Primulina curvituba sp. nov. (Gesneriaceae) from a limestone area in Guangxi,China

Primulina curvituba B. Pan, L.H. Yang & M. Kang, a new species of Primulina (Gesneriaceae) from Guangxi, China, is described and illustrated. This new species is mainly diagnosed by its strongly curved corolla tube, which is a rare character in Primulina. The morphological relationship and differences between this species and its related congeners are discussed. The conservation status of P. curvituba is assessed as ‘Critically Endangered’ (CR) according to IUCN.     

Primulina minor sp. nov. (Gesneriaceae) from a limestone cave in Hunan,China

Primulina minor from the Daoxian county, Hunan province in China is described. This new species is similar to Primulina jingxiensis (Yan Liu, W. B. Xu & H. S. Gao) W. B. Xu & K. F. Chung and P. longzhouensis (B. Pan & W. H. Wu) W. B. Xu & K. F. Chung, but differs by its number of leaves (4–6), broadly ovate, orbicular‐ovate or orbicular leaf blade, 2–4 cymes, short peduncle (1.8–2.0 cm long), white (or white with light purple tinge) corolla, small corolla (8–10 mm long), 3 staminoides of which the lateral ones are short (0.75–0.90 mm long) and adnate to 1.6–1.7 mm above the corolla base.     

Primulina guangxiensis sp. nov. (Gesneriaceae) from a karst cave in Guangxi,China

Primulina guangxiensis Yan Liu & W. B. Xu, a new species of Gesneriaceae from Guangxi, China, is described and illustrated. The new species is related to Primulina tabacum Hance, but differs in the leaf blade being obliquely ovate to obliquely oblong‐ovate, 2.5–6.0×1.3–4.3 cm, eith margin entire to repand and base inequilateral, petiole slimly terete, 1.5–6.0 cm long, ca 2–3 mm across, cymes 1–2‐branched, 1–5‐flowered and corolla tube hypocrateriform, 20–25 mm long, 2 mm across. The new species is rare, currently known only from one site in a karst cave in western Guangxi.     

Primulina suichuanensis sp. nov. (Gesneriaceae) from Danxia landform in Jiangxi,China

Primulina suichuanensis X. L. Yu & J. J. Zhou, a new species of Gesneriaceae from Jiangxi, China, is described and illustrated. Morphologically, the new species is most similar to P. roseoalba, but differs by entire leaf blade margin, narrowly ovate bracts, reddish–purple or bluish–purple corolla, 3.0–3.4 cm long corolla tube, white filaments, and 3.6 cm long pistil. It flowers from September to November.     

Primulina melanofilamenta sp. nov. (Gesneriaceae) from Guangxi,China

A new species of Gesneriaceae, Primulina melanofilamenta Ying Liu & F. Wen from Guangxi, China, is illustrated and described. It is morphologically distinct from the most similar species P. eburnea and P. atroglandulosa by the color of the corolla, dark purplish black filaments, anthers backed with a beard of white hairs, the length of staminodes and the hairs of styles. The somatic chromosome number of P. melanofilamenta is also reported as 2n = 36.     

Selective spore germination on shoots of Homalothecium lutescens,a moss with dwarf males

Spores from three bryophyte species with dwarf males (Homalothecium lutescens, Homalothecium sericeum and Isothecium alopecuroides) were sown on shoots of H. lutescens in vitro. After 10 months, presence and fertility of dwarf plants were scored. Spores of the more distantly related I. alopecuroides were unable to develop into dwarf plants on H. lutescens. Spores of both H. lutescens and H. sericeum developed into dwarf plants. In fact, dwarf plants of H. sericeum were both more abundant and more often fertile than those of H. lutescens. The ability of H. sericeum spores to develop into dwarf males on shoots of H. lutescens suggests a possible pathway for hybridization between the two species. On the other hand, the inability of I. alopecuroides to develop into dwarf males on shoots of H. lutescens suggests that regulation of spore germination and dwarf male development on host shoots is associated with the degree of relatedness between species.     

