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1.
Michael Kruse Sally P. Leys Isabel M. Müller Werner E.G. Müller 《Journal of molecular evolution》1998,46(6):721-728
Recent analyses of genes encoding proteins typical for multicellularity, especially adhesion molecules and receptors, favor
the conclusion that all metazoan phyla, including the phylum Porifera (sponges), are of monophyletic origin. However, none
of these data includes cDNA encoding a protein from the sponge class Hexactinellida. We have now isolated and characterized
the cDNA encoding a protein kinase C, belonging to the C subfamily (cPKC), from the hexactinellid sponge Rhabdocalyptus dawsoni. The two conserved regions, the regulatory part with the pseudosubstrate site, the two zinc fingers, and the C2 domain, as
well as the catalytic domain were used for phylogenetic analyses. Sequence alignment and construction of a phylogenetic tree
from the catalytic domains revealed that the yeast Saccharomyces cerevisiae and the protozoan Trypanosoma brucei are at the base of the tree, while the hexactinellid R. dawsoni branches off first among the metazoan sequences; the other two classes of the Porifera, the Calcarea (the sequence from Sycon raphanus was used) and the Demospongiae (sequences from Geodia cydonium and Suberites domuncula were used), branch off later. The statistically robust tree also shows that the two cPKC sequences from the higher invertebrates
Drosophila melanogaster and Lytechinus pictus are most closely related to the calcareous sponge. This finding was also confirmed by comparing the regulatory part of the
kinase gene. We suggest, that (i) within the phylum Porifera, the class Hexactinellida diverged first from a common ancestor
to the Calcarea and the Demospongiae, which both appeared later, and (ii) the higher invertebrates are more closely related
to the calcareous sponges.
Received: 6 August 1997 / Accepted: 24 October 1997 相似文献
2.
Porifera (sponges) represent the most ancient, extant metazoan phylum. They existed already prior to the 'Cambrian Explosion'. Based on the analysis of aa sequences of informative proteins, it is highly likely that all metazoan phyla evolved from only one common ancestor (monophyletic origin). As 'autapomorphic' proteins which are restricted to Metazoa only, integrin receptors, receptors with scavenger receptor cysteine-rich repeats, neuronal-like receptors and protein-tyrosine kinases (PTKs) have been identified in Porifera. From the marine sponge Geodia cydonium, a receptor tyrosine kinase (RTK) has been cloned that comprises the characteristic structural topology known from other metazoan RTKs; an extracellular domain, the transmembrane region, the juxtamembrane region and the TK domain. Only two introns, within the coding region of the RTK gene, could be found, which separate the two highly polymorphic immunoglobulin-like domains, found in the extracellular region of the enzyme. The functional role of this sponge RTK could be demonstrated both in situ (grafting experiments) and in vitro (increase of intracellular Ca2+ level). Upstream of this RTK gene, two further genes coding for tyrosine kinases (TK) have been identified. Both are intron-free. The deduced aa sequence of the first gene shows no transmembrane segment; from the second gene--so far--only half of its catalytic domain is known. A phylogenetic analysis with the TK domains from these sequences and a fourth, from a novel scavenger RTK (all domains comprise the signature for the TK class II receptors), showed that they are distantly related to the insulin and insulin-like receptors. The presented findings support the 'introns-late' hypothesis for such genes that encode 'metazoan' proteins. It is proposed that the TKs evolved from protein-serine/threonine kinases through modularization and subsequent exon shuffling. After formation of the ancestral TKs, the modules lost the framing introns to protect the evolutionary novelty. Since cell culture systems of sponges are now available, it can be expected that soon also those mechanisms that control the developmental programs will be unravelled. 相似文献
3.
