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1.
High-density genetic map of durum wheat × wild emmer wheat based on SSR and DArT markers 总被引:1,自引:1,他引:0
Peleg Z Saranga Y Suprunova T Ronin Y Röder MS Kilian A Korol AB Fahima T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(1):103-115
A genetic linkage map of tetraploid wheat was constructed based on a cross between durum wheat [Triticum turgidum ssp. durum (Desf.) MacKey] cultivar Langdon and wild emmer wheat [T. turgidum ssp. dicoccoides (K?rn.) Thell.] accession G18-16. One hundred and fifty-two single-seed descent derived F(6) recombinant inbred lines (RILs) were analyzed with a total of 690 loci, including 197 microsatellite and 493 DArT markers. Linkage analysis defined 14 linkage groups. Most markers were mapped to the B-genome (60%), with an average of 57 markers per chromosome and the remaining 40% mapped to the A-genome, with an average of 39 markers per chromosome. To construct a stabilized (skeleton) map, markers interfering with map stability were removed. The skeleton map consisted of 307 markers with a total length of 2,317 cM and average distance of 7.5 cM between adjacent markers. The length of individual chromosomes ranged between 112 cM for chromosome 4B to 217 cM for chromosome 3B. A fraction (30.1%) of the markers deviated significantly from the expected Mendelian ratios; clusters of loci showing distorted segregation were found on chromosomes 1A, 1BL, 2BS, 3B, and 4B. DArT markers showed high proportion of clustering, which may be indicative of gene-rich regions. Three hundred and fifty-two new DArT markers were mapped for the first time on the current map. This map provides a useful groundwork for further genetic analyses of important quantitative traits, positional cloning, and marker-assisted selection, as well as for genome comparative genomics and genome organization studies in wheat and other cereals. 相似文献
2.
Blanco A Simeone R Cenci A Gadaleta A Tanzarella OA Porceddu E Salvi S Tuberosa R Figliuolo G Spagnoletti P Röder MS Korzun V 《Cellular & molecular biology letters》2004,9(3):529-541
A set of recombinant inbred lines (RIL) derived from a cross between the cultivar Messapia of durum wheat (Triticum turgidum var. durum) and the accession MG4343 of T. turgidum var. dicoccoides was analysed to increase the number of assigned markers and the resolution of the previously constructed genetic linkage map. An updated map of the durum wheat genome consisting of 458 loci was constructed. These loci include 261 Restriction Fragment Length Polymorphisms (RFLPs), 91 microsatellites (Simple Sequence Repeats, SSRs), 87 Amplified Fragment Length Polymorphisms (AFLPs), two ribosomal genes, and nine biochemical (seven seed storage proteins and two isozymes) and eight morphological markers. The loci were mapped on all 14 chromosomes of the A and B genomes, and covered a total distance of 3038.4 cM with an average distance of 6.7 cM between adjacent markers. The molecular markers were evenly distributed between the A and the B genomes (240 and 218 markers, respectively). An additional forty loci (8.8%) could not be assigned to a specific linkage group. A fraction (16.4%) of the markers significantly deviated from the expected Mendelian ratios; clusters of loci showing distorted segregation were found on the 1B, 2A, 2B, 3A, 4A, 7A and 7B chromosomes. The genetic lengths of the chromosomes range from 148.8 cM (chromosome 6B) to 318.0 cM (chromosome 2B) and approximately concur with their physical lengths. Chromosome 2B has the largest number of markers (47), while the chromosomes with the fewest markers are 3A and 6B (23). There are two gaps larger than 40 cM on chromosomes 2A and 3B. The durum wheat map was compared with the published maps of bread and durum wheats; the order of most common RFLP and SSR markers on the 14 chromosomes of the A and B genomes were nearly identical. A core-map can be extracted from the high-density Messapia x dicoccoides map and a subset of uniformly distributed markers can be used to detect and map quantitative trait loci. 相似文献
3.
