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1.
对蕨类植物腊叶标本叶表皮的制片技术进行了一些改进,克服了传统叶表皮制片中易出现的不足之处,简化了整个制作过程.这种方法使得蕨类植物叶表皮显微制片技术更加完善,可以为从事植物形态分类学基础研究的工作者提供技术支持.  相似文献   

2.
易卷曲叶表皮制片技术(NaOCl法)的改进   总被引:14,自引:0,他引:14  
在利用NaOCl法制备用于光学显微镜观察的叶表皮过程中,一些科/属植物的叶表皮在100%乙醇脱水后遇二甲苯便发生卷曲,增加了叶表皮制片的难度,甚至无法进行。本文介绍一种简便易行的方法:在系列乙醇脱水后,加盖小块盖玻片,防止叶表皮脱水后卷曲,并使其保持平整,便于显微摄影。这种方法使得叶表皮显微制片技术更加完善。  相似文献   

3.
易卷曲叶表皮制片技术(NaOCl法)的改进   总被引:1,自引:0,他引:1  
洪亚平  陈之端 《植物学报》2002,19(6):746-748
在利用NaOCl法制备用于光学显微镜观察的叶表皮过程中,一些科/属植物的叶表皮在100%乙醇脱水后遇二甲苯便发生卷曲,增加了叶表皮制片的难度, 甚至无法进行。本文介绍一种简便易行的方法:在系列乙醇脱水后,加盖小块盖玻片,防止叶表皮脱水后卷曲,并使其保持平整,便于显微摄影。这种方法使得叶表皮显微制片技术更加完善。  相似文献   

4.
介绍一种禾本科植物鲜叶下表皮制片的快速方法,该方法也能适用于多数单子叶植物鲜叶和经FAA固定液固定的单子叶植物叶片的气孔观察。该方法制片能有效防止叶表皮脱水、透明后发生卷曲,使保持完整的结构,便于准确的显微观察。具操作简单、材料易得、可靠性和稳定性高等优点。  相似文献   

5.
章黎黎  李兴武 《西北植物学报》2013,33(11):2346-2350
为了简便而快速制作适用于光学显微镜观测的苹果属植物果实表皮标本,采用7种方法分别对小金海棠果实表皮进行整体制片后于光学显微镜下观察其形态特征,比较制片效果。结果表明:用离析刮片法、指甲油印迹法、刮片法和煮沸剥离刮片法制备的标本在光学显微镜下均呈现出清晰结构,其中煮沸剥离刮片法的制片效果最佳。因此,煮沸剥离刮片法最适用于苹果属植物果实表皮整体制片,该方法操作简便且效率高。  相似文献   

6.
简便有效的叶表皮离析方法——过氧化氢-醋酸法   总被引:1,自引:0,他引:1  
在长期叶表皮制片过程中发现,过氧化氢-醋酸离析法简便实用,对蕨类植物小型叶和大型叶、裸子植物鳞形叶、钻形叶、针形叶、条形或带形叶及被子植物宽大的叶片均能适用。尤其重要的是,过氧化氢-醋酸离析法不会破坏叶表皮细胞的结构,有利于精确观察叶表皮形态特征,是一种比较理想的叶表皮离析方法。  相似文献   

7.
生物标本石蜡包埋保存法其制作过程基本同显微镜永久玻片标本制片方法相似。新鲜的生物整体材料如植物柔软的果实、花、蕈类、小型哺乳动物、鱼类、两栖动物以及动物的局部器官和组织,经固定、脱水、石蜡渗透等步骤处理,可以制成干制本标。由  相似文献   

8.
汪矛  郑相如 《生命世界》1993,20(2):27-27
洋葱鳞叶表皮细胞是观察植物细胞的理想材料,它不仅取材容易,而且制片方法简单.长期以来,人们习惯用水装片法或加碘-碘化钾液染色,对其进行观察,收到了良好的效果.然而,上述方法制成的装片,染色较浅,材料不够平展,还易产生气泡,给观察带来一定不利.为此,我们对洋葱鳞叶表皮的永久装片进行了尝试.  相似文献   

