Transmission of Salmonella montevideo in Wheat by Stored-Product Insects

Sitophilus oryzae (L.), S. granarius (L.), Tribolium castaneum (Hbst.), Oryzaephilus surinamensis (L.), Rhyzopertha dominica (F.), Tenebroides mauritanicus (L.), and Cryptolestes pusillus (Schon.) transmitted Salmonella montevideo from wheat contaminated with 10(6) organisms/g to clean wheat. The insects were fed on the contaminated grain for 21 days and were then transferred to clean grain and allowed to feed for 21 days. They were subsequently transferred to two more samples of clean wheat. All species carried S. montevideo into the initial sample of clean wheat but not into a second or third sample. Progeny of the original insects that developed in the contaminated wheat exhibited less ability than the original adults to contaminate clean wheat. Data indicated that few S. montevideo could be carried by the stored-product insects in large masses of grain.     

Bioactivities of the leaf essential oil of Curcuma longa (var. ch-66) on three species of stored-product beetles (Coleoptera)

Essential oil extracted from the leaves of turmeric, Curcuma longa L., was investigated for contact and fumigant toxicity and its effect on progeny production in three stored-product beetles, Rhyzopertha dominica F. (lesser grain borer), Sitophilus oryzae L. (rice weevil), and Tribolium castaneum Herbst (red flour beetle). Oviposition-deterrent and ovicidal actions of C. longa leaf oil were also evaluated against T. castaneum. The oil was insecticidal in both contact and fumigant toxicity assays. The adults of R. dominica were highly susceptible to contact action of C. longa leaf oil, with LD50 value of 36.71 microg/mg weight of insect, whereas in the fumigant assay, adults of S. oryzae were highly susceptible with LC50 value of 11.36 mg/liter air. Further, in T. castaneum, the C. longa oil reduced oviposition and egg hatching by 72 and 80%, respectively at the concentration of 5.2 mg/cm2. At the concentration of 40.5 mg/g food, the oil totally suppressed progeny production of all the three test insects. Nutritional indices indicate >81% antifeedant action of the oil against R. dominica, S. oryzae and T castaneum at the highest concentration tested.     

Bioactivities of l-carvone, d-carvone, and dihydrocarvone toward three stored product beetles

The rice weevil, Sitophilus oryzae L., adults were highly susceptible by contact to l-carvone, d-carvone, and dihydrocarvone when compared with the lesser grain borer, Rhyzopertha dominica F., adults and red flour beetle, Tribolium castaneum (Herbst.). Adults of R. dominica were more susceptible than the other species to fumigant vapors of l-carvone, d-carvone, and dihydrocarvone. The three larval stages (14-, 16-, and 18-d-old) of T. castaneum progressively became more susceptible with age toward contact toxicity of three test compounds but in fumigant toxicity, 16-d-old larvae of T. castaneum were more susceptible to the three compounds. Comparison of contact and fumigant toxicity of the test compounds indicates that l-carvone and d-carvone possess 24 times more fumigant toxicity toward adults of R. dominica than its contact toxicity. Overall order of toxicity was l-carvone > d-carvone > dihydrocarvone. Egg hatching and subsequent larval and adult survival of T. castaneum were significantly reduced when the eggs of T. castaneum were treated with l-carvone, d-carvone, and dihydrocarvone. l-Carvone completely suppressed egg hatching at the concentration of 7.72 mg/cm2. Data on feeding-deterrent indices indicate the high potency of l-carvone as feeding-deterrent in order of S. oryzae adults > T. castaneum adults > R. dominica adults > T. castaneum larvae.     

Effectiveness of spinosad on four classes of wheat against five stored-product insects

