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1.
Schwander F Eibach R Fechter I Hausmann L Zyprian E Töpfer R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(1):163-176
A population derived from a cross between grapevine breeding strain Gf.Ga-52-42 and cultivar ‘Solaris’ consisting of 265 F1-individuals
was genetically mapped using SSR markers and screened for downy mildew resistance. Quantitative trait locus (QTL) analysis
revealed two strong QTLs on linkage groups (LGs) 18 and 09. The locus on LG 18 was found to be identical with the previously
described locus Rpv3 and is transmitted by Gf.Ga-52-42. ‘Solaris’ transmitted the resistance-related locus on LG 09 explaining up to 50% of the
phenotypic variation in the population. This downy mildew resistance locus is named Rpv10 for resistance to Plasmopara viticola. Rpv10 was initially introgressed from Vitis amurensis, a wild species of the Asian Vitis gene pool. The one-LOD supported confidence interval of the QTL spans a section of 2.1 centi Morgan (cM) corresponding to
314 kb in the reference genome PN40024 (12x). Eight resistance gene analogues (RGAs) of the NBS–LRR type and additional resistance-linked
genes are located in this region of PN40024. The F1 sub-population which contains the Rpv3 as well as the Rpv10 locus showed a significantly higher degree of resistance, indicating additive effects by pyramiding of resistance loci. Possibilities
for using the resistance locus Rpv10 in a grapevine breeding programme are discussed. Furthermore, the marker data revealed ‘Severnyi’ × ‘Muscat Ottonel’ as the
true parentage for the male parent of ‘Solaris’. 相似文献
2.
Different hypotheses on the genetic control of the resistance to the plum pox virus (PPV) have been reported in apricot, but
there was a lack of agreement about the number of loci involved. In recent years, apricot genetic maps have been constructed
from progenies derived from ‘Stark Early Orange’ or ‘Goldrich’, two main sources of resistance, three of these including the
mapping of the PPV resistance loci. As the location of the locus was not precisely established, we mapped the PPV resistance
loci using interval mapping (IM), composite interval mapping (CIM), and the Kruskal–Wallis non-parametric test in the F1 progeny derived from a cross between the susceptible cv. ‘Polonais’ and ’Stark Early Orange’. Four genomic regions were identified
as being involved in PPV resistance. One of these mapped to the upper region of linkage group 1 of ‘Stark Early Orange’, and
accounted for 56% of the phenotypic variation. Its location was similar to the one previously identified in ‘Goldrich’ and
Prunus davidiana. In addition, a gene strongly associated to these major quantitative trait loci (QTL) was found to be related to PPV infection.
Two putative QTLs were detected on linkage groups 3 of ‘Polonais’ and 5 of both ‘Polonais’ and ‘Stark Early Orange’ with both
parametric and non-parametric methods at logarithm of odds (LOD) scores slightly above the detection threshold. The last QTL
was only detected in the early stage of the infection. PPV resistance is, thus, controlled by a major dominant factor located
on linkage group 1. The hypothesis of recessive factors with lower effect is discussed. 相似文献
3.
Junke Zhang Ludger Hausmann Rudolf Eibach Leocir J. Welter Reinhard T?pfer Eva M. Zyprian 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(6):1039-1051
Grapevine rootstock cultivar ‘B?rner’ is a hybrid of Vitis riparia and Vitis cinerea Arnold that shows high resistance to phylloxera (Daktulosphaira vitifoliae Fitch). To localize the determinants of phylloxera root resistance, the susceptible grapevine V3125 (Vitis vinifera ‘Schiava grossa’ × ‘Riesling’) was crossed to ‘B?rner’. Genetic framework maps were built from the progeny. 235 microsatellite
markers were placed on the integrated parental map. They cover 1,155.98 cM on 19 linkage groups with an average marker distance
of 4.8 cM. Phylloxera resistance was scored by counting nodosities after inoculation of the root system. Progeny plants were
triplicated and experimentally infected in 2 years. A scan of the genetic maps indicated a quantitative trait locus on linkage
group 13. This region was targeted by six microsatellite-type markers newly developed from the V. vinifera model genome sequence. Two of these appear closely linked to the trait, and can be useful for marker-assisted breeding. 相似文献
4.