9.
一种简便快速鲜叶表皮制片技术   总被引:4,自引:0,他引:4  
用透明胶带夹粘住适当大小的新鲜植物叶片,经轻轻挤刮,撕开两胶带,上、下表皮粘附在两胶带上,经NaOCl溶液处理3~5min,以离析粘附在表皮上的叶肉细胞,在流水下用软刷刷洗,凉干后,放在载玻片上盖上盖玻片,即可在显微镜下观察,摄影.该法简便、快速,更适用于叶片较小的植物叶表皮制片.  相似文献   

10.
九种海滨沙生植物解剖构造及其生态适应性研究   总被引:1,自引:0,他引:1       下载免费PDF全文
利用常规石蜡制片法,对生长于北戴河海滨沙地的9种沙生植物的营养器官的形态结构进行比较观察。结果表明:(1)叶结构出现各种旱生和盐生植物特征,如一些植物叶的栅栏组织发达,海绵组织退化;多数植物的叶片较小;多数植物支脉和侧脉多等;(2)轴器官具有发达的表皮和机械组织,有异常结构的出现。说明北戴河海滨沙滩生长的9种植物的营养器官存在一系列的旱生结构特征,这些结构在减少蒸腾作用,增强光合作用,储存水分,增强器官的机械强度方面具有积极的作用。  相似文献   

11.
Surgical technique for a permanent tracheostomy in Beagle dogs   总被引:3,自引:0,他引:3  
A surgical procedure to produce a permanent tracheostomy in dogs was developed. The procedure consisted of dissecting portions of the cartilaginous rings free from the underlying tracheal mucosa, cutting through the mucosa and suturing the mucosa to the skin. The procedure was performed in approximately 30 minutes and resulted in a permanent, maintenance-free, mucocutaneous stoma that also permitted breathing through the upper respiratory airways.  相似文献   

12.
Mesiodistal and buccolingual crown diameters were measured from dental casts representing the deciduous dentitions of 197 Aboriginal children from the Northern Territory of Australia. Double determination analysis indicated that the semi-automatic recording procedure used was reliable leading to observer errors of no practical significance. Tooth-size was greater in the male subjects but the sexual dimorphism was less marked than in the permanent teeth of the same subjects. The mandibular teeth were more uniform than maxillary with respect to buccolingual size relative to mesiodistal. Extremes of general tooth-size were more marked in the deciduous dentition than in the permanent as a consequence of the relatively large deciduous second molar which in Aboriginals approximates in size the permanent first molar of many other ethnic groups.  相似文献   

13.
Those unfortunate people who suffer from permanent partial facial paralysis have great difficulty finding surgeons who can offer corrective operations. Improving their function is a most delicate procedure. Great care must be exercised to avoid injuring nerves and muscles which are still operating, although in a greatly diminished state. The pathogenesis must be understood before attempting any corrective procedure. Adequate time must elapse from the moment of injury to surgical intervention, thus allowing for maximal nerve and muscle regeneration. This paper describes techniques that could improve facial movements. The most frequently used procedure is shortening of the levator and/or the zygomatic muscles that are partially atrophied. It must be understood that total reanimation is impossible as of this time.  相似文献   

14.
蓟马采集和玻片标本的制作   总被引:18,自引:0,他引:18  
张宏瑞  OKAJIMA Sh 《昆虫知识》2006,43(5):725-728
介绍蓟马标本的采集方法、常规鉴定用的临时性玻片和存档及分类用的永久性玻片标本的制作方法。标本的采集主要是拍打植物花朵、叶片及枯枝,玻片的制作重点介绍了制作存档和分类用的永久性玻片的5个步骤,即浸解脱色、洗涤、脱水、整姿封盖和干燥。  相似文献   