Spinosad is a commercial reduced-risk pesticide that is naturally derived. Spinosad's performance was evaluated on four classes of wheat (hard red winter, hard red spring, soft red winter, and durum wheats) against adults of the lesser grain borer, Rhyzopertha dominica (F.); rice weevil, Sitophilus oryzae (L.); sawtoothed grain beetle, Oryzaephilus surinamensis (L.); red flour beetle, Tribolium castaneum (Herbst); and larvae of the Indianmeal moth, Plodia interpunctella (Hübner). Beetle adults (25) or P. interpunctella eggs (50) were exposed to untreated wheat and wheat treated with spinosad at 0.1 and 1 mg (AI)/kg of grain. On all untreated wheat classes, adult beetle mortality ranged from 0 to 6%, and P. interpunctella larval mortality ranged from 10 to 19%. The effects of spinosad on R. dominica and P. interpunctella were consistent across all wheat classes. Spinosad killed all exposed R. dominica adults and significantly suppressed progeny production (84-100%) and kernel damage (66-100%) at both rates compared with untreated wheat. Spinosad was extremely effective against P. interpunctella on all wheat classes at 1 mg/kg, based on larval mortality (97.6-99.6%), suppression of egg-to-adult emergence (93-100%), and kernel damage (95-100%), relative to similar effects on untreated wheats. The effects of spinosad on S. oryzae varied among wheat classes and between spinosad rates. Spinosad was effective against S. oryzae, O. surinamensis and T. castaneun only on durum wheat at 1 mg/kg. Our results suggest spinosad to be a potential grain protectant for R. dominica and P. interpunctella management in stored wheat.     

RESISTANCE TO GRAIN PROTECTANTS AND PHOSPHINE IN COLEOPTEROUS PESTS OF GRAIN STORED ON FARMS IN NEW SOUTH WALES

Tribolium castaneum (Herbst), T. confusum Jacquelin du Val, Sitophilus oryzae (L.), S. granarius (L.), Oryzaephilus surinamensis (L.) and Rhyzopertha dominica (F.) were collected from farms in grain-growing areas in southern and northern N.S.W. and bioassayed for resistance to the grain protectants malathion, fenitrothion, bioresmethrin, carbaryl, pirimiphos-methyl, chlorpyrifos-methyl and the fumigant phosphine.
Results showed that resistance on farms in N.S.W. is similar to resistance reported elsewhere in Australia's grain handling system during the past decade. The only new resistance of practical significance was detected in O. surinamensis where 50% of populations were resistant to fenitrothion (maximum R.F. 68.4x). Fenitrothion resistance was uncommon or not detected in other species. Pirimiphos-methyl resistance was detected in 70% of O. surinamensis populations (maximum RF 44.2x) and in 1 population of T. castaneum (maximum RF 3.1x). Resistance to chlorpyrifos-methyl was detected in 39% of the O. surinamensis populations (maximum RF 8.4x), the only species tested. Low level phosphine resistance was detected in all species (maximum RF 2.2x). Malathion resistance was detected in all species except S. granarius . Resistance to bioresmethrin and carbaryl was not detected in R. dominica , the only species tested.     

Efficacy of ozone fumigation against the major grain pests in stored wheat

Field experiments were conducted in steel bins containing 13,600 kg of hard red winter wheat, Triiticum aestivum L. One bin was treated with ozone and the second bin served as a control. Stored grain insects were placed in bins for 1-, 2-, 3-, and 4-d exposure periods in sampling tubes to test ozone concentrations of 0, 25, 50, and 70 parts per million by volume (ppmv). Ozone treatments on eggs and larvae of Plodia interpunctella (Hübner) were not effective, but pupae were more susceptible. Sitophilus oryzae (L.) adults were the most susceptible species with 100% mortality reached after 2 d in all ozone treatments. However, some progeny were produced at all concentrations and exposure periods. Tribolium castaneum (Herbst) adults had 100% mortality only after 4 d at 50 or 70 ppmv. No T. castaneum progeny were produced after 2-4 d at 70 ppmv. For Rhyzopertha dominica (F.), Cryptolestes ferrugineus (Stephens), and Oryzaephilus surinamensis (L.), 100% mortality was never achieved and progeny were produced at all ozone concentrations. Laboratory experiments, testing the effectiveness of ozone in controlling psocids, were conducted in two polyvinyl chloride cylinders each containing 55 kg of hard red winter wheat. Ozone treatment at a concentration of 70 ppmv was highly effective against adult female Liposcelis bostrychophila Badonnel and Liposcelis paeta Pearman after only 1 d of exposure. However, it was not effective against eggs of both species at all exposure periods. Ozonation has potential for the control of some stored grain insect pests on wheat.     

