Nitrogen nutrition of ginger (Zingiber officinale)

Summary Two fertilizer experiments were conducted in the field at Beerwah, South-East Queensland. In the first experiment leaf nitrogen concentrations, and the yield of ginger shoots and rhizomes at early and late harvests increased both with the total amount of nitrogen applied up to the highest level studied (336 kg N/ha as ammonium nitrate) and with the number of applications making up the total. At all levels of nitrogen application the apparent recovery of fertilizer nitrogen increased in the order 1 application <2 applications <4 applications. At 33.6 kg N/ha there appeared to be no advantage in dividing the total N applied into more than 4 applications but the data suggested higher recoveries of nitrogen with 8 applications at 112 kg N/ha and 336 kg N/ha. In the second experiment, ammonium nitrate, urea, and ammonium sulphate were found to be equally effective as nitrogen fertilizers for ginger when applied at equal rates of nitrogen per hectare. However, in terms of cost effectiveness they rated in the order urea > ammonium nitrate > ammonium sulphate.All three nitrogen sources acidified the soil, the decrease in soil pH during the growing season increasing with increasing rate of application. In Experiment 1 split applications, which increased the recovery of applied nitrogen in the crop, also increased the extent of acidification. In Experiment 2 ammonium sulphate tended to be more strongly acidifying than the other fertilizers but the difference was statistically significant only at the highest rate of nitrogen application. Because of the strong effects of nitrogen supply on both yield and soil pH, the highest yields were associated with end-of-season pH values below 5.0.     

Differential sensitivity to ozone in two poplar clones.

The effect of acute O₃ exposure (5 h at 150 ppb) on photosynthetic electron transport and the thylakoid polypeptide pattern has been studied in 2-month-old cuttings of two poplar clones ( Populus deltoides × maximowiczii clone Eridano and P .× euramericana clone I-214), known for their differential response to O₃ in terms of visible symptoms. The more sensitive Eridano developed severe blackish bifacial necrosis concentrated in the interveinal areas of the basal and intermediate leaves, while in the less sensitive I-214 clone, visible symptoms were slight or entirely absent. A generalized, significant decrease in leaf pigment content was shown by both of the stressed clones, although the decline in β -carotene content was much more marked in Eridano than in I-214. Activation of the xanthophyll cycle was not detected in either clone. In both clones, immunoblot analysis of the thylakoid proteins showed a decrease in the levels of all the PSII polypeptides tested following O₃ exposure, whereas the PsaA/B protein in the PSI core was more severely affected in Eridano than in I-214. No changes in the chloroplast structure could be seen on electron microscopic study in either clone. The only visible difference was a great accumulation of starch grains in Eridano. All of these results suggest that O₃ exposure induces alterations in the photosynthetic apparatus in both poplar clones, Eridano being affected to a greater extent than I-214.     

Comparison of growth, biomass and nutrient distribution in five promising clones of Populus deltoides under an agrisilviculture system

Variations in growth, above- and below-ground biomass and nutrient distribution were examined in five clones (G3, G48, 65/27, D121 and S7C1) of Populus deltoides grown under agrisilviculture system in sub-humid tropics of Central India. The monoclonal blocks were planted at 4x5 m in a randomized block design with three replications. Diameter at breast height (dbh) and tree height were consistently higher in clone 65/27 and lowest in clone S7C1. Mean annual increments (MAI) in dbh and height were 1.6 and 1.3 times higher in clone 65/27 compared to clone S7C1. Total biomass varied from 48.5 to 62.2 Mg ha(-1) in six-year-old clones. In rank order, the total biomass of clones was: 65/27>D121>G48>G3>S7C1. Stem wood accounted 60.4-68.9% to total biomass followed by coarse roots (12.2-18.9%), branches (12.3-15%), leaves (3.02-6.9%) and fine roots (1.5-2.7%). Root-shoot ratio ranged from 0.2 to 0.35. It was highest in clone G48 and lowest in clone S7C1. In six-year-old clones, total N ranged from 184.3 to 266.3 kg ha(-1), P from 16.8 to 31.1 kg ha(-1) and K from 81.9 to 128.7 kg ha(-1). Total N and P were highest in clone 65/27, while K in clone G48. Nutrients were lowest in clone S7C1. In general, maximum nutrients (N, P and K) were allocated to above-ground components (leaves>stem>branches) than below-ground components. Available N, P and K in the soil improved significantly after six years of planting. It was higher in 0-20 cm and decreased with soil depth. At 0-20 cm soil depth, N increased from 14.9% to 24.1%, P from 17.2% to 23.3% and K from 3.1% to 5.1% under different clones. The yield of both soybean and wheat reduced under poplar clones. Yield losses in soybean ranged from 10.1% to 33% and wheat from 15% to 30.3% under different clones. The management strategies for reducing tree-crop competition and nutrient export from the site under P. deltoides based agrisilviculture system for achieving sustainable production are discussed.     

