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1.
The hypotheses of this study were (i) that shovelnose sturgeon would make upstream movements to spawn, (ii) movement of spawning fish would be greater in a year with higher discharge, and (iii) that spawning fish would have greater movements than reproductively inactive fish. Shovelnose sturgeon Scaphirhynchus platorynchus (Rafinesque, 1820) in five reproductive categories (e.g. males, confirmed spawning females, potentially spawning females, atretic females, and reproductively inactive females) were tracked in 2008 and 2009. All reproductive categories, except reproductively inactive females, exhibited large‐scale movements and had omnidirectional movements. No differences in movement rates were observed in confirmed spawning females between years despite a 45% higher peak discharge in 2008 (839 m3 s?1) than in 2009 (578 m3 s?1). A peak discharge was obtained at a faster rate in 2008 (165 m3 s?1 day?1) than in 2009 (39 m3 s?1 day?1), and high discharge was of greater duration in 2008. Reproductively inactive females did not exhibit large‐scale movements and their movement rate differed from other reproductive categories. Shovelnose sturgeon spawned in both years, despite highly varying hydrographs between years.  相似文献   

2.
The main purpose of this study was to gather swimming performance information for two endemic cyprinids of the Iberian Peninsula to contribute to the optimization of fish ways. Critical swimming speed (Ucrit) was determined for the Tagus nase Pseudochondrostoma polylepis (Steindachner, 1864) and for the bordallo Squalius carolitertii (Doadrio, 1988) in a swimming tunnel. From a total of 80 P. polylepis tested, the mean (± SD) Ucrit observed was 0.78 ± 0.15 ms?1 (c. 3.74 ± 0.93 BL s?1); the 68 S. carolitertii tested presented an Ucrit of 0.54 ± 0.1 ms?1 (c. 4.43 ± 0.74 BL s?1). Significant interspecific differences were found between the Ucrit of the tested cyprinids. Intraspecific comparisons between the Ucrit and the variables of size, sex, condition factor and gonado‐somatic index were also made. No sex‐or gonad maturation‐related differences between the Ucrit were identified, but the robust P. polylepis were found to be stronger swimmers. Water velocities in fish ways for P. polylepis and S. carolitertii should aim, on average, for lower than 0.7 and 0.5 ms?1, respectively.  相似文献   

3.
Adult zebrafish Danio rerio were exposed to an electric shock of 3 V and 1A for 5 s delivered by field backpack electrofishing gear, to induce a taxis followed by a narcosis. The effect of such electric shock was investigated on both the individual performances (swimming capacities and costs of transport) and at cellular and mitochondrial levels (oxygen consumption and oxidative balance). The observed survival rate was very high (96·8%) independent of swimming speed (up to 10 body length s?1). The results showed no effect of the treatment on the metabolism and cost of transport of the fish. Nor did the electroshock trigger any changes on muscular oxidative balance and bioenergetics even if red muscle fibres were more oxidative than white muscle. Phosphorylating respiration rates rose between (mean 1 s.e. ) 11·16 ± 1·36 pmol O2 s?1 mg?1 and 15·63 ± 1·60 pmol O2 s?1 mg?1 for red muscle fibres whereas phosphorylating respiration rates only reached 8·73 ± 1·27 pmol O2 s?1 mg?1 in white muscle. Such an absence of detectable physiological consequences after electro‐induced narcosis both at organismal and cellular scales indicate that this capture method has no apparent negative post‐shock performance under the conditions of this study.  相似文献   

