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1.
斜纹夜蛾水通道蛋白1(AQP1)基因的克隆、分子特性和表达分析 总被引:1,自引:0,他引:1
水通道蛋白(aquaporin, AQP)是生物体中一种重要的跨膜通道蛋白, 它通过一些中性小分子化合物的运输, 从而参与了昆虫对食物中水分再吸收、抗寒和抗干燥等重要生理机制。为研究斜纹夜蛾中水通道蛋白的基因特征和时空表达特征, 本研究利用同源克隆和RACE技术获得了斜纹夜蛾水通道蛋白1(AQP1)基因的两个转录异构体, 并将其分别命名为SL-AQP1A (GenBank登录号: KC999953)和SL-AQP1B (GenBank登录号: KC999954),其中SL-AQP1B比SL-AQP1A在推导的编码区5′端连续性缺失81个碱基, 而其他序列完全一致; 同源分析显示推导的SL-AQP1与家蚕为代表的水通道蛋白1具有较高的同源性。拓扑学和三级结构模拟显示其有经典的6个全跨膜结构域、2个半跨膜结构域、2个保守的NPA (asparagine-proline-alanine, 天冬氨酸-脯氨酸-丙氨酸)结构基序及选择性水孔构件ar/R (aromatic arginine, 芳香族精氨酸)。qRT-PCR结果显示, SL-AQP1整体时空表达差异性十分明显, 并且SL-AQP1B的表达量显著高于SL-AQP1A; SL-AQP1在卵期和预蛹期有较高的表达量, 在中肠、马氏管、血淋巴、消化腺中有相对较高的表达量, 暗示其在斜纹夜蛾中存在重要的渗透压调节作用。本文结果为进一步研究水通道蛋白在斜纹夜蛾中的作用提供了一定的分子基础。 相似文献
2.
AbstractThe Grand Canonical Monte Carlo (GCMC) and molecular dynamics (MD) simulation methods were used to investigate the adsorption and diffusion properties of CH4 and CO2 in montmorillonite slit-nanopores. It is found that, both CH4 and CO2 could adsorb closely onto the pore surface, while different adsorption states occur for CH4 and CO2, respectively, in montmorillonite slit-nanopores. Competitive adsorption of CO2 over CH4 exists in montmorillonite slit-nanopores, especially at the lower pressures, which is attributed to the different interaction intensity between the CH4–CO2 molecules and the pore surface. The diffusion coefficients of CH4 and CO2 both decrease with the enhanced pressures, while the CO2 has a relative weak diffusion coefficient comparing with CH4. A well displacement of the residual CH4 by CO2 in montmorillonite slit-nanopores was investigated, which is found that the displacement efficiency increases with the enhanced bulk pressures. It was determined that, the CO2 can be captured and reserved in the montmorillonite slit-nanopores during the displacement, and the sequestration amount of CO2 gets enhanced with the bulk pressure increasing. This study provides micro-behaviours of CH4 and CO2 in montmorillonite slit-nanopores, for the purpose to give out useful guidance for enhancing shale gas extraction by injecting CO2. 相似文献
3.
Yoshikawa Y Kobayashi K Oishi S Fujii N Furuya T 《Bioorganic & medicinal chemistry letters》2012,22(6):2146-2150
CXCR4 is a G-protein coupled receptor that is associated with many diseases such as breast cancer metastasis, HIV infection, leukemic disease and rheumatoid arthritis, and is thus considered an attractive drug target. Previously, we identified a cyclic pentapeptide, FC131, that is a potent antagonist for CXCR4. In this study, we constructed a three dimensional model of the CXCR4-FC131 complex. To investigate the backbone flexibility of FC131, we performed molecular dynamics simulations of FC131 based on the NMR structure of FC131, and obtained snapshot structures from the trajectories which were used to model the docking pose of FC131 into CXCR4. Our final model of the CXCR4-FC131 complex is partially different from the X-ray crystal structure of CXCR4-CVX15 and suggests water-mediated interactions. Nevertheless, this docking pose is consistent with the experimental data. We believe our model will aid in the discovery and development of small-molecule antagonists for CXCR4. 相似文献
4.