Accumulation of additive effects generates a strong photoperiod sensitivity in the extremely late-heading rice cultivar ‘Nona Bokra’ 总被引:1,自引:0,他引:1
Uga Y Nonoue Y Liang ZW Lin HX Yamamoto S Yamanouchi U Yano M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(8):1457-1466
Many rice cultivars that originated from lower-latitude regions exhibit a strong photoperiod sensitivity (PS) and show extremely
late heading under long-day conditions. Under natural day-length conditions during the cropping season in Japan, the indica rice cultivar ‘Nona Bokra’ from India showed extremely late heading (202 days to heading) compared to the japonica cultivar ‘Koshihikari’ (105 days), from Japan. To elucidate the genetic factors associated with such extremely late heading,
we performed quantitative trait locus (QTL) analyses of heading date using an F2 population and seven advanced backcross progeny (one BC1F2 and six BC2F2) derived from a cross between ‘Nona Bokra’ and ‘Koshihikari’. The analyses revealed 12 QTLs on seven chromosomes. The ‘Nona
Bokra’ alleles of all QTLs contributed to an increase in heading date. Digenic interactions were rarely observed between QTLs.
Based on the genetic parameters of the QTLs, such as additive effects and percentage of phenotypic variance explained, these
12 QTLs are likely generate a large proportion of the phenotypic variation observed in the heading dates between ‘Nona Bokra’
and ‘Koshihikari’. Comparison of chromosomal locations between heading date QTLs detected in this study and QTLs previously
identified in ‘Nipponbare’ × ‘Kasalath’ populations revealed that eight of the heading date QTLs were recognized nearby the
Hd1, Hd2, Hd3a, Hd4, Hd5, Hd6, Hd9, and Hd13. These results suggest that the strong PS in ‘Nona Bokra’ was generated mainly by the accumulation of additive effects of
particular alleles at previously identified QTLs. 相似文献
5.
Kazuhiro Sato Kiyosumi Hori Kazuyoshi Takeda 《Molecular breeding : new strategies in plant improvement》2008,22(4):517-526
Fusarium head blight (FHB) resistance was evaluated in five recombinant inbred (RI) populations. The RI populations consisted
of top-cross progeny derived from a diallel set of crosses. Each of five two-row barley lines differing in response to FHB
were crossed with ‘Harbin 2-row’. FHB severity was scored on an 11-point scale, where resistant = 0 and susceptible = 10,
based on the ‘cut-spike test’. Disease data were obtained for each population for 2 or 3 years. Linkage maps comprised of
expressed sequence tag (EST) markers were developed for each population and used for quantitative trait locus (QTL) detection.
Thirty two QTLs were detected using all data sets (individual populations and years). Thirteen QTLs were detected using averages
across years; 10 of these were consistent across the individual year and average data sets. These QTLs clustered at 14 regions,
with clusters on all chromosomes. At 11 of these clusters, Harbin 2-row contributed FHB resistance alleles. No QTLs were detected
near the row type (vrs1) locus in any of the five RI populations, suggesting that the FHB resistance QTL in this region reported in two-row × six-row
crosses may be pleiotropic effect of vrs1. QTL were coincident with the flowering type locus (cly1/Cly2) on chromosome 2H in every population. Some QTL × QTL interactions were significant, but these were smaller than QTL main
effects. Considering the pleiotropic effect of spike morphology on FHB resistance, future FHB resistance mapping efforts in
barley should focus on cross combinations in which alleles at vrs1 are not segregating.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
6.
Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors 总被引:32,自引:0,他引:32
T. Yamamoto Y. Kuboki S. Y. Lin T. Sasaki M. Yano 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):37-44
Fine mapping was carried out on three putative QTLs (tentatively designated as Hd-1 to Hd-3) of five such QTLs controlling heading date in rice that had been earlier identified using an F2 population derived from a cross between a japonica variety, ‘Nipponbare’, and an indica variety, ‘Kasalath’, using progeny backcrossed with ‘Nipponbare’ as the recurrent parent. One BC3F2 and two BC3F1 plants, in which the target QTL regions were heterozygous and most other chromosomal regions were homozygous for the ‘Nipponbare’
allele, were selected as the experimental material. Self-pollinated progeny (BC3F2 and BC3F3) of the BC3F1 or BC3F2 showed continuous variation in days to heading. By means of progeny testing based on BC3F3 or BC3F4 lines, we determined the genotypes of each BC3F2 or BC3F3 individual at target QTLs. Their segregation patterns fitted Mendelian inheritance ratios. When the results obtained by RFLP
analysis and progeny tests were combined, Hd-1, Hd-2 and Hd-3 were mapped precisely on chromosomes 6, 7 and 6, respectively, of a rice RFLP linkage map. The results demonstrated that
QTLs can be treated as Mendelian factors. Moreover, these precise locations were in good agreement with the regions estimated
by QTL analysis of the initial F2 population, demonstrating the high reliability of QTL mapping using a high-density linkage map.
Received: 5 November 1997 / Accepted: 10 February 1998 相似文献
7.
L. Albar M. Lorieux N. Ahmadi I. Rimbault A. Pinel A. A. Sy D. Fargette A. Ghesquière 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(7):1145-1154
Rice yellow mottle virus (RYMV) resistance QTLs were mapped in a doubled-haploid population of rice, ‘IR64/Azucena’. Disease
impact on plant morphology and development, expression of symptoms and virus content were evaluated in field conditions. Virus
content was also assessed in a growth chamber. RYMV resistance was found to be under a polygenic determinism, and 15 QTLs
were detected on seven chromosomal fragments. For all of the resistance QTLs detected, the favourable allele was provided
by the resistant parent ‘Azucena’. Three regions were determined using different resistance parameters and in two environments.
On chromosome 12, a QTL of resistance that had already been detected in this population and another indica/japonica population was confirmed both in the field and under controlled conditions. Significant correlations were observed between
resistance and tillering ability, as measured on control non-inoculated plants. In addition, the three genomic fragments involved
in resistance were also involved in plant architecture and development. In particular, the semi-dwarfing gene sd-1, on chromosome 1, provided by the susceptible parent, ‘IR64’, mapped in a region where resistance QTLs were detected with
most of the resistance parameters. In contrast, the QTL of resistance mapped on chromosome 12 was found to be independent
of plant morphology.
Received: 20 April 1998 / Accepted: 30 April 1998 相似文献
8.
Riaz S Tenscher AC Ramming DW Walker MA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(6):1059-1073
A limited genetic mapping strategy based on simple sequence repeat (SSR) marker data was used with five grape populations
segregating for powdery mildew (Erysiphe necator) resistance in an effort to develop genetic markers from multiple sources and enable the pyramiding of resistance loci. Three
populations derived their resistance from Muscadinia rotundifolia ‘Magnolia’. The first population (06708) had 97 progeny and was screened with 137 SSR markers from seven chromosomes (4,
7, 9, 12, 13, 15, and 18) that have been reported to be associated with powdery or downy mildew resistance. A genetic map
was constructed using the pseudo-testcross strategy and QTL analysis was carried out. Only markers from chromosome 13 and
18 were mapped in the second (04327) and third (06712) populations, which had 47 and 80 progeny, respectively. Significant
QTLs for powdery mildew resistance with overlapping genomic regions were identified for different tissue types (leaf, stem,
rachis, and berry) on chromosome 18, which distinguishes the resistance in ‘Magnolia’ from that present in other accessions
of M. rotundifolia and controlled by the Run1 gene on chromosome 12. The ‘Magnolia’ resistance locus was termed as Run2.1. Powdery mildew resistance was also mapped in a fourth population (08391), which had 255 progeny and resistance from M. rotundifolia ‘Trayshed’. A locus accounting for 50% of the phenotypic variation mapped to chromosome 18 and was named Run2.2. This locus overlapped the region found in the ‘Magnolia’-based populations, but the allele sizes of the flanking markers
were different. ‘Trayshed’ and ‘Magnolia’ shared at least one allele for 68% of the tested markers, but alleles of the other
32% of the markers were not shared indicating that the two M. rotundifolia selections were very different. The last population, 08306 with 42 progeny, derived its resistance from a selection Vitis romanetii C166-043. Genetic mapping discovered a major powdery mildew resistance locus termed Ren4 on chromosome 18, which explained 70% of the phenotypic variation in the same region of chromosome 18 found in the two M. rotundifolia resistant accessions. The mapping results indicate that powdery mildew resistance genes from different backgrounds reside
on chromosome 18, and that genetic markers can be used as a powerful tool to pyramid these loci and other powdery mildew resistance
loci into a single line. 相似文献
9.