4.
The anaethetic effects of 2‐phenoxyethanol (2‐PE) on possible primary (cortisol level) and secondary (hematological indices and glucose level) stress responses were studied in Persian sturgeon (Acipenser persicus). Fish were first exposed to 0.1, 0.3, 0.5, 0.7, 0.9 and 1.1 ml L?1 2‐PE, and the time to induction (deep anaethesia) and recovery were measured. At a concentration of 0.1 ml L?1, 2‐PE failed to induce deep anaethesia in fish, whereas at concentrations of 0.7, 0.9 and 1.1 ml L?1 all fish were anaethetized within 3 min of exposure. For assessing possible stress effects caused by effective concentrations of 2‐PE, the hematological indices, serum cortisol and glucose were determined in the deeply anaethetized fish as stress indicators. The 2‐PE exposure resulted in significant increases in red blood cell (RBC) values at 0.3 and 0.5 ml L?1; parallel increases in hemoglobin values were also observed at these concentrations (P < 0.01). Moreover, a lower concentration of 2‐PE (0.3 ml L?1) caused a significant increase in hematocrit values (P < 0.05). Among the hematological indices, mean corpuscular volume (MCV) values decreased at 0.5 ml L?1 when compared with the control and other groups (P < 0.05). Serum cortisol level was elevated at 0.3, 0.5 and 0.7 concentrations of 2‐PE (P < 0.01). The glucose level followed a trend similar to that observed for cortisol. The outcome of these experiments shows that 2‐PE at a concentration of 0.9 ml L?1 is a suitable anaesthetic for Persian sturgeon. This study demonstrates that rapid induction of deep anaethesia with a relatively high concentration of 2‐PE (0.9 and 1.1 ml L?1) was associated with the lowest effects on signs of physiological stress in Persian sturgeon.  相似文献   

5.
Atlantic sturgeon Acipenser oxyrinchus surfacing behaviour was investigated in Minas Basin (45° 20′ N; 64° 00′ W) and the Bay of Fundy with pop‐up satellite archival tags (MiniPAT) measuring physical variables (pressure, temperature, light ) . Of six tags deployed during June and July, five provided pop‐up locations and two were recovered after c. 4 months. Analysis of recovered archival data revealed that the frequency of surfacing events was highest (78·9%) when A. oxyrinchus were in Minas Basin at depths <10 m. Surfacing frequency decreased substantially when fish migrated into greater depths of the Bay of Fundy (>40 m). The tidal cycle in Minas Basin had a significant relationship to surfacing frequency, with the most surfacing events (49·5%) occurring on the flood tide, from mid‐ to high‐tide. Surfacing events ranged from 0–12 a day and the maximum number occurred between 2300 and 0300 hours. Maximum surfacing ascent speeds ranged from 0·50 to 4·17 m s?1 and maximum descent speeds ranged from 0·17 to 3·17 m s?1. Buoyancy control, by gulping air to inflate the gas bladder, is proposed as the main reason for surfacing behaviour in A. oxyrinchus.  相似文献   

6.
The swimming performance of juvenile shortnose sturgeon (~16 cm TL, ~20 g), Acipenser brevirostrum, was quantified with regards to temperature (5 to 25°C) using both increased (Ucrit) and fixed velocity (endurance) tests in a laboratory setting. Sturgeons were found to show reduced Ucrit values at 5 and 10°C (25.99 and 28.86 cm s?1 respectively), with performance beginning to plateau at 15°C through 25°C (33.99 cm s?1). For the endurance protocol, fish were tested at speeds of 35, 40 and 45 cm s?1 at 5, 15 and 25°C. Performance within a single speed was similar at all temperatures, indicating the usage of anaerobic metabolism to fuel locomotion at these higher velocities. Overall, shortnose sturgeon demonstrated high tolerance towards a wide range of temperatures but showed few differences between performance levels at colder or warmer water conditions.  相似文献   

7.
The swimming performance and associated swimming behaviour (i.e. substratum‐skimming, station‐holding and free swimming) were assessed in shortnose sturgeon Acipenser brevirostrum during critical swimming and endurance swimming tests over a rough and a smooth substratum. It was hypothesized that the addition of a rough substratum in the swimming flume may provide a surface for the A. brevirostrum to grip and offer an energetic advantage. Substratum type did not affect the critical swimming performance, but A. brevirostrum consistently performed more bottom behaviours (i.e. substratum‐skimming and station‐holding) while on a smooth substratum. Acipenser brevirostrum had little contact with the rough substratum until the velocity was >1 body length s?1. Endurance swimming time was significantly lower for A. brevirostrum over the rough bottom at the highest velocity (30 cm s?1) which may be attributed to the observed increase in free swimming and decrease in bottom behaviours. During endurance swimming, the rough substratum was mainly used at intermediate velocities, suggesting that there may be a stability cost associated with being in contact with the rough substratum at certain velocities.  相似文献   