Syed Shan‐e‐Ali Zaidi Rubab Zahra Naqvi Muhammad Asif Susan Strickler Sara Shakir Muhammad Shafiq Abdul Manan Khan Imran Amin Bharat Mishra M. Shahid Mukhtar Brian E. Scheffler Jodi A. Scheffler Lukas A. Mueller Shahid Mansoor 《Plant biotechnology journal》2020,18(3):691-706
Cultivated cotton (Gossypium hirsutum) is the most important fibre crop in the world. Cotton leaf curl disease (CLCuD) is the major limiting factor and a threat to textile industry in India and Pakistan. All the local cotton cultivars exhibit moderate to no resistance against CLCuD. In this study, we evaluated an exotic cotton accession Mac7 as a resistance source to CLCuD by challenging it with viruliferous whiteflies and performing qPCR to evaluate the presence/absence and relative titre of CLCuD‐associated geminiviruses/betasatellites. The results indicated that replication of pathogenicity determinant betasatellite is significantly attenuated in Mac7 and probably responsible for resistance phenotype. Afterwards, to decipher the genetic basis of CLCuD resistance in Mac7, we performed RNA sequencing on CLCuD‐infested Mac7 and validated RNA‐Seq data with qPCR on 24 independent genes. We performed co‐expression network and pathway analysis for regulation of geminivirus/betasatellite‐interacting genes. We identified nine novel modules with 52 hubs of highly connected genes in network topology within the co‐expression network. Analysis of these hubs indicated the differential regulation of auxin stimulus and cellular localization pathways in response to CLCuD. We also analysed the differential regulation of geminivirus/betasatellite‐interacting genes in Mac7. We further performed the functional validation of selected candidate genes via virus‐induced gene silencing (VIGS). Finally, we evaluated the genomic context of resistance responsive genes and found that these genes are not specific to A or D sub‐genomes of G. hirsutum. These results have important implications in understanding CLCuD resistance mechanism and developing a durable resistance in cultivated cotton. 相似文献
5.
Nonsymbiotic hemoglobins (nsHbs) are widespread in plants including bryophytes. Bryophytes (such as mosses) are among the oldest land plants, thus an analysis of a bryophyte nsHb is of interest from an evolutionary perspective. However, very little is known about bryophyte nsHbs. Here, we report the cloning and characterization of an nshb gene (cerhb) from the moss Ceratodon purpureus. Sequence analysis showed that cerhb is interrupted by 3 introns in identical position as all known plant nshb genes, which suggests that the ancestral nshb gene was interrupted by 3 introns. Expression analysis showed that cerhb expresses in protonemas and gametophytes growing in normal conditions and that it overexpresses in protonemas subjected to osmotic (sucrose), heat-shock, cold-, and nitrate-stress conditions. Also, modeling of the Ceratodon nsHb (CerHb) tertiary structure suggests that CerHb is hexacoordinate and that it binds O(2) with high affinity. Comparative analysis of the predicted CerHb with native rice Hb1 and soybean leghemoglobin a structures revealed that the major evolutionary changes that probably occurred during the evolution of plant Hbs were 1) a hexacoordinate to pentacoordinate transition at the heme prosthetic group, 2) a length decrease at the CD-loop and N- and C-termini regions, and 3) the compaction of the protein into a globular structure. 相似文献
6.
Hitomi Kawaida Kohki Ohba Yuhki Koutake Hiroshi Shimizu Hidenori Tachida Yoshitaka Kobayakawa 《Molecular phylogenetics and evolution》2013,66(3):906-914
Although many physiological studies have been reported on the symbiosis between hydra and green algae, very little information from a molecular phylogenetic aspect of symbiosis is available. In order to understand the origin and evolution of symbiosis between the two organisms, we compared the phylogenetic relationships among symbiotic green algae with the phylogenetic relationships among host hydra strains. To do so, we reconstructed molecular phylogenetic trees of several strains of symbiotic chlorella harbored in the endodermal epithelial cells of viridissima group hydra strains and investigated their congruence with the molecular phylogenetic trees of the host hydra strains. To examine the species specificity between the host and the symbiont with respect to the genetic distance, we also tried to introduce chlorella strains into two aposymbiotic strains of viridissima group hydra in which symbiotic chlorella had been eliminated in advance. We discussed the origin and history of symbiosis between hydra and green algae based on the analysis. 相似文献
7.