Michael Kruse Vera Gamulin Helena Cetkovic Zeev Pancer Isabel M. Müller Werner E. G. Müller 《Journal of molecular evolution》1996,43(4):374-383
Protein kinases C (PKCs) comprise closely related Ser/Thr kinases, ubiquitously present in animal tissues; they respond to
second messengers, e.g., Ca2+ and/or diacylglycerol, to express their activities. Two PKCs have been sequenced from Geodia cydonium, a member of the lowest multicellular animals, the sponges (Porifera). One sponge G. cydonium PKC, GCPKC1, belongs to the ``novel' (Ca2+-independent) PKC (nPKC) subfamily while the second one, GCPKC2, has the hallmarks of the ``conventional' (Ca2+-dependent) PKC (cPKC) subfamily. The alignment of the Ser/Thr catalytic kinase domains, of the predicted aa sequences for
these cDNAs with respective segments from previously reported sequences, revealed highest homology to PKCs from animals but
also distant relationships to Ser/Thr kinases from protozoa, plants, and bacteria. However, a comparison of the complete structures
of the sponge PKCs, which are—already—identical to those of nPKCs and cPKCs from higher metazoa, with the structures of protozoan,
plant, and bacterial Ser/Thr kinases indicates that the metazoan PKCs have to be distinguished from the nonmetazoan enzymes.
These data indicate that metazoan PKCs have a universal common ancestor which they share with the nonmetazoan Ser/Thr kinases
with respect to the kinase domain, but they differ from them in overall structural composition.
Received: 10 January 1996 / Accepted: 12 March 1996 相似文献
4.
Suga H Koyanagi M Hoshiyama D Ono K Iwabe N Kuma K Miyata T 《Journal of molecular evolution》1999,48(6):646-653
To know whether genes involved in cell–cell communication typical of multicellular animals dramatically increased in concert
with the Cambrian explosion, the rapid evolutionary burst in the major groups of animals, and whether these genes exist in
the sponge lacking cell cohesiveness and coordination typical of eumetazoans, we have carried out cloning of the G-protein
α subunit (Gα) and the protein tyrosine kinase (PTK) cDNAs from Ephydatia fluviatilis (freshwater sponge) and Hydra magnipapillata strain 105 (hydra). We obtained 13 Gα and 20 PTK cDNAs. Generally animal gene families diverged first by gene duplication
(subtype duplication) that gave rise to diverse subtypes with different primary functions, followed by further gene duplication
in the same subtype (isoform duplication) that gave rise to isoform genes with virtually identical function. Phylogenetic
trees of Gα and PTK families including cDNAs from sponge and hydra revealed that most of the present-day subtypes had been
established in the very early evolution of animals before the parazoan–eumetazoan split, the earliest branching among the
extant animal phyla, by extensive subtype duplication: for PTK and Gα families, 23 and 9 subtype duplications were observed
in the early stage before the parazoan–eumetazoan split, respectively, and after that split, only 2 and 1 subtype duplications
were found, respectively. After the separation from arthropods, vertebrates underwent frequent isoform duplications before
the fish–tetrapod split. Furthermore, rapid amino acid changes appear to have occurred in concert with the extensive subtype
duplication and isoform duplication. Thus the pattern of gene diversification during animal evolution might be characterized
by bursts of gene duplication interrupted by considerably long periods of silence, instead of proceeding gradually, and there
might be no direct link between the Cambrian explosion and the extensive gene duplication that generated diverse functions
(subtypes) of these families.
Received: 4 November 1998 / Accepted: 17 November 1998 相似文献
5.
Joachim Schütze Alexander Skorokhod Isabel M. Müller Werner E.G. Müller 《Journal of molecular evolution》2001,53(4-5):402-415
One crucial event during evolution to multicellularity was the development of either direct cell–cell contact or indirect
interaction via extracellular matrix (ECM) molecules. The identification of those polypeptides provides conclusive data on
the phylogenetic relationship of metazoan phyla and helps us to understand the position of the Metazoa among the other kingdoms.
Recently it became evident that the ECM of sponges is amazingly complex; it is composed of fibrous molecules, e.g., collagen,
and their corresponding receptors, which are highly similar to those existing in other metazoan phyla. While these data already
support the view of monophyly of Metazoa, additional studies are required to understand whether these molecules, which are
similar in their primary sequence, also have the same function throughout the metazoan kingdom. In the present study we identified
the ligand for one of the autopomorphic characters of Metazoa, the single-transmembrane receptor protein with the receptor
tyrosine kinase (RTK) from G. cydonium, as an example: the putative mucus-like protein from G. cydonium. This protein was upregulated during autograft fusion in the homologous system with kinetics similar to those of the RTK.
