Development of telial head in Ravenelia species

Development and cytology of the telial head in Ravenelia sessilis Berkeley, R. taslimii Mundkurs and R. ornata Sydow are traced in the ontogenic sequence. The species parasitic on leguminous hosts show some variations among themselves in the development of the telial heads and from those parasitizing euphorbiaceous hosts.     

中国团黑粉菌属和团散黑粉菌属

本文报道我国团黑粉菌属10种,团散黑粉菌属22种。其中新组合8种,即:水蔗草团散黑粉[Sporisorium apludae (H. Sydow & Sydow) L. Guo]、营大孢团散黑粉[Sporisorium benguetensis (Zundel) L. Guo]、细柄草团散黑粉[Sporisorium capillipedii (Ling) L. Guo]、营小孢团散黑粉[Sporisorium exsertum (McAlp.) L. Guo]、芒粒团散黑粉[Sporisorium miscanthi (Yen) L. Guo]、黄茅粒团散黑粉[Sporisorium moniliferum (Ell. & Ev.) L. Guo]、光高粱团散黑粉[Sporisorium taianum (Syd.) L. Guo]和鸭嘴草团散黑粉[Sporisorium tanglinensis (Tracy&Earle) L. Guo]。     

Effect of leaf age and inoculum concentration on the symptoms produced by Gymnosporangiumjuniperi-virginianae on apple

Gymnosporangium juniperi-virginianae, the incitant of cedar apple rust, induced the greatest number of lesions (flecks, pycnial lesions, aecial lesions) on 6- and 8-day-old leaves of single-shoot potted apple plants inoculated and incubated under controlled conditions. The largest pycnial lesions developed on 2- and 4-day-old leaves and the largest aecial lesions on 6-day-old leaves. The number of lesions was positively correlated with the concentration of basidiospores in the inoculum, but lesion size was constant at different inoculum levels. Elevated inoculum concentrations failed to induce pycnia on cv. McIntosh which normally bears only flecks; very low concentrations induced the development of pycnia but not aecia on cv. Rome Beauty, which normally bears aecia. An ‘infection rating’, log₁₀ (10 nd²), where n is the highest number of pycnial lesions per leaf and d is the largest mean diameter of pycnial lesions on any leaf, calculated for four cultivars in greenhouse tests showed a positive correlation with a ‘susceptibility rating’ developed from observations of natural infection of these cultivars in the field.     

In vitro germination of the prosorus and sexuality in four synchytrium species

In vitro germination of evanescent prosori scooped out of the sporangial galls of Synchytrium akshaiberi Lingappa parasitic on Solanum melongena L., S. rytzii Sydow on Anisomeles ovata R. Br., S. launeae Lingappa on Launea asplenifolia Hook. f. and S. sesamicola Lacy on Sesamum indicum L. was observed and studied in sequence. Anomalies observed in the sporangiogenesis and zoosporogenesis were discussed. Sexuality between the planogametes represented by their pairing, copulation and fusion was observed to be similar in the 4 species and described for S. sesamicola.     

Phylogenetic studies in <Emphasis Type="Italic">Ravenelia esculenta</Emphasis> and related rust fungi

Ravenelia esculenta Naras. and Thium. is a rust fungus, which infects mostly thorns, inflorescences, flowers and fruits of Acacia eburnea Willd. Aecial stages of the rust produce hypertrophy in infected parts. DNA of the rust fungus was isolated from aeciospores by ‘freeze thaw’ method. 18S rDNA was amplified and sequenced by automated DNA sequencer. BLAST of the sequence at NCBI retrieved 96 sequences producing significant alignments. Multiple sequence alignment of these sequences was done by ClustalW. Phylogenetic analysis was done by using MEGA 3.1. UPGMA Minimum Evolution tree with bootstrap value of 1000 replicates was constructed using these sequences. From phylogenetic tree it is observed that Ravenelia esculenta and the genus Gymnosporangium share a common ancestry, though Ravenelia esculenta is autoecious on angiosperm and the genus Gymnosporangium is heteroecious with pycnia, aecia on angiosperm and uredia, telia on gymnosperm. Two major clades are recognized which are based on the nature of aecial host (gymnosperm or angiosperm). These clades were also showing shift from pteridophytes to angiosperms as telial hosts. The tree can be interpreted in the other way also where there is separation of 14 families of Uredinales depending upon nature of teliospores, nature of aeciospores and structure of pycnia. These studies determine the phylogenetic position of Ravenelia esculenta among other rust fungi besides broad separation of Uredinales into two clades. These studies also show that there is phylogenetic correlation between molecular and morphological data. This is first report of DNA sequencing and phylogenetic positioning in genus Ravenelia from India.     