Toxicity of propylene oxide at low pressure against life stages of four species of stored product insects

The relative toxicity of propylene oxide (PPO) at a low pressure of 100 mm Hg to four species of stored product insect at 30 degrees C over a 4-h exposure period was investigated. PPO at 100 mm Hg was toxic to all four species tested: Tribolium castaneum (Herbst), Plodia interpunctella (Hübner), Ephestia cautella (Wlk.), and Oryzaephilus surinamensis (L.). There were differences in susceptibility between the life stages of the tested insect species. Mortality tests on all life stages of the insects resulted in LD99 values ranging from 4.7 to 26.1 mg/liter. The pupal stage of E. cautella, O. surinamensis, and T. castaneum was the most tolerant stage with LD99 values of 14.4, 26.1, and 25.7 mg/liter, respectively. For P. interpunctella, the egg stage was most tolerant, with a LD99 value of 15.3 mg/liter. Generally, PPO at 100 mm Hg was more toxic to P. interpunctella and E. cautella than to O. surinamensis and T. castaneum. A 99% mortality of all life stages of the tested species was achieved at a concentrations x time product of 104.4 mg h/liter. These findings indicate that a combination of PPO with low pressure can render the fumigant a potential alternative to methyl bromide for rapid disinfestation of commodities.     

Biological activity of two juvenoids and two ecdysteroids against three stored product insects

The insecticidal activity of juvenile hormone agonists methoprene and pyriproxyfen, and the ecdysone agonists RH-5849 and tebufenozide was evaluated against susceptible and actellic-resistant strains of Tribolium castaneum and susceptible strains of Rhyzopertha dominica and Sitophilus oryzae. Concentrations ranging from 0.1 to 20 ppm of the analogues were mixed in the food medium to which the tested insects were exposed. The results showed that all these compounds could affect the development of the tested species to differing extents but had no effect on the mortality of parental adults. The two JH analogues did not prolong the life span of R. dominica and S. oryzae, but very greatly extended that of T. castaneum. The extension led to the production of giant larvae and failure to pupate. Actellic-resistant strain of T. castaneum showed some cross-resistance to methoprene and pyriproxyfen, but not to RH-5849 and tebufenozide. Pyriproxyfen was the most effective compound among the four IGRs; a concentration of 0.1 ppm could completely inhibit the F(1) adult occurrence of both S- and R-strains of T. castaneum and its LC(90)s for controlling R. dominica and S. oryzae were 0.1 and 1.2 ppm, respectively. Methoprene was highly effective against R. dominica, but less active on S. oryzae. RH-5849 could achieve almost complete control of F(1) adults of T. castaneum and R. dominica at 10 ppm, but was less potent on S. oryzae. Tebufenozide appeared to be much less active on these three species compared with the other three compounds. The percentage reductions of F(1) adults for S- and R-strains of T. castaneum at a concentration of 20 ppm were 80 and 99%, respectively.     

Effectiveness of spinosad against seven major stored-grain insects on corn

In January 2005, the United States Environmental Protection Agency registered spinosad as a stored grain protectant. No referenced data on the efficacy of spinosad on corn in suppressing major stored-grain insects have been published. In this paper, we evaluated the efficacy of spinosad against seven major stored-grain insects on shelled corn in the laboratory. Insect species tested were the red flour beetle, Tribolium castaneum （Jacquelin duVal）; rusty grain beetle, Cryptolestesferrugineus （Stephens）; lesser grain borer, Rhyzopertha dominica （F.）; sawtoothed grain beetle, Oryzaephilus surinamensis （L.）; rice weevil, Sitophilus oryzae （L.）; maize weevil, Sitophilus zeamais （Motschulsky）; and Indian meal moth, Plodia interpunctella （Htibner）. Corn kernels were treated with spinosad at 0, 0. 1, 0.5, 1, and 2 active ingredient （a.i.） mg/kg for controlling the seven species. Beetle adults or P. interpunctella eggs were introduced into each container holding 100 g of untreated or insecticide-treated corn. The seven insect species survived well on the control treatment, produced 28 to 336 progeny, and caused significant kernel damage after 49 days. On spinosad-treated corn, adult mortality of C. ferrugineus, R. dominica, 0. surinamensis, S. oryzae, and S. zeamais was 〉 98% at 1 and 2 mg/kg after 12 days. Spinosad at≥ 0.5 mg/kg completely suppressed egg-to-larval survival after 21 days and egg-to-adult emergence of P. interpunctella after 49 days, whereas 16% T. castaneum adults survived at 1 mg/kg after 12 days. Spinosad at 1 or 2 mg/kg provided complete or near complete suppression of progeny production and kernel damage of all species after 49 days. Our results indicate that spinosad at the current labeled rate of 1 mg/kg is effective against the seven stored-grain insect pests on corn.     

