Deep soil inventories reveal that impacts of cover crops and compost on soil carbon sequestration differ in surface and subsurface soils

Increasing soil organic carbon (SOC) via organic inputs is a key strategy for increasing long‐term soil C storage and improving the climate change mitigation and adaptation potential of agricultural systems. A long‐term trial in California's Mediterranean climate revealed impacts of management on SOC in maize‐tomato and wheat–fallow cropping systems. SOC was measured at the initiation of the experiment and at year 19, at five depth increments down to 2 m, taking into account changes in bulk density. Across the entire 2 m profile, SOC in the wheat–fallow systems did not change with the addition of N fertilizer, winter cover crops (WCC), or irrigation alone and decreased by 5.6% with no inputs. There was some evidence of soil C gains at depth with both N fertilizer and irrigation, though high variation precluded detection of significant changes. In maize?tomato rotations, SOC increased by 12.6% (21.8 Mg C/ha) with both WCC and composted poultry manure inputs, across the 2 m profile. The addition of WCC to a conventionally managed system increased SOC stocks by 3.5% (1.44 Mg C/ha) in the 0–30 cm layer, but decreased by 10.8% (14.86 Mg C/ha) in the 30–200 cm layer, resulting in overall losses of 13.4 Mg C/ha. If we only measured soil C in the top 30 cm, we would have assumed an increase in total soil C increased with WCC alone, whereas in reality significant losses in SOC occurred when considering the 2 m soil profile. Ignoring the subsoil carbon dynamics in deeper layers of soil fails to recognize potential opportunities for soil C sequestration, and may lead to false conclusions about the impact of management practices on C sequestration.     

Carbon sequestration potential in perennial bioenergy crops: the importance of organic matter inputs and its physical protection

To date, only few studies have compared the soil organic carbon (SOC) sequestration potential between perennial woody and herbaceous crops. The main objective of this study was to assess the effect of perennial woody (poplar, black locust, willow) and herbaceous (giant reed, miscanthus, switchgrass) crops on SOC stock and its stabilization level after 6 years from plantation on an arable field. Seven SOC fractions related to different soil stabilization mechanisms were isolated by a combination of physical and chemical fractionation methods: unprotected (cPOM and fPOM), physically protected (iPOM), physically and chemically protected (HC‐μs + c), chemically protected (HC‐ds + c), and biochemically protected (NHC‐ds + c and NHC‐μs + c). The continuous C input to the soil and the minimal soil disturbance increased SOC stocks in the top 10 cm of soil, but not in deeper soil layers (10–30; 30–60; and 60–100 cm). In the top soil layer, greater SOC accumulation rates were observed under woody species (105 g m^?2 yr^‐1) than under herbaceous ones (71 g m^?2 yr^‐1) presumably due to a higher C input from leaf‐litter. The conversion from an arable maize monoculture to perennial bioenergy crops increased the organic C associated to the most labile organic matter (POM) fractions, which accounted for 38% of the total SOC stock across bioenergy crops, while no significant increments were observed in more recalcitrant (silt‐ and clay‐sized) fractions, highlighting that the POM fractions were the most prone to land‐use change. The iPOM fraction increased under all perennial bioenergy species compared to the arable field. In addition, the iPOM was higher under woody crops than under herbaceous ones because of the additional C inputs from leaf‐litter that occurred in the former. Conversion from arable cropping systems to perennial bioenergy crops can effectively increase the SOC stock and enlarge the SOC fraction that is physically protected within soil microaggregates.     

