共查询到20条相似文献,搜索用时 15 毫秒
1.
Borrego A Peters LC Jensen JR Ribeiro OG Koury Cabrera WH Starobinas N Seman M Ibañez OM De Franco M 《Microbes and infection / Institut Pasteur》2006,8(12-13):2766-2771
Two lines of mice selected to produce maximal (AIRmax) or minimal (AIRmin) acute inflammatory reactions (AIR) differ in their susceptibility to infection by Salmonella enterica serotype Typhimurium (S. Typhimurium). The LD(50) for AIRmax mice is 1000 times higher than that observed for AIRmin mice, and higher frequencies of Slc11a1 alleles (known to confer either resistance (R) or high susceptibility (S) to S. Typhimurium) were consistently found in AIRmax and AIRmin mouse lines, respectively. In order to evaluate the effect of the quantitative trait loci (QTL) segregated in AIRmax and AIRmin mice on Slc11a1 dependent susceptibility to S. Typhimurium, the R and S alleles were fixed in homozygosity in AIRmax and AIRmin backgrounds by genotype assisted breedings. These new lines were named AIRmax(RR), AIRmax(SS), AIRmin(RR), and AIRmin(SS). Acute inflammation of Slc11a1(RR) animals was more severe in comparison to their Slc11a1(SS) counterparts, implicating Slc11a1 (or other linked genes) in AIR regulation. The LD(50) of S. Typhimurium was 800-times higher for AIRmax(SS) than for AIRmin(SS), demonstrating that AIR QTL can act as modifiers of the Slc11a1(SS) susceptibility gene. Four microsatellite markers for S. Typhimurium susceptibility QTL described in other mouse lines showed specific allele fixation in AIRmax or AIRmin mice, suggesting that these chromosomal regions also segregate with inflammatory phenotypes. 相似文献
2.
L. A. Pershina E. P. Devyatkina L. I. Belova N. V. Trubacheeva V. S. Arbuzova L. A. Kravtsova 《Russian Journal of Genetics》2009,45(10):1223-1229
Two alloplasmic wheat-barley substitution lines were studied: a line replaced at three pairs of chromosomes 1H
mar
(1B), 5H
mar
(5D), and 7H
mar
(7D), and the disomic-substituted line 7H
mar
(7D). The lines were constructed on the basis of individual plants from BC1F8 and BC2F6 progeny of barley-wheat hybrids (H. marinum subsp. gussoneanum Hudson (= H. geniculatum All.) (2n = 28) × T. aestivum L.) (2n = 42) (Pyrotrix 28), respectively. Moreover, the alloplasmic wheat-barley ditelosomic addition line 7HL
mar
isolated among plants from the BC1F6 progeny of a barley-wheat amphiploid was studied, which in this work corresponds to BC2F10 and BC2F11 progeny. It was ascertained that when grown in the field, these alloplasmic lines manifest stable self-fertility. Plants
of the given lines are characterized by low height, shortened ears, the fewer number of stems and ears, and of spikelets in
the ear, by decreased grain productivity and weight of 1000 grains, in comparison with the common wheat cultivar Pyrotrix
28. The inhibition of trait expression in alloplasmic wheat-barley substitution and addition lines may be connected not only
with the influence of wild barley chromosomes functioning in the genotypic environment of common wheat, but also with the
effect of the barley cytoplasm. The alloplasmic line with substitution of chromosomes 1H
mar
(1B), 5H
mar
(5D), and 7H
mar
(7D) or the alloplasmic line 5HL
mar
with ditelosomic addition have, in comparison with the common wheat cultivar Pyrotrix 28, an increased grain protein content,
which is explained by the effect of wild barley H. marinum subsp. gussoneanum chromosomes. 相似文献
3.
Zhang YX Wang Q Jiang L Liu LL Wang BX Shen YY Cheng XN Wan JM 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(8):1591-1604
Rice stripe disease, caused by rice stripe virus (RSV), is one of the most serious diseases in temperate rice-growing areas.
