叶面肥对玉米籽粒中矿物元素的影响分析

Fe、Zn、Se等矿物元素在动植物以及人体的生长和发育过程中具有重要作用,为了提高玉米籽粒中Fe、Zn、Se的含量,对叶面喷施Fe、Zn、Se肥及其不同组合,探讨其对玉米成熟籽粒中的Fe、Zn、Se以及其它矿物元素含量的影响。使用不同浓度的硫酸亚铁(Fe肥)、硫酸锌(Zn肥)和亚硒酸钠(Se肥)及其不同组合对京科糯2010(JK2010)进行了叶面喷施处理。叶面喷施不同浓度的Fe肥、Zn肥和Se肥均能显著提高JK2010籽粒中Fe、Zn、Se元素的含量;Fe与Zn能够相互促进吸收,Se能够促进Zn的吸收,Fe与Zn对Se的积累有抑制作用;叶面喷施Fe、Zn、Se肥及其组合对玉米籽粒中Ca元素的积累有促进作用;不同的Fe、Zn、Se肥组合方式对籽粒中Cu的积累有不同的影响;总体上看叶面喷施Fe、Zn、Se肥及其组合对玉米籽粒中Mn、Mg、P等元素的积累影响不大。不同的叶面喷施组合处理中,Fe肥、Zn肥和Se肥三者配合使用能极显著的提高籽粒中Fe、Zn、Se元素的含量,为Fe、Zn、Se肥的生产应用提供新的策略,同时也发现Fe、Zn、Se肥的使用对籽粒中几种不同的矿物元素有不同的影响。     

小麦叶面喷施微肥对籽粒中矿物元素的影响分析

在人体和动植物的生长发育过程中,铁(Fe)、锌(Zn)、硒(Se)3种微量元素起着重要的作用。为了提高小麦籽粒中Fe、Zn、Se元素的含量及进一步分析叶面喷施Fe、Zn、Se肥及其组合对籽粒中Fe、Zn、Se以及其他矿物元素含量的影响,并寻求生产上最适宜的叶面喷施组合,采用不同浓度的硫酸亚铁(Fe肥)、硫酸锌(Zn肥)和亚硒酸钠(Se肥)及其组合对小麦品种北京0045(BJ0045)进行叶面喷施处理。结果表明,叶面喷施Fe、Zn、Se肥均能显著提高BJ0045籽粒中Fe、Zn、Se元素的含量;Fe与Se能够相互促进吸收,Zn能够促进Se与Fe的吸收,Se对Zn有抑制作用;不同的叶面喷施组合处理中,Fe/Zn/Se三者混合肥的使用能显著提高籽粒中Fe、Zn、Se元素的含量,同时发现不同的叶面喷施组合的施用对籽粒中Ca、Mg、Cu、Mn、P矿物元素的吸收也有不同的影响。     

氮磷肥对茶树锌硒等中微量元素吸收与分配的影响

锌（Zn）和硒（Se）及其他中微量元素（铝Al，钙Ca，铁Fe，铜Cu，锰Mn）是茶叶品质的重要指标，但茶树吸收Zn、Se能力及氮（N）磷（P）肥影响中微量元素吸收与分配的过程尚不清楚。以红壤丘陵区福鼎大白茶树为研究对象，开展Zn+Se、Zn+Se+N、Zn+Se+P、Zn+Se+N+P和对照共5种处理3次重复随机化区组试验，处理第3年春季分茶叶、成熟叶、吸收根、运输根和储藏根采集植物样品，测定其元素含量。结果表明，茶树地上和地下器官Zn和Se及其他中微量元素对N、P、Zn、Se添加的响应具分异性。与对照相比，茶树地上和地下器官Zn和Se含量均显著增加，与Zn+Se相比，施N和/或P肥仅显著提高茶叶和成熟叶Se含量（P<0.05）；与对照相比，施肥处理均显著提高吸收根和运输根Al、Fe含量以及储藏根Cu含量；运输根Mn含量表现为Zn+Se+N、Zn+Se+P、Zn+Se+N+P显著高于对照，储藏根Mn含量为Zn+Se+N+P显著高于其他处理；茶树各器官Ca含量对施肥处理无显著响应。此外，茶叶和成熟叶的Zn含量与吸收根显著正相关，而Se含量则与储藏根显著正相关。茶树具有吸收和积累Zn和Se的能力，而施N、P肥有助于提高茶叶Se含量，研究结果为红壤丘陵区培育高品质锌硒茶及营建生态高值茶园提供了依据。     