Changes in soil carbon stocks under perennial and annual bioenergy crops

Bioenergy crops are expected to provide biomass to replace fossil resources and reduce greenhouse gas emissions. In this context, changes in soil organic carbon (SOC) stocks are of primary importance. The aim of this study was to measure changes in SOC stocks in bioenergy cropping systems comparing perennial (Miscanthus × giganteus and switchgrass), semi‐perennial (fescue and alfalfa), and annual (sorghum and triticale) crops, all established after arable crops. The soil was sampled at the start of the experiment and 5 or 6 years later. SOC stocks were calculated at equivalent soil mass, and δ¹³C measurements were used to calculate changes in new and old SOC stocks. Crop residues found in soil at the time of SOC measurements represented 3.5–7.2 t C ha^?1 under perennial crops vs. 0.1–0.6 t C ha^?1 for the other crops. During the 5‐year period, SOC concentrations under perennial crops increased in the surface layer (0–5 cm) and slightly declined in the lower layers. Changes in δ¹³C showed that C inputs were mainly located in the 0–18 cm layer. In contrast, SOC concentrations increased over time under semi‐perennial crops throughout the old ploughed layer (ca. 0–33 cm). SOC stocks in the old ploughed layer increased significantly over time under semi‐perennials with a mean increase of 0.93 ± 0.28 t C ha^?1 yr^?1, whereas no change occurred under perennial or annual crops. New SOC accumulation was higher for semi‐perennial than for perennial crops (1.50 vs. 0.58 t C ha^?1 yr^?1, respectively), indicating that the SOC change was due to a variation in C input rather than a change in mineralization rate. Nitrogen fertilization rate had no significant effect on SOC stocks. This study highlights the interest of comparing SOC changes over time for various cropping systems.     

Soil carbon increased by twice the amount of biochar carbon applied after 6 years: Field evidence of negative priming

Applying biochar to agricultural soils has been proposed as a means of sequestering carbon (C) while simultaneously enhancing soil health and agricultural sustainability. However, our understanding of the long‐term effects of biochar and annual versus perennial cropping systems and their interactions on soil properties under field conditions is limited. We quantified changes in soil C concentration and stocks, and other soil properties 6 years after biochar applications to corn (Zea mays L.) and dedicated bioenergy crops on a Midwestern US soil. Treatments were as follows: no‐till continuous corn, Liberty switchgrass (Panicum virgatum L.), and low‐diversity prairie grasses, 45% big bluestem (Andropogon gerardii), 45% Indiangrass (Sorghastrum nutans), and 10% sideoats grama (Bouteloua curtipendula), as main plots, and wood biochar (9.3 Mg/ha with 63% total C) and no biochar applications as subplots. Biochar‐amended plots accumulated more C (14.07 Mg soil C/ha vs. 2.25 Mg soil C/ha) than non‐biochar‐amended plots in the 0–30 cm soil depth but other soil properties were not significantly affected by the biochar amendments. The total increase in C stocks in the biochar‐amended plots was nearly twice (14.07 Mg soil C/ha) the amount of C added with biochar 6 years earlier (7.25 Mg biochar C/ha), suggesting a negative priming effect of biochar on formation and/or mineralization of native soil organic C. Dedicated bioenergy crops increased soil C concentration by 79% and improved both aggregation and plant available water in the 0–5 cm soil depth. Biochar did not interact with the cropping systems. Overall, biochar has the potential to increase soil C stocks both directly and through negative priming, but, in this study, it had limited effects on other soil properties after 6 years.     

Evolution of soil carbon stocks under Miscanthus × giganteus and Miscanthus sinensis across contrasting environmental conditions

Miscanthus is a C4 bioenergy perennial crop characterized by its high potential yield. Our study aimed to compare the carbon storage capacities of Miscanthus sinensis (M. sinensis) with that of Miscanthus × giganteus (M. × giganteus) in field conditions in different types of soils in France. We set up a multi‐environment experimental network. On each trial, we tested two treatments: M. × giganteus established from rhizomes (Gr) and M. sinensis transplanted seedlings (Sp). We quantified the soil organic carbon (SOC) stock at equivalent soil mass for both genotypes in 2014 and 2019 and for two sampling depths: L1 (ca. 0–5 cm) and L1‐2 (ca. 0–30 cm). We also calculated the total and annual variation of the SOC stock and investigated factors that could explain the variation and the initial state of the SOC stock. ANOVAs were performed to compare the SOC stock, as well as the SOC stock variation rates across treatments and soil layers. Results showed that the soil bulk density did not vary significantly between 2014 and 2019 for both treatments (Gr and Sp). The SOC concentration (i.e. SOC expressed in g/kg) increased significantly between 2014 and 2019 in L1, whereas no significant evolution was found in L2 (ca. 5–30 cm). The SOC stock (i.e. SOC expressed in t/ha) increased significantly in the superficial layer L1 for M. × giganteus and M. sinensis, by 0.48 ± 0.41 and 0.54 ± 0.25 t ha^?1 year^?1 on average, respectively, although no significant change was detected in the layer L1‐2 for both genotypes. Moreover, SOC stocks in 2019 did not differ significantly between M. × giganteus and M. sinensis in the soil layers L1 and L1‐2. Lastly, our results showed that the initial SOC stock was significantly higher when miscanthus was grown after set‐aside than after annual crops.     

