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1.
To evaluate the species specificity of Cd accumulation and the relationship of Cd with other essential metals and metallothionein
(MT), the concentrations of Cd, Zn, Cu, and Fe in the liver and kidney and the MT concentrations in the soluble fractions
of the liver and kidney were determined in Cd-uncontaminated nonhuman primates (11 species, 26 individuals) kept in a zoo
and two wild-caught Japanese macaques. The compositions of metal-binding proteins in the soluble fractions were also investigated
by high-performance liquid chromatography (HPLC). The hepatic Cd concentration was 0.03–14.0 μg/g and the renal Cd concentration was 0.35–99.0 μg/g, both varying greatly and being higher in nonhuman primates, which were more closely related to man. The hepatic Zn concentration
was 24.0–176 μg/g and the renal Zn concentration was 13.5–138 μg/g, showing 7- to 10-fold differences, and a correlation (r=0.558, p<0.01) was found between renal Zn and renal Cd concentrations.
It was proved that in the liver, MT is more closely correlated with Zn (r=0.795, p<0.001) than with Cd (r=0.492, p<0.01) and that in the kidney MT is correlated with both Cd (r=0.784, p<0.001) and Zn (r=0.742, p<0.001). HPLC analysis of metals bound to MT-like protein in chimpanzees, de Brazza’s monkeys, and Bolivian squirrel monkeys
showed that more than 90% of Cd in both the liver and kidney, approx 40% of Zn in liver and 28–69% of Zn in kidney were bound
to MT-like protein. The higher percentage Zn was bound to high-molecular protein. 相似文献
2.
Zachara BA Koterska D Manitius J Sadowski L Dziedziczko A Salak A Wasowicz W 《Biological trace element research》2004,97(1):15-30
Patients with chronic renal failure (CRF) usually have a lower than healthy level of selenium (Se) in whole blood and plasma.
Plasma glutathione peroxidase (GSH-Px) is synthesized mostly in the kidney. In CRF patients, activity of this enzyme is significantly
reduced and its reduction increases with the progress of the disease. The aim of the study was to evaluate the effect of Se
supplementation to CRF patients at various stages of the disease on Se concentration in blood components and on plasma GSH-Px
activity.
The study group comprised 53 CRF patients at various stages of the disease supplemented with Se (200 μg/d for 3 mo as Se-enriched
yeast, containing about 70% l-selenomethionine [SeMet]). The control group consisted of 20 healthy subjects. The Se concentration in blood components was
measured spectrofluorometrically with 2,3-diaminonaphthalene as a complexing reagent. GSH-Px activity in red cell hemolysates
and plasma was assayed by the coupled method with tert-butyl hydroperoxide as a substrate.
The Se concentration in whole blood and plasma of CRF patients is significantly lower as compared with healthy subjects, but
similar at all stages of the disease. In the patients’ plasma, total protein and albumin levels are also significantly lower
than in healthy subjects. Plasma GSH-Px activity in patients is extremely low, and contrary to Se concentration, it decreases
linearly with the increasing stage of the illness. Se-supplied patients show an increased Se concentration in all blood components
and at all disease stages, whereas plasma GSH-Px activity is enhanced only at the incipient stage of the disease. Se supply
has no effect on plasma GSH-Px activity in uremic patients at the end stage of the disease. Total plasma protein and albumin
levels did not change after Se supplementation. Our data seem to show that in patients with CRF lower total protein and albumin
levels in plasma may be the chief cause of the low blood and plasma Se concentrations. GSH-Px activity decreases along with
the kidney impairment. At the end stage of the disease, Se supplementation in the form of Se-enriched yeast has no effect
on the increase in plasma GSH-Px activity. 相似文献
3.
