共查询到20条相似文献,搜索用时 765 毫秒
1.
Laurent X Nouvel Tiago Dos Vultos Eric Kassa-Kelembho Jean Rauzier Brigitte Gicquel 《BMC microbiology》2007,7(1):39
Background
Previous studies have suggested that variations in DNA repair genes of W-Beijing strains may have led to transient mutator phenotypes which in turn may have contributed to host adaptation of this strain family. Single nucleotide polymorphism (SNP) in the DNA repair gene mutT1 was identified in MDR-prone strains from the Central African Republic. A Mycobacteriumtuberculosis H37Rv mutant inactivated in two DNA repair genes, namely ada/alkA and ogt, was shown to display a hypermutator phenotype. We then looked for polymorphisms in these genes in Central African Republic strains (CAR). 相似文献2.
3.
Background
The ubiquitous family of DnaN sliding processivity clamp proteins plays essential roles in DNA replication, DNA repair, and cell cycle progression, in part by managing the actions of the different proteins involved in these processes. Interactions of the homodimeric Escherichia coli β clamp with its known partners involves multiple surfaces, including a hydrophobic cleft located near the C-terminus of each clamp protomer. 相似文献4.
Background
Chromosome pairing, recombination and DNA repair are essential processes during meiosis in sexually reproducing organisms. Investigating the bread wheat (Triticum aestivum L.) Ph2 (Pairing homoeologous) locus has identified numerous candidate genes that may have a role in controlling such processes, including TaMSH7, a plant specific member of the DNA mismatch repair family. 相似文献5.
Background
Caenorhabditis elegans is an important model for the study of DNA damage and repair related processes such as aging, neurodegeneration, and carcinogenesis. However, DNA repair is poorly characterized in this organism. We adapted a quantitative polymerase chain reaction assay to characterize repair of DNA damage induced by ultraviolet type C (UVC) radiation in C. elegans, and then tested whether DNA repair rates were affected by age in adults. 相似文献6.
Background
DNA repair is the general term for the collection of critical mechanisms which repair many forms of DNA damage such as methylation or ionizing radiation. DNA repair has mainly been studied in experimental and clinical situations, and relatively few information-based approaches to new extracting DNA repair knowledge exist. As a first step, automatic detection of DNA repair proteins in genomes via informatics techniques is desirable; however, there are many forms of DNA repair and it is not a straightforward process to identify and classify repair proteins with a single optimal method. We perform a study of the ability of homology and machine learning-based methods to identify and classify DNA repair proteins, as well as scan vertebrate genomes for the presence of novel repair proteins. Combinations of primary sequence polypeptide frequency, secondary structure, and homology information are used as feature information for input to a Support Vector Machine (SVM). 相似文献7.
Bastian Omokoko Uwe K Jäntges Martin Zimmermann Monika Reiss Winfried Hartmeier 《BMC microbiology》2008,8(1):197
Background
Geobacillus stearothermophilus is able to utilize phenol as a sole carbon source. A DNA fragment encoding a phenol hydroxylase catalyzing the first step in the meta-pathway has been isolated previously. Based on these findings a PCR-based DNA walk was performed initially to isolate a catechol 2,3-dioxygenase for biosensoric applications but was continued to elucidate the organisation of the genes encoding the proteins for the metabolization of phenol. 相似文献8.
Katrina L Tibballs Ole Herman Ambur Kristian Alfsnes H?vard Homberset Stephan A Frye Tonje Davidsen Tone T?njum 《BMC microbiology》2009,9(1):7
Background
Neisseria meningitidis, the causative agent of meningococcal disease, is exposed to high levels of reactive oxygen species inside its exclusive human host. The DNA glycosylase Fpg of the base excision repair pathway (BER) is a central player in the correction of oxidative DNA damage. This study aimed at characterizing the meningococcal Fpg and its role in DNA repair. 相似文献9.
Kira S Makarova Nick V Grishin Svetlana A Shabalina Yuri I Wolf Eugene V Koonin 《Biology direct》2006,1(1):7-26
Background
All archaeal and many bacterial genomes contain Clustered Regularly Interspaced Short Palindrome Repeats (CRISPR) and variable arrays of the CRISPR-associated (cas) genes that have been previously implicated in a novel form of DNA repair on the basis of comparative analysis of their protein product sequences. However, the proximity of CRISPR and cas genes strongly suggests that they have related functions which is hard to reconcile with the repair hypothesis. 相似文献10.
Hsu-Feng Lu Tung-Yuan Lai Te-Chung Hsia Yih-Jing Tang Jai-Sing Yang Jo-Hua Chiang Chi-Cheng Lu Chi-Ming Liu Hai-Lung Wang Jing-Gung Chung 《Neurochemical research》2010,35(7):1105-1110
Our primary studies had shown that danthron induced cytotoxic effects, including apoptosis and inhibition of migration and
invasion. However, danthron-affected DNA damage and repair gene expressions are not clear. In this study, we investigated
to examine whether or not danthron induced DNA damage and inhibited DNA repair gene expression in human brain glioblastoma
multiforms (GBM 8401) cells. The results from Comet assay indicated that incubation of GBM 8401 cells with 0, 50, 100 and
150 μM of danthron led to a longer DNA migration smear based on the single cell electrophoresis (Comet tail). The results
from real-time PCR assay demonstrated that 100 μM of danthron for 24 h treatment in GBM 8401 cells led to decrease all examined
ataxia telangiectasia mutated (ATM), ataxia-telangiectasia and Rad3-related (ATR), breast cancer 1, early onset (BRCA-1),
14-3-3 proteins sigma (14-3-3σ), DNA-dependent serine/threonine protein kinase (DNA-PK) and O
6
-methylguanine-DNA methyltransferase (MGMT) mRNA expressions. Taken together, the present study showed that danthron caused
DNA damage and inhibited DNA repair genes, which may be the factors for danthron-inhibited cell growth in vitro. 相似文献
11.