细叶蒿草炭黑粉菌新种及中国黑粉菌补遗VII

本文报道炭黑粉菌一新种,即细叶蒿草炭黑粉菌(Anthracoidea filifoliae L. Guo sp.nov.)和4个我国新记录种:1)角孢炭黑粉菌(A. angulata(H. Sydow) Boidol & Poclt), 2)黍状苔炭黑粉菌(A. paniceae Kukkenen), 3)狼尾草团黑粉菌(Sorosporium penniseti Mundkur)和4)米勒粘孢黑粉菌(Tolyposporium muellerianum (Thuemen) McAlpine)。     

Aphanocladiutn album, a fungus inducing teliospore production in rusts

The hyphomycetous fungus Aphanocladiutn album can grow over and around uredia of the rusts Puccinia coronata, P. hordei, P. graminis f.sp. avenae and P. recondita f.sp. triticina when host plants are kept under very humid conditions, but not on such plants not infected with rusts; uredia are adversely affected and telia develop in their vicinity. Plants inoculated with these rusts and with five isolates of A. album (one from a dead insect) showed: (1) much earlier development of telia on detached and non-detached rusted leaves inoculated with A. album than on corresponding leaves not thus inoculated; (2) telial induction by A. album in some isolates of rust species which hitherto had rarely or never produced telia; (3) precocious telial formation, in comparison with controls, when A. album spores were sprayed on leaves as much as 3 days before and 9 days after rust inoculation, and occasionally after uredia had already matured. As affected leaves remained green until the whole leaf became moribund, senescence is apparently not the factor inducing telia formation. The normal-appearing teliospores of some isolates were induced to germinate, whereas others did not. Rhamnus palaestina inoculated with basidiospores of one isolate of A. album-treated P. coronata f.sp. avenae produced pycnia and fertile aecia. The importance of A. album as a working tool in rust research and as a possible means for biological control of rust epiphytotics is discussed.     

Changes in Chemical Constituents of Tobacco Leaves Infected with Corynespora cassiicola and Colletotrichum nicotianae

The following smut fungi reported from Hyparrhenia hirta were studied: Sporisorium andropogonis, S.bardnonense, S. puellare, and S. transfissum. Two new combinations are proposed: Sporisorium barcinonense (Riofrio) Vanky, and S. puellare (H. Sydow) G. Deml. S. andropogonis and S. puellare have morphologically identical spores but differ in the type of spore germination. While S. puellare germinates by a ramified mycelium, S. andropogonis produces four-celled basidia forming basidiospores laterally and apically. It appears that S. andropogonis is restricted to Dichanthium ischaemum, and S. puellare to Hyparrhenia hirta. S. harcinonense and S. transfissum, both producing the same type of floral infection, are differentiated by their spore measurements.     

Taxonomy and phylogeny of Puccinia lagenophorae: a study using rDNA sequence data, morphological and host range features