8.
Juvenile rainbow trout Oncorhynchus mykiss were exposed to two concentrations each of 17β‐oestradiol (E2; natural oestrogen hormone) or 17α‐ethinyl oestradiol (EE2; a potent synthetic oestrogen hormone) to evaluate their potential effects on burst‐swimming performance. In each of six successive burst‐swimming assays, burst‐swimming speed (Uburst) was lower in fish exposed to 0·5 and 1 µg l?1 E2 and EE2 for four days compared with control fish. A practice swim (2 days prior to exposure initiation) in control fish elevated initial Uburst values, but this training effect was not evident in the 1 µg l?1 EE2‐exposed fish. Several potential oestrogen‐mediated mechanisms for Uburst reductions were investigated, including effects on metabolic products, osmoregulation and blood oxygen‐carrying capacity. Prior to burst‐swimming trials, fish exposed to E2 and EE2 for 4 days had significantly reduced erythrocyte numbers and lower plasma glucose concentrations. After six repeated burst‐swimming trials, plasma glucose, lactate and creatinine concentrations were not significantly different among treatment groups; however, plasma Cl? concentrations were significantly reduced in E2‐ and EE2‐treated fish. In summary, E2 and EE2 exposure altered oxygen‐carrying capacity ([erythrocytes]) and an osmoregulatory‐related variable ([Cl?]), effects that may underlie reductions in burst‐swimming speed, which will have implications for fish performance in the wild.  相似文献   

9.
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11.
Changes in the critical swimming speed (Ucrit, cm s?1) with ontogeny of 2·5–12·5 month‐old juvenile anadromous Chinese sturgeon Acipenser sinesis were measured in a modified Blazka‐type swimming tunnel. The absolute Ucrit increased with length, mass and age; the relative Ucrit (body lengths, s?1), however, decreased. Juvenile A. sinesis did not display a parr–smolt transformation at the length or age threshold to tolerate full‐strength seawater.  相似文献   

12.
The aim of this study was to determine the suitability of water quality in the Roanoke River of North Carolina for supporting shortnose sturgeon Acipenser brevirostrum, an endangered species in the United States. Fathead minnows Pimephales promelas were also evaluated alongside the sturgeon as a comparative species to measure potential differences in fish survival, growth, contaminant accumulation, and histopathology in a 28‐day in situ toxicity test. Captively propagated juvenile shortnose sturgeon (total length 49 ± 8 mm, mean ± SD) and fathead minnows (total length 39 ± 3 mm, mean ± SD) were used in the test and their outcomes were compared to simultaneous measurements of water quality (temperature, dissolved oxygen, pH, conductivity, total ammonia nitrogen, hardness, alkalinity, turbidity) and contaminant chemistry (metals, polycyclic aromatic hydrocarbons, organochlorine pesticides, current use pesticides, polychlorinated biphenyls) in river water and sediment. In the in situ test, there were three non‐riverine control sites and eight riverine test sites with three replicate cages (25 × 15‐cm (OD) clear plexiglass with 200‐μm tear‐resistant Nitex® screen over each end) of 20 shortnose sturgeon per cage at each site. There was a single cage of fathead minnows also deployed at each site alongside the sturgeon cages. Survival of caged shortnose sturgeon among the riverine sites averaged 9% (range 1.7–25%) on day 22 of the 28‐day study, whereas sturgeon survival at the non‐riverine control sites averaged 64% (range 33–98%). In contrast to sturgeon, only one riverine deployed fathead minnow died (average 99.4% survival) over the 28‐day test period and none of the control fathead minnows died. Although chemical analyses revealed the presence of retene (7‐isopropyl‐1‐methylphenanthrene), a pulp and paper mill derived compound with known dioxin‐like toxicity to early life stages of fish, in significant quantities in the water (251–603 ng L?1) and sediment (up to 5000 ng g?1 dry weight) at several river sites, no correlation was detected of adverse water quality conditions or measured contaminant concentrations to the poor survival of sturgeon among riverine test sites. Histopathology analysis determined that the mortality of the river deployed shortnose sturgeon was likely due to liver and kidney lesions from an unknown agent(s). Given the poor survival of shortnose sturgeon (9%) and high survival of fathead minnows (99.4%) at the riverine test sites, our study indicates that conditions in the Roanoke River are incongruous with the needs of juvenile shortnose sturgeon and that fathead minnows, commonly used standard toxicity test organisms, do not adequately predict the sensitivity of shortnose sturgeon. Therefore, additional research is needed to help identify specific limiting factors and management actions for the enhancement and recovery of this imperiled fish species.  相似文献   