Additionally, a cDNA was isolated from S. domuncula whose deduced polypeptide displays a high sequence similarity to dermatopontin, an ECM molecule found exclusively in Metazoa.
Furthermore, it is documented that expression of the fibrous ECM molecule collagen is regulated by the characteristic metazoan
morphogens myotrophin and endothelial monocyte-activating polypeptide. These data indicate that the ECM of sponges is not
an unstructured ground substance but provides the basis for integrated cell communication.
Received: 26 October 2000 / Accepted: 1 February 2001 相似文献
6.
Bruton tyrosine kinase-like protein, BtkSD, is present in the marine sponge Suberites domuncula 总被引:1,自引:0,他引:1
Sponges, the simplest and most ancient phylum of Metazoa, encode in their genome complex and highly sophisticated proteins that evolved together with multicellularity and are found only in metazoan animals. We report here the finding of a Bruton tyrosine kinase (BTK)-like protein in the marine sponge Suberites domuncula (Demospongiae). The nucleotide sequence of one sponge cDNA predicts a 700-aa-long protein, which contains all of the characteristic domains for the Tec family of protein tyrosine kinases (PTKs). The highest homology (38% identity, 55% overall similarity) was found with human BTK and TEC PTKs. Sponge PTK was therefore named BtkSD. Human BTK is involved in the maturation of B cells and mutations in the BTK gene cause X-linked agammaglobulinemia. Kinases from the Tec family are not present in Caenorhabditis elegans and, until now, they were found only in insects and higher animal taxa. Our finding implies that the BTK/TEC genes are of a very ancient origin. 相似文献
7.
Five cDNAs (pDidact2–pDidact6), representing different actin genes, were isolated from a Diphyllobothrium dendriticum cDNA library, and the DNA as well as the putative amino acid sequences were determined. The corresponding Didact2 and Didact4 genes code for peptides 376 amino acids long, with molecular weights 41,772 and 41,744 Da, respectively, while the deduced
Didact3 protein is 377 amino acids long and weighs 41,912 Da. The pDidact5 and -6 cDNAs lack nucleotides corresponding to three to six amino acids at the amino-terminus. Two of the five cDNAs contain the
conventional AATAAA as the putative polyadenylation signal, one has the common variant ATTAAA, whereas the hexanucleotide
AATAGA is found 15 and 18 nucleotides, respectively, upstream of the poly(A) site in two of the cDNAs. Phylogenetic studies
including 102 actin protein sequences revealed that there are at least four different types of cestode actins. In this study
three of these types were found to be expressed in the adult D. dendriticum tapeworm. Structurally the cestode actin groupings differ from each other to an extent seen only among the metazoan actins
between the vertebrate muscle and cytoplasmic isoforms. In the phylogenetic trees constructed, cestode actins were seen to
map to two different regions, one on the border of the metazoan actins and the other within this group. It is, however, difficult
to say whether the cestode actins branched off early in the metazoan evolution or if this position in the phylogenetic tree
only reflects upon differences in evolutionary rate.
Received: 19 June 1996 / Accepted: 20 August 1996 相似文献
8.