Do tree-ring traits reflect different water deficit responses in young poplar clones (Populus × canadensis Mönch ‘I-214’ and P. deltoides ‘Dvina’)?

Poplar clones are known to display a wide range of tolerance to drought and water-use efficiency, but the effects of water deficit on stem growth and tree-ring characteristics are rarely taken into account. This study was conducted in order to investigate whether the main tree-ring traits correlate with irrigation regimes during the growing season in ‘I-214’ and ‘Dvina’ 4-year-old poplar clone saplings grown in concrete tanks, during three consecutive years. Total carbon, stable carbon isotope, Klason lignin and α-cellulose contents were analyzed to characterize wood biochemistry; ring width, wood density, mean vessel density and mean vessel lumen area were analyzed to characterize wood anatomy to assess the influence of irrigation regime. In both clones, wood formed in 2005 was more enriched in ¹³C, suggesting drought-induced stomatal closure. Wood formed in 2006 was less variable in δ¹³C in relation to irrigation regimes. ‘Dvina’ showed higher Klason lignin content and wood density than ‘I-214’, whatever the irrigation regime, despite the larger ring widths. ‘Dvina’ has the potential to recover promptly after drought stress, but at the expense of poor wood technological properties, while ‘I-214’ could continue to grow more uniformly under limited water availability, though at a lower rate.     

Effect of spacing and nitrogen fertilization on the establishment and biomass production of short rotation poplar in Finland

Short rotation trials cuttings poplar (Populus x rasumowskyana) in Southern Finland investigated establishment of poplar plantations and the effects of spacing and application of nitrogen fertilizer on biomass production over a period of 6 years. Thicker cuttings grew better whilst those of less than 1 cm diameter grew only moderately. Nitrogen fertilization improved height and diameter growth and above-ground dry mass yield. Woody biomass production was 4·2 dry tons/ha per year, at 300 kg/ha nitrogen. A spacing of 15 000 stems/ha gave the best yield after 4 years, but 5000 stems/ha was a more productive spacing in the next 2 years.     

