent predacious mite species found on Texas citrus. The predation potential of G. helveolus on different stages of B. californicus was evaluated in the laboratory. An individual immature G. helveolus mite consumed an average of 30.7 eggs, 53.6 larvae, or 22.7 nymphs of B. californicus before developing to an adult. An individual adult female G. helveolus consumed an average of 164.8 eggs, 369.6 larvae, or 80.9 nymphs of B. californicus. Both immature and adult G. helveolus never fed on adult stage of B. californicus and never completed development. The development times of the immature stages of G. helveolus were 4.5, 4.1 and 4.6 days when fed on eggs, larvae and nymphs of B. californicus, respectively. When G. helveolus fed on the larval stage of B. californicus, the adults had the longest longevity (18.5 days) and the highest fecundity (14.0 eggs/female).  相似文献   

18.
在实验室条件下研究了辣椒碱对烟粉虱的生物活性及生长发育和生殖力的影响.结果表明:辣椒碱对烟粉虱各虫态均有杀虫活性,且随辣椒碱浓度的增大活性增强;辣椒碱浸叶法/浸虫法处理对烟粉虱成虫、卵和若虫的毒力(LC50分别为3525.79、1603.29和1526.27mg·L-1))均高于内吸法处理(LC50分别为5360.04、2359.19和5897.43 mg.L-1),两种处理方法下辣椒碱对烟粉虱卵的毒力均明显高于对成虫的毒力;2000和4000 mg·L-1浓度辣椒碱对烟粉虱成虫具有明显的拒食作用,烟粉虱在该两种浓度辣椒碱处理过的棉花叶上取食24 h的蜜露分泌量均显著低于取食非处理棉花叶后的蜜露分泌量;辣椒碱各试验浓度对烟粉虱成虫均表现出较强的产卵忌避作用,当辣椒碱处理浓度达4000 mg·L-1时,对烟粉虱的选择性产卵忌避率和非选择性产卵忌避率分别高达94.2%和83.0%.500、1000和2000 mg·L-1辣椒碱浸渍处理对烟粉虱存活卵和若虫的发育历期、成虫羽化率均没有影响,但辣椒碱4000mg·L-1处理明显延长烟粉虱若虫发育历期、降低成虫羽化率.不同浓度辣椒碱处理均明显抑制烟粉虱生殖力,且这种抑制作用随辣椒碱浓度的增大而增强.  相似文献   

19.
The daily rate of oviposition, fecundity, survival and adult longevity ofOrius albidipennis (Reuter) (Hemiptera: Anthocoridae) were studied in the laboratory in Israel. These parameters were compared on three arthropod prey species: the two-spotted spider mite,Tetranychus urticae Koch; the onion thrips,Thrips tabaci Lindeman; and eggs of the almond moth,Ephestia cautella Walker. The fecundity and survival on the thrips diet (217.2 eggs/female and 98.7%, respectively) and on moth eggs (184.1 eggs/female and 84.6%, respectively) were significantly higher than on the spider mite diet (110.9 eggs/female and 40.4%, respectively). Female longevity was significantly higher onEphestia eggs (63.0 days) than on thrips (45.1 days) and mites (35.1 days). There were no significant differences in male longevity among the three diets (57.5, 64.1 and 54.5 days, respectively).  相似文献   

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