Cu、Zn对水蕹菜生长的影响及Se的缓解作用

为了解Cu、Zn对水蕹菜生长的影响以及Se缓解Cu、Zn胁迫的效果,本试验以大叶水蕹菜和柳叶水蕹菜为材料,通过营养液栽培,研究了Cu、Zn处理下水蕹菜产量、生理指标和Cu、Zn含量的变化以及Se对Cu、Zn胁迫下水蕹菜生长的影响。结果表明:低浓度Cu、Zn促进水蕹菜生长;高浓度Cu、Zn抑制其生长,表现为根系活力、抗氧化酶活性和净光合速率(Pn)下降,MDA含量增加。Cu、Zn浓度分别为4.00和8.00 mg·L~(-1)时,水蕹菜产量显著下降。Cu对水蕹菜的毒害高于Zn,柳叶水蕹菜的抗氧化能力高于大叶水蕹菜。水蕹菜中Cu、Zn含量随处理浓度的增加而增加。SOD、POD、CAT和脯氨酸在缓解低浓度Cu、Zn毒害中具有协同作用。高浓度Cu、Zn处理时,水蕹菜抗氧化酶活性受到抑制,则以植物络合素(PCs)和脯氨酸的抗氧化作用为主。Se能提高水蕹菜、抗氧化酶活性以及PCs和脯氨酸含量,增强水蕹菜对Cu、Zn的抗性,提高根系活力和Pn,降低MDA的积累,促进水蕹菜生长。当水蕹菜受到轻度Cu、Zn毒害时,5.00 mg·L~(-1)的Se处理缓解效果较好。Se浓度过高会加剧Cu、Zn对水蕹菜的毒害。     

籼稻和粳稻品种糙米矿质营养对开放式空气CO_2浓度升高的响应

基于FACE(free-air CO_2enrichment)试验平台,以籼稻IIY084与粳稻WYJ23为试验对象,连续两年(2013、2014)研究糙米产量和矿质元素(Fe、Mn、Cu、Zn、Ni、Se)含量对CO_2浓度升高的响应。结果表明,CO_2浓度升高条件下,IIY084与WYJ23籽粒和糙米产量增加,其中,糙米两年平均增产34.3%和16.3%。CO_2浓度升高连续两年降低IIY084糙米中Fe、Mn、Cu、Zn、Ni、Se含量,其两年平均分别降低了10.4%、13.4%、15.5%、11.7%、25.7%、3.2%,而WYJ23糙米中矿质营养含量未出现一致的下降现象。其中,2014年糙米Mn、Cu、Zn和Ni含量对CO_2浓度升高的负响应达到显著水平(P0.05)。此外,糙米Fe(P0.1,2014)、Mn(P0.01,2013和2014)、Cu(P0.1,2014)、Zn(P0.01,2013和2014)、Ni(P0.01,2014)、Se(P0.05,2013; P0.01,2014)含量在水稻品种间存在显著性差异。CO_2浓度升高不同程度地增加糙米中矿质元素积累量,6种元素两年平均增加2.1%～29.2%(IIY084)和12.8%～19.3%(WYJ23)。可见,CO_2浓度升高条件下,IIY084糙米中矿质元素含量较WYJ23有更明显的下降趋势,导致食用IIY084较WYJ23有更大的Fe、Mn、Cu、Zn、Ni、Se缺乏风险。因而,在确保粮食安全而进行增产品种选育时,应该优先选择矿质营养品质较高的水稻品种。     

马麝全血和被毛矿物元素测定

检测了马麝主要树叶饲料和健康马麝全血、被毛中6种微量元素(Cu、Mn、Fe、Zn、Mo、Se)及2种常量元素(Ca、Mg)的含量,并与当地健康山羊的全血和被毛值进行了比较分析。结果表明,马麝的10种树叶饲料为低Se饲料,被毛中Cu、Mn、Zn、Ca、Mg含量明显高于全血水平,而Fe则反之。马麝全血和毛样中的各元素分布比较集中,离散度均很小。     

环青海湖地区土壤、牧草及牦牛血液中矿物质元素动态变化研究

2005年3月(冬春季草场)和2005年7月(夏季草场),分别对环青海湖地区放牧牦牛的全血、牧草和土壤中的Zn、Ca、Cu、Fe、K、Na、S、P、Cl、F、I、Se 12种矿物元素和Ca、Cu、K、Fe、Na、P、Se 7种矿物元素的营养状况进行了测定.结果 显示,除夏季牦牛全血Se和S有显著增加外,其它矿物质含量均显著降低,提示夏季草场由于牦牛生长发育迅速其矿物质元素的缺乏更为严重,在生产中更应进行有关矿物质元素的补饲,进一步提高其生产性能.     