Integration of dinucleotide microsatellites from hexaploid bread wheat into a genetic linkage map of durum wheat 总被引:13,自引:0,他引:13
V. Korzun M. S. Röder K. Wendehake A. Pasqualone C. Lotti M. W. Ganal A. Blanco 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(8):1202-1207
Seventy nine microsatellite markers from hexaploid bread wheat (T. aestivum L.) were integrated into a genetic linkage map of durum wheat (T. turgidum ssp. durum (Desf.) Huns.) created by RFLP segregation data from a population of 65 recombinant inbred lines. The results indicate a
relatively even distribution of microsatellite loci and demonstrate that microsatellite markers from hexaploid wheat provide
an excellent source of molecular markers for use in the genetics and breeding of durum wheat.
Received: 16 July 1998 / Accepted: 13 October 1998 相似文献
4.
A high‐density,SNP‐based consensus map of tetraploid wheat as a bridge to integrate durum and bread wheat genomics and breeding 下载免费PDF全文
Marco Maccaferri Andrea Ricci Silvio Salvi Sara Giulia Milner Enrico Noli Pier Luigi Martelli Rita Casadio Eduard Akhunov Simone Scalabrin Vera Vendramin Karim Ammar Antonio Blanco Francesca Desiderio Assaf Distelfeld Jorge Dubcovsky Tzion Fahima Justin Faris Abraham Korol Andrea Massi Anna Maria Mastrangelo Michele Morgante Curtis Pozniak Amidou N'Diaye Steven Xu Roberto Tuberosa 《Plant biotechnology journal》2015,13(5):648-663
5.
C. Du G. E. Hart 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(5):645-653
Triticum turgidum L. var ‘durum’ cv ‘Langdon’-T. t. var ‘dicoccoides’ chromosome 6A and 6B recombinant substitution lines (RSLs) and a F2 population derived from a ‘Langdon’-T. t. var ‘dicoccoides’ disomic chromosome 6A substitution lineבLangdon’ cross were analyzed with the objective of markedly increasing
the number of markers assigned to and the resolution of previously constructed 6A and 6B linkage maps. Fifty-seven markers
were added to the 6A RSL-population map, which now consists of 73 markers that span 111 cM, and 40 markers were added to the
6B RSL-population map, which now consists of 56 markers that span 123 cM. With the exception of 2 6B loci, all of the loci
on the two RSL-population maps were ordered at a LOD score ≥3.0. Thirty-seven orthologous markers were mapped in the two chromosomes
and colinearity between them is strongly indicated. The 6A RSL-population map and the F2-population map are highly similar, indicating that the former population, which consists of 66 lines, can be reliably used
for mapping, as was previously demonstrated for the 6B RSL population. In the absence of selection and genetic drift, the
lines in a RSL population, except at loci in the substituted/recombined chromosome, should be near-isogenic. An unexpected
finding was that at least 26 and possibly 29 of the RFLPs detected in the RSL populations (18% of the markers analyzed) are
not located in the substituted/recombined chromosomes. Linkage analysis of the markers disclosed that at least 19 of them
are located in six or seven segments that span approximately 10 cM and 17 cM of the genetic lengths of 6B and 6A, respectively,
in the 6A and 6B RSL populations, respectively, a finding that suggests that 40 or more alien segments spanning 8–15% of the
genetic length of the 13 unsubstituted chromosomes are present in both of the RSL populations. Alien alleles are fixed in
many RSLs for most of the markers, in most cases at a frequency consistent with theoretical expectations. Highly distorted
segregation favoring the alien allele was detected for all of the markers in 2 of the segments, however. Nine of the markers
were among those mapped in the substituted/recombined chromosomes; the linkage data obtained for the other 10 was sufficient
to assign them to approximate map positions.
Received: 12 June 1997 / Accepted: 6 October 1997 相似文献
6.
