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 共查询到19条相似文献,搜索用时 78 毫秒
1.
报道了中国兰科植物一未详知种——喉红石斛Dendrobium christyanum Rchb.f.,提供了其形态描述和墨线图。它在形态上与矮石斛Dbellatulum Rolfe相似,但其唇瓣白色,唇盘中央橘红色,侧裂片和中裂片基部有黄色晕而与后者不同。这两种植物隶属石斛属黑毛组Sect Formosae。  相似文献   

2.
记载了在福建省发现的兰科石斛属植物一新变种——文卉石斛Dendrobium luoi var. wenhuii W. L. Yang,并描述其与原变种罗氏石斛D. luoi L. J. Chen et W. H. Rao和近缘种D. zhenghuoense S. P. Chen, L. Ma & M. H. Li的形态特征区别。  相似文献   

3.
西双版纳地区的石斛资源调查及鉴定   总被引:3,自引:1,他引:2  
对石斛主产区之一--云南西双版纳的药源调查和商品鉴定结果表明,有22种石斛属、1种金石斛属及1种石仙桃属植物加工成石斛商品,其中昌帽石斛Dendrobium crystallinum Rchb.f.,流苏石斛D.fimbriatum Hook。囊唇石斛D.moschatum Sw及鼓槌石斛D.chrysotoxum Lindl.为主要品种。  相似文献   

4.
道地药材霍山石斛及其相似种的分子鉴定   总被引:3,自引:1,他引:2  
为检测霍山石斛与霍山产铜皮石斛、霍山产铁皮石斛及其它产地的几种药用石斛的差异,对霍山石斛及其相似种进行ISSR分子标记比较研究。结果表明,尽管霍山石斛及其相似种的外部形态差异较小,但在ISSR分子标记指纹图谱上却存在着显著而稳定的差异。因此,根据ISSR分子标记指纹图谱所显示的多态性位点差异,能准确鉴别霍山石斛及其相似种植物及药材。此方法简单、稳定性高、可重复性好,在霍山石斛的分子鉴定研究中有较大的应用价值。  相似文献   

5.
描述了四川夹江(兰科)石斛属植物一新种,即夹江石斛。  相似文献   

6.
利用解剖镜和扫描电镜,对我国9种石斛属(Dendrobium)植物的花药帽形态和超微特征进行观察。结果显示,花药帽的颜色、形态、大小和外壁纹饰特征在种间差异明显。9种植物的花药帽按颜色可分为:黄药帽、白药帽、紫药帽;按极轴和赤道轴比值可分为:长球形和半球形。扫描电镜观察结果发现,花药帽外壁纹饰有4种类型:扁平-光滑、扁平-条纹、乳突-条纹和乳突-光滑。本研究初步探讨了9种石斛花药帽形态的分类学意义,支持以下观点:(1)石斛组是一个多系和并系;(2)球花石斛(D. thyrsiflorum Rchb. F.)和密花石斛(D. densiflorum Lindl. ex Wall)是一对近缘种;(3)杯鞘石斛(D. gratiosissimum Rchb. F.)和大苞鞘石斛(D.wardianum Warner)是一对近缘种,且与喇叭唇石斛(D. lituiflorum Lindl.)亲缘关系较近。  相似文献   

7.
河南石斛属植物的调查研究   总被引:16,自引:1,他引:15       下载免费PDF全文
河南产石斛属(Dendrobium)植物发现有6种。本文论述了石斛属植物的生态环境特点、生物学特性及其繁殖的方法。同时根据本省的实际状况,提出了开发利用石斛属植物资源的几点建议。  相似文献   

8.
应用RAPD分子标记技术探讨3种石斛属植物的种间关系   总被引:2,自引:0,他引:2  
采用 RAPD 分子标记技术,分析了金钗石斛、铁皮石斛和齿瓣石斛三种石斛属植物的种间关系。10个引物产生的113条DNA扩增片段中,106条(93.81%)具有多态性,利用113个RAPD标记,计算遗传距离,利用非加权组平均法建立聚类图。结果表明,RAPD标记技术较好地从分子水平揭示金钗石斛、铁皮石斛和齿瓣石斛三种石斛属植物的遗传背景、亲缘关系,并为后期在DNA水平上对药用石斛的开发利用提供资料。  相似文献   