Ferritin, a protein widespread in nature, concentrates iron ∼1011–1012-fold above the solubility within a spherical shell of 24 subunits; it derives in plants and animals from a common ancestor
(based on sequence) but displays a cytoplasmic location in animals compared to the plastid in contemporary plants. Ferritin
gene regulation in plants and animals is altered by development, hormones, and excess iron; iron signals target DNA in plants
but mRNA in animals. Evolution has thus conserved the two end points of ferritin gene expression, the physiological signals
and the protein structure, while allowing some divergence of the genetic mechanisms. Comparison of ferritin gene organization
in plants and animals, made possible by the cloning of a dicot (soybean) ferritin gene presented here and the recent cloning
of two monocot (maize) ferritin genes, shows evolutionary divergence in ferritin gene organization between plants and animals
but conservation among plants or among animals; divergence in the genetic mechanism for iron regulation is reflected by the
absence in all three plant genes of the IRE, a highly conserved, noncoding sequence in vertebrate animal ferritin mRNA. In
plant ferritin genes, the number of introns (n= 7) is higher than in animals (n= 3). Second, no intron positions are conserved when ferritin genes of plants and animals are compared, although all ferritin
gene introns are in the coding region; within kingdoms, the intron positions in ferritin genes are conserved. Finally, secondary
protein structure has no apparent relationship to intron/exon boundaries in plant ferritin genes, whereas in animal ferritin
genes the correspondence is high. The structural differences in introns/exons among phylogenetically related ferritin coding
sequences and the high conservation of the gene structure within plant or animal kingdoms suggest that kingdom-specific functional
constraints may exist to maintain a particular intron/exon pattern within ferritin genes. In the case of plants, where ferritin
gene intron placement is unrelated to triplet codons or protein structure, and where ferritin is targeted to the plastid,
the selection pressure on gene organization may relate to RNA function and plastid/nuclear signaling.
Received: 25 July 1995 / Accepted: 3 October 1995 相似文献
9.
Molecular evolution of calmodulin-like domain protein kinases (CDPKs) in plants and protists 总被引:1,自引:0,他引:1
Many genes for calmodulin-like domain protein kinases (CDPKs) have been identified in plants and Alveolate protists. To study
the molecular evolution of the CDPK gene family, we performed a phylogenetic analysis of CDPK genomic sequences. Analysis
of introns supports the phylogenetic analysis; CDPK genes with similar intron/exon structure are grouped together on the phylogenetic
tree. Conserved introns support a monophyletic origin for plant CDPKs, CDPK-related kinases, and phosphoenolpyruvate carboxylase
kinases. Plant CDPKs divide into two major branches. Plant CDPK genes on one branch share common intron positions with protist
CDPK genes. The introns shared between protist and plant CDPKs presumably originated before the divergence of plants from
Alveolates. Additionally, the calmodulin-like domains of protist CDPKs have intron positions in common with animal and fungal
calmodulin genes. These results, together with the presence of a highly conserved phase zero intron located precisely at the
beginning of the calmodulin-like domain, suggest that the ancestral CDPK gene could have originated from the fusion of protein
kinase and calmodulin genes facilitated by recombination of ancient introns.
Received: 11 July 2000 / Accepted: 18 April 2001 相似文献
10.
Protein tyrosine phosphatases (PTPs) regulate various physiological events in animal cells. They comprise a diverse family
which are classified into two categories, receptor type and nonreceptor type. From the domain organization and phylogenetic
tree, we have classified known PTPs into 17 subtypes (9 receptor-type and 8 nonreceptor-type PTPs) which are characterized
by different organization of functional domain and independent cluster in tree. The receptor type PTPs are thought to be implicated
in cell–cell adhesion by association of cell adhesion molecules. Since sponges are the most primitive multicellular animals
and are thought to be lacking cell cohesiveness and coordination typical of eumetazoans, cloning and sequencing of PTP cDNAs
of Ephydatia fluviatilis (freshwater sponge) have been conducted by RT-PCR to determine whether or not sponges have PTP genes in their genomes. We
have isolated nine PTPs, of which five are possibly receptor type. A phylogenetic tree including the sponge PTPs revealed
that most of the gene duplications that gave rise to the 17 subtypes had been completed in the very early evolution of animals
before the parazoan–eumetazoan split, the earliest branching among extant animal phyla. The family tree also revealed the
rapid evolutionary rate of PTP subtypes in the early stage of animal evolution.
Received: 22 October 1998 / Accepted: 27 November 1998 相似文献
11.
