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1.
Roger H. Kennett 《In vitro cellular & developmental biology. Plant》1981,17(12):1036-1050
Summary Since the first report of hybridomas producing monoclonal antibodies by Kohler and Milstein in 1975, this technique has spread
to nearly all areas of biological, biochemical, and biomedical research. Watching the use of these methods spread from immunologists
to cell biologists, developmental biologists, biochemists and to other biological disciplines and observing the nearly logarithmic
increase in publications using these reagents has been in itself fascinating and informative. An overview of the development
of this technology and its applications is presented including the use of monoclonal antibodies to study cell surface molecules,
differentiation antigens, receptors, and histocompatibility antigens. The use of these antibodies to analyze microorganisms
and parasitic antigens as well as their use in the genetic analysis of human cell surface antigens and the detection of polymorphic
variation in enzymes and other proteins is discussed. Examples of the application of monoclonal reagents to the study of tumor
cell biology including the labeling of metastatic tumor cells and the detection of cell surface molecules implicated in the
regulation of growth control and cell division are provided.
Presented in the symposium on The Biology of Hybridomas at the 32nd Annual Meeting of the Tissue Culture Association. Washington,
D.C., June 7–11. 1981.
This symposium was supported in part by the following organizations: Bethesda Research Laboratories, Cetus Corporation, Hybritech
Incorporated, MAB-Monoclonal Antibodies, Inc., National Capital Area Branch of the Tissue Culture Association, New England
Nuclear Corporation, and Ortho Pharmaceutical Corporation. 相似文献
2.
Joseph Orly Yigal Farkash Nitzan Hershkovits Lina Mizrahi Patricia Weinberger 《In vitro cellular & developmental biology. Plant》1982,18(12):980-989
Summary The rat ovary produces an apparently low molecular weight substance that mimics the action of follitropin (FSH) on ovarian
granulosa cells in culture. Similar to FSH action, the ovarian substance (OS) induces temporal cell rounding and, later on,
intensive progestin production. However, unlike FSH, OS does not induce accumulation of cyclic AMP (cAMP) in the granulosa
cells. The ovarian factor cannot be cAMP as its action is not abolished by phosphodiesterase (PDE) treatment. Neither is it
a possible PDE inhibitor, as it does not augment cAMP accumulation in granulosa cells or Friend erythroleukemic cells induced
by FSH or PGE1, respectively. The factor is still active after heating for 20 min at 90° C but is rapidly inactivated by alkali treatment.
In addition, treatment with various proteases did not abolish the steroidogenic activity. These findings suggest a possible
novel intraovarian regulator of the granulosa cell function.
Presented in the symposium on Plant and Animal Physiology in Vitro at the 33rd Annual Meeting of the Tissue Culture Association,
San Diego, California, June 6–10, 1982.
This work was supported by the United States-Israel Binational Science Foundation, Grant 2656/81.
This symposium was supported in part by the following organizations: Bellco Glass, Inc., California Branch of the Tissue Culture
Association, Collaborative Research, Hana Media, Hybridtech, K C Biological, Inc., and Millipore Corporation. 相似文献
3.
Norman R. Klinman Kathleen A. Denis Linda A. Sherman 《In vitro cellular & developmental biology. Plant》1981,17(12):1029-1035
Summary It has long been realized that only the study of homogeneous antibodies or cell populations could enable a definitive understanding
of much of the immune mechanism. Hybridoma technology has greatly facilitated such approaches. Hybridoma antibodies have been
used to delineate both B cell and T cell subpopulations. T cell studies per se have been accomplished by the use of T cell
hybridoma cell lines producing a variety of factors. Anti-idiotypes against B cell hybridoma antibodies have been used to
characterize T cell receptors and factors. B cell studies have been facilitated by hybridomas that have made available the
immunoglobulin of pre-B cells or defective B cell lines. Hybridoma antibodies have also been used to dissect closely related
antibody families and the potential for responsiveness against a variety of antigenic determinants. Finally, hybridomas have
provided a primary source of material for protein and DNA sequence analysis. In our laboratories hybridoma antibodies derived
against the murine H-2 locus have demonstrated the ability of B cell antibodies to discriminate amongst H2 mutants—a capacity
previously attributed only to T cell specificities. Hybridoma antibodies have also been generated by fusions with antigen
stimulated neonatal B cells to provide homogeneous antibodies reflective of the earliest developmental immunoglobulin readout.