Selenium and glycogen levels in diabetic patients 总被引:2,自引:0,他引:2
Selenium in serum and selenium and glycogen in erythrocytes were determined in diabetic patients divided into noninsulin-dependent
(n=50) and insulin-dependent (n=31) groups according to the etiopathogenesis of their diabetes. Selenium was determined by the method of atomic absorption
spectrometry. Serum level of selenium was statistically significantly different in patients with either noninsulin-dependent
(59.23±12.2 μg/L) or insulin-dependent (58.23±16.7 μg/L) diabetes mellitus as compared with the control group of 62 subjects
(64.2±11.5 μg/L; p<0.05). There was no statistically significant difference in the serum levels of selenium between the groups of patients with
noninsulin-dependent and insulin-dependent diabetes mellitus. The levels of erythrocyte glycogen were 2.0580±1.326, 2.0380±1.735,
and 2.0036±1.3537 μg/g Hb in the control group, noninsulin-dependent group, and insulin-dependent group, respectively, with
no statistically significant between-group difference. The decreased levels of selenium in serum and erythrocytes of diabetic
patients suggest the possible role of glutathione peroxidase activity. 相似文献
4.
The relationships among concentrations of copper and zinc, the oxidase activity of ceruloplasmin (Cp) in serum, and Cu,Zn-SOD
(superoxide dismutase) activity in erythrocytes were investigated in men with atherosclerosis obliterans (AO) and a control
group. The oxidase activity of Cp was measured with o-dianisidine dihydrochloride as a substrate, and Cu,Zn-SOD activity in erythrocytes by using the RANSOD kit. The lipid profile
and uric acid concentration were determined in AO and control groups.
The results showed higher copper and zinc concentrations in serum in the AO group (20.0±3.5 and 18.0±3.2 μmol/L, respectively)
in comparison with the control group (15.6±2.3 and 14.7±1.9 μmol/L). The Cp activity in serum was higher in the AO group (174.2±61.8
U/L) than in the control group (93.7±33.9 U/L), and a significant difference was found in the activity of Cu,Zn-SOD in erythrocytes
(2389±1396 and 1245±365 U/g Hb, respectively) between both groups.
The activity of Cu,Zn-SOD was positively correlated with copper in the control group (r=0.73), but not in AO, and negatively with uric acid concentration (r=−0.63) in the AO group. The oxidase activity of Cp was correlated with copper, but not zinc, in AO and control groups (r≥0.65). Negative correlation coefficients were calculated for uric acid and copper and zinc concentrations in the AO group
(−r≥0.61).
Increased copper concentrations and oxidase activity of Cp in serum in AO and the activity of Cu,Zn-SOD in erythrocytes could
result from atherosclerotic disease, accompanied by chronic ischemia of a lower limb. These results suggest also that relationship
between copper concentration and Cu,Zn-SOD activity in erythrocytes found in the serum of healthy subjects may be disturbed
in pathologic conditions. 相似文献
5.
Gorelik O Efrati S Berman S Almoznino-Sarafian D Alon I Shteinshnaider M Cohen N 《Biological trace element research》2007,120(1-3):102-109
Deficiency of intracellular magnesium (icMg) may coexist with normal serum Mg levels. Little is known about clinical and pharmacological
factors affecting icMg in normomagnesemic patients with diabetes mellitus (DM). Moreover, no information exists regarding
the icMg state in diabetic patients after acute illness and before hospital discharge. We have evaluated the effect of antihyperglycemic
medications and other relevant clinical variables on icMg in 119 such patients. Total icMg was measured in peripheral blood
mononuclear cells. Twenty healthy volunteers served as controls. IcMg content (μg/mg cell protein) was lower in DM compared
to controls (1.74 ± 0.44 vs 2.4 ± 0.39, p < 0.001). It was also significantly lower in patients treated with insulin (1.57 ± 0.31 vs 1.8 ± 0.46, p = 0.01), while metformin treatment was associated with higher icMg (1.86 ± 0.49 vs 1.63 ± 0.35, p = 0.003). After adjustment for age, gender, and concomitant use of other hypoglycemic drugs, only treatment with metformin
was independently associated with increased icMg (p = 0.03). No statistically significant association or correlation was found between icMg content and age, causes of hospitalization,
comorbid conditions, treatment with other drugs, concentrations of HbA1c, serum glucose, Mg, or creatinine. In conclusion, icMg is depleted in normomagnesemic DM patients. Insulin treatment is associated
with worsening of icMg status, while metformin treatment may confer protective effect. 相似文献
6.
