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1.
Tomato is the most important vegetable crop in Spain. The mirid bug Nesidiocoris tenuis (Reuter) commonly appears in large numbers in protected and open-air tomato crops where little or no broad-spectrum insecticides
are used. Nesidiocoris tenuis is known to be a predator of whiteflies, thrips and several other pest species. However, it is also considered a pest because
it can feed on tomato plants, causing necrotic rings on stems and flowers and punctures in fruits. Our objectives were to
evaluate predation by N. tenuis on sweetpotato whitefly Bemisia tabaci Gennadius under greenhouse conditions and establish its relationship to N. tenuis feeding on tomato. Two different release rates of N. tenuis were compared with an untreated control (0, 1 and 4 N. tenuis plant−1) in cages of 8 m2. Significant reductions of greater than 90% of the whitefly population and correspondingly high numbers of N. tenuis were observed with both release rates. Regression analysis showed that necrotic rings on foliage caused by N. tenuis were best explained by the ratio of B. tabaci nymphs:N. tenuis as predicted by the equation y = 15.086x − 0.6359.
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Alberto UrbanejaEmail: |
2.
Lucien N’Guessan Kouassi Katsuo Tsuda Chie Goto Shigeyuki Mukawa Yositaka Sakamaki Masayuki Nakamura 《BioControl》2009,54(4):537-548
We compared the infectivity of two nucleopolyhedroviruses (NPVs), MyseNPV G isolated from Mythimna separata (Walker) (Lepidoptera: Noctuidae) and SpltNPV S isolated from Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). MyseNPV G was more pathogenic against M. separata than against S. litura. Although SpltNPV S was more pathogenic than MyseNPV G against S. litura, it did not infect M. separata. Restriction endonuclease (REN) analysis of viral genomic DNA revealed that the two NPVs have quite different REN profiles.
Based on nucleotide sequences of the coding regions of polyhedrin, lef-8 and lef-9, SpltNPV S was closely related to other SpltNPV isolates, whereas MyseNPV G appeared to belong to the Mamestra NPV group, and was distinct from a Chinese isolate of Leucania (=Mythimna) separata NPV. The potential of MyseNPV G and SpltNPV S to control pest insects is discussed.
相似文献
Katsuo Tsuda (Corresponding author)Email: |
3.
Antibodies are very often used as specific cell and/or tissue markers. An example of this is anti-horseradish peroxidase (HRP),
an antibody raised against a plant glycoprotein, which was shown some twenty-five years ago to specifically stain neural tissue
in an animal, Drosophila melanogaster. This peculiar finding was later expanded to other invertebrate species including Caenorhabditis elegans, which were also shown to bear anti-HRP epitopes. Initial experiments indicated that the epitopes recognised by anti-HRP
in invertebrates are of carbohydrate nature. Indeed, more recent experiments have characterised relevant core α1-3-fucosylated
N-glycan structures that act as epitopes in various model and parasitic organisms. Moreover, a number of enzymes required
for the synthesis of such structures have been identified. Over the years, medically-relevant roles of these structures have
become apparent as regards allergenicity and immunoregulation. Although major advances have been made in understanding of
the underlying mechanisms and structures related to the anti-HRP epitope, the in vivo role of the relevant epitopes in neural and other tissues is yet to be resolved. Current understanding of the anti-HRP epitopes
synthesis and their relevance is discussed and elaborated.
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Katharina PaschingerEmail: |
4.
Karen E. Chambers Ryan McDaniell Jeremy D. Raincrow Maya Deshmukh Peter F. Stadler 《Theorie in den Biowissenschaften》2009,128(2):109-120
Large-scale—even genome-wide—duplications have repeatedly been invoked as an explanation for major radiations. Teleosts, the
most species-rich vertebrate clade, underwent a “fish-specific genome duplication” (FSGD) that is shared by most ray-finned
fish lineages. We investigate here the Hox complement of the goldeye (Hiodon alosoides), a representative of Osteoglossomorpha, the most basal teleostean clade. An extensive PCR survey reveals that goldeye has
at least eight Hox clusters, indicating a duplicated genome compared to basal actinopterygians. The possession of duplicated Hox clusters is uncoupled to species richness. The Hox system of the goldeye is substantially different from that of other teleost lineages, having retained several duplicates
of Hox genes for which crown teleosts have lost at least one copy. A detailed analysis of the PCR fragments as well as full length
sequences of two HoxA13 paralogs, and HoxA10 and HoxC4 genes places the duplication event close in time to the divergence of Osteoglossomorpha and crown teleosts. The data are
consistent with—but do not conclusively prove—that Osteoglossomorpha shares the FSGD.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
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Chi-hua ChiuEmail: |
5.