Plant roots and GHG mitigation in native perennial bioenergy cropping systems

Native perennial bioenergy crops can mitigate greenhouse gases (GHG) by displacing fossil fuels with renewable energy and sequestering atmospheric carbon (C) in soil and roots. The relative contribution of root C to net GHG mitigation potential has not been compared in perennial bioenergy crops ranging in species diversity and N fertility. We measured root biomass, C, nitrogen (N), and soil organic carbon (SOC) in the upper 90 cm of soil for five native perennial bioenergy crops managed with and without N fertilizer. Bioenergy crops ranged in species composition and were annually harvested for 6 (one location) and 7 years (three locations) following the seeding year. Total root biomass was 84% greater in switchgrass (Panicum virgatum L.) and a four‐species grass polyculture compared to high‐diversity polycultures; the difference was driven by more biomass at shallow soil depth (0–30 cm). Total root C (0–90 cm) ranged from 3.7 Mg C ha^?1 for a 12‐species mixture to 7.6 Mg C ha^?1 for switchgrass. On average, standing root C accounted for 41% of net GHG mitigation potential. After accounting for farm and ethanol production emissions, net GHG mitigation potential from fossil fuel offsets and root C was greatest for switchgrass (?8.4 Mg CO2e ha^?1 yr^?1) and lowest for high‐diversity mixtures (?4.5 Mg CO2e ha^?1 yr^?1). Nitrogen fertilizer did not affect net GHG mitigation potential or the contribution of roots to GHG mitigation for any bioenergy crop. SOC did not change and therefore did not contribute to GHG mitigation potential. However, associations among SOC, root biomass, and root C : N ratio suggest greater long‐term C storage in diverse polycultures vs. switchgrass. Carbon pools in roots have a greater effect on net GHG mitigation than SOC in the short‐term, yet variation in root characteristics may alter patterns in long‐term C storage among bioenergy crops.     

The impacts of four potential bioenergy crops on soil carbon dynamics as shown by biomarker analyses and DRIFT spectroscopy

Perennial bioenergy crops accumulate carbon (C) in soils through minimally disturbing management practices and large root inputs, but the mechanisms of microbial control over C dynamics under bioenergy crops have not been clarified. Root‐derived C inputs affect both soil microbial contribution to and degradation of soil organic matter resulting in differing soil organic carbon (SOC) concentrations, storage, and stabilities under different vegetation regimes. Here, we measured biomarker amino sugars and neutral sugars and used diffuse reflectance mid‐infrared Fourier transform spectroscopy (DRIFTS) to explore microbial C contributions, degradation ability, and SOC stability, respectively, under four potential bioenergy crops, M.×giganteus (Miscanthus × giganteus), switchgrass (Panicum virgatum L.), a mixed prairie, and a maize (Zea mays L.)–maize–soybean (Glycine max(L.) Merr.) (MMS) rotation over six growing seasons. Our results showed that SOC concentration (g/kg) increased by 10.6% in mixed prairie over the duration of this experiment and SOC storage (Mg/ha) increased by 17.0% and 15.6% in switchgrass and mixed prairie, respectively. Conversion of row crops to perennial grasses maintained SOC stability and increased bacterial residue contribution to SOC in M.×giganteus and switchgrass by 20.0% and 15.0%, respectively, after 6 years. Degradation of microbe‐derived labile SOC was increased in M.×giganteus, and degradation of both labile and stable SOC increased in MMS rotation. These results demonstrate that microbial communities under perennial grasses maintained SOC quality, while SOC quantity increased under switchgrass and mixed prairie. Annual MMS rotation displayed decreases in aspects of SOC quality without changes in SOC quantity. These findings have implications for understanding microbial control over soil C quantity and quality under land‐use shift from annual to perennial bioenergy cropping systems.     