蛇岛蝮蛇的微量元素研究

经 ICP-AES 测定,在蛇岛蝮蛇体内含有 Zn、Cu,Fe、Mn、Mo、Cr、Co、Se、V、Sn、Si、Li、Te.Al、As,Ge 和 Pb 等多种微量元素,但各器官之间存在显著差异.Zn 几乎存在于蛇体内的所有组织,而且含量极为丰富.蛇体内的 Cr、Sn、Te、Al 含量较高,但 Cu、Mn、Mo.V 等含量甚微.胆汁,血清具有高 Te、Cr、Pb、As,Li、低 Fe、Zn 的特点,而肝、脾、血液不仅 Fe 贮量丰富,且 Sn 含量也高.     

磁化微咸水灌溉对欧美杨I-107微量元素和碳氮磷养分特征的影响

为探讨盐分环境下,磁化作用对土壤和植株养分特征的影响,以欧美杨I-107为试材,采用磁化和非磁化微咸水灌溉处理,通过对土壤和植物组织中微量元素和碳氮磷含量的测定,以揭示土壤-植物的养分供求关系。研究发现:(1)微咸水灌溉处理中叶片和根系Fe含量均下降;叶片Zn、Mn和Cu含量提高,根系Mn和Zn含量降低、Cu含量提高。叶片和根系中总碳含量均提高,全氮和全磷含量均降低;叶片C/N下降,根系C/N和C/P则提高。(2)磁化微咸水灌溉处理叶片和根系中Fe、Zn、Cu含量均提高,Mn含量降低;叶片C、P及C/N提高,N含量下降;根系C、C/N和C/P含量提高。(3)微咸水灌溉土壤中Fe、Mn、Zn和Cu微量元素全量均降低,有效态含量均提高;OC和N、C/P和N/P下降,全磷和C/N提高。(4)磁化微咸水灌溉中土壤Fe、Mn和Zn全量提高,Cu降低;Fe、Mn、Zn和Cu有效态含量提高;OC和N、C/P和N/P提高。可见,磁化作用有利于调节植株对微量元素的吸收和分配,提高土壤的固氮能力以及对植株的碳供应能力。此外,盐分环境下,植物体通过提高元素Fe含量以及C/N比值维持光合作用的正常进行,以满足植株正常生长发育的需要。     

外源NO对缺镁胁迫下玉米幼苗生长和离子平衡的影响

研究了在缺镁胁迫下,外源NO对缺镁玉米幼苗生长、根系活力和离子含量的影响。结果表明,缺镁胁迫使玉米幼苗株高、根长和干鲜重下降,根系活力降低,N元素在地上部和根部分配失调,新叶和老叶中Mg2＋、Cu2＋、Fe3＋、Mn2＋等离子含量下降,Ca2＋、K＋、Zn2＋等离子含量上升。根中Mg2＋离子含量下降,Ca2＋、K＋、Zn2＋、Cu2＋、Fe3＋、Mn2＋等离子含量上升。用100μmol·L-1一氧化氮供体硝普钠（SNP）处理后,玉米幼苗株高、根长、干重和鲜重均提高,根系活力增强,改善了N代谢,新叶中Ca2＋、K＋和Zn2＋等离子含量下降,Mg2＋、Cu2＋、Fe3＋和Mn2＋等离子含量提高,老叶中Mg2＋、Ca2＋、K＋和Zn2＋等离子含量下降,Cu2＋、Fe3＋和Mn2＋等离子含量提高,根中Mg2＋、Ca2＋、K＋、Cu2＋、Zn2＋、Fe3＋和Mn2＋离子含量均下降。实验结果表明,NO保护玉米幼苗免受缺镁胁迫的影响。     

Selenium, zinc, and copper concentrations in the blood and milk of lactating women

