Folding and Self-Assembly of the TatA Translocation Pore Based on a Charge Zipper Mechanism

1. Download : Download high-res image (202KB)
2. Download : Download full-size image

Highlights? Charge zippers as a new concept for folding and assembly of membrane proteins ? 3D structure of TatA pore explains translocation of folded proteins across membrane ? Ladders of salt bridges connect an amphiphilic palisade that can span the bilayer ? MD simulations and specific charge mutations support the charge zipper model     

Multiscale Simulations Reveal Conserved Patterns of Lipid Interactions with Aquaporins

1. Download : Download high-res image (329KB)
2. Download : Download full-size image

Highlights? Multiscale MD simulations predict the interactions of lipids with membrane proteins ? The method is evaluated via comparison with the structure of Aqp0 in a membrane ? Simulations of aquaporins reveal a broadly conserved protein/lipid interface ? The results suggest interchange between annular and bulk lipids     

Plasma Membrane Tethering of the Cortical ER Necessitates Its Finely Reticulated Architecture

1. Download : Download high-res image (520KB)
2. Download : Download full-size image

Highlights? The VAP proteins sustain the ER-plasma membrane attachment in fission yeast ? Footprint of the ER on the cortex is functionally insulated from the cytosol ? Division-site placement requires a patchwork of cortical sites accessible to cytosol     

The Centrosome Regulates the Rab11- Dependent Recycling Endosome Pathway at Appendages of the Mother Centriole

1. Download : Download high-res image (153KB)
2. Download : Download full-size image

Highlights? Endosome-proteins localize to centrosomes independent of membranes ? Rab11 and its modulators localize to mother centriole appendages ? Mother centriole appendages regulate membrane recycling through Rab11     

Dynamin 2 Potentiates Invasive Migration of Pancreatic Tumor Cells through Stabilization of the Rac1 GEF Vav1

1. Download : Download high-res image (180KB)
2. Download : Download full-size image

Highlights? Dyn2 promotes lamellipodia formation and pancreatic tumor cell migration ? Direct binding between Dyn2 and Vav1 promotes Rac1 activation and migration ? Dyn2 binding protects Vav1 from degradation by the lysosome ? Vav1 is targeted for degradation through an interaction with Hsc70     

Out but Not In: The Large Transmembrane β-Barrel Protein FhuA Unfolds but Cannot Refold via β-Hairpins

1. Download : Download high-res image (119KB)
2. Download : Download full-size image

Highlights? Mechanical un- and refolding studies of single large transmembrane β-barrel proteins ? FhuA unfolds via highly reproducible steps formed by single β-hairpins ? Once unfolded, FhuA folds into highly irreproducible structures ? To assist folding into the membrane, FhuA may require cofactors     

Keeping cell identity in Arabidopsis requires PRC1 RING-finger homologs that catalyze H2A monoubiquitination

1. Download : Download high-res image (139KB)
2. Download : Download full-size image

Highlights? AtBMI1A/B proteins prevent misexpression of embryonic traits in somatic cells ? PRC2 and AtBMI1A/B proteins maintain cells in their differentiated state ? AtBMI1A/B proteins mediate H2A monoubiquitination     

Triacylglycerol Synthesis Enzymes Mediate Lipid Droplet Growth by Relocalizing from the ER to Lipid Droplets

1. Download : Download high-res image (442KB)
2. Download : Download full-size image

Highlights? Triacylglyceride (TG) synthesis is coupled with lipid droplet (LD) growth ? Two LD populations exist: growing LDs, containing TG enzymes, and small LDs ? Specific TG synthesis enzymes move from the ER to LDs through membrane bridges ? LD localization of TG enzymes mediates expansion of a subset of LDs     

Zona Pellucida Domain Proteins Remodel the Apical Compartment for Localized Cell Shape Changes

1. Download : Download high-res image (334KB)
2. Download : Download full-size image

Highlights? In Drosophila epidermis, shavenbaby controls ZPD gene expression ? Apical cell shape remodeling relies on the coordinated action of ZPD genes ? ZPD proteins define sub-regions in the apical extracellular matrix (aECM) ? ZPD products maintain localized apical membrane/aECM interactions.     