Such probes should increase our understanding of the processes involved in the generation of both the T and B cell repertoires.
Presented in the symposium on the Biology of Hybridomas at the 32nd Annual Meeting of the Tissue Culture Association. Washington,
D.C., June 7–11, 1981.
This work was supported by United States Public Health Service Grants AI 15797 and AI 15710. This symposium was supported
in part by the following organizations: Bethesda Research Laboratories, Cetus Corporation, Hybritech Incorporated, MAB-Monoclonal
Antibodies, Inc., National Capital Area Branch of the Tissue Culture Association, New England Nuclear Corporation, and Ortho
Pharmaceutical Corporation. 相似文献
4.
J. P. Mather 《In vitro cellular & developmental biology. Plant》1982,18(12):990-996
Summary Ceruloplasmin (Cp) is a copper-transport protein with ferric oxidase activity found in high concentrations in the plasma of
all vertebrates. Five cell lines (TR-1, TR-M, TR-ST, TM3, and TM4) derived from the testis can be grown in hormone-supplemented serum-free medium. Cp stimulates the growth of four of these
five cell lines in serum-free medium. The growth stimulation by Cp is not affected by the addition or deletion of free copper,
nor does copper itself elicit any significant growth response. Cp can stimulate growth also in the absence of TF suggesting
that it is not acting solely to promote Fe(III)-TF binding. A strong interaction is seen between Cp and high density lipoprotein
(HDL), with the presence of either decreasing the growth-promoting activity of the other. It is suggested that these cell
lines may provide an ideal system for studying the action of Cp at the cellular level.
Presented in the symposium on Plant and Animal Physiology in Vitro at the 33rd Annual Meeting of the Tissue Culture Association,
San Diego, California, June 6–10, 1982.
This work was supported by NIH P50 Grant HO-13541. I am grateful to Ms. Florence Kaczorowski for her expert technical assistance.
This symposium was supported in part by the following organizations: Bellco Glass, Inc., California Branch of the Tissue Culture
Association, Collaborative Research, Hana Media, Hybridtech, K C Biological, Inc., and Millipore Corporation. 相似文献
5.
John D. Minna Frank Cuttitta Steven Rosen Paul A. Bunn Jr. Desmond N. Carney Adi F. Gazdar Stephen Krasnow 《In vitro cellular & developmental biology. Plant》1981,17(12):1058-1070
Summary We have developed a screening strategy and technology to produce monoclonal antibodies with specificity for human lung cancer
cells. Mice and rats were immunized with well-characterized tissue culture lines of human small cell lung cancer (SCLC), mouse
myeloma x spleen hybrids formed by the technique of Kohler and Milstein, and the resulting culture fluids were screened for
antibody binding phenotype using a radioimmunoassay. To facilitate testing large numbers of culture fluids, a 96-well, microtiter
based, resuable, replicating device was designed. Using this, many hybridoma culture fluids were replica plated for antibody
binding tests on a series of human target cell plates. Hybrids producing antibodies that reacted with the immunizing SCLC
line and another independent SCLC line, but not with autologous B-lymphoblastoid cells derived from one of the patients, were
identified, selected, and then repeatedly recloned using the same screening strategy. With this technology, hybridomas representing
less than 0.5% of all hybrids generated could be isolated and stable antibody producing cultures derived. Such antibodies
reacted with a panel of well-characterized SCLC lines and SCLC samples taken directly from patients but not with a variety
of normal tissues. Using these antibodies we can demonstrate: tumor cell contamination of bone marrow specimens, marked heterogeneity
of antigen expression on cells within individual SCLC lines and individual patients, and inhibition of clonal growth of SCLC
lines in soft agarose assays. All of these findings have potential clinical and cell biologic application.