U. Kumar A. K. Joshi S. Kumar R. Chand M. S. R?der 《Molecular breeding : new strategies in plant improvement》2010,26(3):477-491
Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat in warm and humid wheat growing regions of the world. To identify quantitative trait loci
(QTLs) for spot blotch resistance, two mapping populations were developed by making the crosses between common susceptible
cultivar ‘Sonalika’ with the resistant breeding lines ‘Ning 8201’ and ‘Chirya 3’. Single seed descent derived F6, F7, F8 lines of the first cross ‘Ning 8201’ × ‘Sonalika’ were evaluated for resistance to spot blotch in three blocks in each of
the 3 years. After screening of 388 pairs of simple sequence repeat primers between the two parents, 119 polymorphic markers
were used to genotype the mapping population. Four QTLs were identified on the chromosomes 2AS, 2BS, 5BL and 7DS and explained
62.9% of phenotypic variation in a simultaneous fit. The QTL on chromosome 2A was detected only in 1 year and explained 22.7%
of phenotypic variation. In the second cross (‘Chirya 3’ × ‘Sonalika’), F7 and F8 population were evaluated in three blocks in each of the 2 years. In this population, five QTLs were identified on chromosomes
2BS, 2DS, 3BS, 7BS and 7DS. The QTLs identified in the ‘Chirya 3’ × ‘Sonalika’ population explained 43.4% of phenotypic variation
in a simultaneous fit. The alleles for reduced disease severity in both the populations were derived from the respective resistant
parent. The QTLs QSb.bhu-2B and QSb.bhu-7D from both populations were placed in the same deletion bins, 2BS1-0.53-0.75 and 7DS5-0.36-0.61, respectively. The closely
linked markers Xgwm148 to the QTL on chromosome 2B and Xgwm111 to the QTL on chromosome 7D are potentially diagnostic markers for spot blotch resistance. 相似文献
10.
H. El Attari P. M. Hayes A. Rebai G. Barrault G. Dechamp-Guillaume A. Sarrafi 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):95-100
Genetic variability for partial resistance to bacterial leaf streak in barley, caused by Xanthomonas campestris pv. hordei, was investigated in 119 doubled-haploid lines (DH) developed by the Hordeum bulbosum method from the F1 progeny of the cross between two cultivars, ‘Morex’ (resistant) and ‘Steptoe’ (susceptible). Two experiments were undertaken
in a randomized complete block design with three replicates, in a controlled growth chamber. Twenty seeds per replicate were
planted in plastic containers (60×40×8 cm) containing moistened vermiculite. At the two-leaf stage seedlings were inoculated
with an Iranian strain of the pathogen. Genetic variability was observed among the 119 DH lines for partial resistance to
the disease. Some DH lines were significantly more resistant than ‘Morex’ (resistant parent) to bacterial leaf streak. Genetic
gain in percentage of resistant parent for 5% of the selected DH lines was significant (47.70% and 33.72% in the first and
the second experiment, respectively). A QTL analysis of bacterial leaf streak resistance showed that three QTLs were detected
on chromosomes 3 and 7. Multilocus allelic effects of the three QTLs account for almost 54% of the mean difference between
the parents and nearly 30% of the phenotypic variation of the trait in the mean experiment. The resistance locus on chromosome
3, near ABG377, apprears to be a major gene.
Received: 15 July 1997 / Accepted: 4 August 1997 相似文献
11.