Sensitivity of soil carbon fractions and their specific stabilization mechanisms to extreme soil warming in a subarctic grassland

Terrestrial carbon cycle feedbacks to global warming are major uncertainties in climate models. For in‐depth understanding of changes in soil organic carbon (SOC) after soil warming, long‐term responses of SOC stabilization mechanisms such as aggregation, organo‐mineral interactions and chemical recalcitrance need to be addressed. This study investigated the effect of 6 years of geothermal soil warming on different SOC fractions in an unmanaged grassland in Iceland. Along an extreme warming gradient of +0 to ~+40 °C, we isolated five fractions of SOC that varied conceptually in turnover rate from active to passive in the following order: particulate organic matter (POM), dissolved organic carbon (DOC), SOC in sand and stable aggregates (SA), SOC in silt and clay (SC‐rSOC) and resistant SOC (rSOC). Soil warming of 0.6 °C increased bulk SOC by 22 ± 43% (0–10 cm soil layer) and 27 ± 54% (20–30 cm), while further warming led to exponential SOC depletion of up to 79 ± 14% (0–10 cm) and 74 ± 8% (20–30) in the most warmed plots (~+40 °C). Only the SA fraction was more sensitive than the bulk soil, with 93 ± 6% (0–10 cm) and 86 ± 13% (20–30 cm) SOC losses and the highest relative enrichment in ¹³C as an indicator for the degree of decomposition (+1.6 ± 1.5‰ in 0–10 cm and +1.3 ± 0.8‰ in 20–30 cm). The SA fraction mass also declined along the warming gradient, while the SC fraction mass increased. This was explained by deactivation of aggregate‐binding mechanisms. There was no difference between the responses of SC‐rSOC (slow‐cycling) and rSOC (passive) to warming, and ¹³C enrichment in rSOC was equal to that in bulk soil. We concluded that the sensitivity of SOC to warming was not a function of age or chemical recalcitrance, but triggered by changes in biophysical stabilization mechanisms, such as aggregation.     

Integrated agroforestry systems improve soil carbon storage,water productivity,and economic returns in the marginal land of the semi-arid region

Environmental crises, land degradation, and frequent crop failure threaten the livelihoods of millions of the populace in the semi-arid agroecosystems. Therefore, different combinations of annual crops with perennial fruit trees were assessed to restore the soil carbon, and enhance farm productivity and profitability in a semi-arid climate. The study hypothesized that the integration of perennial fruit trees with seasonal crops may enhance farm productivity, economic returns, and environmental sustainability. Integration of phalsa (Grewia asiatica) with mung bean (Vigna radiata) - potato (Solanum tuberosum) system recorded the highest system productivity (25.9 Mg/ha) followed by phalsa with cowpea (Vigna unguiculata) -mustard (Brassica juncea) systems (21.2 Mg/ha). However, Karonda (Carissa sp.) with mung bean - potato system recorded maximum net return (3529.1 US$/ha), and water use efficiency (33.0 kg/ha-mm). Concerning the benefit-cost (B:C) ratio, among the agroforestry systems, the karonda + cowpea - mustard system registered a maximum BC ratio (3.85). However, SOC density remained higher (9.10 Mg/ha) under the phalsa + cowpea - mustard and Moringa + mung bean - potato system (9.16 Mg/ha) over other systems. Similarly, phalsa + mung bean - potato system had the highest C sustainability index (27.6), carbon sequestration potential (0.6–0.67 Mg/ha/year), and water use efficiency (33.0 kg/ha-mm). Hence, the study suggested that the integration of short-duration leguminous and oilseeds with fruit trees offer a myriad of benefits and an efficient system for restoring the soil C without compromising the food and livelihood security of the rural populace in semiarid regions.     