9.
兰科石斛属植物菌根真菌研究进展   总被引:1,自引:0,他引:1  
石斛属(Dendrobium)隶属于兰科(Orchidaceae)树兰亚科(Epidendroideae)石斛兰族(Dendrobiinae),是兰科最大的属之一,终生附生于树上或岩石上。石斛属很多种类具有很高的药用价值与观赏价值。由于人为过度采挖和野生生境的破坏,使得野生石斛资源濒临灭绝。石斛属植物为典型的兰科菌根植物,在自然条件下需要与真菌共生,才能完成生活史。菌根真菌对于石斛属植物的种子萌发和植株生长具有重要的作用。对石斛属植物菌根的形成、菌根真菌的作用、菌根真菌多样性及菌根技术在石斛属植物中的应用做了评述,并对今后的研究内容和重点提出了一些思路。  相似文献   

10.
国产金石斛属植物小志   总被引:1,自引:0,他引:1       下载免费PDF全文
本文报道了产于我国的兰科金石斛属植物9种1变种,其中有4个新种和1新变种。  相似文献   

11.
About 63 species of Dendrobium are identified in China, making the identification of the origin of a particular Dendrobium species on the consumer market very difficult. We report evaluation of multiple species-specific probes screened from genomic DNA for closely related Dendrobium species identification, based on DNA array hybridization. Fourteen species-specific probes were screened from five closely related Dendrobium species, D. aurantiacum Kerr, D. officinale Kimura et Migo, D. nobile Lindl., D. chrysotoxum Lindl. and D. fimbriatum Hook., based on the SSH-Array technology we developed. Various commercial Dendrobium samples and unrelated samples were definitely identified. The specificity and accuracy of the multiple species-specific probes for species identification was assessed by identifying various commercial Dendrobium samples (Herba Dendrobii). Hybridization patterns of these multiple probes on digested genomic DNAs of Dendrobium species indicated that there are distinct polymorphic sequence fragment in the higher eukaryotes. This is the first report on detection and utilization of multiple species-specific probes of Dendrobium in whole genomic DNA, and this could be useful tools not only for a new technical platform for the closely related species identification but also for epidemiological studies on higher eukaryotes.  相似文献   

12.
Genomlc DNA polymorphlsms are very useful for tracing genetic traits end studying biological diversity among species. Here, we present a method we call the "diversity suppresslon-subtractlve hybridization array" for effectively profiling genomlc DNA polymorphisms. The method first obtains the subtracted gDNA fragments between any two species by suppression subtraction hybridization (SSH) to establish e subtracted gDNA library, from which diversity SSH arrays are created with the selected subtracted clones. The diversity SSH array hybridizes with the DIG-labeled genomlc DNA of the organism to be assayed. Six closely related Dendrobium species were studied as model samples. Four Dendrobium species as testers were used to perform SSH. A total of 617 subtracted positive clones were obtained from four Dendrobium species, and the average ratio of positive clones was 80.3%. We demonstrated that the average percentage of polymorphlc fragments of palrwlse comparisons of four Dendrobium species was up to 42.4%. A dendrogram of the relatedness of six Dendrobium species was produced according to their polymorphic profiles. The results revealed that the diversity SSH array Is a highly effective platform for profiling genomlc DNA polymorphlsms and dendrograms.  相似文献   

13.
Plant phylogeny constrains orchid mycorrhizal(OrM) fungal community composition in some orchids. Here, we investigated the structures of the OrM fungal communities of eight Dendrobium species in one niche to determine whether similarities in the OrM fungal communities correlated with the phylogeny of the host plants and whether the Dendrobium-OrM fungal interactions are phylogenetically conserved. A phylogeny based on DNA data was constructed for the eight coexisting Dendrobium species,and the OrM fungal communities were characterized by their roots. There were 31 different fungal lineages associated with the eight Dendrobium species. In total, 82.98% of the identified associations belonging to Tulasnellaceae, and a smaller proportion involved members of the unknown Basidiomycota(9.67%). Community analyses revealed that phylogenetically related Dendrobium tended to interact with a similar set of Tulasnellaceae fungi. The interactions between Dendrobium and Tulasnellaceae fungi were significantly influenced by the phylogenetic relationships among the Dendrobium species. Our results provide evidence that the mycorrhizal specificity in the eight coexisting Dendrobium species was phylogenetically conserved.  相似文献   