Puccinia lagenophorae is a rust fungus originating from Australasia which has spread throughout the world. A phylogenetic analysis of taxa related to this species was performed using rDNA (LSU, ITS) sequence data. The analyses revealed a well-supported cluster including all specimens of P. lagenophorae. By evaluating morphological and sequence data, the species is taxonomically re-defined and a list of synonyms is provided. Puccinia distincta on Bellis perennis, a species recently separated from P. lagenophorae, P. saccardoi, a species on the Goodeniaceae, and P. byliana, a species so far only known from South Africa, are reduced to synonymy in P. lagenophorae, as are several other species. Our analysis indicates that P. lagenophorae is likely not derived from the northern hemisphere species P. obscura, but from a species from Australia host-alternating between Asteraceae (aecial host) and Cyperaceae/Juncaceae (telial host). Another related species, P. stylidii (on Stylidium sp., Stylidiaceae) may have been derived from the same parental species as P. lagenophorae. From ontogenetical and morphological studies, the presence of pycnia could not be confirmed in the life cycle of this species, and the width of the pedicel of teliospores at the point of attachment was found to be highly variable and not a taxonomic character. The number of known host species is approximately 150, including 41 new host plants recorded herein.     

中国黑粉菌补遗Ⅴ

摘要:本文报道我国黑粉菌五种新纪录。1)刺蓼轴黑粉菌(Sphacelotheca polygoni-senticosi(P. Hennings) Miyabe & Takahashi)寄生于假长尾叶蓼(Polygonum longisetum De Bruyn)和长尾叶蓼(Polygonum posumbu Hamilt.)。此种与水蓼轴黑粉菌(Sphacelotheca hydropiperis(Schum.) de Bary)的区别是黑粉孢子表面纹饰瘤状,常连接为脊状或不完全网状。2)七筋菇条黑粉菌(Urocystis clintoniae (Komarov) Vánky)寄生于七筋菇(Clintonia udensis Trautv. &Mey.)和扭柄花(Streptopus obtusatus Fassett)。3)宫部条黑粉菌(Urocystis miyabeana Togashi& Onuma)寄生于轮叶黄精(Polygonatum verticillatum (L.) All.)。4)喜马拉雅黑粉菌(Ustilagohimalensis (Kakishima & Ono) Vánky & Oberwinkler)寄生于细穗支柱蓼(Polygonum suffultumMaxim. var. pergracile (Hemsl.) G. Sam.)。5)伊朗黑粉菌(Ustilago iranica H. Sydow)寄生于远东友友草(Achnatherum extremiorientale (Hara) Keng)。     

Synonomy of the yeast generaHansenula andPichia demonstrated through comparisons of deoxyribonucleic acid relatedness

The relationship between the generaHansenula andPichia was examined through comparisons of DNA relatedness among phenotypically similar species.Hansenula minuta andPichia lindneri showed 75% DNA base sequence complementarity. In other comparisons,H. nonfermentans was found to share nearly 50% of its DNA sequences with bothH. minuta andP. lindneri. Because of the high degree of relatedness observed, it is proposed that ability to assimilate nitrate, the sole distinction betweenHansenula andPichia, is of insufficient taxonomic value for the reliable separation of either species or genera. Hat-spored species ofHansenula H. et P. Sydow 1919 are being transferred toPichia Hansen 1904. Species ofHansenula andPichia with Saturn-shaped ascospores will be transferred to the genusWilliopsis. The mention of firm names or trade products does not imply that they are endorsed or recommended by the U.S. Department of Agriculture over other firms or similar products not mentioned.     

African Hepatics. XXX: The genus Radula Dumortier

Abstract

Fifteen species of Radula from Africa and the Mascarenes are described, of which R. pseudoflaccida is new. The following species are newly reduced to synonyms: R. angustata Steph., R. capensis Steph., R. guineensis Steph., R. macroloba Steph., R. molleri Steph., R. pirottae Gola and R. spongiosa Steph. R. caespitosa Steph. is transferred from R. tabularis Steph. to R. madagascariensis Gottsche.

Habit, stem-structure, leaf-cuticle, perianth and spores provide valuable taxonomic characters which have received little or no attention and which show some degree of correlation. The range of forms shown by the lobule in a single species represent stages in its development.     

On the subdivision of the genusCeratocystis

The desirability is discussed of a subdivision of the genusCeratocystis into a group characterized by the presence of a phialidic conidial state, and a group in which the conidia are usually produced exogenously. Corroborative evidence for this arrangement is found in the carbohydrate constitution of the cells: in all examined species of the former group (Ceratocystis s.str.) rhamnose and cellulose are absent, whereas in the latter both components are present (Ophiostoma H. et P. Sydow).     