Deep soil flipping increases carbon stocks of New Zealand grasslands

Sequestration of soil organic carbon (SOC) has been recognized as an opportunity to off‐set global carbon dioxide (CO₂) emissions. Flipping (full inversion to 1–3 m) is a practice used on New Zealand's South Island West Coast to eliminate water‐logging in highly podzolized sandy soils. Flipping results in burial of SOC formed in surface soil horizons into the subsoil and the transfer of subsoil material low in SOC to the “new” topsoil. The aims of this study were to quantify changes in the storage and stability of SOC over a 20‐year period following flipping of high‐productive pasture grassland. Topsoils (0–30 cm) from sites representing a chronosequence of flipping (3–20 years old) were sampled (2005/07) and re‐sampled (2017) to assess changes in topsoil carbon stocks. Deeper samples (30–150 cm) were also collected (2017) to evaluate the changes in stocks of SOC previously buried by flipping. Density fractionation was used to determine SOC stability in recent and buried topsoils. Total SOC stocks (0–150 cm) increased significantly by 69 ± 15% (179 ± 40 Mg SOC ha^‐1) over 20 years following flipping. Topsoil burial caused a one‐time sequestration of 160 ± 14 Mg SOC ha^‐1 (30–150 cm). The top 0–30 cm accumulated 3.6 Mg SOC ha^‐1 year^‐1. The chronosequence and re‐sampling revealed SOC accumulation rates of 1.2–1.8 Mg SOC ha^‐1 year^‐1 in the new surface soil (0–15 cm) and a SOC deficit of 36 ± 5% after 20 years. Flipped subsoils contained up to 32% labile SOC (compared to <1% in un‐flipped subsoils) thus buried SOC was preserved. This study confirms that burial of SOC and the exposure of SOC depleted subsoil results in an overall increase of SOC stocks of the whole soil profile and long‐term SOC preservation.     

No changes in soil organic carbon and nitrogen following long-term prescribed burning and livestock exclusion in the Sudan-savanna woodlands of Burkina Faso

Fire and overgrazing reduce aboveground biomass, leading to land degradation and potential impacts on soil organic carbon (SOC) and total nitrogen (TN) dynamics. However, empirical data are lacking on how prescribed burning and livestock exclusion impact SOC in the long-term. Here we analyse the effects of 19 years of prescribed annual burning and livestock exclusion on tree density, SOC and TN concentrations in the Sudanian savanna ecoregion at two sites (Tiogo and Laba) in Burkina Faso. Results revealed that neither livestock exclusion nor prescribed burning had significant impact on SOC and TN concentrations. The results at both sites indicate that 19 years of livestock and fire exclusion did not result in a significant increase in tree density compared to grazing and annual prescribed burning. The overall mean (± SEM) of SOC stocks in the 0–50 cm depth increment in the unburnt (53.5 ± 4.7 Mg C ha⁻¹) and annually burnt (56.4 ± 4.3 Mg C ha⁻¹) plots at Tiogo were not statistically different. Similarly, at Laba there was no significant difference between the corresponding figures in the unburnt (37.9 ± 2.6 Mg ha⁻¹) and in the annually burnt plots (38.6 ± 1.9 Mg ha⁻¹). Increases in belowground inputs from root turnover may have countered changes in aboveground biomass, resulting in no net change in SOC and TN. We conclude that, contrary to our expectation and current policy recommendations, restricting burning or grazing did not result in increase in SOC stocks in this dry savanna ecosystem.     

Quantifying the effects of switchgrass (Panicum virgatum) on deep organic C stocks using natural abundance 14C in three marginal soils

Perennial bioenergy crops have been shown to increase soil organic carbon (SOC) stocks, potentially offsetting anthropogenic C emissions. The effects of perennial bioenergy crops on SOC are typically assessed at shallow depths (<30 cm), but the deep root systems of these crops may also have substantial effects on SOC stocks at greater depths. We hypothesized that deep (>30 cm) SOC stocks would be greater under bioenergy crops relative to stocks under shallow‐rooted conventional crop cover. To test this, we sampled soils to between 1‐ and 3‐m depth at three sites in Oklahoma with 10‐ to 20‐year‐old switchgrass (Panicum virgatum) stands, and collected paired samples from nearby fields cultivated with shallow rooted annual crops. We measured root biomass, total organic C, ¹⁴C, ¹³C, and other soil properties in three replicate soil cores in each field and used a mixing model to estimate the proportion of recently fixed C under switchgrass based on ¹⁴C. The subsoil C stock under switchgrass (defined over 500–1500 kg/m² equivalent soil mass, approximately 30–100 cm depth) exceeded the subsoil stock in neighboring fields by 1.5 kg C/m² at a sandy loam site, 0.6 kg C/m² at a site with loam soils, and showed no significant difference at a third site with clay soils. Using the mixing model, we estimated that additional SOC introduced after switchgrass cultivation comprised 31% of the subsoil C stock at the sandy loam site, 22% at the loam site, and 0% at the clay site. These results suggest that switchgrass can contribute significantly to subsoil organic C—but also indicated that this effect varies across sites. Our analysis shows that agricultural strategies that emphasize deep‐rooted grass cultivars can increase soil C relative to conventional crops while expanding energy biomass production on marginal lands.     