Synergized bifenthrin plus chlorpyrifos-methyl for control of beetles and psocids in sorghum in Australia

The efficacy of bifenthrin (0.5 mg/kg) + piperonyl butoxide (7 mg/kg) + chlorpyrifosmethyl (10 mg/kg) against beetle and psocid pests of sorghum was evaluated in silo-scale trials in southeast Queensland, Australia. The pyrethroid bifenthrin was evaluated as a potential new protectant in combination with the organophosphate chlorpyrifos-methyl, which is already registered for control of several insect pests of stored cereals. Sorghum (approximately 200 metric tons) was treated after both the 1999 and 2000 harvests and sampled at intervals to assess treatment efficacy and residue decline during up to 7 mo of storage. Generally, test strains of the beetles Rhyzopertha dominica (F.), Tribolium castaneum (Herbst), Oryzaephilus surinamensis (L), and Cryptolestes ferrugineus (Stephens) were prevented from producing live progeny for up to 7 mo. The treatment failed against one strain of R. dominica known to be resistant to bioresmethrin and organophosphates. Two malathion-resistant strains of O. surinamensis were marginally controlled with 94-100% fewer adult progeny produced. For psocids, no strains of Liposcelis bostrychophila Badonnel, Liposcelis decolor (Pearman), or Liposcelis paeta Pearman produced live progeny, although the control of a field strain of Liposcelis entomophila (Enderlein) was generally poor. Results show that this treatment should protect sorghum for at least 7 mo against most prevalent strains of grain insect in eastern Australia, although control may be limited in areas in which L. entomophila or pyrethroid-resistant R. dominica are present.     

Lethality impact of diatomaceous earth (Sayan®), bran,sawdust and clay on adult of six stored-product insects

The lethality impact of diatomaceous earth (Sayan®), bran, sawdust and clay on six major stored-product insects under laboratory conditions was determined. The lethal effects were investigated in two different methods. In the first method, experimental surface was permeated with test material, and in the second method, the test compounds were mixed with rearing mediums separately. In the first technique, adults of Sitophilus oryzae, Rhyzopertha dominica, Oryzephilus surinamensis, Lasioderma serricorne, Cryptolestes ferrugineus and Callosobruchus maculatus were placed in a Petri dish, which was contaminated with a known amount of Sayan®, bran, sawdust or clay. Mortality rate was recorded at 24, 48 and 72 h after commence of each exposure. In the second method, the lethality of test compounds which were mixed with wheat was investigated against S. oryzae, R. dominica, O. surinamensis, L. serricorne and C. ferrugineus for 1, 7 and 14 days of exposure. In the first technique, the results indicated that bran and sawdust caused more mortality rate on S. oryzae compared to Sayan®. In the second method, the results demonstrated a higher mortality caused by bran and sawdust compared to Sayan® on S. oryzae. For L. serricorne, mortality rate caused by bran and sawdust was similar, whereas, in C. maculates case, mortality rate caused by bran was more than the other treatments. The protective effects of test compounds on life span of S. oryzae and R. dominica showed significant differences among the emerged adult of insects in different treatments. In the bran treatment, S. oryzae adult's emergence was the lowest, while, in the R. dominica case, a non-significant difference among resultant number of adult insects was detected.     

Fumigation of wheat using liquid ethyl formate plus methyl isothiocyanate in 50-tonne farm bins

Australian Standard White wheat, Triticum aestivum L. (a marketing grade with mixed grain hardness),with a moisture content of 12.5% was fumigated with a new ethyl formate formulation (95% ethyl formate plus 5% methyl isothiocyanate) identified and developed by Commonwealth Scientific and Industrial Research Organization Entomology, Canberra, Australia. Wheat was fumigated with the formulation at a calculated application rate of 80 g/m3 in two 50-tonne sealed metal vertical silos located at Fisherman Islands, Queensland, Australia. Access was gained through the top of the silo where the application of the formulation was completed within a few minutes by pouring it onto the top of the wheat. After 2 h of recirculation, using a 0.5-kW fan, the in-bin concentrations of ethyl formate achieved equilibrium with a concentration variation < 7%. The ethyl formate concentration, in both silos 1 and 2, during the first day's exposure period remained above 10 g/m3. The concentration of ethyl formate by time product achieved was 790 and 650 g h/m3 in silos 1 and 2, respectively. In silo 1, the formulation was sufficient to kill all life stages of mixed age cultures of Sitophilus oryzae (L.), Rhyzopertha dominica (F.), and Tribolium castaneum (Herbst). In silo 2, control was 100% for R. dominica and T. castaneum and 99.4% for S. oryzae. After 5 d fumigation, the silo top-hatch was opened but no forced aeration was initiated. The in-bin concentration of ethyl formate was lower than the Australian experimental threshold limit value of 100 ppm. The ethyl formate and methyl isothiocyanate residues in the grain had declined to below the Australian experimental maximum residue limit of 0.2 and 0.1 mg/kg, respectively. The workspace and environmental levels of ethyl formate and methyl isothiocyanate were less than the detection limit of 0.1 ppm. The treatment with ethyl formate formulation had no affect on the wheat germination and seed color compared with untreated controls.     