Zinc (Zn) and copper (Cu) concentrations in hair and urine of patients diagnosed and hospitalized for myocardial infarction
(MI patients) and in their descendants (MI descendants) were estimated and compared with their age-matched healthy volunteers
with no family history of MI (control group and control descendants). The data revealed approximately twofold higher Zn and
twofold lower Cu in the urine of the patients; Zn was lower and Cu was higher in the urine of MI descendants than those of
the patients (p<0.001), but Zn in hair and urine was higher and Cu in hair was lower in MI descendants compared with their control counterparts
(p<0.001).
The data suggested that there was a consistent rise in Zn and fall in Cu reserves in the genetically predisposed subjects
(MI descendants) prior to the manifestation of clinical symptoms. Based on this, the data were subjected to logistic regression
and a model was obtained to predict the susceptibility to MI (LR-MI), having impact factors values as follows: constant (C),
−3.342; impact factor of body mass index, −0.776; impact factor of hair Zn, −2.449; impact factor of urine Zn, +3.441; impact
factor of hair Cu, −15.077; impact factor of urine Cu, −24.153.
For the equation Y=e
x
(1+e
x
), the value of x was obtained as follows: −3.342+[BMI (kg/m2) (−0.776)]+[Hair Zn (μmol/g) (−2.449)]+[Urine Zn (μmol/L) (3.441)]+[Hair Cu (μmol/g) (−15.077)]+[Urine Cu (μmol/L) (−24.153)].
On substituting the values of BMI, hair Zn, urine Zn, hair Cu, and urine Cu in x, the response variable Y as zero for healthy controls and 0.99 or 99.9% susceptibility in MI patients were obtained. In between these two extremes,
the response variable ranged between 0 and 0.99 or 99.9% susceptibility to MI in their descendants. It is envisaged that the
MI patients have an operational component of a genetic disorder of ionic imbalance at a young age that can be exploited in
making a prediction of susceptibility to heart stroke in individuals much before its onset and diagnosis in asymptomatic patients,
particularly in genetic and epidemiological studies of MI. 相似文献
7.
The estimation of the selenium status during pregnancy is of great importance because of the significance of selenium for
fetus growth and antioxidant protection of neonates. This problem is especially urgent for Russia and its neighbors because
very little data are available and because data on soil selenium predict low intake levels of selenium. A large epidemiological
investigation made in various areas of the former USSR allowed us to obtain the first information concerning the subject.
Serum samples were obtained during 1990–1998 from 556 female blood donors aged 20–53 yr and 722 pregnant women (18–33 yr)
during different times of gestation. The mean serum selenium concentration of nonpregnant women varied from 0.87 μmol/L (Slavutich,
Ukraine) to 1.74 μmol/L (Ioshkar-Ola, Mary-El) and that of women at delivery from 0.66 μmol/L (Zaporozie, Ukraine) to 1.34
μmol/L (Sakhalin, Russia). Compilation of literature and present data on serum selenium showed the following relationships:
nonpregnant women versus women at delivery, y=x -0.25, r=0.94; women at delivery versus umbilical serum, log y=log x -0.2, r=0.97. The two relationships were used to predict serum selenium values for pregnant women taking into account the progressive
serum selenium decrease during advancing pregnancy. In almost half of the towns (i.e. 22–50%), pregnant women were considered
to have relative selenium deficiency. 相似文献
8.