Molecular genetic maps of the group 6 chromosomes of hexaploid wheat (Triticum aestivum L. em. Thell.). 总被引:3,自引:0,他引:3
C L Marino N A Tuleen G E Hart J C Nelson M E Sorrells Y H Lu P Leroy C R Lopes 《Génome》1996,39(2):359-366
Restriction fragment length polymorphism (RFLP) maps of chromosomes 6A, 6B, and 6D of hexaploid wheat (Triticum aestivum L. em. Thell.) have been produced. They were constructed using a population of F7-8 recombinant inbred lines derived from a synthetic wheat x bread wheat cross. The maps consist of 74 markers assigned to map positions at a LOD >= 3 (29 markers assigned to 6A, 24 to 6B, and 21 to 6D) and 2 markers assigned to 6D ordered at a LOD of 2.7. Another 78 markers were assigned to intervals on the maps. The maps of 6A, 6B, and 6D span 178, 132, and 206 cM, respectively. Twenty-one clones detected orthologous loci in two homoeologues and 3 detected an orthologous locus in each chromosome. Orthologous loci are located at intervals of from 1.5 to 26 cM throughout 70% of the length of the linkage maps. Within this portion of the maps, colinearity (homosequentiality) among the three homoeologues is strongly indicated. The remainder of the linkage maps consists of three segments ranging in length from 47 to 60 cM. Colinearity among these chromosomes and other Triticeae homoeologous group 6 chromosomes is indicated and a consensus RFLP map derived from maps of the homoeologous group 6 chromosomes of hexaploid wheat, tetraploid wheat, Triticum tauschii, and barley is presented. Key words : RFLP, wheat, linkage maps, molecular markers. 相似文献
7.
A high-density consensus map of A and B wheat genomes 总被引:1,自引:0,他引:1
Daniela Marone Giovanni Laidò Agata Gadaleta Pasqualina Colasuonno Donatella B. M. Ficco Angelica Giancaspro Stefania Giove Giosué Panio Maria A. Russo Pasquale De Vita Luigi Cattivelli Roberto Papa Antonio Blanco Anna M. Mastrangelo 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,125(8):1619-1638
A durum wheat consensus linkage map was developed by combining segregation data from six mapping populations. All of the crosses were derived from durum wheat cultivars, except for one accession of T. ssp. dicoccoides. The consensus map was composed of 1,898 loci arranged into 27 linkage groups covering all 14 chromosomes. The length of the integrated map and the average marker distance were 3,058.6 and 1.6?cM, respectively. The order of the loci was generally in agreement with respect to the individual maps and with previously published maps. When the consensus map was aligned to the deletion bin map, 493 markers were assigned to specific bins. Segregation distortion was found across many durum wheat chromosomes, with a higher frequency for the B genome. This high-density consensus map allowed the scanning of the genome for chromosomal rearrangements occurring during the wheat evolution. Translocations and inversions that were already known in literature were confirmed, and new putative rearrangements are proposed. The consensus map herein described provides a more complete coverage of the durum wheat genome compared with previously developed maps. It also represents a step forward in durum wheat genomics and an essential tool for further research and studies on evolution of the wheat genome. 相似文献
8.
Genetic and physical mapping of new EST-derived SSRs on the A and B genome chromosomes of wheat 总被引:2,自引:0,他引:2
A. Gadaleta A. Giancaspro S. L. Giove S. Zacheo G. Mangini R. Simeone A. Signorile A. Blanco 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(5):1015-1025
The availability of genetic maps and phenotypic data of segregating populations allows to localize and map agronomically important
genes, and to identify closely associated molecular markers to be used in marker-assisted selection and positional cloning.
The objective of the present work was to develop a durum wheat intervarietal genetic and physical map based on genomic microsatellite
or genomic simple sequence repeats (gSSR) markers and expressed sequence tag (EST)-derived microsatellite (EST-SSR) markers.
A set of 122 new EST-SSR loci amplified by 100 primer pairs was genetically mapped on the wheat A and B genome chromosomes.