In the present study, we performed quantitative trait locus (QTL) analysis for RSV resistance using 98 backcross inbred lines
derived from the cross between the highly resistant variety, Kasalath, and the highly susceptible variety, Nipponbare. Under
artificial inoculation in the greenhouse, two QTLs for RSV resistance, designated qSTV7 and qSTV11
KAS
, were detected on chromosomes 7 and 11 respectively, whereas only one QTL was detected in the same location of chromosome
11 under natural inoculation in the field. The stability of qSTV11
KAS
was validated using 39 established chromosome segment substitution lines. Fine mapping of qSTV11
KAS
was carried out using 372 BC3F2:3 recombinants and 399 BC3F3:4 lines selected from 7,018 BC3F2 plants of the cross SL-234/Koshihikari. The qSTV11
KAS
was localized to a 39.2 kb region containing seven annotated genes. The most likely candidate gene, LOC_Os11g30910, is predicted
to encode a sulfotransferase domain-containing protein. The predicted protein encoded by the Kasalath allele differs from
Nipponbare by a single amino acid substitution and the deletion of two amino acids within the sulfotransferase domain. Marker-resistance
association analysis revealed that the markers L104-155 bp and R48-194 bp were highly correlated with RSV resistance in the
148 landrace varieties. These results provide a basis for the cloning of qSTV11
KAS
, and the markers may be used for molecular breeding of RSV resistant rice varieties. 相似文献
4.
Quantitative characteristics of photosynthetic electron transport were evaluated in vivo on the basis of the multi-exponential
analysis of OJIP fluorescence transients induced by saturating actinic light. The OJIP fluorescence curve F(t), measured in Chlamydomonas reinhardtii cells, was transformed into the (1 − F
O/F(t)) × (F
V
/F
M)−1 transient, which is shown to relate to PS 2 closure. We assumed that kinetics of PS 2 closure during OJIP rise reflects time-separated
processes related to the establishment of redox equilibrium at the PS 2 acceptor side (OJ), PQ pool (JI), and beyond Cyt b/f
(IP). Three-exponential fitting was applied to (1 − F
O/F(t)) × (F
V
/F
M)−1 transient to obtain lifetimes and amplitudes of the OJ, JI, and IP components of PS 2 closure, which were used to calculate
overall rates of reduction and re-oxidation of the PS 2 acceptor side, PQ pool, and intermediates beyond Cyt b/f complex.
The results, obtained in the presence of inhibitors, oxidative reagents, and under different stress conditions prove the suggested
model and characterize the introduced parameters as useful indicators of photosynthetic function. 相似文献
5.
Soengas P Hand P Vicente JG Pole JM Pink DA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(4):637-645
Resistance to six known races of black rot in crucifers caused by Xanthomonas campestris pv. campestris (Pammel) Dowson is absent or very rare in Brassica oleracea (C genome). However, race specific and broad-spectrum resistance (to type strains of all six races) does appear to occur
frequently in other brassica genomes including B. rapa (A genome). Here, we report the genetics of broad spectrum resistance in the B. rapa Chinese cabbage accession B162, using QTL analysis of resistance to races 1 and 4 of the pathogen. A B. rapa linkage map comprising ten linkage groups (A01–A10) with a total map distance of 664 cM was produced, based on 223 AFLP bands
and 23 microsatellites from a F2 population of 114 plants derived from a cross between the B. rapa susceptible inbred line R-o-18 and B162. Interaction phenotypes of 125 F2 plants were assessed using two criteria: the percentage of inoculation sites in which symptoms developed, and the severity
of symptoms per plant. Resistance to both races was correlated and a cluster of highly significant QTL that explained 24–64%
of the phenotypic variance was located on A06. Two additional QTLs for resistance to race 4 were found on A02 and A09. Markers
closely linked to these QTL could assist in the transference of the resistance into different B. rapa cultivars or into B. oleracea. 相似文献
6.