Presented in the symposium on The Biology of Hybridomas at the 32nd Annual Meeting of the Tissue Culture Association, Washington,
D.C., June 7–11, 1981.
This symposium was supported in part by the following organizations: Bethesda Research Laboratories, Cetus Corporation, Hybritech
Incorporated, MAB-Monoclonal Antibodies, Inc., National Capital Area Branch of the Tissue Culture Association, New England
Nuclear Corporation, and Ortho Pharmaceutical Corporation. 相似文献
6.
Tatsuhiko Ikeda Qi-fu Liu David Danielpour Jefferson B. Officer Masayoshi Iio Frances E. Leland David A. Sirbasku 《In vitro cellular & developmental biology. Plant》1982,18(12):961-979
Summary The role of polypeptide growth factors (estromedins) as mediators of estrogen-responsive mammary tumor growth is studied in
this report. Three possible new mechanisms were investigated that include endocrine, autocrine, and paracrine related growth
factors. The first hypothesis being tested is whether estrogens interact with target tissues and cause the biosynthesis and
secretion of polypeptide growth factors, which then act as mitogens for normal and neoplastic mammary tissues. Data presented
suggest that this mechanism involves estrogen interaction with uterus, kidney, and pituitary gland causing production of growth
factors, which then enter the general circulation and promote growth of distant target tissues. This is an endocrine type
mechanism. Another type of estromedin control (autocrine control) may be exerted in an autostimulatory way in which the target
tissue produces the polypeptide factors for its own growth in response to estrogen stimulation. A variation of the autocrine
mechanism may be a paracrine mechanism in which some cells of an estrogen-responsive normal or neoplastic tissue produce growth
factors that act on adjacent or neighboring cells. From the data available, all three possible types of growth factors could
be functioning synergistically to yield the final result of continuous estrogen responsive tumor growth in vivo.
Presented in the symposium on Plant and Animal Physiology in Vitro at the 33rd Annual Meeting of the Tissue Culture Association,
San Diego, California, June 6–10, 1982.
This work was supported by American Cancer Society Grant BC-255; D. A. S. is the recipient of an American Cancer Society Faculty
Research Award, FRA-212. D. D. is supported by a Rosalie B. Hite predoctoral fellowship from the Rosalie B. Hite Foundation,
Houston, Texas.
This symposium was supported in part by the following organizations: Bellco Glass, Inc., California Branch of the Tissue Culture
Association, Collaborative Research, Hana Media, Hybridtech, K C Biological, Inc., and Millipore Corporation. 相似文献
7.
Dr. Demetrios Paphadiopoulos Tazewell Wilson Robert Taber 《In vitro cellular & developmental biology. Plant》1980,16(1):49-54
Summary Lipid vesicles (liposome) have recently been shown to be a useful vehicle for the delivery of a variety of compounds to cultured
cells. Using large unilamellar vesicles composed of phosphatidylserine [LUV(PS)] we were able to encapsulate poliovirus and
purified poliovirus ribonucleic acid (RNA) and show that it can be delivered efficiently to cells in an infectious form. LUV-entrapped
poliovirus RNA produced infectious titers 100-fold higher than comparable RNA preparations delivered to cells by other techniques.
We have made a quantitative analysis of the uptake and infectivity of the vesicle-encapsulated RNA by using various ratios
of RNA copies per vesicle and by determining the percentage uptake of labelled lipid and RNA by HeLa cells.
Presented in the symposium on Gene Transfer, Differentiation and Neoplasia in Plant and Animal Cells at the 30th Annual Meeting
of the Tissue Culture Association, Seattle, Washington, June 10–14, 1979. This symposium was supported in part by Grant CA
26748 from the National Cancer Institute, DHEW, and Grant RD-67 from the American Cancer Society.