The Myosin Chaperone UNC-45 Is Organized in Tandem Modules to Support Myofilament Formation in C. elegans

1. Download : Download high-res image (299KB)
2. Download : Download full-size image

Highlights? UNC-45 self-assembles a docking platform for multiple chaperone and client proteins ? Hsp70/90 and myosin bind to specific sites on the TPR and UCS domains of UNC-45 ? The UNC-45 multimer offers the proper spacing to couple myosin folding and assembly ? In vivo, UNC-45 chains support the formation of fully functional sarcomeric repeats     

Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligase

1. Download : Download high-res image (188KB)
2. Download : Download full-size image

Highlights? The crystal structure of the heterotetrameric CRL3-SPOP ubiquitin ligase is reported ? Substrate adaptor proteins are recruited to CRL complexes through a signature motif ? Tandem BTB and BACK domains potentiate assembly and activity of the CRL3-SPOP ligase ? The SPOPL negative regulator creates a molecular rheostat to fine-tune CRL3 activity     

Two distinct binding modes define the interaction of Brox with the C-terminal tails of CHMP5 and CHMP4B

1. Download : Download high-res image (435KB)
2. Download : Download full-size image

Highlights? CHMP5 binds and recruits Brox to membrane fractions through its C-terminal tail ? The CHMP5 C-terminal tail adopts a tandem β-hairpin structure ? The CHMP4B C-terminal tail α helix binds at a concave surface of Brox ? The CHMP5 β-hairpins and CHMP4B α helix bind the same surface at Brox     

The Mycobacterium tuberculosis very-long-chain fatty acyl-CoA synthetase: structural basis for housing lipid substrates longer than the enzyme

1. Download : Download high-res image (131KB)
2. Download : Download full-size image

Highlights? M. tuberculosis FadD13 is a peripheral membrane protein ? Mutagenesis reveals an arginine-rich patch as the site for membrane interaction ? A hydrophobic tunnel extends from the active site to the positively charged patch ? The architecture lets substrates reside partly in the membrane during catalysis     

A Network Model of the Molecular Organization of Chromatin in Drosophila

1. Download : Download high-res image (434KB)
2. Download : Download full-size image

Highlights? DamID identifies 42 previously unknown chromatin proteins ? Binding maps of new and known chromatin components yield a chromatin network model ? The network model predicts functions of most of the 42 new chromatin proteins ? The chromatin protein network is functionally compartmentalized     

Widespread Regulation of Translation by Elongation Pausing in Heat Shock

1. Download : Download high-res image (227KB)
2. Download : Download full-size image

Highlights? Global translation elongation pause at 5′ end of ORFs in severe heat shock ? Modulation of Hsp70 levels/activity affects elongation pausing ? Pausing is associated with hydrophobic N termini ? Hsp70 shows reduced ribosome association and altered interactions in heat stress     

Sculpting of DNA at Abasic Sites by DNA Glycosylase Homolog Mag2

1. Download : Download high-res image (141KB)
2. Download : Download full-size image

Highlights? Unflipped AP site stabilized by helix-hairpin-helix DNA glycosylase homolog Mag2 ? Nonenzymatic AP site recognition and DNA sculpting ? Sculpting of DNA conformation enhances substrate transfer between proteins     

Cell-free Expressed Bacteriorhodopsin in Different Soluble Membrane Mimetics: Biophysical Properties and NMR Accessibility

1. Download : Download high-res image (185KB)
2. Download : Download full-size image

Highlights? Bacteriorhodopsin (bR) is NMR accessible in nanodiscs and amphipols ? Nonconventional surfactants offer increased stability for cell-free expressed bR ? The bR-membrane interface is highly adaptable in the selected membrane mimetics ? Applications and limitations of the environments for NMR studies are discussed     

Protein Determinants of Meiotic DNA Break Hot Spots

1. Download : Download high-res image (176KB)
2. Download : Download full-size image

Highlights? DSB hot spots for meiotic recombination are specified by largely unknown mechanisms ? Linear element proteins, required for most DSBs, are highly enriched at hot spots ? These proteins, the first known global hot spot determinants, bind without DSB formation ? Hot spot determination by these proteins provides a paradigm for crossover control     

Selective Chemical Crosslinking Reveals a Cep57-Cep63-Cep152 Centrosomal Complex

1. Download : Download high-res image (183KB)
2. Download : Download full-size image

Highlights? Selective chemical crosslinking identifies novel protein-protein interactions ? Superresolution microcopy defines the localization of centrosomal proteins ? Cep57, Cep63, and Cep152 form a ring-like centrosomal complex     

Mycobacterium tuberculosis activates the DNA-dependent cytosolic surveillance pathway within macrophages

1. Download : Download high-res image (154KB)
2. Download : Download full-size image

Highlights? M. tuberculosis permeabilizes phagosomes and activates cytosolic signaling pathways ? Host cytoplasmic DNA receptors sense M. tuberculosis extracellular DNA ? DNA recognition activates the STING/TBK1/IRF3 pathway ? Cytosolic sensing promotes M. tuberculosis infection