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1.
近几年来,酶传感器、免疫传感器及微生物传感器等发展较为成熟,而DNA生物传感器的研究相对较少。文章从核酸杂交的原理出发介绍了DNA生物传感器的工作原理,举例说明了电化学、光学和声学等几种典型的DNA生物传感器,指出了其固有的优缺点,肯定了DNA传感器发展前景。  相似文献   

2.
核酸探针传感器的构建   总被引:8,自引:0,他引:8       下载免费PDF全文
依据石英振子的晶体谐振频率是其表面沉积物的函数,建立了简便、特异、敏感性达100pg,并能对受检样品相对定量的核酸杂交检测新方法,即压电晶体式核酸探针传感器,该方法的主要特点是以快速、敏感的频变信息作为核酸杂交的显示系统.  相似文献   

3.
DNA生物传感器及其研究进展   总被引:10,自引:0,他引:10  
就DNA生物传感器的工作原理,分类、DNA探针的固化方法,以及电化学DNA生物传感器、光学DNA生物传感器及压电DNA生物传感器的研究进展、优缺点和发展趋势加以介绍。  相似文献   

4.
DNA生物传感器在环境污染监测中的应用   总被引:10,自引:0,他引:10       下载免费PDF全文
基于生物催化和免疫原理的生物传感器在环境领域中获得了广泛应用.近年来,随着分子生物学和生物技术的发展,人们开发了以核酸探针为识别元件,基于核酸相互作用原理的DNA生物传感器.该传感器可用于受感染微生物的核酸序列分析、优先控制污染物的检测以及污染物与DNA之间相互作用的研究,在环境污染监测中具有潜在的巨大应用前景.简要介绍了核酸杂交生物传感器的基本原理及其在环境微生物和优先控制污染物(priority pollutant)检测中的应用研究进展.  相似文献   

5.
光纤DNA传感器   总被引:2,自引:0,他引:2  
光纤DNA传感器是DNA生物传感器中很有潜力、极具发展前途的一种,具有重要的研究价值和诱人的应用前景。该技术灵敏度高,分析操作简便、快速,为分子生物研究提供了一种新方法,在基础医学、临床医学和预防医学各个领域具有十分广泛的应用前景。本文对光纤DNA传感器的原理,DNA探针在光纤表而的固定化,光纤DNA传感器的类型等方面的研究进展作了评述。  相似文献   

6.
本从杂交指示剂的特性和选用出发,分别讨论了基因电化学传感器和光化学传感器的设计原理及应用。  相似文献   

7.
纳米粒子标记DNA探针在电化学DNA生物传感器中的应用   总被引:3,自引:0,他引:3  
高梅 《生物磁学》2006,6(1):16-19
介绍了纳米电化学DNA生物传感器的基本概念和分类,并介绍了用于DNA标记的纳米粒子的六种类型及其三大检测方法,在此基础上对纳米电化学DNA生物传感器在基因检测、疾病诊断、DNA检测等方面的最新进展进行了综述与讨论.  相似文献   

8.
DNA生物传感器研究进展   总被引:1,自引:0,他引:1  
本根据作用机理不同将DNA生物传感器分为DNA光化学传感器,DNA电化学传感器和压电晶体传感器,并就几种方面的研究进展进行了综述。  相似文献   

9.
消失波生物传感器及其在DNA与免疫分析中的应用   总被引:1,自引:0,他引:1  
消失波光纤生物传感器是近年来发展很快的一项的分析技术。它现在已成为分了生物学领域的热门技术。本文叙述消失波生物传感器的识别元件,换能装置以及检测研究系统的研究进展。着重讨论消失波技术在DNA检测与免疫检测中的应用。并对这些技术的应用价值做出评价。  相似文献   

10.
基于引物链延伸反应的基因传感器的研究   总被引:1,自引:0,他引:1  
依据晶体谐振频率是其表面沉积物的函数和模板-引物杂交后引物链延伸的酶促反应原理,构建了引物链延伸反应性基因传感器技术.该技术的主要特点是以快速、敏感的频变信息作为基因杂交与引物链延伸的显示系统.研究结果提示,本项技术可用于核酸同源性分析、微量DNA检测、DNA整合性的确定,也可用于目的基因的分离、特定条件下的基因突变分析及推断某基因在其基因组中的位置.  相似文献   