Van Biervliet S Van Biervliet JP Bernard D Matthys M Vercaemst R Blaton V 《Biological trace element research》2001,79(2):115-120
Previous studies showed low selenium (Se) concentrations in Belgian children. Serum α-tocopherol, retinol, total cholesterol, high-density lipoprotein and low-density lipoprotein cholesterol, selenium (Se),
and thiobarbituric acid-reactive substances were examined. In order to obtain further information on the Se status in Belgian
children, Se, α-tocopherol, retinol, and lipid concentrations were examined and signs of peroxidative lipid damage were evaluated in a subgroup.
The study was performed in 524 children (0–14 yr old) during vaccination campaigns. Three age groups were analyzed: 0–1, 1–4,
and 4–14 yr. In 87 of them, where sufficient amounts of serum were available, analysis of thiobarbituric acid-reactive substances
was done.
Infants have high serum α-tocopherol concentrations: (23.2 μmol/L [median and interquartile range: 18.6–30.2]) and low Se concentrations (0.37 mol/L
[0.27–0.47]). Se concentrations rise significantly during the first 4 yr (p < 0.0001) (Mann-Whitney U-test, tied p-values): 0.70 μmol/L (0.59–0.82); in the 4–14 yr olds, it was 0.75 μmol/L (0.67–0.86). These values remain low compared to
results coming from other parts of the world. α-Tocopherol concentrations decrease significantly after infancy (p < 0.0001). The ratio α-tocopherol/total cholesterol is higher in infants. This is induced by the high vitamin E content of infant formulas. Signs
of serum lipid peroxidation could not be detected by analysis of serum malondialdehyde concentrations.
High α-tocopherol concentrations, as those observed in infant serum lipids, could be one of the protective mechanisms from the peroxidative
lipid damages, sometimes observed in a low-Se status. 相似文献
9.
Brtková A Magálová T Béderová A Babinská K Barteková S 《Biological trace element research》1999,67(1):49-54
Blood serum selenium levels were measured in 891 healthy children and adolescents (aged 11–18 yr, 450 girls and 441 boys)
residing in both rural and urban areas from eight regions of Slovakia. Subjects were divided into four age groups (11–12 y,
13–14 y, 15–16 y, and 17–18 y). Serum selenium concentration was determined by the electrothermal atomic absorption spectrometric
method. The mean (±SD) serum selenium concentrations were 0.750 ±0.255 μmol/L in girls and 0.773 ±0.235 μmol/L in boys. A
large proportion of the individuals (25.7% in girls, 18.1% in boys) exhibited serum selenium levels under 0.57 μmol/L (45
μg/L). An increasing trend of the serum selenium values with age has been observed in both boys (p < 0.01) and girls (p < 0.05). Boys had higher serum selenium levels in the all age groups but the differences were not statistically significant. 相似文献
10.
The concentration of zinc, copper, selenium, albumin, and ceruloplasmin in blood plasma and the activity of superoxide dismutase
and glutathione peroxidase in erythrocytes were determined in a set of patients with idiopathic scoliosis (n=51). A significant decrease of selenium concentration (0.50±0.16 μmol/L) was found when compared with a control group (0.69±0.07
μmol/L) (p<0.01). The same levels of significance were found out for selenium levels corrected for albumin content. In a group of patients
with a curvature over 45° indicated for a surgical correction, the average plasma concentrations of selenium were significantly
lower (p<0.05) in comparison with a group of patients with a curvature below 45° treated conservatively.
The GSH-Px activity in erythrocytes was the same in both sets. In comparison with the controls, no significant differences
were revealed in all of the other parameters. The detection of the decreased blood plasma concentration of selenium has suggested
possible disturbance of well-proportioned distribution and of general optimal availability of selenium in the organism of
patients with idiopathic scoliosis with likely effects on the process of synthesis and maturation of collagen affecting the
axial skeleton stability. 相似文献
11.