We have determined the genomic structure of an integrin β-subunit gene from the coral, Acropora millepora. The coding region of the gene contains 26 introns, spaced relatively uniformly, and this is significantly more than have
been found in any integrin β-subunit genes from higher animals. Twenty-five of the 26 coral introns are also found in a β-subunit
gene from at least one other phylum, indicating that the coral introns are ancestral. While there are some suggestions of
intron gain or sliding, the predominant theme seen in the homologues from higher animals is extensive intron loss. The coral
baseline allows one to infer that a number of introns found in only one phylum of higher animals result from frequent intron
loss, as opposed to the seemingly more parsimonious alternative of isolated intron gain. The patterns of intron loss confirm
results from protein sequences that most of the vertebrate genes, with the exception of β4, belong to one of two β subunit
families. The similarity of the patterns within each of the β1,2,7 and β3,5,6,8 groups indicates that these gene structures
have been very stable since early vertebrate evolution. Intron loss has been more extensive in the invertebrate genes, and
obvious patterns have yet to emerge in this more limited data set.
Received: 5 March 2001 / Accepted: 17 May 2001 相似文献
12.
Characterization of the Hydra Lamin and Its Gene: A Molecular Phylogeny of Metazoan Lamins 总被引:4,自引:0,他引:4
Andreas Erber Dieter Riemer Helmut Hofemeister Marc Bovenschulte Reimer Stick Georgia Panopoulou Hans Lehrach Klaus Weber 《Journal of molecular evolution》1999,49(2):260-271
We report sequences for nuclear lamins from the teleost fish Danio and six invertebrates. These include two cnidarians (Hydra and Tealia), one priapulid, two echinoderms, and the cephalochordate Branchiostoma. Combining these results with earlier data on Drosophila, Caenorhabditis elegans, and various vertebrates, the following conclusions on lamin evolution can be drawn. First, all invertebrate lamins resemble
in size the vertebrate B-type lamin. Second, all lamins described previously for amphibia, birds and mammals as well as the
first lamin of a fish, characterized here, show a cluster of 7 to 12 acidic residues in the tail domain. Since this acidic
cluster is absent from all invertebrate lamins including that of the cephalochordate Branchiostoma, it was acquired with the vertebrate lineage. The larger A-type lamin of differentiated cells must have arisen subsequently
by gene duplication and insertion of an extra exon. This extra exon of the vertebrate A-lamins is the only major change in
domain organization in metazoan lamin evolution. Third, the three introns of the Hydra and Priapulus genes correspond in position to the last three introns of vertebrate B-type lamin genes. Thus the entirely different gene
organization of the C. elegans and Drosophila Dmo genes seems to reflect evolutionary drift, which probably also accounts for the fact that C. elegans has the most diverse lamin sequence. Finally we discuss the possibility that two lamin types, a constitutively expressed
one and a developmentally regulated one, arose independently on the arthropod and vertebrate lineages.
Received: 4 February 1999 / Accepted: 1 April 1999 相似文献
13.
François Agnès Marguerite-Marie Toux Catherine André Francis Galibert 《Journal of molecular evolution》1997,45(1):43-49
Receptor tyrosine kinases with five, seven, and three Ig-like domains in their extracellular region are grouped in subclasses
IIIa, IIIb, and IIIc, respectively. Here, we describe the genomic organization of the extracellular coding region of the human FGFR4 (IIIc) and FLT4 (IIIb) genes and compare it to that of the human FGFR1(IIIc), KIT, and FMS (IIIa). The results show that while genes belonging to the same subclass have an identical exon/intron structure in their extracellular
coding region—as they do in their intracellular coding region—genes of related subclasses only have a similar exon/intron
structure. These results strongly support the hypothesis that the genes of the three subclasses evolved from a common ancestor
by duplications involving entire genes, already in pieces. Hypotheses on the origin of introns and on the difference in the
number of extracellular Ig-like domains in the three gene subclasses are discussed.
Received: 19 August 1996 / Accepted: 2 January 1997 相似文献
14.
15.
Alessandra Gambacurta Maria Cristina Piro Franca Ascoli 《Journal of molecular evolution》1998,47(2):167-171
Vertebrate and many invertebrate globin genes have a three-exon/two-intron organization, with introns in highly conserved
positions. According to the ``intron early' hypothesis, introns are the vestigial segments which flank previously independent
coding sequences, thus providing evidence for the assembly of the ancient proteins by ``exon shuffling.' In this paper, we
report the analysis of the genes of the bivalve mollusk Scapharca inaequivalvis tetrameric hemoglobin (HbII), which support this hypothesis, at least for the hemoglobin genes. We show the existence of
``minigenes' in the IIA and IIB globin genes, spanning part of the first and second introns, ``in frame' with the heme-binding
domain coded by the second exon. Further support for the exon shuffling hypothesis can be found in the degree of identity
of the ``new' translated sequences with those flanking the central protein domain of some invertebrate hemoglobins.