Borovsky Y Paran I 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(4):657-665
We previously identified fs10.1 as a major QTL controlling fruit shape (index of length to width) in an interspecific F2 cross of Capsicum annuum (round fruit) × C. chinense (elongated fruit) in pepper. To more precisely map and characterize the QTL, we constructed near-isogenic lines for fs10.1 and mapped it in a BC4F2 population. In this population, fs10.1 segregated as a Mendelian locus and mapped 0.3 cM away from the closest molecular marker. We further verified the effect
of fs10.1 in an F2 population from an independent cross between elongated- and conical-fruited parents. To identify additional allelic variation
at fruit shape loci, we screened an EMS-mutagenized population of the blocky-fruited cv. Maor and identified the mutant E-1654
with elongated fruit. This fruit shape mutation was mapped to the fs10.1 region and was determined to be allelic to the QTL. By measuring fruit shape of near-isogenic lines for fs10.1 during fruit development, we found that the shape of the fruit is determined primarily in the first 2 weeks after anthesis.
Histological measurements of cell size and cell shape in pericarp sections of fruits of the isogenic lines throughout fruit
development indicated that the shape of the fruit is determined primarily by cell shape and that the development of fruit
shape is correlated with cell shape. 相似文献
7.
Micic Z Hahn V Bauer E Schön CC Knapp SJ Tang S Melchinger AE 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(7):1474-1484
In many sunflower-growing regions of the world, Sclerotinia sclerotiorum (Lib.) de Bary is the major disease of sunflower (Helianthus annuus L.). In this study, we mapped and characterized quantitative trait loci (QTL) involved in resistance to S. sclerotiorum midstalk rot and two morphological traits. A total of 351 F3 families developed from a cross between a resistant inbred line from the germplasm pool NDBLOS and the susceptible line CM625 were assayed for their parental F2 genotype at 117 codominant simple sequence repeat markers. Disease resistance of the F3 families was screened under artificial infection in field experiments across two sowing times in 1999. For the three resistance traits (leaf lesion, stem lesion, and speed of fungal growth) and the two morphological traits, genotypic variances were highly significant. Heritabilities were moderate to high (h2=0.55–0.89). Genotypic correlations between resistance traits were highly significant (P<0.01) but moderate. QTL were detected for all three resistance traits, but estimated effects at most QTL were small. Simultaneously, they explained between 24.4% and 33.7% of the genotypic variance for resistance against S. sclerotiorum. Five of the 15 genomic regions carrying a QTL for either of the three resistance traits also carried a QTL for one of the two morphological traits. The prospects of marker-assisted selection (MAS) for resistance to S. sclerotiorum are limited due to the complex genetic architecture of the trait. MAS can be superior to classical phenotypic selection only with low marker costs and fast selection cycles. 相似文献
8.
Runli He Zhijian Chang Zujun Yang Zongying Yuan Haixian Zhan Xiaojun Zhang Jianxia Liu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(6):1173-1180
Powdery mildew resistance from Thinopyrum intermedium was introgressed into common wheat (Triticum aestivum L.). Genetic analysis of the F1, F2, F3 and BC1 populations from powdery mildew resistant line CH5025 revealed that resistance was controlled by a single dominant allele.
The gene responsible for powdery mildew resistance was mapped by the linkage analysis of a segregating F2 population. The resistance gene was linked to five co-dominant genomic SSR markers (Xcfd233, Xwmc41, Xbarc11, Xgwm539 and Xwmc175) and their most likely order was Xcfd233–Xwmc41–Pm43–Xbarc11–Xgwm539–Xwmc175 at 2.6, 2.3, 4.2, 3.5 and 7.0 cM, respectively. Using the Chinese Spring nullisomic-tetrasomic and ditelosomic lines, the
polymorphic markers and the resistance gene were assigned to chromosome 2DL. As no powdery mildew resistance gene was previously
assigned to chromosome 2DL, this new resistance gene was designated Pm43. Pm43, together with the identified closely linked markers, could be useful in marker-assisted selection for pyramiding powdery
mildew resistance genes.