The whole map also comprises 149 gSSR markers amplified by 120 primer pairs used as anchor chromosome loci, two morphological
markers (Black colour, Bla1, and spike glaucousness, Ws) and two seed storage protein loci (Gli-A2 and Gli-B2). The majority of SSR markers tested (182) was chromosome-specific. Out of 275 loci 241 loci assembled in 25 linkage groups
assigned to the chromosomes of the A and B genome and 34 remained unlinked. A higher percentage of markers (54.4%), localized
on the B genome chromosomes, in comparison to 45.6% distributed on the A genome. The whole map covered 1,605 cM. The B genome
accounted for 852.2 cM of genetic distance; the A genome basic map spanned 753.1 cM with a minimum length of 46.6 cM for chromosome
5A and a maximum of 156.2 cM for chromosome 3A and an average value of 114.5 cM. The primer sets that amplified two or more
loci mapped to homoeologous as well as to non-homoeologous sites. Out of 241 genetically mapped loci 213 (88.4%) were physically
mapped by using the nulli-tetrasomic, ditelosomic and a stock of 58 deletion lines dividing the A and B genome chromosomes
in 94 bins. No discrepancies concerning marker order were observed but the cytogenetic maps revealed in some cases small genetic
distance covered large physical regions. Putative function for mapped SSRs were assigned by searching against GenBank nonredundant
database using TBLASTX algorithms. 相似文献
9.
I. Eujayl M. Baum W. Powell W. Erskine E. Pehu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):83-89
A genetic linkage map of Lens sp. was constructed with 177 markers (89 RAPD, 79 AFLP, six RFLP and three morphological markers) using 86 recombinant inbred
lines (F6:8) obtained from a partially interspecific cross. The map covered 1073 cM of the lentil genome with an average distance of
6.0 cM between adjacent markers. Previously mapped RFLP markers were used as anchor probes. The morphological markers, pod
indehiscence, seed-coat pattern and flower-color loci were mapped. Out of the total linked loci, 8.4% showed segregation distortion.
More than one-fourth of the distorted loci were clustered in one linkage group. AFLP markers showed more segregation distortion
than the RAPD markers. The AFLP and RAPD markers were intermingled and clustering of AFLPs was seldom observed. This is the
most extensive genetic linkage map of lentil to-date. The marker density of this map could be used for the identification
of markers linked to quantitative trait loci in this population.
Received: 6 November 1997 / Accepted: 10 February 1998 相似文献
10.
Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat 总被引:2,自引:0,他引:2
Xunfen Chen Justin D. Faris Jinguo Hu Robert W. Stack Tika Adhikari Elias M. Elias Shahryar F. Kianian Xiwen Cai 《Molecular breeding : new strategies in plant improvement》2007,19(2):113-124
Fusarium head blight (FHB) is a devastating disease of cultivated wheat worldwide. Partial resistance to FHB has been identified
in common wheat (Triticum aestivum L.). However, sources of effective FHB resistance have not been found in durum wheat (T. turgidum L. var. durum). A major FHB resistance quantitative trait loci (QTL), Qfhs.ndsu-3AS, was identified on chromosome 3A of T.
dicoccoides, a wild relative of durum wheat. Here, we saturated the genomic region containing the QTL using EST-derived target region
amplified polymorphism (TRAP), sequence tagged site (STS), and simple sequence repeat (SSR) markers. A total of 45 new molecular
marker loci were detected on chromosome 3A and the resulting linkage map consisted of 55 markers spanning a genetic distance
of 277.2 cM. Qfhs.ndsu-3AS was positioned within a chromosomal interval of 11.5 cM and is flanked by the TRAP marker loci, Xfcp401 and Xfcp397.2. The average map distance between the marker loci within this QTL region was reduced from 4.9 cM in the previous study to
3.5 cM in the present study. Comparative mapping indicated that Qfhs.ndsu-3AS is not homoeologous to Qfhs.ndsu-3BS, a major FHB QTL derived from the common wheat cultivar Sumai 3. These results facilitate our efforts toward map-based cloning
of Qfhs.ndsu-3AS and utilization of this QTL in durum wheat breeding via marker-assisted selection. 相似文献
11.