The aim of the study was to determine Se, Zn, and Cu concentrations in blood plasma and milk of lactating women from central Poland who were in different stages of lactation and to investigate the relationship between the content of trace elements in mothers’ blood and concentrations of microelements in their milk. Se and Zn concentrations in blood plasma of mothers were the lowest and Cu was the highest on the first 4 d of lactation (colostrum, n=43) and were found to be 34.9±11.8 μg/L, 0.51±0.13 mg/L, and 1.70±0.55 mg/L, respectively. The highest plasma level of Se and Zn and the lowest content of Cu could be observed between d 10 and 30 of lactation (mature milk, n=41), and were found to be 54.3±14.6 μg/L for Se (p<0.001), 0.76±0.20 mg/L for Zn (p<0.001), and 1.03±0.30 mg/L (p<0.001) for Cu. The results of Se, Zn, and Cu determination in breast milk samples demonstrate a pattern of decline in their concentration with advancing stages of lactation. We found out that Se, Zn, and Cu concentrations were the highest in colostrum (n=43) and amounted to 24.8±10.1 μg/L, 8.2±2.8 mg/L, and 0.45±0.11 mg/L, respectively. The content of all determined microelements declined significantly during the time of lactation. Statistically significant linear correlation was found between concentrations of Zn in blood plasma and milk in the first stage of lactation. Weak but statistically significant linear correlations were also found between plasma Se content in plasma and in transitional and mature milk of breast-feeding women.     

Prophylactic iron supplementation in pregnant women in Norway

In the present study 67 non-anaemic women were randomly allocated to either 100 mg or 15 mg iron daily at about the 10. week of pregnancy. At about week 18, 30 and 36 of pregnancy, as well as 6 weeks after delivery, hemoglobin and the serum concentrations of ferritin, vitamin B12, folates, Zn, Cu and Se were monitored. Dietary allowances of other minerals and vitamins are also increased in pregnancy, and the 15 mg iron tablet was enriched with Zn (10 mg), Cu (2 mg), Se (50 microg), vitamin B12 (3 microg), and folate (0.1 mg). Neither ferritin, nor Cu, Zn or Se concentrations differed statistically significantly between the treatment groups during pregnancy. Ferritin and Zn appeared to decrease approximately parallel to the hemodilution, whereas Cu concentrations increased from a non-pregnant reference mean of 18 micromol Cu/L to a maximum mean of nearly 33 micromol Cu/L during pregnancy. Se decreased concomitantly to about 1.0 micromol Se/L. Serum folate (around 15 micromol/L) was essentially unaffected by pregnancy in the group given multivitamin/mineral supplementation, whereas the mean concentration fell below 10 micromol/L in the group supplemented with 100 mg iron daily. Our results indicate that supplementation of 15 mg Fe daily during pregnancy results in a small reduction of hemoglobin. It is suggested that additional supplementation with folate might be of importance to maintain the serum folate concentration during pregnancy.     

Copper,selenium, and zinc concentrations in human milk during the first three weeks of lactation

Human milk samples were analyzed by neutron activation analysis (NAA) for three essential trace elements (Cu, Se, and Zn). Mothers' milk samples were collected from 25 lactating mothers from the Central Hospital, Tripoli, Libya. The average concentration level of Cu, Se, and Zn declined from 0.84 +/- 0.06 mg/L, 104 +/- 9.46 microg/L, and 16.1 +/- 2.67 mg/L at d 0 to 0.39 +/- 0.045 mg/L, 41.8 +/- 6.66 microg/L, and 4.95 +/- 1.3 mg/L, respectively, at d 20 of lactation. Copper and Zn levels in the Libyan mothers' milk were in agreement with reported levels from other countries, whereas Se was at a higher level. The Cu daily intake level is slightly higher than the RDA (Recommended Daily Allowance) value. Selenium and Zn have higher intake levels than the RDA values but within the tolerable upper intake levels.     

Interaction of trace elements in a longitudinal study of human milk from full-term and preterm mothers

Concentrations of 8 trace elements (Fe, Cu, Zn, Se, Br, Pb, Rb, and Sr) at different lactation time were measured by the PIXE multi-elemental technique. Time dependence and interelement correlations were studied. A total of 200 milk samples from 32 lactating mothers were supplied from 2 to 120 d after delivery of 26 full-term and 6 preterm infants. All elements showed a lognormal frequency-distribution. The Fe, Cu, Zn, and Se contents in preterm milk were found to be somewhat different with respect to full-term milk. Cu, Zn, Se, Br, Pb, and Rb concentrations declined with lactation time, both in pre- and full-term samples. Sr and Fe contents did not show any change with time. Detailed analysis of data by partial correlation and multiple regression methods was performed. No substantial differences between preterm and full-term samples were found in the results of partial correlation analysis. Cu and Zn were found to be correlated with lactation time, whereas the measured time dependence for the other elements has to be attributed to the effect of the existing interelement correlation. All the measured elements appeared to be correlated with at least one other element. In particular, Se was inversely correlated with Zn and directly with Cu. The zinc and copper contents in milk can therefore depend on the variation in the mother selenium intake.     

