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Binding sites of HeLa cell nuclear proteins on the upstream region of adenovirus type 5 E1A gene. 总被引:6,自引:3,他引:3 下载免费PDF全文
Twenty one binding sites of HeLa cell nuclear proteins were identified on the upstream region of adenovirus type 5 E1A gene using DNase I footprint assay. The proximal promoter region contained five binding sites that overlapped the cap site, TATA box, TATA-like sequence, CCAAT box, and -100 region relative to the E1A cap site(+1). The -190 region was a potential site for octamer-motif binding proteins, such as NFIII and OBP100. An upstream copy of the E1A enhancer element 1 was the site for a factor (E1A-F) with the binding specificity of XGGAYGT (X = A, C; Y = A, T). E1A-F factor also bound to three other sites, one of which coincided with the distal E1A enhancer element. The distal element also contained a potential site for ATF factor. The adenovirus minimal origin of DNA replication competed for DNA-protein complex formation on the CCAAT and TATA box region and the -190 region, suggesting that these regions interacted with a common or related factor. 相似文献
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Adenovirus-2 E1a and E1b gene products regulate enhancer mediated transcription 总被引:10,自引:0,他引:10 下载免费PDF全文
V Natarajan 《Nucleic acids research》1986,14(23):9445-9456
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The Epstein-Barr virus (EBV) early promoter DR contains a cis-acting element responsive to the EBV transactivator EB1 and an enhancer with constitutive and inducible activities. 总被引:19,自引:15,他引:4 下载免费PDF全文
P Chavrier H Gruffat A Chevallier-Greco M Buisson A Sergeant 《Journal of virology》1989,63(2):607-614
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