An integrated DArT-SSR linkage map of durum wheat 总被引:2,自引:0,他引:2
Paola Mantovani Marco Maccaferri Maria Corinna Sanguineti Roberto Tuberosa Ilaria Catizone Peter Wenzl Brent Thomson Jason Carling Eric Huttner Enzo DeAmbrogio Andrzej Kilian 《Molecular breeding : new strategies in plant improvement》2008,22(4):629-648
Genetic mapping in durum wheat (Triticum durum Desf.) is constrained by its large genome and allopolyploid nature. We developed a Diversity Arrays Technology (DArT) platform
for durum wheat to enable efficient and cost-effective mapping and molecular breeding applications. Genomic representations
from 56 durum accessions were used to assemble a DArT genotyping microarray. Microsatellite (SSR) and DArT markers were mapped
on a durum wheat recombinant inbred population (176 lines). The integrated DArT-SSR map included 554 loci (162 SSRs and 392
DArT markers) and spanned 2022 cM (5 cM/marker on average). The DArT markers from durum wheat were positioned in respect to
anchor SSRs and hexaploid wheat DArT markers. DArT markers compared favourably to SSRs to evaluate genetic relationships among
the durum panel, with 1315 DArT polymorphisms found across the accessions. Combining DArT and SSR platforms provides an efficient
and rapid method of generating linkage maps in durum wheat. 相似文献
12.
M. M. Nachit I. Elouafi A. Pagnotta A. El Saleh E. Iacono M. Labhilili A. Asbati M. Azrak H. Hazzam D. Benscher M. Khairallah J.-M. Ribaut O. A. Tanzarella E. Porceddu M. E. Sorrells 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(2-3):177-186
Durum wheat (Triticum turgidum L. var. durum) is an economically and nutritionally important cereal crop in the Mediterranean region. To further our understanding
of durum genome organization we constructed a durum linkage map using restriction fragment length polymorphisms (RFLPs), simple
sequence repeats (SSRs) known as Gatersleben wheat microsatellites (GWMs), amplified fragment length polymorphisms (AFLPs),
and seed storage proteins (SSPs: gliadins and glutenins). A population of 110 F9 recombinant inbred lines (RILs) was derived from an intraspecific cross between two durum cultivars, Jennah Khetifa and Cham
1. The two parents exhibit contrasting traits for resistance to biotic and abiotic stresses and for grain quality. In total,
306 markers have been placed on the linkage map – 138 RFLPs, 26 SSRs, 134 AFLPs, five SSPs, and three known genes (one pyruvate
decarboxylase and two lipoxygenases). The map is 3598 cM long, with an average distance between markers of 11.8 cM, and 12.1%
of the markers deviated significantly from the expected Mendelian ratio 1:1. The molecular markers were evenly distributed
between the A and B genomes. The chromosome with the most markers is 1B (41 markers), followed by 3B and 7B, with 25 markers
each. The chromosomes with the fewest markers are 2A (11 markers), 5A (12 markers), and 4B (15 markers). In general, there
is a good agreement between the map obtained and the Triticeae linkage consensus maps. This intraspecific map provides a useful tool for marker-assisted selection and map-based breeding
for resistance to biotic and abiotic stresses and for improvement of grain quality.
Received: 14 February 2000 / Accepted: 28 April 2000 相似文献
13.