6.
Mycena aphanes and Mycena exilis, belonging to section Filipedes and section Polyadelphia, are proposed as new species. They are described and illustrated and compared to other species in the two sections. Mycena aphanes is a strikingly characteristic Mycena with fulvous or yellowish brown colours of both pileus and stipe. Mycena exilis is characterized by its occurrence on fallen Salix leaves, a pale brown pileus, occasionally with a pale pink tinge, narrowly adnate lamellae, four-spored basidia, and the
presence of clamp connections.
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Gro GuldenEmail: |
7.
The interconnection network is one of the key architectural components in any parallel computer. The distribution of the traffic
injected into the network is among the factors that greatly influences network performance. The uniform traffic pattern has
been adopted in many existing network performance evaluation studies due to the tractability of the resulting analytical modelling
approach. However, many real applications exhibit non-uniform traffic patterns such as hot-spot traffic. K-ary n-cubes have been the mostly widely used in the implementation of practical parallel systems. Extensive research studies have
been conducted on the performance modelling and evaluation of these networks. Nonetheless, most of these studies have been
confined to uniform traffic distributions and have been based on software simulation. The present paper proposes a new stochastic
model to predict message latency in k-ary
n-cubes with deterministic routing in the presence of hot-spot traffic. The model has been validated through simulation experiments
and has shown a close agreement with simulation results.
相似文献
Geyong MinEmail: |
8.
Kalina Bermúdez-Torres Jorge Martínez Herrera Rodolfo Figueroa Brito Michael Wink Luc Legal 《BioControl》2009,54(3):459-466
Bitter lupins (Lupinus spp.) are not used as a protein source because of their toxicity. However, they may have alternative uses as potential sources
of natural insecticides. Quinolizidine alkaloids (QA) of three Mexican Lupinus species (Fabaceae): L. montanus (HBK),
L. stipulatus (Agardh) and L. aschenbornii (Schauer), were analyzed by capillary Gas Chromatography-Mass Spectrometry. Sparteine was found in high amounts in both L. montanus and L. aschenbornii while the major alkaloids in L. stipulatus extract were aphylline and an epiaphylline-like compound. Alkaloid extracts were tested for their insecticidal activity using
larvae of the Fall Armyworm, Spodoptera frugiperda (Smith); (Lepidoptera, Noctuidae) as a model pest. We compared LD50 values and mean weight of caterpillars fed with alkaloid extracts of the three species studied with those of sparteine, a
widespread QA found in various lupin species. Extracts of L. montanus and L. aschenbornii were found to be as effective as sparteine and extracts L. stipulatus were found to be the most toxic against the larvae of S. frugiperda. This suggests that the various QA act differently on caterpillars, and could be used to control Spodoptera populations.
相似文献
Kalina Bermúdez-TorresEmail: |
9.
A total of eight strains of bacteria were isolated from the root nodule of Vicia faba on the selective media of Rhizobium. Two of these strains produced phenotypically distinct mucoid colonies (one slow growing and the other fast growing) and
were examined using a polyphasic approach for taxonomic identification. The two strains (MTCC 7405 and MTCC 7406) turned out
to be new strains of biovar 1 Agrobacterium rather than Rhizobium, as they showed growth on alkaline medium as well as on 2% NaCl and neither catabolized lactose as the carbon source nor
oxidized Tween-80. The distinctness between the two strains was marked with respect to their growth on dextrose and the production
of lysine dihydrolase, ornithine decarboxylase and DNA G + C content. 16S rDNA sequencing and their comparison with the 16S
rDNA sequences of previously described agrobacteria as well as rhizobia strains confirmed the novelty of the two strains.
Both of the strains clustered with strains of Agrobacterium tumefaciens in the 16S rDNA-based phylogenetic tree. The phenotypic and biochemical properties of the two strains differed from those
of the recognized biovar of A. tumefaciens. It is proposed that the strains MTCC 7405 and MTCC 7406 be classified as novel biovar of the species A. tumefaciens (Type strains MTCC 7405 = DQ383275 and MTCC 7406 = DQ383276).
相似文献
Bhupendra N. TiwaryEmail: |
10.
11.
12.
The recent sequencing of the first tree genome, that of the black cottonwood (Populus trichocarpa), opens a new chapter in tree functional genomics. While the completion of the genome is a milestone, mobilizing this significant
resource for better understanding the growth and development of woody perennials will be an even greater undertaking in the
years to come. In other model organisms, a critical tool for high-throughput analysis of gene function has been the generation
of large mutagenized populations. Some mutagenesis technologies and approaches cannot be applied to trees because of their
typically outcrossing breeding systems, high heterozygosity, large body size, and delayed flowering. In contrast, gene-tagging
approaches that use insertional mutagenesis to create dominant phenotypes are ideally suited for trees and, especially, Populus. Both activation tagging and enhancer trap programs have been successful in identifying new genes important to tree development.