Bekheirnia MR Shamshirsaz AA Kamgar M Bouzari N Erfanzadeh G Pourzahedgilani N Tabatabaie SM Abdollah Shamshirsaz A Kimiagar M Ezzati F Larijani B 《Biological trace element research》2004,97(3):215-224
Trace elements have been considered to play critical roles in bone metabolism. This study aims at determining the serum zinc
profile and its association with bone mineral density (BMD) abnormalities in thalassemic patients. In 131 transfusion-dependent
β-thalassemic patients, aged 10–20 yr, serum levels of zinc were measured by flame atomic absorption spectrophotometry (F-AAS).
BMD values at the lumbar (L1–L4) and femoral neck were determined by dual X-ray absorptiometry (DXA). Dietary zinc intake
and daily consumption of calcium were evaluated by a food-frequency questionnaire. Low serum zinc was found in 84.8% (in 44.7%
severely low). Below −2 BMD Z-scores were observed in 68.7% and 17.6% of the patients at the lumbar and femoral regions, respectively.
Female patients with severe zinc deficiency had lower lumbar BMD Z-scores in comparison to the other females (−3.26 vs −2.54).
Serum zinc in females with femoral BMD Z-scores <−2 was significantly lower by 16.4 μg/dL than other females. Our study suggests
that serum levels of zinc can be lowered in the thalassemic patients and partly affect the BMD. 相似文献
12.
Summary 1. The epsilon 4 allele of the apolipoprotein E gene increases the risk of late onset familial and sporadic Alzheimer disease. Relation of epsilon 4 allele of the apolipoprotein E gene to various types of dementia and the onset of dementia were analyzed in the present study.2. The study comprised 139 patients (50 men and 89 women) with dementia, mean age 73.61 years (range 47–98). The diagnosis of dementia was made according to Diagnostic and Statistical Manual of Mental Disorders, and subtypes diagnoses were made according to NINCDS-ADRDA and NINDS-AIREN criteria. Minimental State Examination (MMSE) was used for the screening of dementia. Apolipoprotein E polymorphism was determined by the PCR-RFLP technique-polymerase chain reaction and subsequent digestion with specific restriction endonuclease. For statistical analyses chi-square test and the crude Gart′s odds ratio (OR) and 95% confidence intervals (CI) were used.3. From 139 dementia patients (MMSE ≤24 points) in 61 (45%) Alzheimer disease (AD) was present, in 44 patients (31%) vascular dementia (VD), and in 34 (24%) mixed dementia (MD) were revealed. In comparison with control group the presence of at least one ApoE-ɛ4 allele was significantly higher only in the group with AD (p < 0.001), (OR=2.76; 95%: 1.42–5.36). The frequency of ɛ4 allele carriers was significantly overrepresented in AD group compared with VD (χ2=5.94; p=0.0148). Differences between AD and MD or VD and MD were not confirmed. 相似文献
13.
Stability of transgene expression, field performance and recombination breeding of transformed barley lines 总被引:8,自引:0,他引:8
H. Horvath L. G. Jensen O. T. Wong E. Kohl S. E. Ullrich J. Cochran C. G. Kannangara D. von Wettstein 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(1):1-11
Stable inheritance of the transgene, consistent expression and competitive agronomic properties of transgenic crops are important
parameters for successful use of the latter. These properties have been analyzed with 18 homozygous transgenic barley lines
of the cultivar Golden Promise. The lines originated from three independent primary transformants obtained by the biolistic
method with three plasmids containing respectively, the bar gene, the uidA gene and the gene for a protein-engineered heat-stable (1,3–1,4)-β-glucanase. Three production levels of recombinant β-glucanase
were identified in homozygous transgenic T3 plants, and these remained constant over a 3-year period. In micro-malting experiments, the heat-stable enzyme reached levels
of up to 1.4 μg·mg−1 protein and survived kiln drying at levels of 70–100%. In the field trials of 1997 and 1998 the transgenic lines had a reduced
1000-grain weight as well as variable yield depressions compared to the Golden Promise progenitor. In 1999 large-scale propagations
of the lines with the highest recombinant enzyme synthesis during germination and of Golden Promise were studied at three
different locations. In an irrigated field transgenic lines yielded approximately 6 t·ha−1 and Golden Promise 7.7 t·ha−1. Cross-breeding was carried out to transfer the transgene into a more suitable genetic background. Crosses of the semi-dwarf
ari-e mutant Golden Promise gave rise to the four morphological phenotypes nutans, high erect, erect, and ari-e. Two improvements were achieved: (1) F3 lines homozygous for the expression of heat-stable (1,3−1,4)-β-glucanase were found among lines that were homozygous for
each of the four morphological phenotypes; (2) improved 1000-grain weights and yields with respect to those of the original
transformants were observed in some F4 lines homozygous for the morphological phenotypes and for the transgene. In the case of a homozygous nutans line, the transgenic
plants had a higher 1000-grain weight than those lacking the transgene. Like mutants providing useful output traits, transgenic
plants will often have to be improved by relocating the gene into more suitable genotypes.