Primulina jianghuaensis sp. nov. (Gesneriaceae) from a limestone cave in southern Hunan,China

A new species of Gesneriaceae, Primulina jianghuaensis K. M. Liu & X. Z. Cai, from a limestone cave in Hunan Province, China, is identified and described. Its seed and pollen grain morphology are described. The new species is morphologically closely related to P. lingchuanensis ( =Chiritopsis lingchuanensis) and P. danxiaensis ( =Chiritopsis danxiaensis), but it can be distinguished by its 12–25 (–30) leaves, ovate or broad ovate leaf blade, subcordate, truncate or slightly decurrent leaf base, undulate leaf margin, 5–20 cymes, and 2–3 staminodes.     

Primulina debaoensis sp. nov. (Gesneriaceae) from a limestone cave in Guangxi,China

A new species of Gesneriaceae, Primulina debaoensis Neng Jiang & Hong Li, from Guangxi Zhuangzu Autonomous Region, China, is illustrated and described here. It resembles another endemic and stenochoric species in Guangxi, P. pungentisepala (W. T. Wang) Mich. Möller & A. Weber, but differs by having 12–18 or more leaves, leaf blade being broadly linear, oblanceolate‐elliptical or oblanceolate, bracts being ovate or elliptical with silvery or argenteous nerves and by having a yellow ovary that is 4–5 mm long and sparsely white pubescent.     

Primulina maciejewskii sp. nov. (Gesneriaceae) from Guangdong,China

Primulina maciejewskii F. Wen, R.L. Zhang & A.Q. Dong, a new species of Primulina (Gesneriaceae) from Guangdong, China, is described and illustrated. It is similar to P. lobulata and P. danxiaensis in leaf and flower shape, but can be distinguished by bract shape, peduncle, pedicel, and bract size, filament and anther indumentum, and number of bracts, staminodes and stigma.     

广西石灰岩地区苦苣苔科植物一新种——北流报春苣苔

报道了广西石灰岩地区苦苣苔科报春苣苔属(Primulina Hance)1新种——北流报春苣苔(P. beiliuensis B. Pan & S. X. Huang)。该新种在形态上与黄花牛耳朵[P. lutea(Yan Liu & Y. G. Wei)Mich. Möller & A. Weber]较近,但叶宽卵形,叶基部近心形,叶缘具浅钝齿或呈浅波状齿,花冠紫色,花冠、花序梗、花梗、苞片及花萼均被紫色短柔毛而区别与后者; 分子生物学证据表明,在系统发育上与桂林小花苣苔[P. repanda var. guilinensis(W. T. Wang)Mich. Möller & A. Weber]近缘,但两者在形态学上相差较远。     

中国丝瓜藓属直蒴组的分类学研究

采用经典分类学研究方法,对河北师范大学植物标本馆(HBNU)等国内多个标本馆保存的中国丝瓜藓属直蒴组(Pohlia Hedw.section Cacodon Lindb.ex Broth.)及其近缘类群植物标本共计130余号进行了分类学分析鉴定,并对所确定的中国产直蒴组物种的芽胞大小、形状及叶原基数量和长度等形态学特征进行比较分析,以期为中国丝瓜藓属直蒴组的分类学研究提供理论依据。结果表明:(1)经鉴定中国丝瓜藓属直蒴组植物共有8种——夭命丝瓜藓[Pohlia annotina(Hedw.)Lindb.]、糙枝丝瓜藓[P.camptotrachela(Ren.Card.)Broth.]、林地丝瓜藓[P.drummondii(Müll.Hal.)Andr.]、疣齿丝瓜藓[P.flexuosa Harv.]、纤毛丝瓜藓[P.hisae T.J.Kop.et J.X.Luo]、异芽丝瓜藓[P.leucostoma(BoschSande Lac.)M.Fleisch.]、念珠丝瓜藓[P.lutescens(Limpr.)H.Lindb.]和卵蒴丝瓜藓[P.proligera(Kindb.ex Breidl.)Lindb.ex Arnell]。(2)中国丝瓜藓属直蒴组植物的重要鉴别特征是在假根或叶腋处生有芽胞,而且芽胞形状以及叶原基形状和数量在直蒴组中存在差异,如念珠丝瓜藓具有假根生芽胞,而疣齿丝瓜藓具有两型芽胞。(3)对所鉴定的中国丝瓜藓属直蒴组8种植物的识别特征、生境及其与相近种的关系进行了详细描述,并绘制了形态特征墨线图。     