Uttam Kumar Arun K. Joshi Sundeep Kumar Ramesh Chand Marion S. Röder 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(4):783-792
Spot blotch caused by Bipolaris sorokiniana is a destructive disease of wheat in warm and humid wheat growing regions of the world. The development of disease resistant
cultivars is considered as the most effective control strategy for spot blotch. An intervarietal mapping population in the
form of recombinant inbred lines (RILs) was developed from a cross ‘Yangmai 6’ (a Chinese source of resistance) × ‘Sonalika’
(a spot blotch susceptible cultivar). The 139 single seed descent (SSD) derived F6, F7, F8 lines of ‘Yangmai 6’ × ‘Sonalika’ were evaluated for resistance to spot blotch in three blocks in each of the 3 years. Joint
and/or single year analysis by composite interval mapping (CIM) and likelihood of odd ratio (LOD) >2.2, identified four quantitative
trait loci (QTL) on the chromosomes 2AL, 2BS, 5BL and 6DL. These QTLs were designated as QSb.bhu-2A, QSb.bhu-2B, QSb.bhu-5B and QSb.bhu-6D, respectively. A total of 63.10% of phenotypic variation was explained by these QTLs based on the mean over years. Two QTLs
on chromosomes 2B and 5B with major effects were consistent over 3 years. All QTL alleles for resistance were derived from
the resistant parent ‘Yangmai 6’. 相似文献
12.
Fusarium head blight (FHB) is one of the most devastating wheat diseases, causing both yield loss and quality reduction. To detect
quantitative trait loci (QTL) responsible for FHB resistance, plants of the F
2:3 population derived from a ‘Wangshui-bai’ × ‘Sy95-7’ cross were artificially inoculated. Of 396 simple sequence repeats (SSRs),
125 amplified fragment length polymorphisms were used for FHB resistance QTL analysis. Five QTLs for FHB resistance were detected
on chromosomes 3B, 6B, 7A, 1B and 2D. The effect of the QTL located on chromosome 3B on phenotypic variation was 31.69%, while
that of the QTL found on 2D was the smallest and only accounted for 4.98% of the variation. The resistance alleles originated
from ‘Wangshibai’ and association of the QTLs using these SSR markers may facilitate marker-assisted selection to improve
FHB resistance in the wheat breeding programs of southwest China. 相似文献
13.
Di Gaspero G Cipriani G Adam-Blondon AF Testolin R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(7):1249-1263
Genetic maps functionally oriented towards disease resistance have been constructed in grapevine by analysing with a simultaneous
maximum-likelihood estimation of linkage 502 markers including microsatellites and resistance gene analogs (RGAs). Mapping
material consisted of two pseudo-testcrosses, ‘Chardonnay’ × ‘Bianca’ and ‘Cabernet Sauvignon’ × ‘20/3’ where the seed parents
were Vitis vinifera genotypes and the male parents were Vitis hybrids carrying resistance to mildew diseases. Individual maps included 320–364 markers each. The simultaneous use of two
mapping crosses made with two pairs of distantly related parents allowed mapping as much as 91% of the markers tested. The
integrated map included 420 Simple Sequence Repeat (SSR) markers that identified 536 SSR loci and 82 RGA markers that identified
173 RGA loci. This map consisted of 19 linkage groups (LGs) corresponding to the grape haploid chromosome number, had a total
length of 1,676 cM and a mean distance between adjacent loci of 3.6 cM. Single-locus SSR markers were randomly distributed
over the map (CD = 1.12). RGA markers were found in 18 of the 19 LGs but most of them (83%) were clustered on seven LGs, namely
groups 3, 7, 9, 12, 13, 18 and 19. Several RGA clusters mapped to chromosomal regions where phenotypic traits of resistance
to fungal diseases such as downy mildew and powdery mildew, bacterial diseases such as Pierce’s disease, and pests such as
dagger and root-knot nematode, were previously mapped in different segregating populations. The high number of RGA markers
integrated into this new map will help find markers linked to genetic determinants of different pest and disease resistances
in grape.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
14.