The generation of genome-wide insertional mutant populations, which provide direct functional links between genes and phenotypes,
should help to integrate in silico analyses of gene and protein expression, association studies of natural genetic polymorphism, and phenotypic analyses of
adaptation and development.
相似文献
Victor BusovEmail: |
13.
14.
Nasser Said Mandour 《BioControl》2009,54(1):93-102
Toxicity of spinosad to immature stages of Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) and its effect on the reproduction and survival of adult stages after direct spray and
ingestion treatments were evaluated. Spinosad was harmless to C. carnea eggs and pupae irrespective of concentrations or method of treatments. Direct spray of spinosad to first instar caused significant
reduction in rate of pupation and L1-adult survival, but did not affect the rate of adult emergence. Third-instar bioassay
revealed significant difference in L3-adult survival. When C. carnea first instars were fed upon spinosad-treated-Brevicoryne
brassicae (L.), significant differences in investigated parameters were not observed among different treatments. The weight of third
instar and pupae were comparable regardless of tested spinosad concentrations. Also, feeding on spinosad treated-B. brassicae had no negative impact on fecundity and fertility. Furthermore, ingestion of spinosad contaminated-B. brassicae significantly prolonged larval and larval + pupal periods. The larval feeding capacity of C. carnea did not differ significantly between larvae fed on spinosad-treated and untreated aphids with the exception of first instar.
When C. carnea adults were allowed to oviposit on spinosad treated-substrate, total number of eggs laid, percent of eggs laid on the treated
substrate and egg hatching did not differ from those of control. However, when adults were fed on spinosad-treated artificial
diet, negative effects on adult survival and fecundity were observed.
相似文献
Nasser Said MandourEmail: Email: |
15.
The haulout patterns of five adult and two juvenile crabeater seals (Lobodon carcinophaga) were documented from February to December 1995 in three areas of the Antarctic. The longest haulout event was 19.2 h (mean=7.9, median=7.8); the longest in-water event was 85.8 h (mean=15.1, median=14.2). In no month were more than 76% of the adult seals in our sample hauled-out simultaneously. Multivariate repeated measures analysis of variance tests revealed no difference between males and females in the daily timing of haulout (P>0.05) between March and August (the only months with an adequate sample); in contrast, haulout timing differed between juvenile and adult seals (P<0.05) except in May. Juveniles spent about twice as much time hauled-out on the ice as adults (38% vs 20%, respectively; P<0.05). Predator avoidance by juveniles is hypothesized to be a possible cause for these differences. In light of these haulout patterns, population surveys of crabeater seals conducted in February, and as close as possible to local solar noon, can be expected to encounter the highest proportion of seals hauled-out on ice.
相似文献
John L. BengtsonEmail: Phone: +1-206-526-4016Fax: +1-206-526-6615 |
16.
Alison M. Strange Geoffrey H. Griffiths Sophie Hine Kim Young Graham J. Holloway 《Journal of Insect Conservation》2007,11(3):241-249
The Small Red Damselfly (Ceriagrion tenellum) (De Villiers) (Odonata: Coenagrionidae: Ceriagrion) is classed as vulnerable (Shirt, British Red Data Book, Nature Conservancy Council, Peterborough, UK, 1987) throughout the
UK, and is included in certain Local Biodiversity Action Plans (LBAPs) in the south. A large proportion of any Biodiversity
Action Plan is concerned with the requirement of conservation and management programmes. In order to guide them, information
about the habitat preferences of the species concerned is vital. Detailed habitat information was collected to include a variety
of physical parameters particularly vegetation, both in-channel and bankside. The species was found to be primarily associated
with in-channel emergent broad-leaved plants, bankside grasses and rushes, and shallow, narrow channels with dark organic
substrate. The consequences of these findings are discussed in relation to the conservation and management of C. tenellum.
相似文献
Alison M. StrangeEmail: |
17.