Runli He and Zhijian Chang contributed equally to this work. 相似文献
9.
10.
Voorrips RE Finkers R Sanjaya L Groenwold R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(6):1275-1282
Anthracnose fruit rot is an economically important disease that affects pepper production in Indonesia. Strong resistance to two causal pathogens, Colletotrichum gloeosporioides and C. capsici, was found in an accession of Capsicum chinense. The inheritance of this resistance was studied in an F2 population derived from a cross of this accession with an Indonesian hot pepper variety (Capsicum annuum) using a quantitative trait locus (QTL) mapping approach. In laboratory tests where ripe fruits were artificially inoculated with either C. gloeosporioides or C. capsici, three resistance-related traits were scored: the infection frequency, the true lesion diameter (averaged over all lesions that actually developed), and the overall lesion diameter (averaged over all inoculation points, including those that did not develop lesions). One main QTL was identified with highly significant and large effects on all three traits after inoculation with C. gloeosporioides and on true lesion diameter after inoculation with C. capsici. Three other QTL with smaller effects were found for overall lesion diameter and true lesion diameter after inoculation with C. gloeosporioides, two of which also had an effect on infection frequency. Interestingly, the resistant parent carried a susceptible allele for a QTL for all three traits that was closely linked to the main QTL. The results with C. capsici were based on less observations and therefore less informative. Although the main QTL was shown to have an effect on true lesion diameter after inoculation with C. capsici, no significant QTL were identified for overall lesion diameter or infection frequency. 相似文献
11.
Kai H Takata K Tsukazaki M Furusho M Baba T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(3):577-583
PK23-2, a line of six-rowed barley (Hordeum vulgare L.) originating from Pakistan, has resistance to Japanese strains I and III of the barley yellow mosaic virus (BaYMV). To
identify the source of resistance in this line, reciprocal crosses were made between the susceptible cultivar Daisen-gold
and PK23-2. Genetic analyses in the F1 generation, F2 generation, and a doubled haploid population (DH45) derived from the F1 revealed that PK23-2 harbors one dominant and one recessive resistance genes. A linkage map was constructed using 61 lines
of DH45 and 127 DNA markers; this map covered 1268.8 cM in 10 linkage groups. One QTL having a LOD score of 4.07 and explaining
26.8% of the phenotypic variance explained (PVE) for resistance to BaYMV was detected at DNA marker ABG070 on chromosome 3H.
Another QTL having a LOD score of 3.53 and PVE of 27.2% was located at marker Bmag0490 on chromosome 4H. The resistance gene
on chromosome 3H, here named Rym17, showed dominant inheritance, whereas the gene on chromosome 4H, here named rym18, showed recessive inheritance in F1 populations derived from crosses between several resistant lines of DH45 and Daisen-gold. The BaYMV recessive resistance
genes rym1, rym3, and rym5, found in Japanese barley germplasm, were not allelic to rym18. These results revealed that PK23-2 harbors two previously unidentified resistance genes, Rym17 on 3H and rym18 on 4H; Rym17 is the first dominant BaYMV resistance gene to be identified in primary gene pool. These new genes, particularly dominant
Rym17, represent a potentially valuable genetic resource against BaYMV disease. 相似文献
12.
