共查询到20条相似文献，搜索用时 520 毫秒
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Han Feng Wei Liu Da-Cheng Wang 《Acta Crystallographica. Section F, Structural Biology Communications》2016,72(4):294-299
Cells respond to various proteotoxic stimuli and maintain protein homeostasis through a conserved mechanism called the heat‐shock response, which is characterized by the enhanced synthesis of heat‐shock proteins. This response is mediated by heat‐shock factors (HSFs). Four genes encoding HSF1–HSF4 exist in the genome of mammals. In this protein family, HSF1 is the orthologue of the single HSF in lower eukaryotic organisms and is the major regulator of the heat‐shock response, while HSF2, which shows low sequence homology to HSF1, serves as a developmental regulator. Increasing evidence has revealed biochemical properties and functional roles that are unique to HSF2, such as its DNA‐binding preference and sumoylation patterns, which are distinct from those of HSF1. The structural basis for such differences, however, is poorly understood owing to the lack of available mammalian HSF structures. The N‐terminal DNA‐binding domain (DBD) is the most conserved functional module and is the only crystallizable domain in HSFs. To date, only HSF1 homologue structures from yeast and fruit fly have been determined. Along with extensive studies of the HSF family, more structural information, particularly from members with a remoter phylogenic relationship to the reported structures, e.g. HSF2, is needed in order to better understand the detailed mechanisms of HSF biology. In this work, the recombinant DBD (residues 7–112) from human HSF2 was produced in Escherichia coli and crystallized. An X‐ray diffraction data set was collected to 1.32 Å resolution from a crystal belonging to space group P212121 with unit cell‐parameters a = 65.66, b = 67.26, c = 93.25 Å. The data‐evaluation statistics revealed good quality of the collected data, thus establishing a solid basis for the determination of the first structure at atomic resolution in this protein family. 相似文献
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Suzuki N Sejima H Tam R Schlauch K Mittler R 《The Plant journal : for cell and molecular biology》2011,66(5):844-851
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Interleukin enhancer binding factor (ILF) binds to the interleukin-2 (IL-2) promoter and regulates IL-2 gene expression. In this study, the 3D structure of the DNA-binding domain of ILF was determined by multidimensional NMR spectroscopy. NMR structure analysis revealed that the DNA-binding domain of ILF is a new member of the winged helix/forkhead family, and that its wing 2 contains an extra alpha-helix. This is the first study to report the presence of a C-terminal alpha-helix in place of a typical wing 2 in a member of this family. This structural difference may be responsible for the different DNA-binding specificity of ILF compared to other winged helix/forkhead proteins. Our deletion studies of the fragments of ILF also suggest that the C-terminal region plays a regulatory role in DNA binding. 相似文献
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Gong‐Hong Wei Gwenael Badis Michael F Berger Teemu Kivioja Kimmo Palin Martin Enge Martin Bonke Arttu Jolma Markku Varjosalo Andrew R Gehrke Jian Yan Shaheynoor Talukder Mikko Turunen Mikko Taipale Hendrik G Stunnenberg Esko Ukkonen Timothy R Hughes Martha L Bulyk Jussi Taipale 《The EMBO journal》2010,29(13):2147-2160
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Gaohua Liu Yuanpeng J. Huang Rong Xiao Dongyan Wang Thomas B. Acton Gaetano T. Montelione 《Proteins》2010,78(9):2170-2175
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热激转录因子(HSFs)参与了植物生长发育的调控以及多种非生物胁迫适应基因的表达调控。HSFs通常形成同源三聚体,激活转录活性从而发挥功能。本文综述了热激转录因子的基本结构、亚细胞定位、转录调控、功能多样性及其在植物适应极端温度、盐害、干旱、强光和氧化胁迫等非生物胁迫过程中的作用。HSFs是提高高等植物抗多重胁迫的优质候选基因,对其深入研究具有重要的应用价值。未来,通过生物基因工程等手段利用HSFs提高各类作物抗性具有广阔的发展前景。 相似文献
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Both geldanamycin (GA) and radicicol (RA) are HSP90 binding agents that possess antitumour activities. Although the in vitro data indicated that the inhibitory constant of RA is much bigger than that of GA, the in vivo data on drug efficacy might reveal different results. We have recently shown that treatment with GA induces a heat-shock response and that calcium mobilization may be involved in the process. By using induction of HSP70 as the endpoint assay, we found changes in upstream signaling mediators, including HSF1 and calcium mobilization, as well as possible involvement of protein kinase in human non-small cell lung cancer H460 cells treated with GA and RA. Our results demonstrated that calcium mobilization, a calcium dependent and H7-sensitive protein kinase, along with HSF1 activation by phosphorylation, are all involved in the HSP70 induction process triggered by the drugs. However, only GA, but not RA, can provoke a rapid calcium mobilization and thereby result in an instant induction of HSP70. Furthermore, the rapid calcium influx, followed by instant HSP induction, could be achieved in GA- or RA-treated cells placed in a medium containing excessive calcium while the response was completely abolished in cells depleted of calcium. Taken together, our findings suggest that differential calcium signaling may account for the differential induction of HSP and the action of GA and RA. 相似文献