雄性不育无籽刺梨花药发育过程中生理特性的初步研究

为探究无籽刺梨(Rosasterilis)雄性不育原因,采用1%I2-KI染色法观察花粉活性,并对无籽刺梨和正常可育刺梨(R.roburghii)花药不同发育时期的生理生化指标进行了研究。结果表明,无籽刺梨的败育花粉占95.5%,刺梨的正常花粉占99%。刺梨花药的可溶性蛋白质、可溶性糖和脯氨酸含量在各时期的总体变化趋势相似,可溶性淀粉含量呈上升趋势,而无籽刺梨花药的可溶性蛋白质、可溶性糖、淀粉和脯氨酸含量在各时期的变化无规律可循,且花粉成熟期这4种物质的含量均明显低于刺梨,即花粉成熟期缺少各营养物质的积累。在花药发育过程中,无籽刺梨的SOD活性均低于刺梨;MDA含量呈上升趋势,且上升幅度比刺梨大;MDA含量和POD活性均高于刺梨。因此,营养物质的匮乏和酶系统的紊乱可能是导致无籽刺梨雄性不育的原因。     

Developing compound eye in lozenge mutants of Drosophila: lozenge expression in the R7 equivalence group

 The lozenge locus is genetically complex, containing two functionally distinct units, cistrons A and B, that influence the structure of the compound eye. Extreme mutations of either cistron produce adult phenotypes that share similarities and that have striking differences. We have analyzed the expression of several developmentally important eye genes including boss, scabrous, rhomboid, seven-up, and Bar in lozenge mutant backgrounds representing both cistrons. This analysis follows the progressive recruitment of photoreceptor neurons during eye development and has confirmed that the initial development of photoreceptors is normal up to the five cell precluster stage (R8, R2/5 and R3/4). However, when lozenge is mutant, further eye development is perturbed. As cells R1, R6 and R7 are recruited, patterns of gene expression for seven-up and Bar become abnormal. We have also characterized the expression of two different enhancer trap alleles of lozenge. The lozenge product(s) appear to be first expressed in the eye disc in undifferentiated cells shortly after the five cell precluster forms. Then, as distinct cells are recruited to a fate, lozenge expression persists and is refined in those cells. Our data suggests that lozenge functions in cone cells and pigment cells as well as in specific glia. With respect to photoreceptor neurons, lozenge biases the developmental potential of cells R1, R6 and R7, by directly influencing the expression of genes important for establishing cell fate. Received: 26 July 1996 / Accepted: 6 January 1997     

Book reviews

Nordperuanische Keramik. Gerdt Kutscher. “Monumenta Americana”, I. Ibero‐Amerikanische Bibliothek zu Berlin. Verlag Gebr. Mann, Berlin, 1955. 80 pp., in German and Spanish, 80 pls.

Geschichte der altamerikanischen Kulturen. H. D. Disselhoff. Verlag von R. Oldenbourg, München, 1953. 376 pp., 40 pls., 25 text figs., 10 maps.

Cenalora. Leben, Glaube, Tanz und Theater der Urvölker. Oskar Eberle. Ölten und Freiburg im Breisgau, 1954. 576 pp., 32 pls., 8vo.

American Indians in the Pacific. The Theory behind the Kon‐Tiki Expedition. Thor Heyerdahl. Stockholm 1952. 821 pp., 90 pls., 52 text figs., 11 maps.

Die Kulturen der aussereuropäischen Erdteile in Übersicht. Führer durch die Schausammlungen des Instituts für Völkerkunde Universität Göttingen. Hans Plischke et al. Göttingen 1954. 101 pp., 10 pls.

Orfebrería prehispánica de Colombia. José Pérez de Barradas. I‐II. Madrid, 1954. 367 pp., 300 pls.

Afrikanische Plastik. Eckhart von Sydow. Aus dem Nachlass herausgegeben von Gerdt Kutscher. Verlag Gebr. Mann, Berlin, 1954. 177 pp., 144 pls.