Measured and modelled effect of land‐use change from temperate grassland to Miscanthus on soil carbon stocks after 12 years

Soil organic carbon (SOC) is an important carbon pool susceptible to land‐use change (LUC). There are concerns that converting grasslands into the C₄ bioenergy crop Miscanthus (to meet demands for renewable energy) could negatively impact SOC, resulting in reductions of greenhouse gas mitigation benefits gained from using Miscanthus as a fuel. This work addresses these concerns by sampling soils (0–30 cm) from a site 12 years (T₁₂) after conversion from marginal agricultural grassland into Miscanthus x giganteus and four other novel Miscanthus hybrids. Soil samples were analysed for changes in below‐ground biomass, SOC and Miscanthus contribution to SOC (using a ¹³C natural abundance approach). Findings are compared to ECOSSE soil carbon model results (run for a LUC from grassland to Miscanthus scenario and continued grassland counterfactual), and wider implications are considered in the context of life cycle assessments based on the heating value of the dry matter (DM) feedstock. The mean T₁₂ SOC stock at the site was 8 (±1 standard error) Mg C/ha lower than baseline time zero stocks (T₀), with assessment of the five individual hybrids showing that while all had lower SOC stock than at T₀ the difference was only significant for a single hybrid. Over the longer term, new Miscanthus C₄ carbon replaces pre‐existing C₃ carbon, though not at a high enough rate to completely offset losses by the end of year 12. At the end of simulated crop lifetime (15 years), the difference in SOC stocks between the two scenarios was 4 Mg C/ha (5 g CO₂‐eq/MJ). Including modelled LUC‐induced SOC loss, along with carbon costs relating to soil nitrous oxide emissions, doubled the greenhouse gas intensity of Miscanthus to give a total global warming potential of 10 g CO₂‐eq/MJ (180 kg CO₂‐eq/Mg DM).     

Soil Carbon Storage by Switchgrass Grown for Bioenergy

Life-cycle assessments (LCAs) of switchgrass (Panicum virgatum L.) grown for bioenergy production require data on soil organic carbon (SOC) change and harvested C yields to accurately estimate net greenhouse gas (GHG) emissions. To date, nearly all information on SOC change under switchgrass has been based on modeled assumptions or small plot research, both of which do not take into account spatial variability within or across sites for an agro-ecoregion. To address this need, we measured change in SOC and harvested C yield for switchgrass fields on ten farms in the central and northern Great Plains, USA (930 km latitudinal range). Change in SOC was determined by collecting multiple soil samples in transects across the fields prior to planting switchgrass and again 5 years later after switchgrass had been grown and managed as a bioenergy crop. Harvested aboveground C averaged 2.5?±?0.7 Mg C ha^?1 over the 5 year study. Across sites, SOC increased significantly at 0–30 cm (P?=?0.03) and 0–120 cm (P?=?0.07), with accrual rates of 1.1 and 2.9 Mg C ha^?1 year^?1 (4.0 and 10.6 Mg CO₂ ha^?1 year^?1), respectively. Change in SOC across sites varied considerably, however, ranging from ?0.6 to 4.3 Mg C ha^?1 year^?1 for the 0–30 cm depth. Such variation in SOC change must be taken into consideration in LCAs. Net GHG emissions from bioenergy crops vary in space and time. Such variation, coupled with an increased reliance on agriculture for energy production, underscores the need for long-term environmental monitoring sites in major agro-ecoregions.     