Sishen Li Jizeng Jia Xianyun Wei Xiaocun Zhang Linzhi Li Haimei Chen Yuding Fan Haiyan Sun Xinhua Zhao Tiandong Lei Yunfong Xu Fangshan Jiang Honggang Wang Lihui Li 《Molecular breeding : new strategies in plant improvement》2007,20(2):167-178
A new genetic linkage map was constructed based on recombinant inbred lines (RILs) derived from the cross between the Chinese
winter wheat (Triticum aestivum L.) varieties, Chuang 35050 and Shannong 483 (ChSh). The map included 381 loci on all the wheat chromosomes, which were composed
of 167 SSR, 94 EST-SSR, 76 ISSR, 26 SRAP, 15 TRAP, and 3 Glu loci. This map covered 3636.7 cM with 1327.7 cM (36.5%), 1485.5 cM (40.9%), and 823.5 cM (22.6%) for A, B, and D genome,
respectively, and contained 13 linkage gaps. Using the RILs and the map, we detected 46 putative QTLs on 12 chromosomes for
grain yield (GY) per m2, thousand-kernel weight (TKW), spike number (SN) per m2, kernel number per spike (KNS), sterile spikelet number per spike (SSS), fertile spikelet number per spike (FSS), and total
spikelet number per spike (TSS) in four environments. Each QTL explained 4.42–70.25% phenotypic variation. Four QTL cluster
regions were detected on chromosomes 1D, 2A, 6B, and 7D. The most important QTL cluster was located on chromosome 7D near
the markers of Xwmc31, Xgdm67, and Xgwm428, in which 8 QTLs for TKW, SN, SSS and FSS were observed with very high contributions (27.53–67.63%). 相似文献
14.
Development of a chromosomal arm map for wheat based on RFLP markers 总被引:16,自引:0,他引:16
J. A. Anderson Y. Ogihara M. E. Sorrells S. D. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,83(8):1035-1043
Summary A chromosomal arm map has been developed for common wheat (Triticum aestivum L. em. Thell.) using aneuploid stocks to locate more than 800 restriction fragments corresponding to 210 low-copy DNA clones from barley cDNA, oat cDNA, and wheat genomic libraries. The number of restriction fragments per chromosome arm correlates moderately well with relative DNA content and length of somatic chromosomes. The chromosomal arm locations of loci detected with 6 different clones support an earlier hypothesis for the occurrence of a two-step translocation (4AL to 5AL, 5AL to 7BS, and 7BS to 4AL) in the ancestral wheat genomes. In addition, 1 clone revealed the presence of a 5AL segment translocated to 4AL. Anomalies in aneuploid stocks were also observed and can be explained by intrahomoeologous recombination and polymorphisms among the stocks. We view the development of this chromosomal arm map as a complement to, rather than as a substitute for, a conventional RFLP linkage map in wheat.Paper No. 802 of the Cornell Plant Breeding Series 相似文献
15.
Molecular mapping of wheat. Homoeologous group 3. 总被引:21,自引:0,他引:21
J C Nelson A E Deynze M E Sorrells E Autrique Y H Lu S Negre M Bernard P Leroy 《Génome》1995,38(3):525-533
A prerequisite for molecular level genetic studies and breeding in wheat is a molecular marker map detailing its similarities with those of other grass species in the Gramineae family. We have constructed restriction fragment length polymorphism maps of the A-, B-, and D-genome chromosomes of homoeologous group 3 of hexaploid wheat (Triticum aestivum L. em. Thell) using 114 F7-8 lines from a synthetic x bread wheat cross. The map consists of 58 markers spanning 230 cM on chromosome 3A, 62 markers spanning 260 cM on 3B, and 40 markers spanning 171 cM on 3D. Thirteen libraries of genomic or cDNA clones from wheat, barley, and T. tauschii, the wheat D genome donor, are represented, facilitating the alignment and comparison of these maps with maps of other grass species. Twenty-four clones reveal homoeoloci on two of the three genomes and the associated linkages are largely comparable across genomes. A consensus sequence of orthologous loci in grass species genomes is assembled from this map and from existing maps of the chromosome-3 homoeologs in barley (Hordeum spp.), T. tauschii, and rice (Oryza spp.). It illustrates the close homoeology among the four species and the partial homoeology of wheat chromosome 3 with oat (Avena spp.) chromosome C. Two orthologous red grain color genes, R3 and R1, are mapped on chromosome arms 3BL and 3DL. 相似文献
16.