Early responses to cadmium of two poplar clones that differ in stress tolerance

Soil cadmium (Cd) contamination is becoming a matter of great global concern. The identification of plants differentially sensitive to Cd excess is of interest for the selection of genotype adaptive to grow and develop in polluted areas and capable of ameliorating or reducing the negative environmental effects of this toxic metal. The two poplar clones I-214 (Populus × canadensis) and Eridano (Populus deltoides × maximowiczii) are, respectively, tolerant and sensitive to ozone (O₃) exposure. Because stress tolerance is mediated by an array of overlapping defence mechanisms, we tested the hypothesis that these two clones differently sensitive to O₃ stress factor also exhibit different tolerance to Cd. With this purpose, an outdoor pot experiment was designed to study the responses of I-214 and Eridano to the distribution of different Cd solutions enriched with CdCl₂ (0, 50 and 150 μM) for 35 days. Changes in leaf area, biomass allocation and Cd uptake, photosynthesis, chlorophyll fluorescence, leaf concentration of nutrients and pigments, hydrogen peroxide (H₂O₂) and nitric oxide (NO) production and thiol compounds were investigated. The two poplar clones showed similar sensitivity to excess Cd in terms of biomass production, photosynthesis activity and Cd accumulation, though physiological and biochemical traits revealed different defence strategies. In particular, Eridano maintained in any Cd treatment the number of its constitutively wider blade leaves, while the number of I-214 leaves (with lower size) was reduced. H₂O₂ increased 4.5- and 13-fold in I-214 leaves after the lowest (L) and highest (H) Cd treatments, respectively, revealing the induction of oxidative burst. NO, constitutively higher in I-214 than Eridano, progressively increased in both clones with the enhancement of Cd concentration in the substrate. I-214 showed a more elevated antioxidative capacity (GSH/GSSG) and higher photochemical efficiency of PSII (F_v/F_m) and de-epoxidation degree of xantophylls-cycle (DEPS). The glutathione pool was not affected by Cd treatment in both clones, while non-protein thiols and phytochelatins were reduced at L Cd treatment in I-214. Overall, these two clones presented high adaptability to Cd stress and are both suitable to develop and growth in environments contaminated with this metal, thus being promising for their potential use in phytoremediation programmes.     

The effect of fertilizer‐N on the inter‐specific competition among three wheat aphids under elevated CO2

Increasing atmospheric carbon dioxide (ab. CO₂) and fertilizer‐nitrogen (ab. N) applications may have marked direct effects on the plant growth of agricultural crops, and in turn affect the higher trophic level of insect herbivores. In this study, the effects of elevated CO₂ (i.e., 650 µl/L vs. ambient 400 µl/L) and fertilizer‐N (0, 50, 100, 200 kg/ha) on the population abundances and the inter‐specific competition among three co‐occurring species of wheat aphids, Sitobion avenae, Rhopalosiphum padi and Schizaphis graminum, were studied. The grain weight per ear and the 1,000‐grain weight were generally increased when grown under elevated CO₂ and showed a significant effect at the 100 kg/ha (grain weight per ear) and 0, 50 and 100 kg/ha (1,000‐grain weight) N. These two yield indexes increased with increasing fertilizer‐N levels within reasonable limits and reached a maximum at 100 kg/ha. Elevated CO₂ combined with fertilizer‐N levels formed complex indirect effects on the three wheat aphids through the wheat crops they fed on. Elevated CO₂ significantly decreased the niche overlap index (ab. NOI) between S. avenae and R. padi under 0 and 100 kg/ha and that between R. padi and S. graminum under 0 kg/ha, while significantly increased the three NOIs under 50 kg/ha and that between R. padi and S. graminum under 100 and 200 kg/ha. S. avenae and R. padi had the larger population and stronger competition in low‐N condition (0 and 50 kg/ha), which was harmful to wheat yield and quality when combined with its own poor nutrition. Overall, the 100 kg/ha N level was the best option based on the aphid population, competition and wheat yields. Therefore, the balanced relationship formed among fertilizers, plants and insects under 100 kg/ha N was vital for the interactive ecosystem.     

Effect of Chronic O3 Fumigation on the Activity of Some Calvin Cycle Enzymes in Two Poplar Clones

The effects of long-term exposure to ozone (O₃, 60 mm³ m^-3 for 5 h d^-1) on some Calvin cycle enzymes, in particular those modulated by the thioredoxin system, were studied in two poplar clones. These clones differ in sensitivity to O₃. In the I-214 clone, the first effects from O₃ treatment were seen after 40 d of fumigation, while the Eridano clone showed visible symptoms of damage after only 15 d of the treatment. Specific activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (E.C. 4.1.1.39) diminished in both the clones, while specific activity of phosphoenolpyruvate carboxylase (E.C. 4.1.1.31) increased. Exposure to O₃ also caused a reduction in the specific activity of ribulose-1,5-bisphosphate kinase (E.C. 2.7.1.19) in both clones. At the end of the exposure to O₃, specific activity of glyceraldehyde 3-phosphate dehydrogenase (E.C. 1.2.1.13) increased in I-214 and remained similar to the control in Eridano, whereas specific activity of fructose-1,6-bisphosphate phosphatase (E.C. 3.1.3.11) was higher in Eridano and similar to the control in I-214.     