14.
Dendrobium shompenii is described and illustrated as a new species from Great Nicobar Island, India. Affinities with the closely related species Dendrobium nathanielis are discussed.  相似文献   

15.
当前已开发的石斛(Dendrobium)SSR标记仅100余对,难以满足研究的需要。为开发更多石斛分子标记,本研究通过生物信息学方法从公共核酸数据库搜索石斛SSR。结果表明,GenBank收录的3599条石斛DNA序列经拼接获得1343条Uni—DNA序列。经搜索,共检测出283个SSR,分布于205条Uni—DNA序列,平均每2815bp含一个SSR。通过序列比对,剔除86条已开发引物的SSR—DNA序列,从剩余的119条序列设计76对引物,并在石斛属32个种间进行可转移性分析,结果显示47对引物得到有效扩增,种间可转移率为51.1%N95.7%,平均为75.9%。有效扩增引物中有46对在石斛种间具多态性,检测出等位基因数2~8个,平均4.0个。选取10对多态性引物扩增60份铁皮石斛资源,每对引物检测出等位基因数2~5个,平均3.4个。根据SSR扩增带型将60份铁皮石斛资源聚为5大类,同一类型内的表型较类群间接近。对DMl21扩增产物测序表明铁皮石斛种内的SSR等位变异由SSR重复次数差异造成,而石斛种间的SSR等位变异还包含SSR位点侧翼序列的插入缺失以及替换。  相似文献   

16.
兰属、兜兰属、石斛属植物叶片的扫描电镜观察   总被引:10,自引:0,他引:10  
对兰科植物的兰属、兜兰属及石斛属16个种折叶片及其横断面进行了扫描电镜的观察。兰属各种叶片上表皮细胞均为矩形,上表皮细胞表面具小乳突或不明显突起。石斛属及兜兰属的各个种上下表皮细胞均为多边形,但石斛属表皮细胞表面无坦无纹饰,而兜兰属花叶类上表皮细胞表面明显呈乳突状,绿叶类呈龟背状隆起。兰属及石斛属叶片叶肉组织没有栅栏组织及海绵组织的分化,而兜兰属的绿叶类叶肉不分化;花叶类叶肉有分化。  相似文献   

17.
石斛作为传统的中药材,种类繁多,分布广泛,具有增强免疫力、抗氧化、抗肿瘤以及养阴生津等功效。抗氧化活性在石斛的药理功能中占据重要地位,能抑制活性氧对机体的损伤,延缓衰老,预防疾病,大量研究均围绕石斛这一特性展开。本文从抗氧化活性的石斛种、评价方法与有效成分及其作用机制三方面对石斛进行综述,以期为学者的相关研究提供最新参考。  相似文献   

18.
菌根真菌─新种─—石斛小菇(英文)   总被引:3,自引:0,他引:3  
从云南省野生铁皮石斛(Dendrobiumcandidumwall.ExLindl.)根中分离出一菌根真菌,经系统形态学研究后,将其鉴定为小菇属(Mycena)一新种:石斛小菇(Mycenadendrobii)。标本保藏在中国科学院微生物研究所菌物标本室(HMAS)。用石斛小菇伴播12种兰科植物种子,实验结果表明该真菌对天麻(Gastrodiaelata)和密花石斛(Dendrobiumdensdlorum)种子萌发有明显促进作用。  相似文献   

19.
Dendrobium wangliangii G. W. Hu, C. L. Long & X. H. Jin, a new species from the north of the Yunnan Province in China is described and illustrated. The morphological differences between the new species and the related species, Dendrobium flexicaule , and its endangered status are discussed.  © 2008 The Linnean Society of London, Botanical Journal of the Linnean Society , 2008, 157 , 217–221.  相似文献   

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