No general soil carbon sequestration under Central European short rotation coppices

Wood from short rotation coppices (SRCs) is discussed as bioenergy feedstock with good climate mitigation potential inter alia because soil organic carbon (SOC) might be sequestered by a land-use change (LUC) from cropland to SRC. To test if SOC is generally enhanced by SRC over the long term, we selected the oldest Central European SRC plantations for this study. Following the paired plot approach soils of the 21 SRCs were sampled to 80 cm depth and SOC stocks, C/N ratios, pH and bulk densities were compared to those of adjacent croplands or grasslands. There was no general trend to SOC stock change by SRC establishment on cropland or grassland, but differences were very site specific. The depth distribution of SOC did change. Compared to cropland soils, the SOC density in 0–10 cm was significantly higher under SRC (17 ± 2 in cropland and 21 ± 2 kg C m⁻³ in SRC). Under SRC established on grassland SOC density in 0–10 cm was significantly lower than under grassland. The change rates of total SOC stocks by LUC from cropland to SRC ranged from −1.3 to 1.4 Mg C ha⁻¹ yr⁻¹ and −0.6 Mg C ha⁻¹ yr⁻¹ to +0.1 Mg C ha⁻¹ yr⁻¹ for LUC from grassland to SRC, respectively. The accumulation of organic carbon in the litter layer was low (0.14 ± 0.08 Mg C ha⁻¹ yr⁻¹). SOC stocks of both cropland and SRC soils were correlated with the clay content. No correlation could be detected between SOC stock change and soil texture or other abiotic factors. In summary, we found no evidence of any general SOC stock change when cropland is converted to SRC and the identification of the factors determining whether carbon may be sequestered under SRC remains a major challenge.     

Accumulation of soil organic carbon after cropland conversion to short‐rotation willow and poplar

The demand for bioenergy has increased the interest in short‐rotation woody crops (SRWCs) in temperate zones. With increased litter input and ceased annual soil cultivation, SRWC plantations may become soil carbon sinks for climate change mitigation. A chronosequence of 26 paired plots was used to study the potential for increasing soil organic carbon (SOC) under SRWC willow and poplar after conversion from cropland (CR) on well‐drained soils. We estimated SOC stocks in SRWC stands and adjacent CR and related the difference to time since conversion, energy crop species, SOC stock of the adjacent CR (proxy for initial SOC of SRWC) and the fine soil percentage (<63 μm) (FS). Soil cores to 40 cm depth were sampled and separated by layers of fixed depths (0–5, 5–10, 10–15, 15–25 and 25–40 cm). Additionally, soils were sampled from soil pits by genetic horizons to 100 cm depth. Comparisons of SOC stocks by equivalent soil masses showed that mean SOC stocks in SRWC were 1.7 times higher than those of CR in the top 5 cm of the soil (P < 0.001). The differences between SRWC and CR remained significant for the plough layer (0–25 cm) by a factor of 1.2 (P = 0.003), while no changes were detectable for the 0–40 cm (P = 0.32), or for the entire 0–100 cm soil layer (P = 0.29). The SOC stock ratio, that is the ratio of SOC stock in SRWC relative to CR, did not change significantly with time since conversion, although there was a tendency to an increase over time for the top 40 cm (P = 0.09). The SOC stock ratio was negatively correlated to SOC in CR and FS percentage, but there was no significant difference between willow and poplar at any depth. Our results suggest that SOC stocks in the plough layer increase after conversion to SRWC.     

Soil Carbon Sequestration by Switchgrass and No-Till Maize Grown for Bioenergy

Net benefits of bioenergy crops, including maize and perennial grasses such as switchgrass, are a function of several factors including the soil organic carbon (SOC) sequestered by these crops. Life cycle assessments (LCA) for bioenergy crops have been conducted using models in which SOC information is usually from the top 30 to 40?cm. Information on the effects of crop management practices on SOC has been limited so LCA models have largely not included any management practice effects. In the first 9?years of a long-term C sequestration study in eastern Nebraska, USA, switchgrass and maize with best management practices had average annual increases in SOC per hectare that exceed 2?Mg?C?year^?1 (7.3?Mg?CO₂?year^?1) for the 0 to 150 soil depth. For both switchgrass and maize, over 50?% of the increase in SOC was below the 30?cm depth. SOC sequestration by switchgrass was twofold to fourfold greater than that used in models to date which also assumed no SOC sequestration by maize. The results indicate that N fertilizer rates and harvest management regimes can affect the magnitude of SOC sequestration. The use of uniform soil C effects for bioenergy crops from sampling depths of 30 to 40?cm across agro-ecoregions for large scale LCA is questionable.     