Treatment of adult Coleoptera with a chitin synthesis inhibitor affects mortality and development time of their progeny

A group of insect growth regulators, the chitin synthesis inhibitors (CSIs), are being more widely used as many insects have become resistant to broad-spectrum insecticides. This study investigated the mode of action of one CSI, particularly looking at the delayed effect of treating adult insects on the survival and development of their progeny. The study describes the responses of adult stored grain beetles Sitophilus oryzae (L.) (Curculionidae) and Rhyzopertha dominica (F.) (Bostrichidae) to wheat treated with chlorfluazuron. For both species, wheat treated with chlorfluazuron reduced progeny survival at a constant rate from 1 day to 8 weeks after it had been treated. There was a direct, negative concentration-time relationship between the treatment of the adults with chlorfluazuron-treated wheat and the survival and developmental rate of their progeny. Adults that were pre-exposed for one or more weeks before oviposition to wheat treated with chlorfluazuron had significantly lower progeny survival than adults that were not pre-exposed. The EC₉₅ values were 16-fold higher for R. dominica and 40-fold higher for S. oryzae from assays without pre-exposure, than with pre-exposure. Pre-exposure of R. dominica with 0.1 mg kg^-1 and of S. oryzae with 0.4 mg kg^-1 of chlorfluazuron reduced progeny survival by 95%. Higher concentrations of chlorfluazuron produced a reduction in mean population developmental time of several days. These findings have important implications for bioassays of CSIs, since pre-exposure of the adults can significantly reduce the numbers of F1 progeny. Therefore standard bioassays may seriously underestimate the efficacy of the CSI being assayed.     

Fumigant toxicity of Eucalyptus intertexta, Eucalyptus sargentii and Eucalyptus camaldulensis against stored-product beetles

Abstract:  Three essential oils extracted from the species of the family Myrtaceae found in Kashan (centre regions of Iran) were shown to have potent fumigant toxicity against three major stored-product insects: Callosobruchus maculatus (Fab.), Sitophilus oryzae (L.) and Tribolium castaneum (Herbst). These were the essential oils from Eucalyptus intertexta R.T. Baker, Eucalyptus sargentii Maiden and Eucalyptus camaldulensis Dehnh. The mortality of 1- to 7-day-old adults of the insect pests increased with concentration from 37 to 926  μ l/l air and with exposure time from 3 to 24 h. The LC₅₀ values to the selected essential oils were between 2.55 and 3.97  μ l/l air for C. maculatus , 6.93 and 12.91  μ l/l for S. oryzae and 11.59 and 33.50  μ l/l air for T. castaneum . Fumigant effects of these essential oils were considered to warrant further research into their potential for commercial use.     

Effect of d-limonene on three stored-product beetles

d-Limonene was investigated for contact and fumigant toxicity, ovicidal effects, oviposition-deterrent, development inhibition, and feeding-deterrent activities against three stored-product beetles (Coleoptera): lesser grain borer, Rhyzopertha dominica (F.); rice weevil, Sitophilus oryzae (L.); and red flour beetle, Tribolium castaneum (Herbst). Contact and fumigant toxicity decreased as larvae aged. Contact toxicity was similar for adults of the three species tested, but R. dominica was most susceptible to fumigant activity. T. castaneum oviposition decreased as concentration of d-limonene increased and d-limonene reduced oviposition up to 92.3% at the concentration of 2.14 mg/cm2. Hatching of d-limonene-treated eggs of T. castaneum was reduced by 94.5% with no subsequent larval and adult survival at 2.14 mg/cm2 concentration. A flour disc bioassay indicated 87.7 to 96.8% feeding-deterrency by d-limonene toward all three insect species tested at the highest concentration of 60.0 mg/g food. These results suggest that d-limonene can be effectively used to suppress populations of stored-product beetles.     