Received: 6 March 2000 / Accepted: 14 April 2000 相似文献
14.
Summary. The levels of polyamines (putrescine, spermidine and spermine) and polyamine oxidase in plasma of patients with chronic renal
failure were determined. The level of putrescine was increased but the level of spermine was decreased in the plasma of these
patients. The patients also had increased plasma polyamine oxidase activity leading to increased degradation of spermine.
As acrolein was a major toxic compound produced from spermine by polyamine oxidase, the levels of free and protein-conjugated
acrolein in plasma were also measured. Acrolein levels were enhanced in plasma of patients with chronic renal failure. The
accumulated acrolein found as protein conjugates was equivalent to 170 μM, which was about 5-fold higher than in plasma of
normal subjects. It was found that acrolein is mainly produced by spermine oxidase in plasma. An increase in putrescine, spermine
oxidase and acrolein in plasma was observed in all cases such as diabetic nephropathy, chronic glomerulonephritis and nephrosclerosis.
After patients with chronic renal failure had undergone hemodialysis, their levels of plasma polyamines, spermine oxidase
and acrolein returned towards normal. It is likely that acrolein produced from spermine accumulates in the blood due to decreased
excretion into urine and may function as a uremic “toxin”. 相似文献
15.
Herbert Oberlander 《In vitro cellular & developmental biology. Plant》1976,12(3):225-235
Summary This paper reviews the effects of insect hormones on lepidopteran imaginal discs cultured in vitro.β-ecdysone stimulated both evagination and cuticle deposition of wing discs ofPlodia interpunctella (Hübner). However, evagination required a shorter exposure to ecdysone than did cuticle deposition. Cuticle deposition was
obtained under the following conditions: (a) a 24-hr pulse ofβ-ecdysone (0.5–5.0μg/ml); (b) continuous treatment with 0.2μg/mlβ-ecdysone; or (c) continuous treatment with 0.5 to 50.0μg/mlβ-ecdysone in medium conditioned with larval fat body.
Investigations of some biochemical effects of ecdysone showed that RNA and protein synthesis was required for evagination
and cuticle deposition. In particular, studies with actinomycin D and cycloheximide (at nontoxic levels) showed that RNA and
protein synthesis during the ecdysone-dependent period was essential for subsequent development. These findings support the
hypothesis that stimulation of macromolecular synthesis is fundamental to the action of ecdysone on imaginal discs.
The influence of beta-ecdysone on chitin synthesis was also examined.β-ecdysone stimulated uptake and incorporation of tritiated-glucosamine by culturedP. interpunctella wing discs. Addition of hexosamines to the culture medium had no influence on ecdysone-induced cuticle deposition, but inhibition
of glucose-uptake by cytochalasin B prevented the formation of cuticle. The action of ecdysone on particular enzymes in the
chitin pathway remains to be elucidated.
Presented in the formal symposium on Information Transfer in Eukaryotic Cells, at the 26th Annual Meeting of the Tissue Culture
Association, Montreal, Quebec, June 2–5, 1975. 相似文献
16.