L. Gentzbittel E. Mestries S. Mouzeyar F. Mazeyrat S. Badaoui F. Vear D. Tourvieille de Labrouhe P. Nicolas 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):218-234
A map of the sunflower genome, based on expressed sequences and consisting of 273 loci, was constructed. The map incorporates
data from seven F2 populations, for a total of 1115 individuals. Two hundred and fourty five loci corresponding to 170 anonymous cDNA markers
and four loci for morphological markers were mapped. We also mapped 18 loci corresponding to previously described genes or
to sequences obtained through homology cloning. The unit maps vary from 774 cM to 1060 cM, with an average value of 14 major
linkage groups. The integrated map is arranged in 17 major linkage groups including 238 loci, plus four small segments with
2–5 marker loci; and covers 1573 cM with an overall average marker interval of 7 cM. Thirty five percent of the markers were
dominant in nature and 30% showed inter-linkage group duplication without any indication of homoeologous linkage groups. Evidence
is provided for the independence of two distinct fertility restoration genes, for the presence of two loosely linked branching
loci, and for marker tightly linked to the Rf1 restoration locus. This map provides an efficient tool in breeding applications such as disease-resistance mapping, QTL analyses
and marker-assisted selection.
Received: 27 August 1998 / Accepted: 28 December 1998 相似文献
17.
L Zhang J Luo M Hao L Zhang Z Yuan Z Yan Y Liu B Zhang B Liu C Liu H Zhang Y Zheng D Liu 《BMC genetics》2012,13(1):69-8
ABSTRACT: BACKGROUND: A synthetic doubled-haploid hexaploid wheat population, SynDH1, derived from the spontaneous chromosome doubling of triploid F1 hybrid plants obtained from the cross of hybrids Triticum turgidum ssp. durum line Langdon (LDN) and ssp. turgidum line AS313, with Aegilops tauschii ssp. tauschii accession AS60, was previously constructed. SynDH1 is a tetraploidization-hexaploid doubled haploid (DH) population because it contains recombinant A and B chromosomes from two different T. turgidum genotypes, while all the D chromosomes from Ae. tauschii are homogenous across the whole population. This paper reports the construction of a genetic map using this population. RESULTS: Of the 606 markers used to assemble the genetic map, 588 (97%) were assigned to linkage groups. These included 513 Diversity Arrays Technology (DArT) markers, 72 simple sequence repeat (SSR), one insertion site-based polymorphism (ISBP), and two high-molecular-weight glutenin subunit (HMW-GS) markers. These markers were assigned to the 14 chromosomes, covering 2048.79 cM, with a mean distance of 3.48 cM between adjacent markers. This map showed good coverage of the A and B genome chromosomes, apart from 3A, 5A, 6A, and 4B. Compared with previously reported maps, most shared markers showed highly consistent orders. This map was successfully used to identify five quantitative trait loci (QTL), including two for spikelet number on chromosomes 7A and 5B, two for spike length on 7A and 3B, and one for 1000-grain weight on 4B. However, differences in crossability QTL between the two T. turgidum parents may explain the segregation distortion regions on chromosomes 1A, 3B, and 6B. CONCLUSIONS: A genetic map of T. turgidum including 588 markers was constructed using a synthetic doubled haploid (SynDH) hexaploid wheat population. Five QTLs for three agronomic traits were identified from this population. However, more markers are needed to increase the density and resolution of this map in the future study. 相似文献
18.