15.
doi: 10.1111/j.1741‐2358.2010.00419.x
Effect of accelerated ageing and surface sealing on the permanent deformation of auto‐polymerising soft linings Purpose: To compare the effects of different ageing methods on the permanent deformation of two permanent soft liners. Materials and methods: The materials selected were auto‐polymerising acrylic resin and silicone‐based reliners. Sealer coating was also evaluated. Sixty specimens of each reliner were manufactured (12.7 mm diameter and 19 mm length). Specimens were randomly distributed into 12 groups (n = 10) and submitted to one of the accelerated ageing processes. Permanent deformation tests were conducted with a mechanical device described within the American Dental Association specification number 18 with a compressive load of 750 gf applied for 30 s. All data were submitted for statistical analysis. Mann–Whitney test compared the effect of the surface sealer on each material and the permanent deformation of the materials in the same ageing group (p = 0.05). Kruskal–Wallis and Dunn tests compared all ageing groups of each material (p = 0.05). Results: The silicone‐based reliner presented a lower permanent deformation than the acrylic resin‐based reliner, regardless of the ageing procedure. The surface sealer coating was effective only for the thermocycled silicone group and the accelerated ageing processes affected only the permanent deformation of the acrylic resin‐based material. Conclusion: The silicone‐based reliner presented superior elastic properties and the thermocycling was more effective in ageing the materials.  相似文献   

16.
The increasing demand for sterilization as a permanent and effective form of birth control leads one to consider the various methods available to achieve this end. Tubal cauterization and excision by laparoscopy appears to be a safe, efficient method.The technique used is carefully reviewed step by step. The absence of major complications in this series of 115 consecutive laparoscopies certainly supports the safety of the procedure. The low morbidity, short hospital stay and minimal time required appeal particularly to the working mother. The absence of an abdominal scar is also much appreciated. The procedure, when used for investigation of infertility, provides far more information than other current methods.  相似文献   

17.
Appropriate surgical treatment of diverticulitis of the colon can result in a highly satisfactory proportion of permanent cures, with an operative mortality of 2 to 5 per cent. Colostomy is a valuable emergency procedure for the control of severe infection or the relief of obstruction but is not a satisfactory definitive treatment for the cure of diverticulitis. Definitive surgical treatment requires resection of the involved segment of colon. Three indications for surgical intervention in "uncomplicated" diverticulitis are: (1) continued or repeated attacks, (2) persistent deformity as seen by x-ray examination, and (3) persistent blood in the stools. Although a three-stage operation is usually considered a safer procedure, one-stage resections may be safely and satisfactorily employed in many instances.  相似文献   

18.
In this article, the authors introduce a new procedure for the treatment of high common peroneal nerve palsy. The principle of this technique consists of the neurotization of the anterior tibial nerve (deep peroneal nerve) with the bundle composed of the nerves to the soleus and lateral head of gastrocnemius muscles. The authors used this procedure for eight children who had permanent common peroneal nerve palsy caused by the injection of diclofenac in the gluteal region and for a 25-year-old male patient whose common peroneal nerve was transected near the gluteal region by a stab wound. For the cases in which paralysis was less than 8 months in duration, the results are satisfactory.  相似文献   

19.
A new permanent staining procedure for sister chromatid differentiation (SCD) in cultured Chinese hamster ovary cells has been established by combining the three-way differentiation in third mitosis (M3) chromosomes and the immunoperoxidase reaction developed with 3,3'-diaminobenzidine using a monoclonal anti-bromodeoxyuridine (BrdU) antibody. This procedure allows SCD at very low BrdU concentrations, and the evaluation of the sister chromatid exchange frequencies on a per cell-cycle basis.  相似文献   

20.
Pin worms can be cleared and differentiated in one operation after formalin fixation and dioxane dehydration by using carbol xylene. The entire procedure, including fixation time, requires less than 3 days. The mounts are permanent and give sufficient detail for class study.  相似文献   

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