The research described here was supported by Grants AI-14042, CA-18527 and GM-18921 from the National Institute of Health
and IN-54P-16 from the American Cancer Society. 相似文献
8.
Laminin provides a better substrate than fibronectin for attachment,growth, and differentiation of 1003 embryonal carcinoma cells 总被引:3,自引:0,他引:3
Michel Y. Darmon 《In vitro cellular & developmental biology. Plant》1982,18(12):997-1003
Summary Culture of cells in hormonally defined media has allowed (a) the demonstration of physiological responses from cells usually
unable to express them in vitro and (b) the study of the effects on growth and differentiation of diffusible factors and attachment
factors. The embryonal carcinoma line 1003 forms multidifferentiated tumors in vivo but is unable to differentiate in vitro
when grown in serum-containing medium. In a defined medium containing insulin, transferrin, selenium, and fibronectin as attachment
factors, 1003 cells grow for several generations and differentiate into neurons and embryonic mesenchyme (Darmon et al., 1981,
Dev. Biol. 85: 463–473). In the present work the effects of fibronectin and laminin were compared. In the presence of laminin
the cells attached and spread better, grew faster, and could be plated at lower densities. Neurite extension was also better
under these conditions and most importantly, it was found that laminin induced an important formation of muscular tissue when
the cells had been seeded at low densities. Multinucleated myotubes could be stained with antibodies directed against embryonic
muscular myosin. Coating the dishes with polylysine or adding FGF or serum-spreading factor to the medium allowed growth of
low-density cultures with fibronectin instead of laminin but muscular differentiation was not detected under these conditions.
Addition of fibronectin to laminin-containing medium did not inhibit muscular differentiation.
Presented in the symposium on Plant and Animal Physiology in Vitro at the 33rd Annual Meeting of the Tissue Culture Association,
San Diego, California, June 6–10, 1982.
This research was supported in part by grants from the “Centre National de la Recherche Scientifique” (LA 269), the “Délégation
Générale à la Recherche Scientifique et Technique,” the Fondation pour la Recherche Médicale Fran?aise,” the “Institut National
de la Santé et de la Recherche Medicale,” the “Ligue Nationale Fran?aise centre le Cancer,” and the “Fondation André Meyer.”
This symposium was supported in part by the following organizations: Bellco Glass, Inc., California Branch of the Tissue Culture
Association, Collaborative Research, Hana Media, Hybridtech, K C Biological, Inc., and Millipore Corporation. 相似文献
9.
W. K. Whitten 《In vitro cellular & developmental biology. Plant》1979,15(1):19-22
Summary The genetic background and morphology of spontaneous mosaic hermaphrodites of mice have been described.
Presented in the formal symposium on Sexual Differentiation in Vitro and in Vivo at the 29th Annual Meeting of the Tissue
Culture Association, Denver, Colorado, June 4–8. 1978.
This work was supported by Grant HD 04083 from the National Institute of Child Health and Human Development. 相似文献
10.
The role of junctional communication in animal tissues 总被引:2,自引:0,他引:2
John D. Pitts 《In vitro cellular & developmental biology. Plant》1980,16(12):1049-1056
Summary Permeable intercellular junctions are a common feature of most animal tissues. These junctions allow the free exchange of
small ions and molecules between all the cells in coupled populations. Such limited syncytial interaction contributes to the
integration of individual cells into organized tissues.
Presented in the symposium on Molecular and Morphological Aspects of Cell-Cell Communication at the 31st Annual Meeting of
the Tissue Culture Association, St. Louis, Missouri, June 1–5, 1980.
This symposium was supported in part by Contract 263-MD-025754 from the National Cancer Institute and the Fogarty International
Center. 相似文献
11.