Serwin AB Waşowicz W Gromadzińska J Chodynicka B 《Biological trace element research》2002,89(2):127-137
The aim of the study was to examine the influence of alcohol consumption on the severity of psoriasis and selenium (Se) concentration
and Se-dependent gluathione peroxidase activity in plasma (pl-GSH-Px) and in erythrocytes (RBC-GSH-Px) in psoriatic patients.
Thirty-five in-patients with psoriasis lasting <10 mo and 42 with psoriasis lasting >3 yr constituted groups 1 and 2, respectively.
The severity of psoriasis was assessed using the PASI scoring system and the consumption of alcohol, using a structured questionnaire.
The Se concentration was 47.11±11.61 μg/L in group 1 and 38.69±13.22 μg/L in group 2 (p<0.05), the pl-GSH-Px was 0.15±0.04 U/mL and 0.14±0.04 U/mL (p>0.05), and the RBC-GSH-Px was 13.97±4.27 U/g Hb and 13.16±3.85 U/g Hb (p>0.05), respectively. In excessive drinkers (<10% of patients, all males), the Se concentration was 32.84±10.88 μg/L, the
pl-GSH-Px was 0.15±0.03 U/mL, and the RBC-GSH-Px was 11.64±3.32 U/g Hb. A low RBC-GSH-Px correlated to the consumption of
high-grade alcoholic beverages (R=−0.45, p<0.05) and to the PASI value (R=−0.37, p<0.05) in group 2. Depressed Se concentration and Se-dependent GSH-Px can be related to the severity and a duration of psoriasis.
The excessive consumption of alcohol is associated with severity of the disease and with low activity of GSH-Px in erythrocytes
in patients with long-lasting psoriasis. 相似文献
17.
Baolan Hao Hengwei Zhang Yu-ping Ye Xinjian Hao Yan Wang 《Asia-Pacific Journal of Blood Types and Genes》2018,2(4):249-253
Many cancer patients are known to present in a hypercoagulable state, meaning an increased risk of thrombosis. To investigate hypercoagulable state in breast cancer (BC) patients, their coagulation status was compared with a benign disease group (control). The BC patients were divided into earlier stage (stage I and stage Ⅱ ) and later stage (stage Ⅲ and stage Ⅳ ). Thrombelastography (TEG) and other traditional coagulation tests were performed. The results showed that prothrombin time (PT) was significantly shortened and the levels of D-dimer, fibrinogen (Fib) and platelets (PLT) were significantly increased in the traditional BC group test (P< 0.05). According to TEG detection, the average level of blood clot formation time (K) was significantly lower, while the Angle, MA and CI were significantly higher in the BC group than those in benign disease group (P< 0.05). There were 5 cases of lower extremity venous thrombosis in the breast cancer patients, coinciding with hypercoagulable state. The results showed that the BC patients had an increased hypercoagulable state, with hypercoagulability becoming more obvious in advanced stages. This study suggests that BC patients have an increased tendency for clot formation, and TEG monitoring could be a useful tool to predict the risk of thrombosis for clinical prevention and treatment. 相似文献
18.