Three QTLs for <Emphasis Type="Italic">Botrytis cinerea</Emphasis> resistance in tomato 总被引:2,自引:0,他引:2
Finkers R van den Berg P van Berloo R ten Have A van Heusden AW van Kan JA Lindhout P 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(4):585-593
Tomato (Solanum lycopersicum) is susceptible to grey mold (Botrytis cinerea). Partial resistance to this fungus was identified in accessions of wild relatives of tomato such as S. habrochaites LYC4. In order to identify loci involved in quantitative resistance (QTLs) to B. cinerea, a population of 174 F2 plants was made originating from a cross between S. lycopersicum cv. Moneymaker and S. habrochaites LYC4. The population was genotyped and tested for susceptibility to grey mold using a stem bioassay. Rbcq1, a QTL reducing lesion growth (LG) and Rbcq2, a QTL reducing disease incidence (DI) were identified. Rbcq1 is located on Chromosome 1 and explained 12% of the total phenotypic variation while Rbcq2 is located on Chromosome 2 and explained 15% of the total phenotypic variation. Both QTL effects were confirmed by assessing
disease resistance in two BC2S1 progenies segregating for either of the two QTLs. One additional QTL, Rbcq4 on Chromosome 4 reducing DI, was identified in one of the BC2S1 progenies. F2 individuals, homozygous for the Rbcq2 and Rbcq4 alleles of S. habrochaites showed a reduction of DI by 48%. QTLs from S. habrochaites LYC4 offer good perspectives for breeding B. cinerea resistant tomato cultivars.
Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users. 相似文献
13.
Ioannis?M.?Stylianou Julian?K.?Christians Peter?D.?Keightley Lutz?Bünger Michael?Clinton Grahame?Bulfield Simon?Horvat
Obesity is proving to be a serious health concern in the developed world as well as an unwanted component of growth in livestock production. While recent advances in genetics have identified a number of monogenic causes of obesity, these are responsible for only a small proportion of human cases of obesity. By divergent selection for high and low fat content over 60 generations, we have created Fat (F) and Lean (L) lines of mice that represent a model of polygenic obesity similar to the situation in human populations. From previous crosses of these lines, four body fat quantitative trait loci (QTL) were identified. We have created congenic lines (Fchr15L), by recurrent marker-assisted backcrossing, to introgress the QTL region with the highest LOD score, Fob3 on Chr 15, from the L-Iine into the F-line background. We have further mapped this QTL by progeny testing of recombinants, produced from crosses between the F-line and congenic Fchrl5L mice, showing that the Fob3 QTL region is a composite of at least two smaller effect QTL—the proximal QTL Fob3a is a late-onset obesity QTL, whereas the distal Fob3b is an early-onset obesity QTL. 相似文献
14.
Eriko Oikawa Shohei Takuno Atsushi Izumita Koji Sakamoto Hideaki Hanzawa Hiroyasu Kitashiba Takeshi Nishio 《Molecular breeding : new strategies in plant improvement》2011,28(1):1-12
In F1 hybrid breeding of Brassica vegetables utilizing the self-incompatibility system, identification of S genotypes in breeding lines is required. In the present study, we developed S-tester lines of 87 S haplotypes, i.e., 42 S haplotypes in B. rapa and 45 S haplotypes in B. oleracea. With these materials, we established a simple, efficient, and reliable dot-blot technique for S genotyping for 40 S haplotypes of B. rapa and and 33 of B. oleracea using allele-specific oligonucleotide probes and allele-specific primer pairs designed from sequences of each SP11 allele. In this method, DNA fragments amplified using multiplex primer pairs with digoxigenin-dUTP were hybridized with dot-blotted
allele-specific oligonucleotide probes with distinct signals. In addition, we developed a screening method for identification
of plants harboring a particular S haplotype using a labeled allele-specific oligonucleotide probe. This method is considered to be useful for purity testing
of F1 hybrid seeds. 相似文献
15.
