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Transcriptional elements involved in the repression of ribosomal protein synthesis. 总被引:12,自引:0,他引:12 下载免费PDF全文
Baojie Li Concepcion R. Nierras Jonathan R. Warner 《Molecular and cellular biology》1999,19(8):5393-5404
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De Sanctis V La Terra S Bianchi A Shore D Burderi L Di Mauro E Negri R 《Journal of molecular biology》2002,318(2):333-349
We have analyzed in detail the structure of RAP1-UAS(RPG) complexes in Saccharomyces cerevisiae cells using multi-hit KMnO(4), UV and micrococcal nuclease high-resolution footprinting. Three copies of the Rap1 protein are bound to the promoter simultaneously in exponentially growing cells, as shown by KMnO(4) multi-hit footprinting analysis, causing extended and diagnostic changes in the DNA structure of the region containing the UAS(RPG). Amino acid starvation does not cause loss of Rap1p from the complex; however, in vivo UV-footprinting reveals the occurrence of structural modifications of the complex. Moreover, low-resolution micrococcal nuclease digestion shows that the chromatin of the entire region is devoid of positioned nucleosomes but is susceptible to changes in accessibility to the nuclease upon amino acid starvation. The implications of these results for the mechanism of Rap1p action are discussed. 相似文献
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Multiple factors bind the upstream activation sites of the yeast enolase genes ENO1 and ENO2: ABFI protein, like repressor activator protein RAP1, binds cis-acting sequences which modulate repression or activation of transcription. 总被引:30,自引:14,他引:16 下载免费PDF全文
P K Brindle J P Holland C E Willett M A Innis M J Holland 《Molecular and cellular biology》1990,10(9):4872-4885