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癫痫大鼠与正常大鼠脑中钙调神经磷酸酶及其底物的研究 总被引:5,自引:0,他引:5
报道了听源性癫痫大鼠发作后其脑内钙调神经磷酸酶(Calcineurin,CaN)及其底物蛋白磷酸化水平的改变,以PNPP为底物测CaN的活力,用间接ELISA测CaN的含量,SDS-PAGE和2-D-PAGE并放射自显影的方法研究脑内蛋白质磷酸化水平,发现与正常大鼠相比,听源性癫痫大鼠发作后,脑内CaN的含量并没有改变,但比活力下降,其底物的磷酸化状态也有改变,其中一个30kD蛋白磷酸化程度明显降 相似文献
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报道了听源性癫痫大鼠发作后其脑内钙调神经磷酸酶(Calcineurin,CaN)及其底物蛋白磷酸化水平的改变。以PNPP为底物测CaN的活力,用间接ELISA测CaN的含量,SDS-PAGE和2-D-PAGE并放射自显影的方法研究脑内蛋白质磷酸化水平,发现与正常大鼠相比,听源性癫痫大鼠发作后,脑内CaN的含量并没有改变,但比活力下降,其底物的磷酸化状态也有改变,其中一个30kD蛋白磷酸化程度明显降低。实验结果提示,大鼠听源性癫痫与CaN及其调控的底物有相关性。 相似文献
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于大鼠皮下埋入含醋酸去氧皮质酮(DOCA)的硅胶管以形成DOCA-salt高血压,其中一组动物在埋管前切除(T_9-L_2)脊髓右侧背根神经,每周以尾套法测定大鼠收缩压,埋管后6周测定大鼠脑和血浆中儿茶酚胺(CA)和血管紧张素Ⅱ(AngⅡ)的浓度,用电脑血管显微图像分析系统测量血管的结构变化。与对照鼠相比,DOCA-salt高血压大鼠下丘脑和延脑的肾上腺素含量和AngⅡ放免活性及血浆去甲肾上腺素(NE)浓度均明显增加,血浆AngⅡ浓度降低,心系数(心重/体重)和肠系膜动脉的中层厚度、壁厚与腔径(壁腔)比值增大;切除脊髓背根可明显延缓DOCA-salt高血压的形成、预防以上组织CA浓度和AngⅡ放免活性增加以及缓解心肌和血管平滑肌的肥厚。提示,肾神经传入纤维在DOCA-salt高血压形成中起作用,其机制可能通过影响脑内肾上腺素能神经元和激活脑肾素-血管紧张素系统增加交感传出而起作用。 相似文献
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大鼠鞘内注射多巴胺受体激动剂与拮抗剂对痛及针刺镇痛的影响 总被引:6,自引:0,他引:6
在大鼠电刺激甩测痛模型上,应用鞘内注射(it)多巴胺(DA)受体选择性激动剂与拮抗剂,分析大鼠脊髓DA受体亚型D1和D2在痛及针刺镇痛(AA)中的作用。结果显示,在正常清醒大鼠,it D2受体选择性激动剂,Y171555(LY)或D1/D2受体激动剂阿朴吗啡(APO)有镇痛作用(呈剂量依赖式增加),并加强AA,而it D1受体选择性激动剂SKF38393(SKF)对痛及AA均无影响;it D1受体 相似文献
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目的:探讨在抗体应答期间,脑和淋巴器官中儿茶酚胺(CAs)含量的动态变化,籍以了解免疫状态对中枢和外周CAs神经活动的影响。方法:用绵羊红细胞(SRBC)免疫大鼠,在免疫后第2 ̄7d应用高效液相色谱-电化学检测法(HPLC-ECD)测定大鼠下丘脑、海马、脑干和胸腺中云甲肾上腺素(NA)、肾上腺素(A)、多巴胺(DA)和高香草酸(HVA)的含量。结果:①下且脑和海马内NA在抗体应答期间升高,而胸腺中 相似文献
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目的:探讨实验性变态反应性脑脊髓炎(EAE)时,中枢和外周儿茶酚胺水平的变化规律,为神经系统自身免疫性疾病的发病机制和治疗的进一步研究提供实验依据。方法:用豚鼠脊髓加完全弗氏佐剂的佐剂乳化物诱导Wistar大鼠发生EAE。在EAE2级和3级临床症状时,用高效液相色谱-电化学检测法(HPLC-ECD)测定下丘脑、海马、肠系膜淋巴结和血清中去甲肾上腺素(NA)、多巴胺(DA)和肾上腺素(A)的含量。F 相似文献
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于大鼠皮下埋入含醋酸去氧皮质酮(DOCA)的交管以形成DOCA-salt高血压,其中一组动物在埋管前切除(T9-L2)脊髓右侧背根神经,每周以尾套法测定大鼠收缩压,埋管后6周测定大鼠脑和血浆中儿茶酚胺(CA)和血管紧张素Ⅱ(AngⅡ)的浓度,用电脑血管显微图像分析系统测量血管的结构变化。与对照鼠相比,DOCA-salt高血压大鼠下丘脑和延脑 肾上腺素含量和AngⅡ放免活性及血浆去甲肾上腺素(NE) 相似文献
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电针对大鼠脑内雌激素受体蛋白及其mRNA表达的影响 总被引:24,自引:1,他引:23
本文应用放射免疫分析(RIA)、RNA点杂交和Northern blot、单克隆抗体免疫组织化学和计算机图像处理技术研究电针对切除卵巢大鼠脑内雌激素受体(ER)蛋白和mRNA表达的影响。以探讨针刺作用的分子生物学机理。结果表明,切除卵巢可导致血雌二醇(E2)水平降低,动物脑内ER蛋白和mRNA的表达增强;电针实验穴位后,去卵巢大鼠血的E2含量明显增加,脑内ER蛋白和mRNA表达受到明显抑制。正常大 相似文献
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Michael Hesse 《Plant Systematics and Evolution》1980,134(3-4):229-267
Some closely related members of the monocotyledonous familiesAlismataceae, Liliaceae, Juncaceae, Cyperaceae, Poaceae andAraceae with variable modes of pollination (insect- and wind-pollination) were studied in relation to the ultrastructure of pollenkitt and exine (amount, consistency and distribution of pollenkitt on the surface of pollen grains). The character syndromes of pollen cementing in entomophilous, anemophilous and intermediate (ambophilous or amphiphilous) monocotyledons are the same in principal as in dicotyledons. Comparing present with former results one can summarize: 1) The pollenkitt is always produced in the same manner by the anther tapetum in all angiosperm sub-classes. 2) The variable stickiness of entomophilous and anemophilous pollen always depends on the particular distribution and consistency of the pollenkitt, but not its amount on the pollen surface. 3) The mostly dry and powdery pollen of anemophilous plants always contains a variable amount of inactive pollenkitt in its exine cavities. 4) A step-by step change of the pollen cementing syndrome can be observed from entomophily towards anemophily. 5) From the omnipresence of pollenkitt in all wind-pollinated angiosperms studied one can conclude that the ancestors of anemophilous angiosperms probably have been zoophilous (i.e. entomophilous) throughout. 相似文献
