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目的应用无创遥测技术观察运输应激对Beagle犬部分生理指标的影响。方法 16只Beagle犬随机分成两组(每组8只),即对照组和运输应激组,并利用大动物无创生理信号遥测技术,分别监测清醒自由活动状态下对照组和运输应激组应激4 h后Beagle犬的心电图、活动、皮肤温度和呼吸参数的变化。结果 Beagle犬心率、RR间期、QT间期、活动、皮肤温度、呼吸均具有明显的昼夜节律变化(P〈0.01);与对照组比,运输应激后Beagle犬心率、活动度、呼吸频率、每分钟通气量和潮气量均显著增加(P〈0.01),RR间期、PR间期、皮肤温度均显著降低(P〈0.01),相关分析表明运输应激对Beagle犬心率、活动、皮肤温度和呼吸频率具有显著的相关性(P〈0.05,P〈0.01)。结论运输应激可引起Beagle犬生理学指标的明显改变,但除皮肤温度外,运输应激(4 h)对Beagle犬昼夜节律变化破坏不明显;利用无创遥测技术平台可建立理想的Beagle犬生物模型,可用于动物福利和药理毒理学评价研究。 相似文献
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1988年测定栽种于山东农业大学蔬菜标本园的生姜(Zingiber officinalis)品种“莱芜片姜”的某些生理指标和生化物质含量变化,得到如下结果,供参考:种姜在萌芽时,其淀粉及干物质含量迅速下降(图1,表1),至盛长期又逐渐回升,可溶性糖呈增加的趋势(表2);新姜(根茎)的淀粉及可溶性糖含量均随生长的进行而增加。这种变化与淀粉酶、转化酶活性变化相协 相似文献
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离子色谱仪是一种常用的实验设备,在测定离子物质的过程中起着关键的作用,被广泛运用于各种领域。文章深入探讨在水质检测和分析过程中使用离子色谱仪遇到的各种问题及其解决策略,以提升离子色谱仪的精度和稳定性,最大限度地利用其功能,为相关研究提供一定参考。 相似文献
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(一)血液中CO_2的运输形式血液中CO_2的运输有三种形式: 1.物理溶解的形式。 2.结合成HCO_3~-的形式,这种形式大部分在血浆中。 3.在红细胞中与Hb(还原型血红蛋白)结合成氨基甲酸血红蛋白(HbNHCOOH)的形式。后两种形式也可统称为化学结合形式。血液中以各种形式存在的CO_2的量大致如表1所示。 相似文献
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芒果生长发育过程中若干生理参数的变化 总被引:2,自引:0,他引:2
研究结果表明:芒果植(苗)株过氧化物酶同工酶基本酶谱共不10条,酶带面度2.67cm,同一器官生育期不同,酶谱、Rf值、酶活性和酶带分布均有明显差异;果实不同成熟阶段,呼吸跃变与乙烯产生相关密切。 相似文献
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<正>大熊猫(Ailuropoda melanoleuca)的健康关系到整个大熊猫种群的发展。对大熊猫血液生理生化指标的监测可以反映其健康状况及食物营养的合理性,受大熊猫种群数量及采样方式等因素的限制,关于大熊猫血液生理生化指标的报道较为有限,且动物的来源及采血状态均有很大的差异,结果也存在较大差异(Chen,1987;王强等,1998;李光汉等,1999;罗娌等,2017)。研究表明,体况(董全等,1991)、年龄(Mainka,1995;李才武等,2012;罗娌等,2017)、性别(Mainka,1995;罗娌等,2017)、地域(余昌萌等,2019)等是影 相似文献
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利用不同渗透势的培养液模拟土壤干旱条件,研究了玉米杂交种“中单2号”在此条件下的生长和生理的变化。实验表明,各指标对于渗透胁迫的变化敏感性顺序为叶片延伸速率、叶水势、脯氨酸含量>净光合速率>相对透性。玉米幼苗在渗透胁迫下具有一定的生理适应能力,表现在一定的渗透胁迫范围内随胁迫时间的延长,生长、生理变化有趋于缓和或恢复的趋势。 相似文献
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A protein unique to phosphorus stress observed in Dunaliella tertiolecta Butcher was studied in the context of phosphate-limited cell physiology and is a potential diagnostic indicator of phosphate deficiency in this alga. Cells were grown over a range of limited, steady-state growth rates and at maximum (replete) and zero (phosphate-starved) growth rates. The stress protein, absent in nutrient-replete cells, was produced under all steady-state phosphate-limited conditions and increased in abundance with increasing limitation (decreasing growth rate). Cellular carbon: phosphorus ratios and the maximum uptake rate of phosphate (Vm) increased with increasing limitation, whereas the ratio of chlorophyll a: carbon decreased. Alkaline phosphatase activity did not respond to limitation but was measurable in starved, stationary-phase cells. Fv/Fm, a measure of photochemical efficiency, was a nonlinear, saturating function of p, as commonly observed under N limitation. The maximum Fv/Fm of 0.64 was measured in nutrient-replete cells growing at μmax, and a value of zero was measured in stationary-phase starved cells. When physiological parameters were compared, the P-stress protein abundance and Fv/Fm were the most sensitive indicators of the level of deficiency. The stress protein was not produced under N- or Fe-limited conditions. It is of high molecular weight (>200) and is associated with internal cell membranes. The stress protein has several characteristics that make it a potential diagnostic indicator: it is 1) unique to phosphorus limitation (i.e. absent under all other conditions), 2) present under limiting as well as starved conditions, 3) sensitive to the level of limitation, and 4) observable without time-course incubation of live samples. 相似文献