13.
This study focused on the acute physiological responses to saltwater exposure in juvenile shortnose sturgeon Acipenser brevirostrum. In two separate laboratory experiments, 2 year‐old A. brevirostrum were exposed to either full (32) or half‐strength (16) seawater for up to 24 h. First, oxygen consumption rates were used to estimate the metabolic costs over 24 h. Secondly, blood and muscle samples were analysed at 6, 12 and 24 h for water loss, various measures of osmoregulatory status (plasma osmolality and ions) and other standard haematological variables. Juveniles exposed to full‐strength seawater showed significant decreases in oxygen consumption rates during the 24 h exposure. Furthermore, seawater‐exposed fish had significantly increased plasma osmolality, ions (Na+ and Cl?) and a 17% decrease in total wet mass over the 24 h exposure period. To a lesser extent, increases in osmolality, ions and mass loss were observed in fish exposed to half‐strength seawater but no changes to oxygen consumption. Cortisol was also significantly increased in fish exposed to full‐strength seawater. While plasma protein was elevated following 24 h in full‐strength seawater, haemoglobin, haematocrit and plasma glucose levels did not change with increased salinity. These results imply an inability of juvenile A. brevirostrum to regulate water and ions in full‐strength seawater within 24 h. Nonetheless, no mortality occurred in any exposure, suggesting that juvenile A. brevirostrum can tolerate short periods in saline environments.  相似文献   

14.
The critical thermal maximum (CTmax) and the associated hematological response of juvenile (~145 g, n = 8 for both species) Atlantic Acipenser oxyrinchus and shortnose Acipenser brevirostrum sturgeons acclimated to 15°C were determined using a heating rate of 8°C h?1. The critical thermal maximum averaged 30.8°C and 31.6°C for Atlantic and shortnose sturgeon, respectively, and values fell within the range noted for other sturgeon species. Oxygen‐carrying capacity (hemoglobin and hematocrit) measures were generally unaffected by thermal stress. Plasma lactate levels increased from 0.5 mm to 4 mm following temperature stress in both species. Both plasma glucose and potassium levels increased following CTmax, however, these levels were about double in the shortnose sturgeon. Lastly, plasma sodium and chloride levels were significantly depressed (by more than 10%) following thermal stress in shortnose sturgeon, whereas only chloride levels decreased in Atlantic sturgeon. Taken together, while CTmax values were similar, thermal stress resulted in different hematological profiles; these differences are consistent when compared to other stressors, and may be related to the phylogenetic position and thus could reflect the evolutionary history of these two species.  相似文献   