Relative efficiency of N fertilizers as influenced by N-serve in the potato crop

Summary Field experiments, on potato, were conducted in the alluvial soils of Ludhiana, in 1976–77 and 1980–81, to investigate the relative efficiency of N fertilizers as influenced by a nitrification inhibitor (N-serve). In the absence of N-serve, sulphate of ammonia (S/A) and calcium ammonium nitrate (CAN) proved superior to urea, on an equal N basis, in almost all the yield characters and yield, but urea treated with N-serve tended to equal S/A and proved better than CAN. The inhibitor increased the N-uptake and N-recovery from urea and decreased the optimum basal dose of the fertilizer, by 10.8kg N/ha in 1976–77 and by 40.5 kg N/ha in 1980–81, without reducing the tuber yield in any of the two years. S/A and CAN did not profit from the treatment with N-serve.     

Influence of different fertilizers on nett gain or loss of soil nitrogen in long-term manure and fertilizer applications

Summary The nett gain or loss of total soil nitrogen was worked out from a long-term manure and fertilizer experiment conducted for seven years and still continuing at Ranchi Agricultural College, Kanke, Ranchi India. The total nitrogen in soil showed a deficit balance where lower and unbalanced doses of fertilizers were applied. Increasing levels of fertilizer combinations with lime removed the highest amount of nitrogen in intensive cropping. The nett loss was highest (95 kg/ha) in case of control, at 100% NPK the nett gain was 37 kg N per ha, and at 150% NPK 72 kg N per ha which was the highest.     

The response ofPinus caribaea mor. var. Hondurensis seedlings to nitrogen,phosphorous and potassium fertilizers

Summary A soil pot culture experiment was conducted to study the response ofPinus caribaea var. hondurensis seedlings to the N, P, K fertilizers in a 3³ factorial combination of nutrients in four replicates. For this purpose, seedlings were grown in plastic pots in soils collected from Bahau pine experimental area. These soils belong to Durian series and are generally poor in nutrients.The seedlings were supplied with 112 kg/ha, 336 kg/ha and 560 kg/ha (100 lb/ac, 300 lb/ac and 500 lb/ac) of N, P, K in solution form in all possible combinations. Periodic growth measurements were taken and dry matter production was estimated at the conclusion of the experiment.The results indicate that phosphorous is the most important nutrient for the height growth of seedlings. The absolute height increment under the best treatment combination (N₁P₃K₁) was about threefolds (25.7cm) compared to 8.8 cm in the control. It was also found that higher levels of N and K, though not beneficial to height growth, resulted in better radial growth and more dry matter production compared to control plants. However, highest levels of N and K in the present experiment produced inhibitory influence as regards height growth even in the presence of low level of phosphorous.The study indicates that application of phosphorous at least at the rate of 560 kg/ha per plant is necessary on this type of soil to boost the initial height growth ofPinus caribaea var. hondurensis. Nitrogen at the rate of 336 kg/ha and potassium at the rate of 112 kg/ha will induce better radial growth.     

Effects of 60-year N,P, K and Ca fertilization on EUF-nutrient fractions in the soil and on yields of rye and potato crops

Summary In field experiments carried out uninterruptedly since 1923 on a sandy, grey-brown luvisol (8% clay) the EUF-nutrient fractions were determined after potato and rye crops in 1982 had been harvested.Annual applications of N fertilizer (90 kg N/ha) over a 60-year period raised both the organic and inorganic EUF-N fractions (Table 1). EUF-N_org values increased depending on crop rotation from 1.1–1.9 to 2.4–3.6 mg/100 g. This increase in EUF-N amounts was higher under rye monoculture than under potato monoculture. N leaching was more intensive under potato monoculture than under rye monoculture.Application of P, K and Ca fertilizers raised the EUF-P, EUF-K and EUF-Ca values at 20°C as well as at 80°C (Figs. 1, 2, 3). Analyses of soil samples from the 25–75 cm layer showed that the contents of EUF-Ca and EUF-K in this layer are as high as in the topsoil or even higher (Table 3).High yields of rye (>4 t/ha) and of potatoes (>30 t/ha) were obtained when in samples taken after harvest EUF-amounts of 3.5 mg N, 2 mg P, 3 mg K (in both topsoil and subsoil), 1.7 mg Mg and 20 mg Ca/100 g were released within 35 min. These levels were obtained with annual fertilizer application rates of 90 kg N, 60 kg P₂O₅ and 110 kg K₂O/ha.Applications of 1.6 t CaO/ha every 4 years were sufficient to maintain the soil pH at 6.0–6.5. Calculated on the basis of EUF-Ca contents, with the amounts of K-selective minerals being taken into account, this rate of liming corresponds with the needs of acid sandy soils.     