Both stable and unstable QTLs for resistance to powdery mildew are detected in apple after four years of field assessments 总被引:1,自引:0,他引:1
Powdery mildew, caused by the ascomycete fungus Podosphaera leucotricha, is one of the most damaging diseases of apple worldwide. Polygenically determined resistance might contribute to a significant increase of resistance to this disease in new cultivars. A quantitative trait locus (QTL) analysis was performed in an F1 progeny derived from a cross between the apple cultivar Discovery and the apple hybrid TN10-8. Powdery mildew incidence was assessed during four years (five seasons) in spring and/or autumn in a French local orchard. Seven additive and/or dominant QTLs were detected over the five seasons, with effects (R
2) ranging from 7.5% to 27.4% of the progeny phenotypic variation. Two QTLs, on linkage groups (LGs) 2 and 13, were consistently identified and accounted together from 29% to 37% of the phenotypic variation according to the year of assessment. The other QTLs were identified during one (LGs 1, 14), two (LG10), or three (LGs 8, 17) seasons. Their instability indicated a changing genetic determinism according to the year of assessment, for which several hypotheses may be put forward. The QTLs on LGs 2 and 8 mapped close to clusters of resistance gene analogs (RGAs) and major genes for resistance to mildew or apple scab previously identified. The stable QTLs identified on LGs 2 and 13, together with the strong effect QTL located on LG 8, are of special interest for breeding purposes, especially if combined with other major resistance genes. 相似文献
15.
16.
Hoffmann S Di Gaspero G Kovács L Howard S Kiss E Galbács Z Testolin R Kozma P 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(3):427-438
Vitis vinifera ‘Kishmish vatkana’, a cultivated grapevine from Central Asia, does not produce visible symptoms in response to natural or
artificial inoculation with the fungus Erysiphe necator Schwein., the casual agent of powdery mildew. ‘Kishmish vatkana’ allowed pathogen entry into epidermal cells at a rate comparable
to that in the susceptible control Vitis vinifera ‘Nimrang’, but was able to limit subsequent hyphal proliferation. Density of conidiophores was significantly lower in ‘Kishmish
vatkana’ (33.6 ± 8.7 conidiophores mm−2) than in ‘Nimrang’ (310.5 ± 24.0 conidiophores mm−2) by 120 h after inoculation. A progeny of 310 plants from a ‘Nimrang’ × ‘Kishmish vatkana’ cross were scored for the presence
or absence of visible conidiophores throughout two successive seasons. Phenotypic segregation revealed the presence of a single
dominant allele termed Resistance to Erysiphe necator 1 (REN1), which was heterozygous in ‘Kishmish vatkana’. A bulked segregant analysis was carried out using 195 microsatellite markers
uniformly distributed across the entire genome. For each marker, association with the resistance trait was inferred by measuring
in the bulks the ratio of peak intensities of the two alleles inherited from ‘Kishmish vatkana’. The phenotypic locus was
assigned to linkage group 13, a genomic region in which no disease resistance had been reported previously. The REN1 position was restricted to a 7.4 cM interval by analyzing the 310 offspring for the segregation of markers that surrounded
the target region. The closest markers, VMC9H4-2, VMCNG4E10-1 and UDV-020, were located 0.9 cM away from the REN1 locus.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
17.
Muhammad A. Khan Brion Duffy Cesare Gessler Andrea Patocchi 《Molecular breeding : new strategies in plant improvement》2006,17(4):299-306
Fire blight caused by the bacterium Erwinia amylovora is a severe threat to apple and pear orchards worldwide. Apple varieties exhibit a wide range of relative susceptibility/tolerance to fire blight. Although, no monogenic resistance against fire blight has been identified yet, recent evidence indicates the existence of quantitative resistance. Potential sources of fire blight resistance include several wild Malus species and some apple cultivars. F1 progenies of ‘Fiesta’בDiscovery’ were inoculated with the Swiss strain Ea 610 and studied under controlled conditions to identify quantitative trait loci (QTLs) for fire blight resistance. Disease was evaluated at four time points after inoculation. Shoot lesion length and the area under disease progress curve (AUDPC) values were used for QTL analysis. One significant (LOD score of 7.5–8.1, p<0.001) QTL was identified on the linkage group 7 of ‘Fiesta’ (F7). The F7 QTL explained about 37.5–38.6% of the phenotypic variation. 相似文献
18.