Received: 31 July 1997 / Accepted: 12 December 1997 相似文献
16.
The sequences of the entire blue opsin gene in the squirrel monkey (Saimiri boliviensis) and the five introns of the human blue opsin gene were obtained. Intron 3 of these genes contains an Alu sequence and intron
4 contains a partial mer13 sequence. A comparison of the squirrel monkey opsin sequence with published mammalian opsin sequences
shows that features believed to be functionally critical are all conserved. However, the blue opsin has evolved twice as fast
as rhodopsin and is only as conservative as the β globin, which has evolved at the average rate of mammalian proteins. Interestingly,
the interhelical loops are, on average, actually more conservative than the transmembrane α helical regions. The introns of
the blue opsin gene have evolved at the average rate of introns in primate genes.
Received: 5 August 1996 / Accepted: 2 October 1996 相似文献
17.
Sponges (phylum Porifera) are the phylogenetic oldest Metazoa still extant. They can be considered as reference animals (Urmetazoa) for the understanding of the evolutionary processes resulting in the creation of Metazoa in general and also for the metazoan gene organization in particular. In the marine sponge Suberites domuncula, genes encoding p38 and JNK kinases contain nine and twelve introns, respectively. Eight introns in both genes share the same positions and the identical phases. One p38 intron slipped for six bases and the JNK gene has three more introns. However, the sequences of the introns are not conserved and the introns in JNK gene are generally much longer. Introns interrupt most of the conserved kinase subdomains I-XI and are found in all three phases (0, 1 and 2). We analyzed in details p38 and JNK genes from human, Caenorhabditis elegans and Drosophila melanogaster and found in most genes introns at the positions identical to those in sponge genes. The exceptions are two p38 genes from D. melanogaster that have lost all introns in the coding sequence. The positions of 11 introns in each of four human p38 genes are fully conserved and ten introns occupy identical positions as the introns in sponge p38 or JNK genes. The same is true for nine, out of ten introns in the human JNK-1 gene. The introns in human p38 and JNK genes are on average more than ten times longer than corresponding introns in sponges. It was proposed that yeast HOG1-like kinases (from i.e. Saccharomyces cerevisiae and Emericella nidulans) and metazoan p38 and JNK kinases are orthologues. p38 and JNK genes were created after the split from fungi by the duplication and diversification of the HOG1-like progenitor gene. Our results further support the common origin of p38 and JNK genes and speak in favor of a very early time of duplication. The ancestral gene contained at least ten introns, which are still present at the very conserved positions in p38 and JNK genes of extant animals. Four of these introns are present at the same positions in the HOG-like gene in the fungus E. nidulans. The others probably entered the ancestral gene after the split of fungi, but before the duplication of the gene and before the creation of the common, urmetazoan progenitor of all multicellular animals. A second gene coding for an immune molecule is described, the allograft inflammatory factor, which likewise showed a highly conserved exon/intron structure in S. domuncula and in human. These data show that the intron/exon borders are highly conserved in genes from sponges to human. 相似文献
18.