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Near surface thermoclines form each day in the limnetic waters of Lake Titicaca (Peru-Bolivia) and thereby retain phytoplankton under extreme irradiances. This bright light exposure results in strongly depressed chlorophyll fluorescence and photosynthesis which both decay (bright light) and recover (dim light) by first order rate kinetics. During each afternoon the phytoplankton are redistributed by wind-induced mixing, and full recovery is accomplished soon after nightfall. In vivo fluorescence was measured over this diet cycle both with (Fb) and without (Fa 3-(3,4-dichlorophenyl)-1,1-dimethyl urea. Strongest bright light effects were on the parameter (Fa– Fa), a crude measure of operational photosystem II reaction centers (RC IIs). On dates of strong thermocline development, surface (FFa– Fa) was reduced to 5% or less of that for the mixed layer maximum. Fluorescence depression was greater in the lake than in Pyrex bottles incubated at fixed depths for 4 h. Ultraviolet light intensified the photoinhibitory response, but strong (Fb– Fa) depression could be induced by photosynthetically available radiation alone. In Lake Titicaca, photoinhibition apparently operates by reversible in-activation of RC IIs. It occurs in the natural water column and is not simply an artifact affixed bottle incubations. 相似文献
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During development (expansion), the cotton leaf passes through a stage in which it is highly susceptible to ozone. This period of susceptibility occurs after the maximum expansion rate but before complete expansion occurs. The period of maximum susceptibility corresponds to a minimum concentration of soluble sugars and free pool amino acids. Specific amino acids such as aspartate, serine, threonine, glutamate, asparagine, alanine, glycine, valine, isoleucine, leucine, histidine, and tryptophan tended to reach a minimum concentration at about the same time leaves were susceptible to ozone. Ozone exposure during the susceptible period is manifested by visible leaf flecking of the upper surface and a dramatic (up to 2 fold) increase in total free pool amino acids. Most individual amino acids tended to increase except for phenylalanine, alanine, phosphoserine, phosphoethanolamine, and ethanolamine. Soluble protein is decreased but not to the same extent that the free pool amino acids increase. Because there is ample evidence that ozone enters the leaf during nonsusceptible periods as well as susceptible periods, it is postulated that ozone damage results because of the depletion of soluble reserves (carbohydrates and amino acids). Perhaps repair of damage cannot occur. 相似文献
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Carlos Castillo-Chavez Simon A. Levin Fred Gould 《Evolution; international journal of organic evolution》1988,42(5):986-994
We develop a mathematical model to explore the evolution of habitat selection and physiological adaptation in a heterogeneous environment. The model assumes the following conditions: 1) a panmictic population of infinite size; 2) prereproductive individuals mobile enough to move between patches; 3) alleles at one locus code for absence or presence of adaptation to detrimental patches; 4) alleles at a second locus code for absence or presence of behavior(s) that cause avoidance of the detrimental patches; 5) additive effects of alleles controlling physiology and behavior; 6) frequency-independent fitness. Results of the model indicate that nontrivial, polymorphic equilibria do not exist. The pattern of genotypic fitnesses and the initial allelic frequencies can influence whether the population adapts by physiological or behavioral mechanisms, or by both. Linkage between the two loci can alter the outcome of evolution, given specified genotypic fitness values and initial allelic frequencies. 相似文献