Richard J. Robins Roland Molinié Renata A. Kwiecień Piotr Paneth Jacques Lebreton Trixie A. Bartholomeusz Albrecht Roscher Birgit Dräger Anna-Carolin Meier François Mesnard 《Phytochemistry Reviews》2007,6(1):51-63
The reaction of N-demethylation plays an important role in the degradation of some alkaloids in a number of organisms. This review presents
how our understanding of the N-demethylation of nicotine in plants has been improved through studies in cell cultures of Nicotiana plumbaginifolia and N. glutinosa using a variety of isotopic techniques. The overall aim is to understand how metabolism recycles the alkaloid skeleton, both
in terms of the metabolic route(s) exploited and the reaction mechanisms of the enzymes involved. The former has been approached
using high-resolution 2-dimensional NMR and GC-MS methods; the latter by determining kinetic isotope effects and modelling
the potential reaction steps. It appears that the mechanism for nicotine demethylation in plants is similar to but has significant
differences from that described for mammals and Pseudomonas bacteria. These differences are discussed.
相似文献
Richard J. RobinsEmail: |
18.
Methanogens growing on C-1 substrates synthesize 2-carbon acetyl groups in the form of acetyl-CoA for carbon assimilation
using the multienzyme complex acetyl-CoA decarbonylase/synthase (ACDS) which contains five different subunits encoded within
an operon. In species growing on acetate ACDS also functions to cleave the acetate C-C bond for energy production by methanogenesis.
A number of species of Methanosarcina that are capable of growth on either C-1 compounds or acetate contain two separate ACDS operons, and questions have been
raised about whether or not these operons play separate roles in acetate synthesis and cleavage. Methanosarcina thermophila genomic DNA was analyzed for the presence of two ACDS operons by PCR amplifications with different primer pairs, restriction
enzyme analyses, DNA sequencing and Southern blot analyses. A single ACDS operon was identified and characterized, with no
evidence for more than one. MALDI mass spectrometric analyses were carried out on ACDS preparations from methanol- and acetate-grown
cells. Peptide fragmentation patterns showed that the same ACDS subunits were present regardless of growth conditions. The
evidence indicates that a single form of ACDS is used both for acetate cleavage during growth on acetate and for acetate synthesis
during growth on C-1 substrates.
Electronic Supplementary Material Supplementary material is available for this article at
相似文献
David A. Grahame (Corresponding author)Email: |
Edward DeMoll (Corresponding author)Email: |
19.
Nagayasu Nakanishi Volker Hartenstein David K. Jacobs 《Development genes and evolution》2009,219(6):301-317
We examined the development of the nervous system in the rhopalium, a medusa-specific sensory structure, in Aurelia sp.1 (Cnidaria, Scyphozoa) using confocal microscopy. The rhopalial nervous system appears primarily ectodermal and contains
neurons immunoreactive to antibodies against tyrosinated tubulin, taurine, GLWamide, and FMRFamide. The rhopalial nervous
system develops in an ordered manner: the presumptive gravity-sensing organ, consisting of the lithocyst and the touch plate,
differentiates first; the “marginal center,” which controls swimming activity, second; and finally, the ocelli, the presumptive
photoreceptors. At least seven bilaterally arranged neuronal clusters consisting of sensory and ganglion cells and their neuronal
processes became evident in the rhopalium during metamorphosis to the medusa stage. Our analysis provides an anatomical framework
for future gene expression and experimental studies of development and functions of scyphozoan rhopalia.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
相似文献
David K. Jacobs (Corresponding author)Email: |
20.
Geoffrey R. Dixon 《Journal of Plant Growth Regulation》2009,28(3):212-228
Plasmodiophora brassicae Wor. is viewed in this article from the standpoint of a highly evolved and successful organism, well fitted for the ecological
niche that it occupies. Physical, chemical, and biological components of the soil environment are discussed in relation to
their effects on the survival, growth, and reproduction of this microbe. It is evident that P. brassicae is well equipped by virtue of its robust resting spores for survival through many seasonal cycles. Germination is probably
triggered as a result of signals initiated by root exudates. The resultant motile zoospore moves rapidly to the root hair
surface and penetration and colonization follow. The short period between germination and penetration is one of greatest vulnerability
for P. brassicae. In this phase survival is affected at the very least by soil texture and structure; its moisture; pH; calcium, boron, and
nitrogen content; and the presence of active microbial antagonists. These factors influence the inoculum potential (sensu Garrett, 1956) and its viability and invasive capacity. There is evidence that these effects may also influence differentially
the survival of some physiologic races of P. brassicae. Considering the interaction of P. brassicae with the soil environment from the perspective of its biological fitness is an unusual approach; most authors consider only
the opportunities to destroy this organism. The approach adopted here is borne of several decades spent studying P. brassicae and the respect that has been engendered for it as a biological entity. This review stops at the point of penetration, although
some of the implications of the environment for successful colonization are included because they form a continuum. Interactions
with the molecular and biochemical cellular environment are considered in other sections in this special edition.
相似文献
Geoffrey R. DixonEmail: |