Nicole Rebscher Christina Deichmann Stefanie Sudhop Jens Holger Fritzenwanker Stephen Green Monika Hassel 《Development genes and evolution》2009,219(9-10):455-468
We have analyzed the evolution of fibroblast growth factor receptor (FGFR) tyrosine kinase genes throughout a wide range of animal phyla. No evidence for an FGFR gene was found in Porifera, but we tentatively identified an FGFR gene in the placozoan Trichoplax adhaerens. The gene encodes a protein with three immunoglobulin-like domains, a single-pass transmembrane, and a split tyrosine kinase domain. By superimposing intron positions of 20 FGFR genes from Placozoa, Cnidaria, Protostomia, and Deuterostomia over the respective protein domain structure, we identified ten ancestral introns and three conserved intron groups. Our analysis shows (1) that the position of ancestral introns correlates to the modular structure of FGFRs, (2) that the acidic domain very likely evolved in the last common ancestor of triploblasts, (3) that splicing of IgIII was enabled by a triploblast-specific insertion, and (4) that IgI is subject to substantial loss or duplication particularly in quickly evolving genomes. Moreover, intron positions in the catalytic domain of FGFRs map to the borders of protein subdomains highly conserved in other serine/threonine kinases. Nevertheless, these introns were introduced in metazoan receptor tyrosine kinases exclusively. Our data support the view that protein evolution dating back to the Cambrian explosion took place in such a short time window that only subtle changes in the domain structure are detectable in extant representatives of animal phyla. We propose that the first multidomain FGFR originated in the last common ancestor of Placozoa, Cnidaria, and Bilateria. Additional domains were introduced mainly in the ancestor of triploblasts and in the Ecdysozoa. 相似文献
19.
M.K. Tan 《Journal of molecular evolution》1997,44(6):637-645
Studies of the distribution of the three group I introns (intron A, intron T, and intron AT) in the 26S rDNA of Gaeumannomyces graminis had suggested that they were transferred to a common ancestor of G. graminis var. avenae and var. tritici after it had branched off from var. graminis. Intron AT and intron A exhibited vertical inheritance and coevolved in concert with their hosts. Intron loss could occur
after its acquisition. Loss of any one of the three introns could occur in var. tritici whereas only loss of intron T had been found in the majority of var. avenae isolates. The existence of isolates of var. tritici and var. avenae with three introns suggested that intron loss could be reversed by intron acquisition and that the whole process is a dynamic
one. This process of intron acquisition and intron loss reached different equilibrium points for different varieties and subgroups,
which explained the irregular distribution of these introns in G. graminis. Each of the three group I introns was more closely related to other intron sequences that share the same insertion point
in the 26S rDNA than to each other. These introns in distantly related organisms appeared to have a common ancestry. This
system had provided a good model for studies on both the lateral transfer and common ancestry of group I introns in the 26S
rRNA genes.
Received: 17 May 1996 / Accepted: 14 January 1997 相似文献
20.
In the unicellular green alga, Chlamydomonas reinhardtii, cytochrome oxidase subunit 2 (cox2) and 3 (cox3) genes are missing from the mitochondrial genome. We isolated and sequenced a BAC clone that carries the whole cox3 gene and its corresponding cDNA. Almost the entire cox2 gene and its cDNA were also determined. Comparison of the genomic and the corresponding cDNA sequences revealed that the
cox3 gene contains as many as nine spliceosomal introns and that cox2 bears six introns. Putative mitochondria targeting signals were predicted at each N terminal of the cox genes. These spliceosomal introns were typical GT–AG-type introns, which are very common not only in Chlamydomonas nuclear genes but also in diverse eukaryotic taxa. We found no particular distinguishing features in the cox introns. Comparative analysis of these genes with the various mitochondrial genes showed that 8 of the 15 introns were interrupting
the conserved mature protein coding segments, while the other 7 introns were located in the N-terminal target peptide regions.
Phylogenetic analysis of the evolutionary position of C. reinhardtii in Chlorophyta was carried out and the existence of the cox2 and cox3 genes in the mitochondrial genome was superimposed in the tree. This analysis clearly shows that these cox genes were relocated during the evolution of Chlorophyceae. It is apparent that long before the estimated period of relocation
of these mitochondrial genes, the cytosol had lost the splicing ability for group II introns. Therefore, at least eight introns
located in the mature protein coding region cannot be the direct descendant of group II introns. Here, we conclude that the
presence of these introns is due to the invasion of spliceosomal introns, which occurred during the evolution of Chlorophyceae.
This finding provides concrete evidence supporting the ``intron-late' model, which rests largely on the mobility of spliceosomal
introns.
Received: 22 August 2000 / Accepted: 28 February 2001 相似文献