Serum and tissue trace elements in patients with breast cancer in Taiwan

The objective of this study was to compare levels of four elements (zinc, copper, selenium, and iron) in the serum and tissue of 68 breast tumor patients (benign and malignant), from a teaching hospital in central Taiwan. Samples of normal tissue (5 cm away from tumor) were also taken from patients with malignant tumors. Only serum was taken from the 25 healthy persons in the control group. Results showed that Zn, Cu, Se, Fe, Cu/Zn, Cu/Se, and Cu/Fe were present in different amounts in the serum of each of the three groups. Zn and Se levels were lower in the serum of the two tumor groups compared to the control group. In tissue samples, Zn, Cu, Se, and Fe concentrations were different in each of the three groups. The malignant tissue had the highest levels of all four elements. In advanced-stage malignant tumors, levels of Cu and the ratios of Cu/Fe and Cu/Zn (in both serum and tissue) were highest. The ratios of serum Cu/Zn, Cu/Fe, and Cu/Se were also higher in malignant patients. The cutoff value of serum Cu/Zn was 1.2 (sensitivity and specificity were both 100%). The Cu/Zn ratio was highest in the advanced stages of cancer and was a better diagnostic tool for breast cancer than Cu/Se and Cu/Fe. The authors suggest that change of trace elements in serum and tissue might be useful and significant as biomarkers involving the initial plastic process.     

人工饲养与野生川金丝猴体毛10种微量元素的含量及比较

测定了秦岭人工饲养(10只)和野生(14只)川金丝猴体毛中的10种微量元素含量。锌、铁、铜、钙、镁5种元素采用火焰原子吸收法;锰、铬、铅采用石墨炉原子吸收法;铝采用等离子光谱;硒经硝解后采用原子吸收法测定。结果表明,铬、锰、镁、铅、锌和硒的含量,人工猴极显著或显著高于野生猴;铁含量,人工猴极显著低于野生猴;钙、铜和铝的含量,人工猴与野生猴无显著差异。以人类毛发10种微量元素的正常范围为参照,人工猴铅、铬、锰与锌4种含量均显著超出正常范围的上限,属于严重超量。这可能与金丝猴饲养过程中添加营养制剂有关。     

Serum and tissue levels of six trace elements and copper/zinc ratio in patients with cervical cancer and uterine myoma

The aim of this study is to investigate the relationship between trace elements and the incidence of cervical cancer. Tissue and serum levels of six elements (Cu, Zn, Fe, Mn, Ca, and Se) and the Cu/Zn ratio in 40 cases of patients with cervical cancer, 30 cases of uterine myoma, and 50 healthy subjects were measured by atomic absorption spectrophotometry; the selenium content was determined by atomic fluorescence spectrometry. The results showed that the tissue contents of Zn, Se, and Ca were significantly lower and the Cu and Fe concentrations and Cu/Zn ratio were significantly higher in cervical cancer tissue than that for paired nonlesion tissue (p<0.02 and p<0.001, respectively). The serum levels of Zn, Se, Ca, and Fe were lower and Cu and Mn levels and Cu/Zn ratio were higher in patients with cervical cancer than in healthy subjects (p<0.01 and p<0.001, respectively) and in the uterine myoma group compared with healthy subjects (p< 0.05–0.001). There are no significant differences in the contents of six elements and the Cu/Zn ratio between uterine myoma tissue and paired nonlesion tissue. The results showed also that the Fe level and Cu/Zn ratio were significantly higher and the Zn and Se levels were significantly lower in cervical cancer tissue than in uterine myoma tissue (p<0.01 and p<0.001, respectively). The serum Cu level and Cu/Zn ratio were significantly higher in the cervical cancer group than the uterine myoma group (p<0.01). Data were also analyzed using multivarate logistic regression. After adjustment for age, occupation, life habit, and other covariates for the development of cervical cancer, the odds ratios were 22.64 (95% confidence interval [CI]: 5.64–90.88, p=0.001) for Cu, 0.11 (95% CI: 0.034–0.373; p=0.005) for Zn, and 0.60 (95% CI: 0.36–0.99, p=0.01) for Se. Thus, the serum and tissue levels of Cu increase and the deficiency of Zn and Se may be risk factors for the development of cervical cancer.     