11.
RNA—RNA原位杂交实验条件探讨   总被引:1,自引:0,他引:1  
干月波  郑树 《生物技术》1991,1(6):15-21
将Vigilin和qroal(Ⅰ)cDNA亚克隆到pGEM3Z和pGEM4Z载体,体外转录合成35s标记的cRNA探针。经RNA凝胶电泳,Southern Northern,杂交检查探针长度,杂交特性和特异性,通过系列实验探讨了RNA-RNA原位杂交实验中固定、杂交前处理、杂交温度,探针量、探针长度,洗脱严格性和RNA酶处理等对杂交结果的影响,建立了简化的RNA-RNA原位杂交方法。  相似文献   

12.
  总被引:1,自引:0,他引:1  
Based on the fact that the resonant frequency of a piezoelectric crystal is the function of its surface deposit, and that the primer extends after it hybridizes with the template, the primer extension gene sensor technique was developed. The prominent feature of the technique is that fast and sensitive frequency signals are used as the monitoring system of gene hybridization and primer strand extension. Results show that this technique may be used in homologous analysis of nucleic acid, trace DNA detection, and determining the integration of DNA. It may also be used for isolation of target gene, gene mutation analysis, and predicting the location of a gene in its genome.  相似文献   

13.
Hybridization of nucleic acids immobilized on solid supports   总被引:252,自引:0,他引:252  
  相似文献   

14.
    
The direct labeling of nucleic acid probes, with horseradish peroxidase (HRP) may be used in many membrane hybridization applications, including Southern blots, Northern blots, colony and plaque screening, PCR products detection/identification. This article describes the preparation method, which involves the labeling of a single-stranded nucleic acid probe with a positively charged HRP-parabenzoquinonepolyethyleneimine complex (labeling reagent). The associated hybridization and posthybridization protocols are relatively simple, which makes probes labeled directly with HRP particularly suitable for large scale screening, where tens or hundreds of blots are processed weekly.  相似文献   

15.
    
The principle objectives when creating a robust DNA diagnostic assay system are sensitivity, specificity and minimal read-time. To meet these ends, depending on the specifically defined test, various aspects of molecular hybridization methodology must be optimized. In particular, among other things, attention has focused on (i) formulating highly specific probes; (ii) devising sensitive nonisotopic detection systems, (iii) minimizing the extent of preparing clinical samples for assaying, (iv) amplifying the target sequence to augment sensitivity and (v) enhancing hybridization kinetics to speed up the reaction period. In this article, some recent studies that are directed to the development of nucleic acid hybridization systems for clinical diagnosis of microorganisms are considered.  相似文献   

16.
核酸生物传感器及其研究进展   总被引:4,自引:0,他引:4       下载免费PDF全文
核酸生物传感器在涉及分子生物学的研究领域具有重要意义.为适应分子生物学及其相关学科的发展需要,其研究正成为90年代生物传感技术研究热点.文章对核酸生物传感器的工作原理、分类、研究现状以及发展趋势作了较详细的介绍.  相似文献   

17.
    
Thiolated pyrrolidinyl peptide nucleic acids (HS-PNAs) bearing d-prolyl-2-aminocyclopentanecarboxylic acid (ACPC) backbones with different lengths and types of thiol modifiers were synthesized and then characterized by MALDI–TOF mass spectrometry. These HS-PNAs were immobilized on gold-coated glass by self-assembled monolayer (SAM) formation via S atom linkage for the detection of DNA hybridization using surface plasmon resonance (SPR). The amount and the stability of the immobilized HS-PNAs, as well as the effects of spacer and blocking thiol on DNA hybridization efficiency, were determined. SPR results indicated that the hybridization efficiency was enhanced when the distance between the PNA portion and the thiol terminal was increased and/or when blocking thiol was used following the HS-PNA immobilization. The immobilized HS-PNA could discriminate between fully complementary DNA from one or two base mismatched DNA with a relatively high degree of mismatch discrimination (>45%) in PBS buffer at 25 °C. The lowest DNA concentration at which reliable discrimination between fully complementary and single mismatched DNA could still occur was at about 0.2 μM, which is equivalent to 10 pmol of DNA. This research demonstrates that using these novel thiolated PNAs in combination with the SPR technique offers a direct, rapid and non-label based method that could potentially be applied for the analysis of genomic or PCR-amplified DNA in the future.  相似文献   

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