A gene encoding aminolevulinate synthase (ALA-S) in yeast (Saccharomyces cerevisiae
YHem1) was introduced into the genome of tobacco (Nicoliana
tabacum) under the control of Arabidopsis thaliana HemA1 gene promoter (AtHemA1 P). All transgenic lines transcribed the YHem1 gene, especially under light condition. The capacity to synthesize ALA and therefore chlorophyll was increased in transformed
plants. Determination of gas exchange suggested that transgenic plants had significantly higher level of net photosynthetic
rate (P
n
), stomatal conductance (G
s
) and transpiration rate (T
r
), compared to the wild type (WT). Analysis with a modulated chlorophyll fluorometer demonstrated that the genetic transformation
also caused a significant increase in photochemical efficiency of PSII (F¢v /F¢m F^{\prime}_{v} /F^{\prime}_{m} ), actual photochemical efficiency (Ф
PSII
), photochemical quenching (qP), electron transfer rate (ETR) and the energy proportion in photochemistry (Pc), but decrease in proportion in heat dissipation (Hd). Chlorophyll-a fast fluorescence measurement and JIP-test indicated that photosynthetic performance index on cross section
basis (PI
CS
) and electron transport flux (ET
o
/CS) of transgenic tobacco were increased remarkably. And the probability that a trapped exciton can move a electron into the
electron transport chain beyond Q
A
− (Ψ
o
) and the density of active reaction centers (RC/CS) were also increased obviously in transgenic tobacco. These results imply that transgenic tobacco plants expressing YHem1 gene had higher photosynthetic capacity and energy conversion efficiency than the WT plants. 相似文献
16.
Nongporn Hutadilok-Towatana Wantana Reanmongkol Pharkphoom Panichayupakaranant 《Journal of applied phycology》2010,22(5):599-605
In this study, the methanol extract of Arthrospira (Spirulina) platensis was examined for acute and subchronic toxicities. The extract did not produce any sign of toxicity within 7 days after feeding
it at a single high dose of 6 g kg−1 body weight to female and male Swiss mice. For the subchronic toxicity test, the extract at doses of 6, 12, and 24 mg kg−1 body weight was orally administered to six male and six female Wistar rats daily for 12 weeks. Throughout the study period,
we did not observe any abnormalities on behavior, food and water intakes, and health status among the treated animals. The
hematology and clinical chemistry parameters of treated groups did not significantly differ from those of the controls in
both sexes. Postmortem examination of the test groups also showed no abnormalities in both gross and histological findings.
These results thus suggest that the methanol extract of A. platensis did not cause acute or subchronic toxicity in our experimental animals. 相似文献
17.
Mohsin Abbas Zaidi Gongyin Ye Hongwei Yao Taek H. You Evelin Loit Donald H. Dean Sheikh Riazuddin Illimar Altosaar 《Molecular biotechnology》2009,43(3):232-242
Nucleotide sequence encoding the truncated insecticidal Cry1Ca1 protein from Bacillus thuringiensis was extensively modified based on the codon usage of rice genes. The overall G + C contents of the synthetic cry1Ca1 coding sequence were raised to 65% with an additional bias of enriching for G and C ending codons as preferred by monocots.
The synthetic gene was introduced into the Chinese japonica variety, Xiushui 11, by Agrobacterium-mediated transformation. Transgenic rice plants harboring this gene were highly resistant to Chilo
suppressalis and Spodoptera litura larvae as revealed by insect bioassays. High levels of Cry1Ca1 protein were obtained in the leaves of transgenic rice, which
were effective in achieving 100% mortality of S. litura and C. suppressalis larvae. The levels of Cry1Ca1 expression in the leaves of these transgenic plants were up to 0.34% of the total soluble proteins.
The larvae of C. suppressalis and S.
litura could consume a maximum of 1.89 and 4.89 mm2 of transgenic leaf area whereas the consumption of non-transgenic leaves by these larvae was significantly higher; 58.33 and
61.22 mm2, respectively. Analysis of R1 transgenic plants indicated that the cry1Ca1 was inherited by the progeny plants and provided complete protection against C. suppressalis and S.
litura larvae. 相似文献
18.