Effect of magnesium deficiency on enterocyte Ca, Fe, Cu, Zn, Mn and Se content

In previous studies based on indirect procedures, we reported that Mg deficit increased the bioavailability of a number of elements such as calcium, zinc, iron, copper, manganese and decreased selenium absorption. The present study was designed to verify these findings by direct methods. We investigated the effect of dietary magnesium deficiency on enterocyte Ca, Fe, Zn, Cu, Mn and Se concentrations. Male Wistar rats were fed a Mg-deficient diet (129 mg Mg/kg food) for 70 days. Whole enterocytes from the upper jejunum were isolated and Ca, Fe, Zn, Cu, Mn and Se were determined. The results were compared with findings in a control group that was pair-fed with an identical diet except that it covered this species's nutritional requirements for Mg (480 mg Mg/kg food). The Mg-deficient diet significantly increased enterocyte content of Ca, Fe, Zn, Cu and Mn; however, we found no significant changes in the Se content of these cells. These data support the results obtained by indirect methods.     

Mineral content in the wild onion species and their nutritive value

The accumulation of 11 microelements (Fe, Zn, Cu, Co, Mn, Ni, Pb, Cd, Sr, Cr, and Se) by 7 wild onion species of the genus Allium was studied. A. flavescens Bess possesses the higher accumulating capability; it accumulates five of eleven microelements (Cr, Ni, Cu, Zn, and Se) with biological accumulation coefficient (BAC) exceeding 1 and also contains high levels of Fe, Co, and Mn. These features enable this species to be the most promising for the nutrition adjustment of human commons with above-mentioned microelements primarily with Cr, which content in 100 g onion leaves achieves 84% of commons. A. fistulosum L., A. odorum L., and broad-leaved A. nutans form specifically accumulate Cu, Zn, and Se. A. montanum Schmidt is characterized by the accumulation of Zn. A. angulosum and A. schoenoprasum are xharacterized by the accumulation of Se. The broad-leaved forms of A. schoenoprasum L., A. nutans L., and A. odorum L. compared with their narrow-leaved forms accumulate more amounts of Zn and Cu.     

Essential trace elements in milk and blood serum of lactating donkeys as affected by lactation stage and dietary supplementation with trace elements

The aim of this trial was to study the concentration of zinc (Zn), iron (Fe), copper (Cu), manganese (Mn), selenium (Se), cobalt (Co) and iodine (I) in milk and blood serum of lactating donkeys, taking into account the effects of lactation stage and dietary supplementation with trace elements. During a 3-month period, 16 clinically healthy lactating donkeys (Martina-Franca-derived population), randomly divided into two homogeneous groups (control (CTL) and trace elements (TE)), were used to provide milk and blood samples at 2-week intervals. Donkeys in both groups had continuous access to meadow hay and were fed 2.5 kg of mixed feed daily, divided into two meals. The mixed feed for the TE group had the same ingredients as the CTL, but was supplemented with a commercial premix providing 163 mg Zn, 185 mg Fe, 36 mg Cu, 216 mg Mn, 0.67 mg Se, 2.78 mg Co and 3.20 mg I/kg mixed feed. The concentrations of Zn, Fe, Cu, Mn, Se, Co and I were measured in feeds, milk and blood serum by inductively coupled plasma-MS. Data were processed by ANOVA for repeated measures. The milk concentrations of all the investigated elements were not significantly affected by the dietary supplementation with TE. Serum concentrations of Zn, Fe, Cu Mn and Se were not affected by dietary treatment, but TE-supplemented donkeys showed significantly higher concentrations of serum Co (1.34 v. 0.69 μg/l) and I (24.42 v. 21.43 μg/l) than unsupplemented donkeys. The effect of lactation stage was significant for all the investigated elements in milk and blood serum, except for serum manganese. A clear negative trend during lactation was observed for milk Cu and Se concentrations (−38%), whereas that of Mn tended to increase. The serum Cu concentration was generally constant and that of Co tended to increase. If compared with data reported in the literature for human milk, donkey milk showed similarities for Zn, Mn, Co and I. Furthermore, this study indicated that, in the current experimental conditions, the mineral profile of donkey milk was not dependent on dietary TE supply.