Primulina elegans (Gesneriaceae), a new species from North Vietnam

Primulina elegans (Gesneriaceae), a new species from Vietnam is described. This species is similar to P. gemella and P. diffusa in having stolons and papillose-hispid leaves, but is easily distinguished from them by having 9–15 cymes and corollas with two brown stripes on the adaxial lip and nine purplish lines on the abaxial lip. Furthermore, Primulina elegans differs from P. gemmella by its broadly infundibuliform corollas with purplish glandular pubescent filaments, and from P. diffusa by having three slightly purplish glandular pubescent staminodes.     

Primulina tsoongii sp. nov. (Gesneriaceae) from a limestone area in north Guangxi,China

Primulina tsoongii sp. nov. (Gesneriaceae) is described and illustrated here. This new species resembles P. hochiensis and P. hochiensis var. rosulata, but is easily distinguished by its sparsely hirsute mature leaves, narrow bracts (only 0.4–0.5 in diameter) and undivided yellowish ligulate stigma with dense elongate papillae.     

念珠丝瓜藓(缺齿藓科)形态特征及其在中国的新分布

通过对中国产丝瓜藓属Pohlia(缺齿藓科Mielichhoferiaceae)植物系统地分类学研究,对本属一具有假根生念珠状排列的芽胞的种——念珠丝瓜藓的形态解剖学特征和地理分布进行了修订,并讨论了念珠丝瓜藓的生境,以及与其相近种泛生丝瓜藓、明齿丝瓜藓和黄丝瓜藓等进行了比较分析。根据标本信息,确定念珠丝瓜藓在中国的分布由原来的5省区扩大到10省区,并绘制了该种在中国的分布图。     

Suitability of four palm species for the development of the invasive pest Brontispa longissima (Coleoptera: Chrysomelidae) in the field

The coconut hispine beetle Brontispa longissima (Gestro) supposedly originated in Indonesia and Papua New Guinea. It is a serious invasive pest of the coconut palm Cocos nucifera L. in Southeast and East Asia. In Japan, it has established itself using Satakentia liukiuensis (Hatushima) H.E. Moore as a main host on Ishigaki and Iriomote Islands where C. nucifera is rare. To assess the probability of further establishment of B. longissima in novel habitats where C. nucifera and S. liukiuensis are not available, we examined the suitability of four common palm species in Japan for oviposition and immature development of B. longissima: Chrysalidocarpus lutescens (Bory) H. Wendl., Phoenix roebelenii O'Brien, S. liukiuensis and Washingtonia filifera (Linden ex André) H. Wendl. When seedlings of the four palm species were placed in pots in an experimental field on Ishigaki Island, all four species were inhabited and infested by wild B. longissima adults. Oviposition and immature development were observed on P. roebelenii and S. liukiuensis but not on C. lutescens and W. filifera. When field‐collected adults were released into mesh bags enclosing the potted seedlings, they oviposited on all four species. The eggs developed into adults on P. roebelenii, S. liukiuensis and W. filifera. On C. lutescens, however, hatched larvae died during the first or second instar.     

Primulina xiziae sp. nov. (Gesneriaceae) from Zhejiang Province,China

A new species of Gesneriaceae, Primulina xiziae Fang Wen, Yue Wang & G. J. Hua, from Zhejiang Province, China, is identified, illustrated and described. It resembles the widespread P. eburnea, and the stenochoric P. pseudoeburnea, but differs by the leaf blades being ovate‐elliptic or ovate, chartaceous with ciliate margins, 8.5–13.8 mm long, slender peduncle, ovate, ca 1.2 × 1.0 cm large bracts that are generally withered but persistent before full‐blooming stage, slender tubular‐infundibuliform, ca 25.0 × 7.5 mm corolla tube, limb lobes with acute apices, 3 staminodes, and by forming a dormancy bud in winter.     