Dr. Richard A. Rifkind Paul A. Marks Arthur Bank Masaaiki Terada Roberta C. Reuben George M. Maniatis Eitan Fibach Uri Nudel Jane E. Salmon Yair Gazitt 《In vitro cellular & developmental biology. Plant》1978,14(1):155-161
Summary Studies are described employing two erythropoietic systems to elucidate regulatory mechanisms that control both normal erythropoiesis
and erythroid differentiation of transformed hemopoietic precursors. Evidence is provided suggesting that normal erythroid
cell precursors require erythropoietin as a growth factor that regulates the number of precursors capable of differentiating.
Murine erythroleukemia cells proliferate without need of erythropoietin; they show a variable, generally low, rate of spontaneous
differentiation and a brisk rate of erythropoiesis in response to a variety of chemical agents. Present studies suggest that
these chemical inducers initiate a series of events including cell surface related changes, alterations in cell cycle kinetics,
and modifications of chromatin and DNA structure which result in the irreversible commitment of these leukemia cells to erythroid
differentiation and the synthesis of red-cell-specific products.
Presented in the formal symposium on Mechanisms of Cellular Control at the 28th Annual Meeting of the Tissue Culture Association,
New Orleans, Louisiana, June 6–9, 1977.
These studies were supported in part by grants and contracts from the National Institutes of Health (GM-14552, CA-13696, CA-18314,
NO1-CB-4008 and NO1-CP-1008) and the National Science Foundation (NSF-PCM-75-08696). E.F. and R.C.R. are fellows of the Schultz
Foundation; A.B. was supported in part as an American Cancer Society Scholar; J.E.S. was supported by a USPHS Medical Scientist
Training Grant; and M.T. and G.M.M. are Hirschl Trust Scholars. 相似文献
12.
Summary Urogenital morphogenesis and cytodifferentiation are presented in the context of the epithelial-stromal interaction. The essential
role of stroma in these processes is reviewed.
Presented in the formal symposium on Sexual Differentiation in Vitro and in Vivo at the 29th Annual Meeting of the Tissue
Culture Association, Denver, Colorado, June 4–8, 1978.
The study was supported in part by Grant No. PDT-8 from the American Cancer Society, and Contract Grants N01-CP-55649 and
N01-CP-75875 from the National Cancer Institute. 相似文献
13.
D. Gospodarowicz G. Greenburg H. Bialecki B. R. Zetter 《In vitro cellular & developmental biology. Plant》1978,14(1):85-118
Summary The control of proliferation of mesoderm-derived cells by EGF and FGF has been examined taking, as an example, the vascular
endothelium. The mechanisms by which cell proliferation can be brought to a stop in vivo and in vitro have been reviewed.
Presented in the formal symposium on Mechanisms of Cellular Control at the 28th Annual Meeting of the Tissue Culture Association,
New Orleans, Louisiana, June 6–9, 1977.
This work was supported by Grants HL20-197 and HD 11082 from the National Institutes of Health, and VC-194 from the American
Cancer Society. B. R. Zetter was supported by Fellowship 5-F32-CA05149-02 from the U.S. Public Health Service. 相似文献
14.
The permeability of the cell-to-cell membrane channel and its regulation in mammalian cell junctions
Jean L. Flagg-Newton 《In vitro cellular & developmental biology. Plant》1980,16(12):1043-1048
Summary Mammalian cell-to-cell channels show polar permselective properties discriminating against negatively charged 14 ?-wide molecules
and are more restrictive than the channels of insect cell junctions. The channel permeability is modulated by conditions affecting
the concentration of intracellular ionic Ca: elevation of the external Ca load (B cells), treatment of cell cultures with
Ca-transporting ionophore (in the presence of external Ca, but not in its absence), treatment with a combination of cyanide
and iodoacetate, or with high levels of carbon dioxide, all cause depression of channel permeability. Treatment of cell cultures
with cyclic AMP or its more permeable derivative, dibutyryl cyclic AMP, produces increase in permeability. A similar channel
up regulation is observed upon elevation of the endogenous level of cyclic AMP by serum deprivation or lowering of cell density.
