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Arabidopsis HIPP27 is a host susceptibility gene for the beet cyst nematode Heterodera schachtii 总被引:1,自引:0,他引:1 下载免费PDF全文
Zoran S. Radakovic Muhammad Shahzad Anjam Elizabeth Escobar Divykriti Chopra Javier Cabrera Ana Cláudia Silva Carolina Escobar Miroslaw Sobczak Florian M. W. Grundler Shahid Siddique 《Molecular Plant Pathology》2018,19(8):1917-1928
Sedentary plant‐parasitic cyst nematodes are obligate biotrophs that infect the roots of their host plant. Their parasitism is based on the modification of root cells to form a hypermetabolic syncytium from which the nematodes draw their nutrients. The aim of this study was to identify nematode susceptibility genes in Arabidopsis thaliana and to characterize their roles in supporting the parasitism of Heterodera schachtii. By selecting genes that were most strongly upregulated in response to cyst nematode infection, we identified HIPP27 (HEAVY METAL‐ASSOCIATED ISOPRENYLATED PLANT PROTEIN 27) as a host susceptibility factor required for beet cyst nematode infection and development. Detailed expression analysis revealed that HIPP27 is a cytoplasmic protein and that HIPP27 is strongly expressed in leaves, young roots and nematode‐induced syncytia. Loss‐of‐function Arabidopsis hipp27 mutants exhibited severely reduced susceptibility to H. schachtii and abnormal starch accumulation in syncytial and peridermal plastids. Our results suggest that HIPP27 is a susceptibility gene in Arabidopsis whose loss of function reduces plant susceptibility to cyst nematode infection without increasing the susceptibility to other pathogens or negatively affecting the plant phenotype. 相似文献
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Mi‐Ok Woo Hunter Beard Margaret H. MacDonald Eric P. Brewer Reham M. Youssef Hyunsoon Kim Benjamin F. Matthews 《Molecular Plant Pathology》2014,15(9):927-939
Plant endo‐β‐1,4‐glucanases (EGases) include cell wall‐modifying enzymes that are involved in nematode‐induced growth of syncytia (feeding structures) in nematode‐infected roots. EGases in the α‐ and β‐subfamilies contain signal peptides and are secreted, whereas those in the γ‐subfamily have a membrane‐anchoring domain and are not secreted. The Arabidopsis α‐EGase At1g48930, designated as AtCel6, is known to be down‐regulated by beet cyst nematode (Heterodera schachtii) in Arabidopsis roots, whereas another α‐EGase, AtCel2, is up‐regulated. Here, we report that the ectopic expression of AtCel6 in soybean roots reduces susceptibility to both soybean cyst nematode (SCN; Heterodera glycines) and root knot nematode (Meloidogyne incognita). Suppression of GmCel7, the soybean homologue of AtCel2, in soybean roots also reduces the susceptibility to SCN. In contrast, in studies on two γ‐EGases, both ectopic expression of AtKOR2 in soybean roots and suppression of the soybean homologue of AtKOR3 had no significant effect on SCN parasitism. Our results suggest that secreted α‐EGases are likely to be more useful than membrane‐bound γ‐EGases in the development of an SCN‐resistant soybean through gene manipulation. Furthermore, this study provides evidence that Arabidopsis shares molecular events of cyst nematode parasitism with soybean, and confirms the suitability of the Arabidopsis–H. schachtii interaction as a model for the soybean–H. glycines pathosystem. 相似文献
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A cyst nematode effector binds to diverse plant proteins,increases nematode susceptibility and affects root morphology 总被引:1,自引:0,他引:1 下载免费PDF全文
Gennady Pogorelko Parijat S. Juvale William B. Rutter Tarek Hewezi Richard Hussey Eric L. Davis Melissa G. Mitchum Thomas J. Baum 《Molecular Plant Pathology》2016,17(6):832-844
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Megan E. Andriankaja Selahattin Danisman Lorin F. Mignolet-Spruyt Hannes Claeys Irina Kochanke Mattias Vermeersch Liesbeth De Milde Stefanie De Bodt Veronique Storme Aleksandra Skirycz Felix Maurer Petra Bauer Per Mühlenbock Frank Van Breusegem Gerco C. Angenent Richard G. H. Immink Dirk Inzé 《Plant molecular biology》2014,85(3):233-245