Soil carbon stock impacts following reversion of Miscanthus × giganteus and short rotation coppice willow commercial plantations into arable cropping

There are posited links between the establishment of perennial bioenergy, such as short rotation coppice (SRC) willow and Miscanthus × giganteus, on low carbon soils and enhanced soil C sequestration. Sequestration provides additional climate mitigation, however, few studies have explored impacts on soil C stocks of bioenergy crop removal; thus, the permanence of any sequestered C is unclear. This uncertainty has led some authors to question the handling of soil C stocks with carbon accounting, for example, through life cycle assessments. Here, we provide additional data for this debate, reporting on the soil C impacts of the reversion (removal and return) to arable cropping of commercial SRC willow and Miscanthus across four sites in the UK, two for each bioenergy crop, with eight reversions nested within these sites. Using a paired‐site approach, soil C stocks (0–1 m) were compared between 3 and 7 years after bioenergy crop removal. Impacts on soil C stocks varied, ranging from an increase of 70.16 ± 10.81 Mg C/ha 7 years after reversion of SRC willow to a decrease of 33.38 ± 5.33 Mg C/ha 3 years after reversion of Miscanthus compared to paired arable land. The implications for carbon accounting will depend on the method used to allocate this stock change between current and past land use. However, with published life cycle assessment values for the lifetime C reduction provided by these crops ranging from 29.50 to 138.55 Mg C/ha, the magnitude of these changes in stock are significant. We discuss the potential underlying mechanisms driving variability in soil C stock change, including the age of bioenergy crop at removal, removal methods, and differences in the recalcitrant of the crop residues, and highlight the need to design management methods to limit negative outcomes.     

Impact of woody encroachment on soil organic carbon storage in the Lopé National Park,Gabon

This study quantifies changes in soil organic carbon (SOC) stock as a result of woody encroachment on savannas. Changes in SOC stocks occur below 30 cm depth, indicating the subsoil as the principal compartment contributing to SOC sequestration, and suggesting the need to consider the entire profile (0–100 cm) to thoroughly assess the effect of woody encroachment on SOC stocks.     

Temporal dynamics of soil organic carbon after land‐use change in the temperate zone – carbon response functions as a model approach

Land‐use change (LUC) is a major driving factor for the balance of soil organic carbon (SOC) stocks and the global carbon cycle. The temporal dynamic of SOC after LUC is especially important in temperate systems with a long reaction time. On the basis of 95 compiled studies covering 322 sites in the temperate zone, carbon response functions (CRFs) were derived to model the temporal dynamic of SOC after five different LUC types (mean soil depth of 30±6 cm). Grassland establishment caused a long lasting carbon sink with a relative stock change of 128±23% and afforestation on former cropland a sink of 116±54%, 100 years after LUC (mean±95% confidence interval). No new equilibrium was reached within 120 years. In contrast, there was no SOC sink following afforestation of grasslands and 75% of all observations showed SOC losses, even after 100 years. Only in the forest floor, there was carbon accumulation of 0.38±0.04 Mg ha^?1 yr^?1 in afforestations adding up to 38±4 Mg ha^?1 labile carbon after 100 years. Carbon loss after deforestation (?32±20%) and grassland conversion to cropland (?36±5%), was rapid with a new SOC equilibrium being reached after 23 and 17 years, respectively. The change rate of SOC increased with temperature and precipitation but decreased with soil depth and clay content. Subsoil SOC changes followed the trend of the topsoil SOC changes but were smaller (25±5% of the total SOC changes) and with a high uncertainty due to a limited number of datasets. As a simple and robust model approach, the developed CRFs provide an easily applicable tool to estimate SOC stock changes after LUC to improve greenhouse gas reporting in the framework of UNFCCC.