Nitrogen cycling in dense plantings of hybrid poplar and black alder

Summary Nitrogen cycling was studied during the third growing season in pure and mixed plantings (33×33 cm spacing) of hybrid poplar and black alder in southeastern Canada. After 3 years, hybrid poplar growth and N content of living tissues in a plot and of individual hybrid poplar plants increased with the proportion of black alder in a planting. No differences were detected among N contents of individual alder plants regardless of plot treatment. Black alder allocated a larger portion of its N to roots than hybrid poplar. Symbiotic nitrogen fixation was estimated to account for 80% of the nitrogen in aboveground alder tissues in the pure treatment using natural¹⁵N dilution. N return in leaf litter was estimated to be 70kg ha^–1 in the pure alder treatment and decreased to a minimum of 20 kg ha^–1 in the pure hybrid poplar plots. No difference was detected among treatments for throughfall N content. Nitrogen concentration in roots and leaf litterfall of black alder was higher than hybrid poplar. Significant soil N accretion occurred in mixed plantings containing two alders to one poplar and pure black alder plantings. Nitrogen availability (NO₃–N) increased with the amount of black alder in a plot. Results suggest that the early increase in nitrogen accumulation of hybrid poplar in mixed treatments can be attributed to an increase of total soil N availability resulting from the input of large amounts of N from easily mineralizable alder tissue.     

Leaching losses of nitrogen and potassium in polders reclaimed from Lake Ijssel

Summary Mineral nitrogen and potassium lost from the Eastern Flevoland polder and from the North Eastern polder were estimated at regular intervals by determining the amounts of soluble nitrogen and potassium in the water which had been admitted by locks and inlets and discharged by pumping stations and subtracting nitrogen and potassium added in the rain. From 1959 to 1966 the nett annual losses of nitrogen in the discharged water from various pumping stations in Eastern Flevoland ranged from 18.9 to 33.2 (average 25.2) kg N per ha. Similarly in the North Eastern polder from 1963 to 1966 the nett annual losses ranged from 16.7 to 33.2 (average 22.1) kg N per ha. Rainfall added annually between 4.8 and 11.8 (average 8.6) kg N per ha to both polders. Nett losses from Eastern Flevoland ranged from 9.2 to 21.9 (average 15.8) kg per ha and from the North Eastern polder from 10.1 to 24.0 (average 14.9) kg N per ha, annually. Drainage water contained principally nitrate nitrogen, whereas the discharged water in many cases contained both ammonium and nitrate nitrogen, and often more ammonium than nitrate, presumably because the composition of the mineral nitrogen in the waterways is changed by the growth and death of algae. From 1962 to 1966 the discharged water removed potassium from the Eastern Flevoland polder in amounts ranging from 105 to 226 (average 162) kg K per ha annually. During 1963–1964, amounts lost from the North Eastern polder ranged from 47 to 71 (average 59) kg K per ha each year. The rain added from 4.5 to 10.7 (average 6.9) kg K per ha annually to both polders. Nett losses from Eastern Flevoland ranged from 99 tot 219 (average 156) kg K per ha and from the North Eastern polder from 41 to 67 (average 54) kg K per ha annually.     

Trichoderma spp. and Bacillus spp. as growth promoters in maize (Zea mays L.)