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Surveillance of Class Ⅰ Newcastle Disease Virus at Live Bird Markets and Commercial Poultry Farms in Eastern China Reveals the Epidemic Characteristics 下载免费PDF全文
Xiaolong Lu Xiaoquan Wang Tiansong Zhan Yifan Sun Xin Wang Naiqing Xu Tianxing Liao Yu Chen Min Gu Shunlin Hu Xiaowen Liu Xiufan Liu 《中国病毒学》2021,36(4):818-822
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正Dear Editor,Parainfluenza virus 5 (PIV5), known as canine parainfluenza virus in the veterinary field, is a negative-sense,nonsegmented, single-stranded RNA virus belonging to the Paramyxoviridae family (Chen 2018). The virus was first reported in primary monkey kidney cells in 1954 (Hsiung1972), then it has been frequently discovered in various 相似文献
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Altaf Hussain Tiantian Wu Hui Li Linjin Fan Kai Li Li Gao Yongqiang Wang Yulong Gao Changjun Liu Hongyu Cui Qing Pan Yanping Zhang Asim Aslam Khan Muti-Ur-Rehman Muhammad Munir Salman Latif Butt Xiaomei Wang Xiaole Qi 《中国病毒学》2019,34(1):102-105
<正>Dear Editor,Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector.However, there is limited genome information of IBDV 相似文献
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Jiaming Li Yidun Zhang Lina Jiang Hongliang Cheng Jingjing Li Li Li Zehui Chen Fei Tang Yingying Fu Yifei Jin Bing Lu Jing Zheng Zhongyi Wang 《中国病毒学》2022,37(5):762-764
Highlights
1 Aerosol emission rates of Delta or Omicron patients were similar.
2 Viral loads in upper respiratory tract of Alpha, Delta and Omicron patients were similar.
3 Viral loads in upper respiratory tract of vaccinated or unvaccinated Delta patients had no difference. 相似文献
1 Aerosol emission rates of Delta or Omicron patients were similar.
2 Viral loads in upper respiratory tract of Alpha, Delta and Omicron patients were similar.
3 Viral loads in upper respiratory tract of vaccinated or unvaccinated Delta patients had no difference. 相似文献
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Danrong Shi Keda Chen Xiangyun Lu Linfang Cheng Tianhao Weng Fumin Liu Nanping Wu Lanjuan Li Hangping Yao 《中国病毒学》2022,37(2):295-298
Highlights
1) A comprehensive evaluation method for anti-SARS-CoV-2 drugs was established based on RT-qPCR, TCID50 method, and immunofluorescence.
2) A significant antiviral effect of rHuIFN-α1b was shown with EC50=0.12 IU/mL in Vero cells and EC50=0.52 IU/mL in Calu-3 cells, which was better than rHuIFN-α2b (EC50=0.25 IU/mL in Vero cells and EC50=2.48 IU/mL in Calu-3 cells).
3) rHuIFN-α1b has a good potential in the application of anti-COVID-19 therapy. 相似文献
1) A comprehensive evaluation method for anti-SARS-CoV-2 drugs was established based on RT-qPCR, TCID50 method, and immunofluorescence.
2) A significant antiviral effect of rHuIFN-α1b was shown with EC50=0.12 IU/mL in Vero cells and EC50=0.52 IU/mL in Calu-3 cells, which was better than rHuIFN-α2b (EC50=0.25 IU/mL in Vero cells and EC50=2.48 IU/mL in Calu-3 cells).
3) rHuIFN-α1b has a good potential in the application of anti-COVID-19 therapy. 相似文献