Giffard-Mena I Boulo V Aujoulat F Fowden H Castille R Charmantier G Cramb G 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2007,148(2):430-444
Euryhaline fish possess the ability to compensate for environmental salinity changes through hydro-mineral regulation. A number of proteins have been studied in order to understand water and ion exchanges, known as fish osmoregulation. Sea-bass (Dicentrarchus labrax) cDNA sequences encoding a homologue of mammalian aquaporin (termed AQP1) and a homologue of mammalian aquaglyceroporin (termed AQP3) have been isolated and sequenced. The aquaporin amino acid sequences share respectively more than 60% and 65% identity with other known aquaporins. We have shown that salinity influences aquaporin expression levels in the gill, kidney and digestive tract, the main osmoregulatory organs. AQP1 may have a major osmoregulatory role in water transport in kidney and gut in SW-acclimated fish, whereas AQP3 could be implicated in gill water transport in FW-acclimated fish. 相似文献
8.
Shangang Jia Yan Zhou Chuzhao Lei Ru Yao Zhiying Zhang Xingtang Fang Hong Chen 《遗传学报》2010,37(3):173-180
The domestication of cattle fuelled the development of agricultural society in the history of human being.The evolution and genetic relationship of cattle can be elucidated by investigating the variation of mitochondrial DNA (mtDNA) D-loop sequence.In this study,we built a cattle phylogeny with a pool of 856 individual D-loop sequences,of which 264 Chinese cattle D-loop sequences were obtained in this study (141 ones were first analyzed,and 123 were first submitted) and the rest sequences of cattle from six Asian countries (Japan,Korea,Mongolia,Nepal,India and China) were retrieved from GenBank.Our results indicated that cattle from six Asian countries fell into three clades,Bos taurus (taurine),Bos indicus (zebu) and yak.Four main haplogroups T1A,T2,T3 (including T3A and T3B) and T5 were found in taurine,and two haplogroups I1 and I2 in zebu.Furthermore,we found that I1 and I2 haplogroups were separated by four variable sites rather than five ones and four haplogroups or sub-haplogroups of T1A,T3A,T3B and T5 were found for the first time in these Asian cattle.These data brought us a new insight into cattle's genetic structure in these six Asian countries.The geographical distribution of haplogroups was also outlined to provide systematic information on cattle genetic resources. 相似文献
9.
《Acta Genetica Sinica》2010,(3)
The domestication of cattle fuelled the development of agricultural society in the history of human being.The evolution and genetic relationship of cattle can be elucidated by investigating the variation of mitochondrial DNA(mtDNA) D-loop sequence.In this study,we built a cattle phylogeny with a pool of 856 individual D-loop sequences,of which 264 Chinese cattle D-loop sequences were obtained in this study(141 ones were first analyzed,and 123 were first submitted) and the rest sequences of cattle from six A... 相似文献
10.
《生物化学与生物物理学报:生物膜》2021,1863(12):183772
NMOSD is a rare but severe relapsing remitting demyelinating disease that affects both adults and children. Most patients have pathogenic antibodies that target the central nervous system AQP4 protein. This review provides an update on our current understanding of the disease pathophysiology and describes the clinical, paraclinical features and therapeutic management of the disease. 相似文献
11.
Andreas Ritter Nina-Naomi Kreis Frank Louwen Linda Wordeman 《Critical reviews in biochemistry and molecular biology》2016,51(4):228-245
Chromosome stability is ensured by precisely fine-tuned dynamics of mitotic spindles, which are controlled by a network of various microtubule-associated and interacting proteins including the kinesin-13 family. The best characterized member of this family is the mitotic centromere-associated kinesin (MCAK). By efficiently depolymerizing microtubules, MCAK influences various key events during mitosis. MCAK itself is regulated by its interaction partners, its intrinsic conformation switch and the phosphorylation of mitotic kinases like Aurora A/B, cyclin-dependent kinase 1 and Polo-like kinase 1. Perturbing its regulation alters MCAK’s conformation, catalytic activity, subcellular localization and stability, leading further to mitotic defects in spindle formation and chromosome movement. Indeed, MCAK is aberrantly regulated in various cancer types, which is linked to increased invasiveness, metastasis and drug resistance. In the current review, we summarize recently published data concerning MCAK, correlate its conformation changes with its depolymerization activity and function, propose a model of its regulation by multiple mitotic kinases and highlight its potential involvement in oncogenesis and drug resistance. 相似文献
12.