Microbes that are beneficial to plants are used to enhance the crop growth, yield and are alternatives to chemical fertilizers. Trichoderma and Bacillus are the predominant plant growth-promoting fungi and bacteria. The objective of this study was select, characterize, and evaluate isolates of Trichoderma spp. and Bacillus spp. native from the northern region of Sinaloa, Mexico, and assess their effect on growth promotion in maize (Zea mays L.). In greenhouse conditions, four Trichoderma isolates and twenty Bacillus isolates, as well as two controls, were tested in a completely randomized design with three replicates. We selected the two best strains of Trichoderma and Bacillus: TB = Trichoderma asperellum, TF = Trichoderma virens, B14 = Bacillus cereus sensu lato and B17 = Bacillus cereus, which were evaluated in the field in a completely randomized blocks in factorial arrangement design with three replicates applying different rates of nitrogen fertilizer (0, 150 kg N/ha, and 300 kg N/ha). Treatments 5 (B17 = B. cereus) and 11 (TF = T. virens) both fertilized with 150 kg N/ha showed similar yields and they did not reveal significant differences from the treatments fertilized with 300 kg N/ha. This indicated that treatment 5 (B17= B. cereus with 150 kg N/ha) and treatment 11 (TF= T. virens with 150 kg N/ha) were efficient as growth promoters, by not showing significant differences in root volume and dry weight of foliage. The results indicated a reduction of 50% in the rate of nitrogen to fertilizer required for maize (Zea mays L.) crops. These microorganisms Trichoderma and Bacillus could be an alternative to reduce the use of chemical fertilizers in maize.     

The influence of organic fertilizers,obtained from poplar barks,on the correction of iron chlorosis ofLupinus albus L.

Summary The influence exerted by organic matter on the correction of iron chlorosis inLupinus albus L., grown in calcareous soil, was studied through a pot experiment by incorporating two rates (1.5 per cent and 3 per cent on the soil) of three organic fertilizers obtained from poplar barks. The fertilizers differed in the length of humification process and in the integration with different materials,i.e.: H fertilizer: 8 months humified poplar barks without any addition, HF fertilizer: 4 years humified poplar barks with 4 per cent of metallic iron, HP fertilizer: 18 months humified poplar barks with 25 per cent of poultry manure. The higher rate (3 per cent) of the H fertilizer was the most efficient in reducing chlorosis symptoms and gave the greatest dry matter yield. Among the treatments with the lower rate (1.5 per cent), the HF fertilizer gave the best results; conversely, the HP fertilizer failed to correct chlorosis and to enhance yield. Therefore the experiment suggested that organic fertilizers are useful to correct iron chlorosis, provided their phosphorus content is low and they have undergone a prolonged humification process; furthermore, integration with small amounts of iron leads to a considerable saving of organic fertilizer.     

Removal of nitrogen and phosphorus by Eucalyptus and Populus at a tertiary treated municipal wastewater sprayfield

Various progenies of Eucalyptus grandis and E. amplifolia, and clones of Populus deltoides, were evaluated for plant removal of nitrogen (N) and phosphorus (P) for 26 months at a municipal waste spray field in north Florida. Tertiary treated wastewater containing 2.73 mg L(-1) nitrate N and 0.30 mg L(-1) total P was applied using sprinkler irrigation (93.8 m3 ha(-1) d(-1)) to fast growing trees utilized for bioenergy. Eucalyptus amplifolia and E. grandis survived and grew very poorly as the result of severe winter injury in two successive years and were not evaluated for nutrient removal. Survival and growth of P. deltoides demonstrated suitability for phytoremediation, and selected clones were evaluated for biomass and nutrient content. Removals of total N (TN) and total P (TP) were greatest for main stem (36% and 44%, respectively) and foliage (44% and 36%, respectively). Low biomass producing clones generally had higher nutrient concentrations, but high biomass producing clones removed more TN and TP. Approximately 789 kg ha(-1) TN and 103 kg ha(-1) TP were removed by the highest biomass producing P. deltoides clone, representing 215% of N and 615% of P inputs.