15.
In the past decade improved acoustic hard‐ and software have enabled estimations of abundance and distribution patterns of aquatic organism, including non‐intrusive monitoring of fish migrations and behaviour. In this study, a high frequency acoustic camera (DIDSON‐LR, 1.2 MHz, 0.7 MHz) and a portable split‐beam scientific echo sounder (Simrad EY60, 200 kHz) collected acoustic data on 192 and 157 individuals within 24 hr (19–20 April 2011) in the Mituo reach of the Yangtze River, China. Mean fish length estimated from the acoustic camera data was 18.7 ± 5.6 cm, with an average swimming speed of 0.19 ± 0.13 m s?1. The mean fish target strength (TS) produced by the echo sounder was ?43.8 ± 4.4 dB, which corresponded to 5.7–119.9 cm fish length when converted by three different TS‐length equations. Average swimming speed was 0.11 ± 0.06 m s?1 from the echo sounder. Compared with the actual fish catch by the three layers of drift gill net in the survey area, the target length indicated by DIDSON was more accurate than the EY60 results, which were highly affected by the choice of TS length equations. It was determined that the two devices used synchronously could estimate fish length effectively to investigate their behaviour and distribution.  相似文献   

16.
The objectives of this study were to compare the physicochemical properties of coelomic fluid (CF) in three sturgeon species, sterlet sturgeon Acipenser ruthenus (age 5–8 years), Siberian sturgeon Acipenser baerii (age 15–20 years), and Russian sturgeon Acipenser gueldenstaedtii (age 13–18 years). For the study, CF was collected by plastic pipette from the eggs of mature female sterlet sturgeon (N = 10), Siberian sturgeon (N = 7) and Russian sturgeon (N = 4); osmolality, pH, ionic composition (Na+, K+, Ca2+, Mg2+, Cl?), glucose, cholesterol and total protein of the CF were then analyzed. Mean concentration of K+ (4.4–6.11 mmol/L), Cl? (89.8–98.0 mmol/L), Ca2+ (0.87–0.96 mmol/L), Mg2+ (0.57–0.67 mmol/L), cholesterol (0.13–0.16 mmol/L), total protein (2.41–3.57 g/L), pH (7.92–7.98), and osmolality (190–213 mOsm/kg) of CF were not significantly different among these three species. However, the mean concentration of Na+ was significantly lower in sterlet sturgeon (104.6 ± 7.7 mmol/L; p < .05) than in the Siberian (126.4 ± 6.2) and Russian (123.0 ± 5.9) sturgeon. For these three species, Na+, K+, Cl?, and Mg2+ were the dominating ions; several significant correlations were observed among these ions and other physiochemical properties of CF. This is the first report on the ionic and biochemical composition of the CF of sterlet, Siberian and Russian sturgeon, which can be used as a reference point for further development of artificial media for the short‐term storage of unfertilized sturgeon eggs as well as for the standardization of the fertilization protocol for these species in controlled reproduction.  相似文献   

17.
The swimming capacity of Barbus bocagei was measured with the critical swimming speed (Ucrit) standard test in a modified Bla?ka‐type swim tunnel. Sixty B. bocagei were tested and they exhibited a mean ±s .d . Ucrit of 0·81 ± 0·11 m s?1 or 3·1 ± 0·86 total lengths per second (LT s?1). Sex had no effect on Ucrit but significant differences were found between the swimming performance of fish with distinct sizes.  相似文献   