Comparison of aeration and spinosad for suppressing insects in stored wheat

Field studies were conducted from July 2002 to January 2003 for evaluating the effects of controlled aeration and a commercial biological insecticide, spinosad, in suppressing insect populations in stored wheat. Six cylindrical steel bins were filled with newly harvested (2002 crop year) hard red winter wheat on 9 and 10 July 2002. Each bin contained 30.7 metric tons (1,100 bu) of wheat. Wheat in two bins was left untreated (control), whereas wheat in two bins was treated with spinosad, and in another two bins was subjected to aeration by using aeration controllers. Spinosad was applied to wheat at the time of bin filling to obtain a rate of 1 mg ([AI])/kg. Aeration controllers were set to run the fans when ambient air temperature fell below 23.9, 18.3, and 7.2 degrees C for the first, second, and third cooling cycles, respectively. We added 400 adults each of the rusty grain beetle, Cryptolestes ferrugineus (Stephens); lesser grain borer, Rhyzopertha dominica (F.); and red flour beetle, Tribolium castaneum (Herbst), to the grain at monthly intervals between July and October 2002. Insect density in the bins was estimated monthly by taking 3-kg grain samples from 21 locations within each bin by using a pneumatic grain sampler. No live T. castaneum or C. ferrugineus and very low densities of R. dominica (<0.008 adults per kilogram) were found in wheat treated with spinosad during the 6-mo sampling period. Density of C. ferrugineus and T. castaneum in aerated bins did not exceed two adults per kilogram (the Federal Grain Inspection Service standard for infested wheat), whereas R. dominica increased to 12 adults per kilogram in November 2002, which subsequently decreased to three adults per kilogram in January 2003. In the untreated (control) bins, R. dominica density increased faster than that of C. ferrugineus or T. castaneum. Density of R. dominica peaked at 58 adults per kilogram in October 2002 and decreased subsequently, whereas T. castaneum density was 10 adults per kilogram in October 2002 but increased to 78 adults per kilogram in January 2003. Density of C. ferrugineus increased steadily during the 6-mo study period and was highest (six adults per kilogram) in January 2003. This is the first report comparing the field efficacy of spinosad and aeration in managing insects in farm bins. Our results suggest that spinosad is very effective in suppressing R. dominica, C. ferrugineus, and T. castaneum populations in stored wheat.     

Hole density and capture of stored-product insect pests in grain probe traps

The relationship between number of holes in a grain probe trap body and capture of stored-grain pests was determined in laboratory tests using adults of the rice weevil, Sitophilus oryzae (L.), the sawtoothed grain beetle, Oryzaephilus surinamensis (L.), and the red flour beetle, Tribolium castaneum (Herbst). Polyvinylchloride (PVC) probe bodies were attached to electronic sensor heads, and insect captures were recorded electronically using an Electronic Grain Probe Insect Counter (EGPIC) system. In comparisons among PVC probe trap bodies with 60-492 holes, tested at 71 insects per kg in 2.8 kg of soft wheat in cylindrical mini-silos, sawtoothed grain beetle and rice weevil captures were directly related to number of holes in the probe trap body, but there was no relationship for red flour beetle capture. Subsequent tests were conducted comparing sawtoothed grain beetle and rice weevil captures in a PVC probe body with 210 holes over a 40-cm long trapping surface with two commercially available probe traps, a polycarbonate (Lexan) probe trap with 180 holes over a 14-cm long trapping surface and a polyethylene (WBII) probe trap with 750 holes over a 34-cm long trapping surface. The highest percentage capture of both species was in the WBII probe trap, but the 210-hole PVC probe body was as effective as the Lexan probe body for rice weevils and sawtoothed grain beetles at 71 and 17 insects per kg of wheat, respectively.     