Molecular characterization, chromosomal and expression patterns of three aquaglyceroporins (AQP3, 7, 9) from pig 总被引:1,自引:0,他引:1
Li X Lei T Xia T Chen X Feng S Chen H Chen Z Peng Y Yang Z 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2008,149(3):468-476
As one subgroup of aquaporin, aquaglyceroporin including AQP3, 7, 9, 10 facilitates glycerol transport as well as water transport. In this study, we cloned the full length coding sequences of porcine (Sus scrofa) AQP3, 7 and 9 and the genomic sequence of AQP3 including 6 exons and 5 introns. Additionally, as a first step toward understanding the regulatory mechanisms of AQP9 in pig, we cloned and analyzed the upstream genomic sequence of the ATG translation initiation codon and found two negative insulin response elements (TGTTTTC and TATTTTG.), glucocorticoid-responsive elements, several CCAAT enhancer binding protein (C/EBP) sites, hepatocyte nuclear factor (HNF) sites, and NF-kappaB sites in this region. Subsequently, semi-quantitative analysis showed that AQP3 selectively expressed in spleen, stomach, kidney and lung. AQP7 and AQP9 were ubiquitously detected in all tissues examined and highly expressed in adipose tissue and liver, respectively. Finally, both AQP3 and AQP7 were assigned to chromosome 10q while AQP9 was mapped to chromosome 1q. This is the first report of molecular characterization of aquaglyceroporin in pig, which provides basic observations useful for future assessing and characterizing the role of aquaglyceroporin. 相似文献
13.
《Structure (London, England : 1993)》2023,31(5):511-517.e3
14.
Functional characterization of the T4 DNA ligase: a new insight into the mechanism of action. 下载免费PDF全文
ATP-dependent DNA ligases are essential enzymes in both DNA replication and DNA repair processes. Here we report a functional characterization of the T4 DNA ligase. One N-terminal and two C-terminal deletion mutants were expressed in Escherichia coli as histidine- tagged proteins. An additional mutant bore a substitution of Lys159 in the active site that abolished ATP binding. All the proteins were tested in biochemical assays for ATP-dependent self-adenylation, DNA binding, nick joining, blunt-end ligation and AMP- dependent DNA relaxation. From this analysis we conclude that binding to DNA is mediated by sequences at both protein ends and plays a key role in the reaction. The enzyme establishes two different complexes with DNA: (i) a transient complex (T.complex) involving the adenylated enzyme; (ii) a stable complex (S.complex) requiring the deadenylated T4 DNA ligase. The formation of an S. complex seems to be relevant during both blunt-end ligation and DNA relaxation. Moreover the inactive His-K159L substitution mutant, although unable to self-adenylate, still possesses AMP-dependent DNA nicking activity. 相似文献
15.
Influence of axial ligands, MeCN, H2O, py, and piperidine (pip), on distortion of (2,3,5,10,12,13,15,20-octaphenylporphinato)chromium(III), [Cr(OPP)]+, was investigated by X-ray crystallography and UV-vis and ESR spectroscopies. In crystal structures of [Cr(OPP)(MeCN)(H2O)]ClO4 · MeCN and [Cr(OPP)(H2O)2]ClO4 · 3THF, the OPP2− ligand had a planar structure. On the other hand, crystal structures of [Cr(OPP)(pip)2]ClO4 · 2CH2Cl2 and [Cr(OPP)(py)2]ClO4 exhibited a waved structure and a saddle-shaped structure with ruffling, respectively. In the UV-vis spectrum of [Cr(OPP)(py)2]ClO4 in CH2Cl2, the large red shift of the Soret band was observed. Furthermore, the small D value of 0.10 cm−1 was obtained for [Cr(OPP)(py)2]ClO4 from the ESR spectrum in frozen 1,2-dichloroethane solution at 5 K. These results suggest that the OPP2− ligand is distorted both in solid and in solution, and that the axial ligand would exert some effects on the porphyrin distortion. 相似文献
16.