Effects of exogenous methyl jasmonate in elicited anthocyanin-producing cell cultures of ohelo (Vaccinium phalae) 总被引:1,自引:0,他引:1
Yimin Fang M. A. L. Smith M. -F. Pépin 《In vitro cellular & developmental biology. Plant》1999,35(1):106-113
Summary Elicitation of anthocyanin-producing cells of ohelo (Vaccinium pahalae) by both biotic (purified β-glucan and chitosan) and abiotic [sodium ferric ethylenediamine di-(o-hydroxyphenylacetate) FeEDDHA, and CuSO4] elicitors resulted in significant enhancement of anthocyanin accumulation. Anthocyanin production increased up to 1.8 and
1.5-fold over the control in the presence of abiotic elicitors (90 μM FeEDDHA and 20 μM CuSO4, respectively), and increased 1.9 and 1.6-fold in the presence of biotic elicitors (10 mg L−1 β-glucan and 100 mg L−1 chitosan). Maximum anthocyanin production with the two most effective elicitors was achieved when cultures were treated on
Day 3 (β-glucan) or Day 0 (FeEDDHA) after the initiation of fresh cell cultures. A concentration-dependent response was exhibited
by cultures treated with exogenous methyl jasmonate (MJ). The addition of 0.5 μM MJ alone provoked a 2–3-fold increase in anthocyanin production over that of the control; however, no additive effect on
anthocyanin production was observed in any treatments which combined MJ and β-glucan or FeEDDHA. Conditioning of the cells
with a preculture in either MJ, β-glucan, or FeEDDHA similarly did not enhance anthocyanin production. Inoculation of cultures
elicited by MJ or β-glucan with ibuprofen, a reported inhibitor of jasmonate biosynthesis, dramatically stimulated, rather
than inhibited, anthocyanin production, resulting in levels of accumulation beyond any of the tested elicitor combinations.
Hypotheses for the observed influence of ibuprofen in this system are discussed. 相似文献
19.
James Springate Kenneth Chan Hong Lu Sherry Davies Mary Taub 《In vitro cellular & developmental biology. Animal》1999,35(6):314-317
Summary Renal injury is a common side effect of the chemotherapeutic agent ifosamide. Current evidence suggests that the ifosfamide
metabolite chloroacetaldehyde may contribute to this nephrotoxicity. The present study examined the effects of ifosfamide
and chloroacetaldehyde on rabbit proximal renal tubule cells in primary culture. The ability of the uroprotectant medication
sodium 2-mercaptoethanesulfonate (mesna) to prevent chloroacetaldehyde-induced renal cell injury was also assessed. Chloroacetaldehyde
(12.5–150 μM) produced dose-dependent declines in neutral red dye uptake, ATP levels, glutathione content, and cell growth. Coadministration
of mesna prevented chloroacetaldehyde toxicity while pretreatment of cells with the glutathione-depleting agent buthionine
sulfoximine enhanced the toxicity of chloroacetaldehyde. Ifosfamide (1000–10 000 μM) toxicity was detected only at concentrations of 4000 μM or greater. Analysis of media collected from ifosfamide-treated cell cultures revealed the presence of several ifosfamide
metabolites, demonstrating that renal proximal tubule cells are capable of biotransforming this chemotherapeutic agent. This
primary renal cell culture system should prove useful in studying the cause and prevention of ifosfamide nephrotoxicity. 相似文献
20.
E. E. Grintsevich V. V. Senchuk A. V. Puchkaev O. I. Shadyro D. I. Metelitsa 《Russian Journal of Bioorganic Chemistry》2000,26(8):741-751
A comparative kinetic study on the poly(gallic acid disulfide) (poly(DSGA)) inhibition of the iodide ion oxidation and on
the 2-hydroxy-3,5-di-tert-butyl-N-phenylaniline (butaminophene) inhibition of 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation involving human thyroid peroxidase
(hTPO) and horseradish peroxidase (HRP) was performed. The inhibition processes were characterized with the inhibition constantsK
i and stoichiometric inhibition coefficientsf, indicating the number of radical particles perishing on one inhibitor molecule. In the case of poly(DSGA), theK
i values for the I− oxidation were 0.60 and 0.04 μM, and the coefficientsf were 13.6 and 16.5 for hTPO and HRP, respectively, which evidences the regeneration and high effectiveness of the polymeric
inhibitor. In the case of butaminophene, theK
i values for TMB oxidation were 38 and 46 μM for hTPO and HRP, respectively. The coefficientsf were 1.33 and 1.47, respectively, to reveal that butaminophene does not regenerate. The inhibition mechanisms for I− and TMB oxidation involving the two peroxidases are discussed. 相似文献