18.
The effects of gender, maturity, intersex condition and annual and seasonal variability were studied on hematology and plasma chemistry of wild shortnose sturgeon Acipenser brevirostrum from the Delaware River. A total of 68 fish were captured by gill net and examined in May‐June, and 61 additional fish were captured and examined in November during 2006–2011. Total leukocyte counts (WBC), leukogram, PCV and the plasmatic concentrations of 13 biochemical analytes were measured from these fish using standard clinical methods. Season and gender had no effect on hematologic indices, but PCV was inversely related to maturity of fish (robust intervals: 27–40% in immature fish, 21–36% in mature fish). Significant annual variation was detected in eosinophil (annual range in robust interval: 0–1176 lower limit, 670–7882 upper limit) and monocyte (0–210 lower limit, 560–1980 upper limit) counts (cells μl?1). The lower limit of the robust interval varied annually by as much as 6% for sodium and 12% for chloride, while the upper limit of the robust interval varied annually by as much as 8% for sodium and 15% for chloride. Seasonal differences in mean sodium and chloride (6–7% higher in autumn) and proteins (5–13% higher in autumn) may reflect environmentally induced changes in osmoregulation, while aspartate aminotransferase was 38–55% higher in the spring. In females, calcium and total protein were highest in mature fish (robust intervals: 10.5–22.1 mg dl?1 Ca2+, 4.1–6.9 g dl?1 TP) compared to immature (7.3–16.9 mg dl?1 Ca2+, 2.8–5.2 g dl?1 TP) and developing fish (7.8–18.9 mg dl?1 Ca2+, 3.2–5.6 g dl?1 TP), indicating changes associated with vitellogenesis. Glucose was significantly higher in females (robust interval: 23–167 mg dl?1) than males (35–138 mg dl?1), possibly indicating gender‐based differences in energetic requirements. Intersex condition was associated with lowered glucose, potassium and creatinine phosphokinase. Reference intervals reported here are useful for evaluating the health and physiological condition of shortnose sturgeon.  相似文献   

19.
The overall goal of this study was to characterize the age, growth, condition, and total catch of lake sturgeon Acipenser fulvescens in a remnant population associated with a Lake Michigan, USA tributary. Lake sturgeon were captured (2008–2013) using large‐mesh (25.4 and 30.5 cm) and small‐mesh (6.4 and 7.6 cm) gill netting in Muskegon Lake (which connects the Muskegon River to Lake Michigan), and adults were captured with boat electrofishing in the Muskegon River. A total of 268 unique lake sturgeon (24.8–191.0 cm total length; <0.1–59.5 kg weight) were captured. Of these, 180 fish were aged using pectoral fin rays, representing 27 age cohorts and a mean age of 7.6 years. The weight–length relationship for lake sturgeon was log10 (W) = 3.446·log10 (L) ? 6.163 and the von Bertalanffy growth model was = 180.719 [1?e?0.093(+ 0.902)], where W was wet weight (kg), L was total length (cm), and t was age (years). Mean growth rate of juveniles (ages 3–6) recaptured in successive years was 8.6 cm/year and 558 g/year. Annual catch of adults during the spawning migration suggested that the number of spawners each year was low (i.e., probably <50 individuals in most years). Natural reproduction appeared to be occurring given the catch of juvenile lake sturgeon in Muskegon Lake. However, recovery of this remnant population is uncertain given the population age structure and low rate of adult recruitment during the study.  相似文献   

20.
The direct and indirect effects of a simulated, calcarenite‐based dredge material on eggs and larvae of pink snapper Pagrus auratus were assessed. Direct effects were assessed by measuring hatch rate or survival of eggs and pre‐feeding larvae, respectively, over a range of concentrations and exposure durations. Exposure of eggs to suspended solid concentrations up to 10 000 mg l?1 for 24 h did not affect egg buoyancy or hatch rate, despite sediment adherence occurring at the two highest concentrations tested. Newly hatched larvae, whose mouths were still closed, were relatively tolerant of suspended solids, with a 12 h lethal concentration resulting in 50% mortality, LC50, of 2020 mg l?1 and a first observable effect concentration of 150 mg l?1. Once the larvae's mouths opened, tolerance was significantly reduced, with a 12 h LC50 of 157 mg l?1 and a first observable effect concentration of 4 mg l?1. Tolerance of larvae to suspended solids was negatively correlated with suspended solids concentration and exposure time, with exposure durations of ≤6 h being significantly less detrimental than those of 9 h or more. Indirect effects to larvae were assessed by measuring ingestion of copepod nauplii by 10 and 15 days post‐hatch (dph) larvae at sediment concentrations from 0 to 200 mg l?1 in 50 mg l?1 increments over 4 h. Ingestion was not significantly affected by sediment for 10 dph larvae, but by 15 dph, sediment had a far greater impact on ingestion, with larvae in all sediment treatments eating significantly fewer copepods than those in the control.  相似文献   

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