Aharon G. Fleury Eva M. MacLennan Rylan J. Command Francis Juanes 《Journal of fish biology》2021,99(2):596-606
The reproductive biology of Pacific hagfish Eptatretus stoutii (Lockington, 1878) and black hagfish Eptatretus deani (Evermann & Goldsborough, 1907) was assessed using current and historical data. Our results found that the reproductive characteristics of both hagfish species reflect those of K-selected species, which tend to live long and exhibit slow growth rates, low fecundity (approximately 20 eggs per female) and late maturity. Additionally, females of both species commence maturation prior to males. This study provides a population profile for both species of hagfish, but further assessments are needed to effectively manage a sustainable hagfish fishery. 相似文献
17.
Revealing selectivity mechanism of cyclin-dependent kinases (CDK) and their inhibitors is an important issue to develop potential anticancer drugs. The substituted 4-(Pyrazol-4-yl)-pyrimidines are potent inhibitors of CDK4 but not of the highly homologous CDK2. In order to reveal the inhibitory selectivity of these inhibitors to CDK4 over CDK2, we select one of substituted 4-(Pyrazol-4-yl)-pyrimidines as a representative (marked as A1 hereunder) and perform molecular docking, molecular dynamics simulations and binding free energy analysis for CDK4/A1 and CDK2/A1, respectively. The electrostatic and van der Waals (vdW) interactions of the A1 inhibitor with CDK4/CDK2 are discussed. The computed binding free energies based on the MM-PBSA method are consistent with experimental bioactivity ranking of A1 inhibitor to CDK4/CDK2. On the other hand, the conformational characteristics of CDK2 and CDK4 induced by A1 inhibitor are analysed and revealed. Results demonstrate that the vdW interactions considerably contribute to binding of CDK4/CDK2 with A1 inhibitor and are similar in size. The hydrogen bonding between A1 inhibitor and CDK4/CDK2 is considerably favourable to the binding, in which the hydrogen bond between the NH group of the pyrazole group of A1 and the residue Asp158 of CDK4 plays a crucial role in inhibitory selectivity of A1 inhibitor to CDK4 over CDK2. The electrostatic interaction energy differences between the corresponding residues of CDK4/A1 and CDK2/A1 confirm the above inference. The conformational changes of CDK2 and CDK4 induced by A1 inhibitor influence the selectivity of A1 inhibitor to CDK4/CDK2. 相似文献
18.
19.
A pair of novel neutral glycosphingolipids (Ngsls) has been identified in bovine brain. Their mobilities on thin layer chromatography were slightly different from a standard pentaglycosylceramide (nLcOse(5)Cer from bovine erythrocytes). The compounds were purified to homogeneity by column chromatography. Their fatty acid and base compositions, their monosaccharide compositions and sugar linkage positions were determined by gas-liquid chromato-graphy/mass spectrometry. Carbohydrate sequence analy-sis by(1)H NMR spectroscopy and stepwise exoglyco-sidase digestion indicated the following pentaglycosyl structure for the oligosaccharide moiety of both Ngsls: GalNAcbeta1-4Galbeta1-3GalNAcbeta1-4Galbeta1-4Gl c. The two Ngsls (abbreviated as IV(4)GalNAcGgOse(4)Cer or GalNAc-GA1), differ in their ceramide compositions, having d18:0 and d18:1 sphingosine as their long chain bases. A monospecific polyclonal anti-GalNAc-GA1 antibody, prepared in rabbit and purified by affinity chromatography, stained the neurons of cerebral cortex and cerebellum including Purkinje cells in adult rat brain, indicating that the novel GalNAc-GA1 is associated with cerebellar and other neurons in vertebrate central nervous system. 相似文献
20.
The current taxonomy on the haemococcidia establishes that the two genera of protozoan parasites that integrate the family Lankesterellidae are Lankesterella and Schellackia. However, the phylogeny of these genera, as well as the other coccidia, remains unresolved. In this sense, the use of type and described species is essential for the resolution of systematic conflicts. In this study, we molecularly characterize the type species of the genus Schellackia, that is, S. bolivari from Europe and also a described species of the same genus from Asia. At the same time, we contribute with the molecular characterization of another species of the genus Lankesterella. All this put together supports the polyphyly of the family Lankesterellidae. Therefore, we propose the resurrection of the zoological family, Schellackiidae Grassé 1953 , to include species within the genus Schellackia. 相似文献