广西植物名录补遗(Ⅷ)

该文报道了17个广西新记录物种，即柔毛阴山荠[Yinshania henryi(Oliv.) Y. H. Zhang]、臭荠(Lepidium didymum L.)、小酸模(Rumexa acetosella L.)、浙江凤仙花(Impatiens chekiangensis Y. L. Chen)、贵州海桐(Pittosporum kweichowense Gowda)、越南圆叶梭罗树(Reevesia orbicularis Tardieu)、冬青叶鼠刺(Itea ilicifolia Oliv.)、窄叶枇杷(Eriobotrya henryi Nakai)、瘤枝密花树[Myrsine verruculosa(C. Chen) Pipoly&C. Chen]、毛腺萼木(Mycetia hirta Hutch.)、二萼丰花草[Spermacoce exilis(L. O. Williams) C. D. Adams]、滇苦菜(Picris divaricata Vaniot)、纤细通泉草(Mazus gracilis Hemsl.)、皱叶荚蒾(Viburnum rhytid...     

Primulina petrocosmeoides sp. nov. (Gesneriaceae) from Guangxi,China

Primulina petrocosmeoides Bo Pan & Fang Wen sp. nov. (Gesneriaceae) from Guangxi, China, is described and illustrated. This new species is similar to P. weii Mich. Möller & A. Weber, but differs from it in leaf blade ovate to elliptical, 1.0 × 0.8 to 2.5 × 2.0 cm, leaf base broadly cuneate, cymes 5–16, 2–6‐flowered, bracts narrowly lanceolate, calyx lobes lanceolate, 4.0–6.5 mm long, corolla bluish purple, 1.2–1.5 cm long, pubescent outside but glabrous inside, filaments purple, pubescent, staminodes 3, stigma trapezoid with its apex lobed to the middle and with dense short papillae.     

Chromosome 9 of Ellobius lutescens is the X chromosome

Ellobius lutescens carries an apparently identical karyotype (2n = 17) in both sexes. On the basis of indirect evidence the unpaired chromosome 9 has been considered to represent the X chromosome of this species. We have obtained data to substantiate this view by four different techniques. After fusion of HPRT^– RAG cells with E. lutescens fibroblasts we demonstrated that the enzymes HPRT and G6PD are localized on the presumptive X chromosome. By analysis of pachytene figures after silver staining we showed by electron microscopy that the single chromosome exhibits the typical features of an X chromosome in male meiosis. Hybridization of (GATA)₄ and (GACA)₄ oligonucleotide probes to E. lutescens DNA revealed several distinct bands in the high molecular weight range some of which appeared to be specific for the individual but not for the sex of the animal. Hybridization in situ of the (GATA)₄ probe on metaphase spreads of E. lutescens did not highlight any particular chromosome segment but showed a significant deficit of these sequences in chromosome 9. These observations are discussed with respect to their bearing on X chromosome determination. Finally it is concluded that E. lutescens should be an ideal tool for testing candidate genes assumed to be involved in primary sex determination.     

Nitrogen and carbon contents and δ15N and δ13C signatures in six bryophyte species: assessment of long‐term deposition changes (1980–2010) in Spanish beech forests

In this study we used recent (2010) and herbarium material (1980) of six bryophyte species to assess long‐term atmospheric deposition in natural forested areas in northern Spain. For this purpose, tissue nitrogen and carbon content, as well as δ¹³C and δ¹⁵N signatures of samples of Hypnum cupressiforme, Polytrichastrum formosum, Leucobryum juniperoideum, Rhytidiadelphus loreus, Homalothecium lutescens and Diplophyllum albicans were analysed and comparisons made between years and species. In addition, the usefulness of each of the six species was evaluated. The range of values observed was similar to that in other studies carried out in rural areas. Significantly lower values were found in 2010 for N (H. cupressiforme), δ¹⁵N (R. loreus and D. albicans), C (R. loreus) and δ¹³C (all except L. juniperoideum). Our natural areas are thus now less influenced by atmospheric pollutants than they were, most probably due to changes in some traditional local activities. Differences were observed between species for all the four parameters studied, so different species must not be analysed together. Finally, R. loreus and H. lutescens seem to be good bioindicators, sensitive even with a few samples, although further studies are needed to corroborate their usefulness.