Identification and molecular mapping of PdR1, a primary resistance gene to Pierce’s disease in Vitis
Krivanek AF Riaz S Walker MA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(6):1125-1131
A major quantitative trait locus (QTL) controlling resistance to Pierce’s disease (PD) of grape, caused by the bacterium Xylella fastidiosa (Xf), was identified on a Vitis linkage map and denoted as ‘Pierce’s disease resistance 1’ (PdR1). Placement of the locus was accomplished by evaluating a family of full-sib progeny from a cross of two PD-resistant interspecific
hybrids with resistance inherited from Vitis arizonica. Resistance was measured under greenhouse conditions by direct quantification of Xf numbers in stem tissues as well as by
evaluation of disease symptoms based on leaf scorch and a cane maturation index (CMI). A large QTL (LOD 17.2) accounting for
72% of the phenotypic variance in bacterial numbers was localized to linkage group 14 of the male parent F8909-17. The approximate
95% confidence interval around the QTL peak extended 5.7 cM when using composite interval mapping. The other disease evaluation
methods (leaf scorch and CMI, respectively) placed the resistance QTL to the same region on linkage group 14, although at
wider 95% confidence intervals (6.0 and 7.5 cM), lower peak LOD scores (11.9 and 7.7) and accounting for less phenotypic variance
(59 and 42%). This is the first report of an Xf resistance QTL mapped in any crop species. The relevance of the markers located
in the region spanning the QTL will be discussed, addressing their usefulness for the development of PD-resistant grape cultivars. 相似文献
19.
Sibylle Stoeckli Karsten Mody Cesare Gessler Andrea Patocchi Mauro Jermini Silvia Dorn 《Tree Genetics & Genomes》2008,4(4):833-847
The rosy apple aphid (Dysaphis plantaginea), the leaf-curling aphid (Dysaphis cf. devecta) and the green apple aphid (Aphis pomi) are widespread pest insects that reduce growth of leaves, fruits and shoots in apple (Malus × domestica). Aphid control in apple orchards is generally achieved by insecticides, but alternative management options like growing
resistant cultivars are needed for a more sustainable integrated pest management (IPM). A linkage map available for a segregating
F1-cross of the apple cultivars ‘Fiesta’ and ‘Discovery’ was used to investigate the genetic basis of resistance to aphids.
Aphid infestation and plant growth characteristics were repeatedly assessed for the same 160 apple genotypes in three different
environments and 2 consecutive years. We identified amplified fragment length polymorphism (AFLP) markers linked to quantitative
trait loci (QTLs) for resistance to D. plantaginea (‘Fiesta’ linkage group 17, locus 57.7, marker E33M35–0269; heritability: 28.3%), and to D. cf. devecta (‘Fiesta’ linkage group 7, locus 4.5, marker E32M39–0195; heritability: 50.2%). Interactions between aphid species, differences
in climatic conditions and the spatial distribution of aphid infestation were identified as possible factors impeding the
detection of QTLs. A pedigree analysis of simple sequence repeat (SSR) marker alleles closely associated with the QTL markers
revealed the presence of the alleles in other apple cultivars with reported aphid resistance (‘Wagener’, ‘Cox’s Orange Pippin’),
highlighting the genetic basis and also the potential for gene pyramiding of aphid resistance in apple. Finally, significant
QTLs for shoot length and stem diameter were identified, while there was no relationship between aphid resistance and plant
trait QTLs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
20.
Simple sequence repeat markers linked to QTL for resistance to Watermelon mosaic virus in melon 总被引:1,自引:0,他引:1
Palomares-Rius FJ Viruel MA Yuste-Lisbona FJ López-Sesé AI Gómez-Guillamón ML 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(7):1207-1214
A population of recombinant inbred lines (RIL) derived from a cross between the Watermelon mosaic virus (WMV) resistant genotype TGR-1551 and the susceptible Spanish cultivar ‘Bola de Oro’ has been evaluated for WMV resistance
in spring, fall and growth chamber conditions. The quantitative trait loci (QTL) analyses detected one major QTL (wmv) on linkage group (LG) XI close to the microsatellite marker CMN04_35. This QTL controls the resistance to WMV in the three
environmental conditions evaluated. Other minor QTLs affecting the severity of viral symptoms were identified, but they were
not detected in all the assayed environments. The screening of the marker CMN04_35 in an F2 progeny, derived from the same cross, confirmed the effect of this QTL on the expression of WMV resistance also in early
generations, which evidences the usefulness of this marker for a marker assisted selection program. 相似文献