共查询到20条相似文献,搜索用时 46 毫秒
1.
2.
3.
4.
5.
6.
7.
Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence 总被引:2,自引:0,他引:2
Litovchick L Sadasivam S Florens L Zhu X Swanson SK Velmurugan S Chen R Washburn MP Liu XS DeCaprio JA 《Molecular cell》2007,26(4):539-551
The mammalian Retinoblastoma (RB) family including pRB, p107, and p130 represses E2F target genes through mechanisms that are not fully understood. In D. melanogaster, RB-dependent repression is mediated in part by the multisubunit protein complex Drosophila RBF, E2F, and Myb (dREAM) that contains homologs of the C. elegans synthetic multivulva class B (synMuvB) gene products. Using an integrated approach combining proteomics, genomics, and bioinformatic analyses, we identified a p130 complex termed DP, RB-like, E2F, and MuvB (DREAM) that contains mammalian homologs of synMuvB proteins LIN-9, LIN-37, LIN-52, LIN-54, and LIN-53/RBBP4. DREAM bound to more than 800 human promoters in G0 and was required for repression of E2F target genes. In S phase, MuvB proteins dissociated from p130 and formed a distinct submodule that bound MYB. This work reveals an evolutionarily conserved multisubunit protein complex that contains p130 and E2F4, but not pRB, and mediates the repression of cell cycle-dependent genes in quiescence. 相似文献
8.
9.
p53-dependent down-regulation of telomerase is mediated by p21waf1 总被引:13,自引:0,他引:13
Shats I Milyavsky M Tang X Stambolsky P Erez N Brosh R Kogan I Braunstein I Tzukerman M Ginsberg D Rotter V 《The Journal of biological chemistry》2004,279(49):50976-50985
10.
11.
12.
13.
Histone deacetylase inhibitors: signalling towards p21cip1/waf1 总被引:2,自引:0,他引:2
Ocker M Schneider-Stock R 《The international journal of biochemistry & cell biology》2007,39(7-8):1367-1374
14.
15.
16.
17.
Smith E Redman RA Logg CR Coetzee GA Kasahara N Frenkel B 《The Journal of biological chemistry》2000,275(26):19992-20001
Unique cell cycle control is instituted in confluent osteoblast cultures, driving growth to high density. The postconfluent dividing cells share features with cells that normally exit the cell cycle; p27(kip1) is increased, p21(waf1/cip1) is decreased, free E2F DNA binding activity is reduced, and E2F4 is primarily nuclear. E2F4-p130 becomes the predominant E2F-pocket complex formed on E2F sites, but, unlike the complex that typifies resting cells, cyclin A and CDK2 are also present. Administration of dexamethasone at this, but not earlier stages, results in reduction of cyclin A and CDK2 levels with a parallel decrease in the associated kinase activity, dissociation of cyclin A-CDK2 from the E2F4-p130 complexes, and inhibition of G(1)/S transition. The glucocorticoid-mediated cell cycle attenuation is also accompanied by, but not attributable to, increased p27(kip1) and decreased p21(waf1/cip1) levels. The attenuation of osteoblast growth to high density by dexamethasone is associated with severe impairment of mineralized extracellular matrix formation, unless treatment commences in cultures that have already grown to high density. Both the antimitotic and the antiphenotypic effects are reversible, and both are antagonized by RU486. Thus, glucocorticoids induce premature attenuation of the osteoblast cell cycle, possibly contributing to the osteoporosis induced by these drugs in vivo. 相似文献
18.
19.
20.
Taylor WR DePrimo SE Agarwal A Agarwal ML Schönthal AH Katula KS Stark GR 《Molecular biology of the cell》1999,10(11):3607-3622