Presented in the symposium on Molecular and Morphological Aspects of Cell-Cell Communication at the 31st Annual Meeting of
the Tissue Culture Association, St. Louis, Missouri, June 1–5, 1980.
This symposium was supported in part by Contract 263-MD-025754 from the National Cancer Institute and the Fogarty International
Center.
This work was supported by grant number 5 R01 CA14464, awarded by the National Cancer Institute, DHEW. 相似文献
15.
E. F. Oakberg 《In vitro cellular & developmental biology. Plant》1979,15(1):41-49
Summary Follicles were classified on the basis of the number of layers of follicle cells, the presence and degree of development of
the zona pellucida, and the presence of an antrum. Formation of an antrum in follicles with less than 7 to 8 cell layers and/or
presence of necrotic cells was considered indicative of degeneration. When classified in this manner, the data suggest that
follicles and their contained oocytes are committed to either normal development or atresia by the time a third layer of granulosa
cells is formed.
Presented in the formal symposium on Sexual Differentiation in Vitro and in vivo at the 29th Annual Meeting of the Tissue
Culture Association, Denver, Colorado, June 4–8, 1978.
This research was sponsored by the Division of Biomedical and Environmental Research, U.S. Department of Energy, under Contract
W-7405-eng-26 with the Union Carbide Corporation. 相似文献
16.
Cell junctions and intercellular communication 总被引:1,自引:0,他引:1
J. -P. Revel S. B. Yancey D. J. Meyer B. Nicholson 《In vitro cellular & developmental biology. Plant》1980,16(12):1010-1017
Summary We have compared intercellular communication in normal and regenerating rat liver. Gap junctions are greatly reduced in size
and numbers 29 to 35 hr after hepatectomy, but we still find some 90% of hepatocytes coupled by electrophysiological criteria.
The spread of dyes such as carboxyfluorescein however is very limited in the regenerating organs as compared to the situation
in the controls. We show how the apparent discrepancies between morphological and physiological data can be reconciled. We
also present a summary of preliminary findings on the biosynthesis of gap junction protein and some of the conclusions one
can draw from the sequence of 58 amino acids at the amino terminal of the protein.
Presented in the symposium on Molecular and Morphological Aspects of Cell-Cell Communication at the 31st Annual Meeting of
the Tissue Culture Association, St. Louis, Missouri, June 1–5, 1980.
The original research described was supported by Grants GM 06965 and RR 07003 from the National Institute of Health, and funds
from the North-west Area Foundation. David Meyer and Barbara Yancey were the recipients of NIH postdoctoral fellowships (NS
06240 and AM05700). This symposium was supported in part by Contract 263-MD-025754 from the National Cancer Institute and
the Fogarty International Center. 相似文献
17.
Elliot L. Hertzberg 《In vitro cellular & developmental biology. Plant》1980,16(12):1057-1067
Summary Studies on gap junctions isolated from rat liver by a procedure that avoids exogenous proteolysis (Hertzberg, E. L.; Gilula,
N. B.; J. Biol. Chem. 254: 2138–2147; 1979) are described. The original isolation procedure was modified to increase the yield
and has been extended to the preparation of gap junctions from mouse and bovine liver. Peptide map studies showed that the
27,000-dalton polypeptides present in liver gap junction preparations from all three sources are homologous and are not derived
from other polypeptides of higher molecular weight that are observed in cruder preparations. Similar studies with lens fiber
junctions demonstrated no homology between liver and lens junction polypeptides. Antibodies to the lens junction polypeptide
did not cross-react with the liver gap junction polypeptide, further supporting this conclusion.
Presented in the symposium on Molecular and Morphological Aspects of Cell-Cell Communication at the 31st Annual Meeting of
the Tissue Culture Association, St. Louis, Missouri, June 1–5, 1980.