Zhao BY Ardales E Brasset E Claflin LE Leach JE Hulbert SH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,110(1):71-79
The genetic mechanism underlying six palatability properties of cooked rice and three physico-chemical traits was dissected in 66 BC3F2 chromosome segment substitution lines (CSSLs), using a complete linkage map in three successive years. The CSSLs showed transgressive segregation for all traits studied. Significant correlation was detected among most palatability traits. A total of 25 QTLs for the nine traits were identified on nine chromosomes, and many QTLs affecting different quality traits were mapped in the same regions. Six QTLs—qLT-8 for luster, qTD-6 and qTD-8 for tenderness, qIVOE-6 and qIVOE-8 for integrated value of organoleptic evaluation, and qAC-8 for amylose content—were repeatedly detected across the 3 years. Phenotypic values were significantly different between the recurrent parent, cultivar Asominori, and the CSSLs harboring any of the six QTL alleles across the three environments, indicating that these six QTLs were non-environment-specific and could be used for marker-assisted selection in rice quality improvement. 相似文献
19.
Perugini LD Murphy JP Marshall D Brown-Guedira G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(3):417-425
Powdery mildew is an important foliar disease in wheat, especially in areas with a cool or maritime climate. A dominant powdery
mildew resistance gene transferred to the hexaploid germplasm line NC99BGTAG11 from T. timopheevii subsp. armeniacum was mapped distally on the long arm of chromosome 7A. Differential reactions were observed between the resistance gene in
NC99BGTAG11 and the alleles of the Pm1 locus that is also located on chromosome arm 7AL. Observed segregation in F2:3 lines from the cross NC99BGTAG11 × Axminster (Pm1a) demonstrate that germplasm line NC99BGTAG11 carries a novel powdery mildew resistance gene, which is now designated as Pm37. This new gene is highly effective against all powdery mildew isolates tested so far. Analyses of the population with molecular
markers indicate that Pm37 is located 16 cM proximal to the Pm1 complex. Simple sequence repeat (SSR) markers Xgwm332 and Xwmc790 were located 0.5 cM proximal and distal, respectively, to Pm37. In order to identify new markers in the region, wheat expressed sequence tags (ESTs) located in the distal 10% of 7AL that
were orthologous to sequences from chromosome 6 of rice were targeted. The two new EST-derived STS markers were located distal
to Pm37 and one marker was closely linked to the Pm1a region. These new markers can be used in marker-assisted selection schemes to develop wheat cultivars with pyramids of powdery
mildew resistance genes, including combinations of Pm37 in coupling linkage with alleles of the Pm1 locus. 相似文献
20.
Weijun Ye Shikai Hu Liwen Wu Changwei Ge Yongtao Cui Ping Chen Jing Xu Guojun Dong Longbiao Guo Qian Qian 《Plant Growth Regulation》2017,81(1):81-90
Chlorophyll (Chl) content is an important agronomic trait directly affecting the photosynthetic rate. Using a high-density genetic map of 132 recombinant inbred lines (RILs) derived from the cross between 93-11 and PA64s, we detected the quantitative trait loci (QTLs) for Chl content of the top three leaves under two nitrogen (N) conditions at two developmental stages. A total of 32 main-effect QTLs located on chromosomes 1, 4, 5, 6, 7, 8, and 12 were identified, and these QTLs individually accounted for 6.0–20.8?% of the total phenotypic variation. A major QTL qFCC7 L affecting the Chl content under low N condition was identified, and its positive allele came from PA64s. This QTL might be associated with the ability to tolerate low-N stress in rice. The chromosomal segment substitution line (CSSL) with the corresponding segment from PA64s had a higher SPAD value and photosynthetic rate than 93-11 and showed a lower specific leaf area (SLA). We performed a fine-mapping using a BC4F2 population via marker-assisted backcross and finally mapped this QTL to a 124.5 kb interval on the long arm of chromosome 7. Candidate gene analysis showed that there were sequence variations and expression differences in the predicted candidate gene between the two parents. These results suggest that the QTL qFCC7 L may be useful for breeding the rice varieties with higher photosynthetic rate and grain yield. 相似文献