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The Arabidopsis immune regulator SRFR1 dampens defences against herbivory by Spodoptera exigua and parasitism by Heterodera schachtii 下载免费PDF全文
Phuong Dung T. Nguyen Sharon Pike Jianying Wang Arati Nepal Poudel Robert Heinz Jack C. Schultz Abraham J. Koo Melissa G. Mitchum Heidi M. Appel Walter Gassmann 《Molecular Plant Pathology》2016,17(4):588-600
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Suppression of NGB and NAB/ERabp1 in tomato modifies root responses to potato cyst nematode infestation 下载免费PDF全文
Joanna Dąbrowska‐Bronk Magdalena Czarny Anita Wiśniewska Sylwia Fudali Łukasz Baranowski Mirosław Sobczak Magdalena Święcicka Mateusz Matuszkiewicz Grzegorz Brzyżek Tadeusz Wroblewski Renata Dobosz Grzegorz Bartoszewski Marcin Filipecki 《Molecular Plant Pathology》2015,16(4):334-348
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The DYT1‐interacting proteins bHLH010, bHLH089 and bHLH091 are redundantly required for Arabidopsis anther development and transcriptome 下载免费PDF全文
Engao Zhu Chenjiang You Shuangshuang Wang Jie Cui Baixiao Niu Yingxiang Wang Ji Qi Hong Ma Fang Chang 《The Plant journal : for cell and molecular biology》2015,83(6):976-990
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Muhammad Amjad Ali Stephan Plattner Zoran Radakovic Krzysztof Wieczorek Abdelnaser Elashry Florian M.W. Grundler Moritz Ammelburg Shahid Siddique Holger Bohlmann 《The Plant journal : for cell and molecular biology》2013,74(5):852-866
The beet cyst nematode Heterodera schachtii induces syncytia in the roots of Arabidopsis thaliana, which are its only nutrient source. One gene, At1g64110, that is strongly up‐regulated in syncytia as shown by RT‐PCR, quantitative RT‐PCR, in situ RT‐PCR and promoter::GUS lines, encodes an AAA+‐type ATPase. Expression of two related genes in syncytia, At4g28000 and At5g52882, was not detected or not different from control root segments. Using amiRNA lines and T‐DNA mutants, we show that At1g64110 is important for syncytium and nematode development. At1g64110 was also inducible by wounding, jasmonic acid, salicylic acid, heat and cold, as well as drought, sodium chloride, abscisic acid and mannitol, indicating involvement of this gene in abiotic stress responses. We confirmed this using two T‐DNA mutants that were more sensitive to abscisic acid and sodium chloride during seed germination and root growth. These mutants also developed significantly smaller roots in response to abscisic acid and sodium chloride. An in silico analysis showed that ATPase At1g64110 (and also At4g28000 and At5g52882) belong to the ‘meiotic clade’ of AAA proteins that includes proteins such as Vps4, katanin, spastin and MSP1. 相似文献
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The nematophagous fungus Dactylella oviparasitica is considered the primary cause of a sugar beet cyst nematode (Heterodera schachtii) population suppression in a field at the Agricultural Operations, University of California, Riverside. Parasitism of H. schachtii by the ascomycete D. oviparasitica was studied using both Arabidopsis thaliana (type Landsberg erecta) and cabbage as host plants in gnotobiotic agar culture. Suitability of Arabidopsis as a host for H. schachtii was confirmed using seedlings grown with the nematode in axenic sand culture. Both developing males and females of H. schachtii broke through the Arabidopsis root surface during late juvenile stages and both were susceptible to D. oviparasitica parasitism. In contrast to Arabidopsis, developing juvenile males remained in nearly all observed cases enclosed within the cabbage root tissues while the larger body expansion of the female juveniles caused the root cortex to split; consequently only the latter ones were accessible to the fungus. In the presence of D. oviparasitica, the number of females with eggs was reduced by more than 95% and the number of eggs per female by almost 60% as compared to females developing on plates without the fungus. Viable eggs were not susceptible to parasitism while more than 90% of heat- or cold-killed eggs were rendered susceptible. These observations suggest that parasitism of developing juveniles may be the essential mode of action in the population suppression of H. schachtii. 相似文献