Immediate and delayed mortality of Rhyzopertha dominica (Coleoptera: Bostrichidae) and Sitophilus oryzae (Coleoptera: Curculionidae) adults exposed to spinosad-treated commodities

A series of tests was conducted to characterize differences in the mortality of the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), and rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae), exposed to three commodities treated with a liquid and dry spinosad formulation. In laboratory bioassays, adults of the two insect species were exposed to untreated wheat, Triticum aestivum L., corn, Zea mays L., and sorghum, Sorghum bicolor (L.) Moench., and to commodities treated with 1 mg (AI)/kg of liquid and dry spinosad formulations. Mortality was assessed from independent samples examined at specific time intervals to determine immediate mortality and after 24 h of recovery on untreated grain at 28 degrees C and 65% RH to determine delayed mortality. Comparison of the time required for 50% (LT50) and 95% (LT95) mortality indicated that R. dominica adults were consistently and significantly more susceptible (died quickly) than S. oryzae adults when exposed to spinosad-treated commodities. In general, the toxicity of liquid and dry spinosad formulations was similar against R. dominica or S. oryzae. The toxicity of spinosad to each species varied slightly among the three commodities, and there were no consistent trends to suggest that spinosad was more effective on one commodity versus another. LT50 values based on immediate mortality for R. dominica on all commodities ranged from 0.45 to 0.74 d; corresponding values based on delayed mortality ranged from 0.04 to 0.23 d, suggesting delayed toxic action of spinosad in R. dominica. LT50 values based on immediate and delayed mortality for S. oryzae on all three commodities treated with the two spinosad formulations were essentially similar and ranged from 2.75 to 4.56 d. LT95 values for R. dominica based on immediate mortality on spinosad-treated commodities ranged from 1.75 to 3.36 d, and those based on delayed mortality ranged from 0.49 to 1.88 d. There were no significant differences in LT95 values based on immediate and delayed mortality for S. oryzae on spinosad-treated commodities, and the LT95 values ranged from 7.62 to 18.87 d. The toxicity of spinosad was enhanced during a 24-h holding period after removal from spinosad-treated commodities only against R. dominica adults, and possible reasons for increased postexposure mortality of R. dominica adults after brief exposures to spinosad warrant further study.     

1%蛇床子素粉剂对三种储粮害虫的防效

研究1%蛇床子素粉剂在5种处理浓度下对3种储粮害虫谷蠹Rhizopertha dominica Fabricius、玉米象Sitophilus zeamai Motschulsky和赤拟谷盗Tribolium castaneum Herbst的防治效果。室内毒力测定结果表明,按有效成分0.5mg/kg(蛇床子素:粮食)浓度处理粮食,7d后,粮食中谷蠹、玉米象和赤拟谷盗的校正死亡率分别为97.78%、100%、86.70%,防治效果优于对照药剂防虫磷和谷虫净。将药剂处理4个月后的粮食进行接虫试验,15d后谷蠹和玉米象的防治效果仍可达100%,达到储粮害虫防治要求。     

Susceptibility of laboratory and field strains of four stored-product insect species to spinosad

Two field strains of the Indianmeal moth, Plodia interpunctella (Hübner); red flour beetle, Tribolium castaneum (Herbst); and lesser grain borer, Rhyzopertha dominica (F.), and one field strain of the rusty grain beetle, Cryptolestes ferrugineus (Stephens), were collected from hard red winter wheat stored on farms in northeastern Kansas. Fifty eggs of P. interpunctella and 25 beetle adults of each species were exposed to 100 g of untreated wheat or wheat treated with various rates of spinosad, to determine susceptibility of the field and corresponding insecticide-susceptible laboratory strains. Mortality of beetle adults and P. interpunctella larvae was assessed after 7 and 21 d postinfestation, respectively. Field strains of P. interpunctella, C. ferrugineus, and T. castaneum were less susceptible to spinosad than the corresponding laboratory strains. The LD50 and LD95 values for P. interpunctella and C. ferrugineus field strains were 1.7-2.5 times greater than values for corresponding laboratory strains. Adults of both laboratory and field strains of T. castaneum were tolerant to spinosad, resulting in <88% mortality at 8 mg/kg. The LD50 and LD95 values for the field strains of T. castaneum were 2.0-7.5 times greater compared with similar values for the laboratory strain. The field and laboratory strains of R. dominica were highly susceptible to spinosad, and one of the field strains was relatively less susceptible to spinosad than the laboratory strain. Our results confirm a range of biological variability in field populations, which is consistent with findings for other compounds, and underscores the need to adopt resistance management programs with stored grain insect pests. The baseline data generated on the susceptibility of the four insect species to spinosad will be useful for monitoring resistance development and for setting field rates.