The Northern States of Fiji. A. M. Hocart. Royal Anthropological Institute. Occasional Publication No. 11. London, 1952. 304 pp., 2 maps.

Native Money of Palau. Robert E. Ritzenthaler. Milwaukee Public Museum, Publications in Anthropology, No. 1. Milwaukee, Wise, 1954. 46 pp.

The World of Primitive Man. Paul Radin. New York, 1953. 370 pp.     

Generation and characterization of inner photoreceptor-specific enhancer-trap lines using a novel piggyBac-Gal4 element in Drosophila

The Drosophila inner photoreceptors R7 and R8 are responsible for color vision and their differentiation starts at the third instar larval stage. Only a handful of genes with R7 or R8-cell-specific expression are known. We performed an enhancer-trap screen using a novel piggyBac transposable element, pBGay, carrying a Gal4 sequence under the control of the P promoter to identify novel genes expressed specifically in R7 or R8 cells. From this screen, three lines were analyzed in detail: piggyBac^AC109 and piggyBac^AC783 are expressed in R8 cells and piggyBac^AC887 is expressed in R7 cells at the third instar larval stage and pupal stages. Molecular analysis showed that the piggyBac elements were inserted into the first intron of CG14160 and CG7985 genes and the second intron of unzipped. We show the expression pattern in the developing eye imaginal disc, pupal retina as well as the adult retina. The photoreceptor-specific expression of these genes is reported for the first time and we propose that these lines are useful tools for studying the development of the visual system.     

Species delineation in the genus Nadsonia Sydow

The genus Nadsonia Sydow is revised on the basis of morphology, physiology, amino acid and fatty acid composition, electrophoretic patterns of some enzymes and DNA relatedness. Two species, N. commutata (type CBS 6640) and N. fulvescens, with two varieties, N. fulvescens var. fulvescens (type CBS 2596) and N. fulvescens var. elongata (type CBS 2594) nov. comb. are recognized. A modified diagnosis of the genus and a key are given.Parts of this work were presented at the IXth International Specialized Symposium on Yeasts (Smolenice, SSR, April 18–22, 1983); VIth General Symposium on Yeasts (Montpellier, France, July 9–13, 1984) and International Symposium The Expanding Realm of Yeast-like Fungi (Amersfoort, The Netherlands, August 3–7, 1987).     

Features of Crossability, Haploidy and Polyembryony in Hybrid Combinations between Cultivated Barley Hordeum vulgare L. (2n = 14) and Wheat-Rye Substitution Lines Triticum aestivum L., Cultivar Saratovskaya 29/Secale cereale L., Cultivar Onokhoiskaya

The role of individual chromosomes of rye in the manifestation of crossability and seedling development in hybrid combinations between cultivated barley Hordeum vulgare L., cultivar Nepolegayushchii (2n = 14) and five wheat-rye substitution lines Triticum aestivum L., cultivar Saratovskaya 29/Secale cereale L., cultivar Onokhoiskaya (2n = 40 wheat + 2 rye chromosomes). Crossability, which was measured by two parameters—frequency of set grains and frequency of grains with embryos—was shown to be significantly affected by each of the five rye chromosomes examined: 1R, 2R, 3R, 5R, and 6R; the development of barley haploids was affected by rye chromosomes 1R, 3R, and 5R. We were the first to demonstrate that polyembryony could be induced by mutual effects of barley cytoplasm and rye chromosome 1R. Possible mechanisms controlling the development of haploids and twins in hybrid combinations H. vulgare × T. aestivum/S. cereale are discussed. The conclusion is drawn that hybrid combinations between cultivated barley and wheat-rye substitution lines can serve as new models for studying incompatibility mechanisms in distant crosses and genetic control of parthenogenesis.__________Translated from Genetika, Vol. 41, No. 6, 2005, pp. 784–792.Original Russian Text Copyright © 2005 by Pershina, Belova, Devyatkina, Rakovtseva, Kravtsova, Shchapova.