共查询到8条相似文献,搜索用时 31 毫秒
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Christopher Heuer JohnAlexander Preuß Taieb Habib Anton Enders Janina Bahnemann 《Engineering in Life Science》2022,22(12):744
Since its invention in the 1980s, 3D printing has evolved into a versatile technique for the additive manufacturing of diverse objects and tools, using various materials. The relative flexibility, straightforwardness, and ability to enable rapid prototyping are tremendous advantages offered by this technique compared to conventional methods for miniaturized and microfluidic systems fabrication (such as soft lithography). The development of 3D printers exhibiting high printer resolution has enabled the fabrication of accurate miniaturized and microfluidic systems—which have, in turn, substantially reduced both device sizes and required sample volumes. Moreover, the continuing development of translucent, heat resistant, and biocompatible materials will make 3D printing more and more useful for applications in biotechnology in the coming years. Today, a wide variety of 3D‐printed objects in biotechnology—ranging from miniaturized cultivation chambers to microfluidic lab‐on‐a‐chip devices for diagnostics—are already being deployed in labs across the world. This review explains the 3D printing technologies that are currently used to fabricate such miniaturized microfluidic devices, and also seeks to offer some insight into recent developments demonstrating the use of these tools for biotechnological applications such as cell culture, separation techniques, and biosensors. 相似文献
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Although the microbiology laboratory paradigm has increasingly changed from manual to automated procedures, and from functional to molecular methods, traditional culture methods remain vital. Using inexpensive desktop fused filament fabrication 3D printing, we designed, produced and tested rapid prototypes of customised labware for microbial culture namely frames to make dip slides, inoculation loops, multi-pin replicators, and multi-well culture plates for solid medium. These customised components were used to plate out samples onto solid media in various formats, and we illustrate how they can be suitable for many microbiological methods such as minimum inhibitory concentration tests, or for directly detecting pathogens from mastitis samples, illustrating the flexibility of rapid-prototyped culture consumable parts for streamlining microbiological methods. We describe the methodology needed for microbiologists to develop their own novel and unique tools, or to fabricate and customise existing consumables. A workflow is presented for designing and 3D printing labware and quickly producing easy-to-sterilise and re-useable plastic parts of great utility in the microbiology laboratory. 相似文献
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M. M. P. Driessen E. Kort M. J. M. Cramer P. A. Doevendans M. J. Angevaare T. Leiner F. J. Meijboom S. A. J. Chamuleau G. Tj Sieswerda 《Netherlands heart journal》2014,22(9):383-390
Aims
This study is the first to directly compare two widely used real-time 3D echocardiography (RT3DE) methods of cardiac magnetic resonance imaging (CMR) and assess their reproducibility in experienced and less experienced observers.Methods
Consecutive patients planned for CMR underwent RT3DE within 8 h of CMR with Philips (volumetric method) and Toshiba Artida (speckle tracking method). Left ventricular ejection fraction (LVEF), left ventricular end-diastolic volume (LVEDV) and end-systolic volume (LVESV) were measured using RT3DE, by four trained observers, and compared with CMR values.Results
Thirty-five patients were included (49.7 ± 15.7 years; 55 % male), 30 (85.7 %) volumetric and 27 (77.1 %) speckle tracking datasets could be analysed. CMR derived LVEDV, LVESV and LVEF were 198 ± 58 ml, 106 ± 53 ml and 49 ± 15 %, respectively. LVEF derived from speckle tracking was accurate and reproducible in all observers (all intra-class correlation coefficients (ICC) > 0.86). LVEF derived from the volumetric method correlated well to CMR in experienced observers (ICC 0.85 and 0.86) but only moderately in less experienced observers (ICC 0.58 and 0.77) and was less reproducible in these observers (ICC = 0.55). Volumes were significantly underestimated compared with CMR (p < 0.001).Conclusion
This study demonstrates that both RT3DE methodologies are sufficiently accurate and reproducible for use in daily practice. However, experience importantly influences the accuracy and reproducibility of the volumetric method, which should be considered when introducing this technique into clinical practice. 相似文献7.
摘要 目的:探讨术前使用左心耳3D模型及左心耳封堵体外模拟治疗心房颤动患者的疗效。方法:回顾性选择2021年1月至2022年10月来我院诊治的心房颤动患者,对照组行经食管超声心动图检查、心脏CT造影检查及左心耳封堵术。观察组行经食管超声心动图检查、心脏CT造影检查、基于心脏CT造影数据打印左心耳3D模型、应用3D打印模型进行左心耳封堵体外模拟演练及左心耳封堵术,对比两组相关指标。结果:两组经食管超声心动图检查左心耳各径线对比无差异(P>0.05)。两组经CT检查左心耳各径线对比无差异(P>0.05)。两组封堵器固定大小对比有差异(P<0.05)。术后经食管超声心动图检查发现,观察组术后3个月及12个月血流通过封堵器几率高于对照组,但组间对比无差异(P>0.05)。术后3个月及12个月时,两组的左心房内径降低,且同时间点观察组较对照组(P<0.05);两组术后3个月及12个月时左心室内径对比无差异(P>0.05)。观察组的封堵器型号匹配率较对照组高,手术操作时间、X线曝光量、造影剂使用量、封堵器术中释放次数较对照组低(P<0.05),观察组的残余分流及手术并发症较对照组低,但组间对比无差异(P>0.05)。观察组术后3个月封堵器位置、有无残腔及不良心血管事件与对照组相比无差异(P>0.05),术后12个月,封堵器位置良好率较对照组低(P<0.05),其余两组间对比无差异(P>0.05)。结论:术前使用左心耳3D模型及左心耳封堵体外模拟可提高心房颤动患者的疗效。 相似文献
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Sulfhydryl‐Based Inhibition of δ‐ALA‐D and Na+, K+‐ATPase Activities Depends on the Organoselenium Group Bonded to the Isoquinoline 下载免费PDF全文
Tuane Bazanella Sampaio Juliana Trevisan da Rocha Caroline Brandão Quines André Luiz Agnes Stein Gilson Zeni Cristina Wayne Nogueira 《Journal of cellular biochemistry》2017,118(5):1144-1150