This symposium was supported in part by Contract 263-MD-025754 from the National Cancer Institute and the Fogarty International
Center.
Research in the laboratory was supported by grants to Dr. Gilula from the National Institute of Health (HL 16507 and GM 24753). 相似文献
18.
Birgit Rose 《In vitro cellular & developmental biology. Plant》1980,16(12):1029-1042
Summary Cells of organs and tissues commonly communicate directly with one another via permeable membrane junctions. Cell-to-cell
channels, spanning the width of both membranes of a junction, are thought to provide the pathways between the cytoplasms of
adjacent cells for the immediate exchange of ions and small molecules. We study these cell-to-cell channels in a cell model
system, the salivary gland ofChironomus. Using intracellularly injected fluorescent labelled peptides and oligosaccharides of various molecular dimensions as channel
permeability probes we find the channels to have a bore of about 2 nm. The channel permeability can be modulated and, in the
extreme, the channels can be closed under various experimental conditions. With the aid of the Ca2+-sensitive photoprotein aequorin as monitor of cytoplasmic free Ca2+ concentration, we show that a determining factor in this modulation of channel permeability is the cytoplasmic free Ca2+ concentration. Moreover, results obtained by injection of different-sized and different-labelled channel permeability probes
together with Ca2+ indicate that closure of the individual channels may occur in more than one step, i.e., by a graded reduction of channel
bore.
Presented in the symposium on Molecular and Morphological Aspects of Cell-Cell Communication at the 31st Annual Meeting of
the Tissue Culture Association, St. Louis, Missouri, June 1–5, 1980.
This symposium was supported, in part by Contract 263-MD-025754 from the National Cancer Institute and the Fogarty International
Center. This work was supported by NH Grants 5P1GM23911-07 and 5T32-6M07403-04. 相似文献
19.
E. Sidebottom 《In vitro cellular & developmental biology. Plant》1980,16(1):77-86
Summary The lecture reviews some aspects of the work on the analysis of malignancy that have been, and are now being, pursued in the
Dunn School. A brief outline of the early experiments that first demonstrated that the malignancy of mouse tumor cells can
be suppressed by the fusion with normal cells is given, and then two areas of current interest in the laboratory are described.
The first is an attempt to analyze the clinically important property of tumors to metastasize and the second is the work on
the isolation and identification of an abnormal membrane glycoprotein present in tumor cells. In addition the value of cell
fusion methods as a general test of hypotheses of malignancy is emphasized.
Presented in the symposium on Gene Transfer, Differentiation and Neoplasia in Plant and Animal Cells at the 30th Annual Meeting
of the Tissue Culture Association, Seattle, Washington, June 10–14, 1979. This symposium was supported in part by Grant CA
26748 from the National Cancer Institute, DHEW, and Grant RD-67 from the American Cancer Society. 相似文献
20.
Improved medium and culture conditions for clonal growth with minimal serum protein and for enhanced serum-free survival of Swiss 3T3 cells 总被引:21,自引:0,他引:21
Summary Improved culture conditions have been developed that will support clonal growth of Swiss mouse embryo 3T3 cells at concentrations
of serum protein as low as 125μg/ml. Survival of the cells under completely protein-free conditions also is enhanced greatly.
The improvements that made these results possible include: (a) use of medium MCDB 402, which was developed specifically for
Swiss 3T3 cells by adjusting the concentrations of all components of Dulbecco's modified Eagle's medium to optimum values
for clonal growth with minimal serum protein and by adding other nutrients such as trace elements and “nonessential” amino
acids that were not in the original formula; (b) use of culture surfaces that are coated with a positively charged polymer,
poly-d-lysine; and (c) use of gentle low temperature trypsinization technique that minimizes cellular damage and the need to neutralize
residual trypsin.
Portions of this work were reported at the Thirtieth Annual Meeting of the Tissue Culture Association in Seattle, Washington.
This work was supported by Grant CA-15305 from the National Cancer Institute 相似文献