Renata Krupa Anna Sobczuk Tomasz Popławski Katarzyna Wozniak Janusz Blasiak 《Molecular biology reports》2011,38(2):1163-1170
The cellular reaction to the DNA-damaging agents may modulate individual’s cancer susceptibility. This reaction is mainly
determined by the efficacy of DNA repair, which in turn, may be influenced by the variability of DNA repair genes, expressed
by their polymorphism. The hOGG1 gene encodes a glycosylase of base excision repair and RAD51 specifies a key protein in homologues recombination repair. Both proteins can be involved in the repair of DNA lesions, which
are known to contribute to endometrial cancer. In the present work we determined the extent of basal DNA damage and the efficacy
of removal of DNA damage induced by hydrogen peroxide and N-methyl-N′-nitro N-nitrosoguanidyne (MNNG) in peripheral blood
lymphocytes of 30 endometrial cancer patients and 30 individuals without cancer. The results from DNA damage and repair study
were correlated with the genotypes of two common polymorphisms of the hOGG1 and RAD51 genes: a G>C transversion at 1245 position of the hOGG1 gene producing a Ser → Cys substitution at the codon 326 (the Ser326Cys polymorphism) and a G>C substitution at 135 position
of the RAD51 gene (the 135G>C polymorphism). DNA damage and repair were evaluated by alkaline single cell gel electrophoresis and genotypes
were determined by restriction fragment length polymorphism PCR. We observed a strong association between endometrial cancer
and the C/C genotype of the 135G>C polymorphism of the RAD51 gene. Moreover, there was a strong correlation between that genotype and endometrial cancer occurrence in subjects with a
high level of basal DNA damage. We did not observe any correlation between the Ser326Cys polymorphism of the hOGG1 gene and endometrial cancer. Our result suggest that the 135G>C polymorphism of the RAD51 gene may be linked to endometrial cancer and can be considered as an additional marker of this disease. 相似文献
12.
Janet Yee Anita Tang Wei-Ling Lau Heather Ritter Dewald Delport Melissa Page Rodney D Adam Miklós Müller Gang Wu 《BMC molecular biology》2007,8(1):26
Background
Giardia intestinalis is a protist found in freshwaters worldwide, and is the most common cause of parasitic diarrhea in humans. The phylogenetic position of this parasite is still much debated. Histones are small, highly conserved proteins that associate tightly with DNA to form chromatin within the nucleus. There are two classes of core histone genes in higher eukaryotes: DNA replication-independent histones and DNA replication-dependent ones. 相似文献13.
14.
Background
Enzymes involved in DNA metabolic events of the highly radioresistant bacterium Deinococcus radiodurans are currently examined to understand the mechanisms that protect and repair the Deinococcus radiodurans genome after extremely high doses of γ-irradiation. Although several Deinococcus radiodurans DNA repair enzymes have been characterised, no biochemical data is available for DNA ligation and DNA endhealing enzymes of Deinococcus radiodurans so far. DNA ligases are necessary to seal broken DNA backbones during replication, repair and recombination. In addition, ionizing radiation frequently leaves DNA strand-breaks that are not feasible for ligation and thus require end-healing by a 5'-polynucleotide kinase or a 3'-phosphatase. We expect that DNA ligases and end-processing enzymes play an important role in Deinococcus radiodurans DNA strand-break repair. 相似文献15.
Background
How DNA repair enzymes find the relatively rare sites of damage is not known in great detail. Recent experiments and molecular data suggest that individual repair enzymes do not work independently of each other, but interact with each other through charges exchanged along the DNA. A damaged site in the DNA hinders this exchange. The hypothesis is that the charge exchange quickly liberates the repair enzymes from error-free stretches of DNA. In this way, the sites of damage are located more quickly; but how much more quickly is not known, nor is it known whether the charge exchange mechanism has other observable consequences. 相似文献16.
17.
Background
Oxygenic photosynthesis is accompanied by the formation of reactive oxygen species (ROS), which damage proteins, lipids, DNA and finally limit plant yield. The enzymes of the chloroplast antioxidant system are exclusively nuclear encoded. During evolution, plastid and mitochondrial genes were post-endosymbiotically transferred to the nucleus, adapted for eukaryotic gene expression and post-translational protein targeting and supplemented with genes of eukaryotic origin. 相似文献18.
Background
Efficient and correct repair of DNA damage, especially DNA double-strand breaks, is critical for cellular survival. Defects in the DNA repair may lead to cell death or genomic instability and development of cancer. Non-homologous end-joining (NHEJ) is the major repair pathway for DNA double-strand breaks in mammalian cells. The ability of other repair pathways, such as homologous recombination, to compensate for loss of NHEJ and the ways in which contributions of different pathways are regulated are far from fully understood. 相似文献19.
Sushant K. Kachhap Nadine Rosmus Spencer J. Collis Madeleine S. Q. Kortenhorst Michel D. Wissing Mohammad Hedayati Shabana Shabbeer Janet Mendonca Justin Deangelis Luigi Marchionni Jianqing Lin Naseruddin H?ti Johan W. R. Nortier Theodore L. DeWeese Hans Hammers Michael A. Carducci 《PloS one》2010,5(6)
20.
Theresa Gorsler Ulrike Murzik Tobias Ulbricht Julia Hentschel Peter Hemmerich Christian Melle 《BMC cell biology》2010,11(1):100