M. Yaneshita S. Kaneko T. Sasakuma 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(5):751-756
A RFLP linkage map of Zoysia spp. (2n=40), a warm-season turfgrass, was constructed by using the self-pollinated progenies obtained from an interspecific
hybrid. Out of 115 DNA clones tested, 100 (87.0%), including 55 genomic clones, 38 cDNA clones, and seven gene clones encoding
photosynthetic enzymes showed allelic-RFLP banding patterns among the parental accessions. We found that 26 probes detected
two or more loci segregating in the self-pollinated progenies independently. The RFLP linkage map of Zoysia spp. consists of 115 loci in 22 linkage groups ranging in size from 12.5 cM to 141.3 cM with a total map distance of 1506 cM.
Six RFLP loci (5.4%) showed significant segregation distortion (P<0.01). Two loci out of six were mapped to linkage group II, and another two loci were mapped to group VII. In the RFLP linkage
map of zoysiagrass, five pairs of linkage groups sharing a series of duplicated loci with approximately the same order were
identified. Therefore, we conclude that Zoysia spp. with 2n=40 should be considered as allotetraploids, which might have evolved from progenitors with a basic chromosome
number of ten (x=10).
Received: 20 March 1998 / Accepted: 17 September 1998 相似文献
19.
The brittle rachis character, which causes spontaneous shattering of spikelets, has an adaptive value in wild grass species. The loci Br1 and Br2 in durum wheat (Triticum durum Desf.) and Br3 in hexaploid wheat (T. aestivum L.) determine disarticulation of rachides above the junction of the rachilla with the rachis such that a fragment of rachis is attached below each spikelet. Using microsatellite markers, the loci Br1, Br2 and Br3 were mapped on the homoeologous group 3 chromosomes. The Br2 locus was located on the short arm of chromosome 3A and linked with the centromeric marker, Xgwm32, at a distance of 13.3 cM. The Br3 locus was located on the short arm of chromosome 3B and linked with the centromeric marker, Xgwm72 (at a distance of 14.2 cM). The Br1 locus was located on the short arm of chromosome 3D. The distance of Br1 from the centromeric marker Xgdm72 was 25.3 cM. Mapping the Br1, Br2 and Br3 loci of the brittle rachis suggests the homoeologous origin of these 3 loci for brittle rachides. Since the genes for brittle rachis have been retained in the gene pool of durum wheat, the more closely linked markers with the brittle rachis locus are required to select against brittle rachis genotypes and then to avoid yield loss in improved cultivars. 相似文献
20.
QTL analysis of leaf morphology in tetraploid Gossypium (cotton) 总被引:13,自引:0,他引:13
C. Jiang R. J. Wright S. S. Woo T. A. DelMonte A. H. Paterson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(3-4):409-418
Molecular markers were used to map and characterize quantitative trait loci (QTLs) determining cotton leaf morphology and
other traits, in 180 F2 plants from an interspecific cross between a Gossypium hirsutum genotype carrying four morphological mutants, and a wild-type Gossypium barbadense. The prominent effects of a single region of chromosome 15, presumably the classical ”Okra-leaf” locus, were modified by
QTLs on several other chromosomes affecting leaf size and shape. For most traits, each parent contained some alleles with
positive effects and others with negative effects, suggesting a large potential for adapting leaf size and shape to the needs
of particular production regimes. Twenty one QTLs/loci were found for the morphological traits at LOD≥3.0 and P≤0.001, among which 14 (63.6%) mapped to D-subgenome chromosomes. Forty one more possible QTLs/loci were suggested with 2.0≤LOD<3.0
and 0.001<P≤0.01. Among all of the 62 possible QTLs (found at LOD≥2.0 and P≤0.01) for the 14 morphological traits in this study, 38 (61.3%) mapped to D-subgenome chromosomes. This reinforces the findings
of several other studies in suggesting that the D-subgenome of tetraploid cotton has been subject to a relatively greater
rate of evolution than the A-subgenome, subsequent to polyploid formation.
Received: 26 April 1999 / Accepted: 30 July 1999 相似文献