地衣芽胞杆菌活菌制剂的临床应用进展

整肠生是应用二十多年的地衣芽胞杆菌活菌制剂,已有大量的研究报道其临床有效性和安全性。地衣芽胞杆菌大量分泌种类丰富的消化酶,通过抑制肠道有害菌生长和促进有益菌增殖调节肠道菌群,并产生杆菌肽、地衣素和乙酸等生物活性物质发挥益生作用。本文总结了益生菌的益生特点,并重点分析了芽胞杆菌的益生特点,归纳了整肠生的作用机制与临床研究现状,揭示了其在肝脏疾病、溃疡性结肠炎、肠易激综合征、幽门螺旋杆菌感染以及病毒感染等疾病中的治疗作用,并对整肠生未来的临床应用方向进行了展望。     

几种益生元制剂对肠道菌群作用效果的研究

目的探讨几种益生元制剂对肠道菌群作用效果。方法通过体外实验和体内试验。结果低聚果糖、水苏糖、低聚木糖、低聚异麦芽糖均能促进双歧杆菌和乳杆菌的增殖,并能够酸化肠道的pH;能够显著提高肠道的B／E值（肠道内双歧杆菌和肠杆菌数量log值的比值）,增加肠道中有益菌的比例,有益于稳定肠道的微生态平衡。结论这几种益生元制剂对肠道菌群有较好的调节作用。     

微生态制剂的作用机制及其在动物生产中的应用

近年来,微生态制剂的开发与应用受到社会各界的普遍关注。微生态制剂是一类优质饲料添加剂,具有安全性高、无致病性、毒副作用小和生物功能齐全的特点,包括益生菌、益生元和合生元三类物质,其在维持肠道菌群平衡、促进免疫系统发育及提高机体抗氧化性能等方面具有显著作用,可改善生产性能和健康状况,有利于动物生产优质产品。详细论述了微生态制剂的种类和益生菌、益生元和合生元的作用机制以及国内外关于微生态制剂在动物生产中的应用现状和对喀斯特地区健康养殖的应用潜力,从而为微生态制剂的研究方向及生产应用提供参考。     

乐腹康腊样芽胞杆菌在正常人体肠道定植及作用

腊样芽胞杆菌活菌制剂对人体肠道菌群的调节作用、能够预防和治疗胃肠道疾患已被许多实验和临床效果证实。为进一步观察其在人体肠道的生长、繁殖、停留时间和作用过程,我们观察了健康人体在口服乐腹康胶囊前后,其粪便中腊样芽胞杆菌的增殖情况,结果如下：１材料与方法...     

地衣芽胞杆菌在消化系统疾病中的应用

地衣芽胞杆菌在土壤等环境中普遍存在,体外研究显示该菌株的代谢产物可对致病菌的生长产生抑制作用。地衣芽胞杆菌活菌制剂进入肠道后可产生多种抗菌物质,并可通过生物夺氧作用改善肠道内环境,具有调节肠道微生态,改善消化道屏障功能,减轻炎症反应,增强免疫功能等作用,在肠道菌群失调相关疾病中应用前景广阔。地衣芽胞杆菌活菌制剂已广泛应用于腹泻疾病的治疗,并在炎症性肠病、某些肝病及幽门螺杆菌根除等治疗的研究中显示出其在改善肠道内环境,促进病情缓解的疗效。本文将对地衣芽胞杆菌活菌制剂及其消化系统疾病中的应用进行简要的叙述,并对该益生菌的研究方向做初步的探讨。     

动物肠道菌群的研究进展

肠道微生物是由存在于肠道部位的细菌、古菌、真菌、原生动物以及病毒所组成的群体。人们通过对细菌16SrRNA序列进行测序与分析,对动物及其在特定条件下的肠道菌群进行了相关方面的研究并取得了很大的进展。此外,由微生态原理发展而成的益生菌、益生元和合生元等微生态制剂,可维持机体肠道微生物的平衡、提高肠道屏障功能、增强机体免疫力。因此越来越多的微生态制剂应用于畜牧业、水产业中。论文综述了小鼠、犬、猪这三种动物的肠道菌群及其应用的研究进展,旨在为进一步深入了解动物肠道菌群的结构、功能以及促进微生态制剂的应用提供参考。     

地衣芽胞杆菌活菌制剂(整肠生)治疗肠道菌群失调56例报告

1 对象与方法 1.1 整肠生药性为地衣芽胞杆菌微生态制剂,通过以菌制菌的方法,利用地衣芽胞杆菌具有拮抗肠道致病菌,进入肠道后并且有生物夺氧,造成肠道低氧,促进生理性有益菌增长,以调节人体肠道的微生态环境,进而发挥生物学活力,起防病治病作用.     

地衣芽胞杆菌活菌制剂（整肠生）治疗肠道茵群失调56例报告

1对象与方法 1．1整肠生药性为地衣芽胞杆菌微生态制剂,通过以菌制菌的方法,利用地衣芽胞杆菌具有拈抗肠道致病菌,进入肠道后并且有生物夺氧,造成肠道低氧,促进生理性有益菌增长,以调节人体肠道的微生态环境,进而发挥生物学活力,起防病治病作用。     

益生菌调控胃肠道菌群改善肉品质的研究进展

胃肠道菌群对动物健康和生产性能起到重要作用。将益生菌应用于畜禽养殖可调节肠道微生态平衡,调控脂肪代谢,提高饲料利用率以及促进风味物质形成等多种途径提高畜禽肉的品质。本文综述了不同益生菌调节胃肠道微生态的机理,并通过改善肠道菌群结构来改善畜禽肉的品质,为进一步研究开发以胃肠道菌群为靶点的益生菌饲料提供参考。     

蕈状芽胞杆菌的特性及在辐射防护中的应用

作者在１９９４年从土壤中分离到一株蕈状芽胞杆菌,对该菌朱的生物学特性进行了鉴定。结果表明,该菌株无毒,无副作用。可以反它作为一种微生态制剂,在辐射防护中进行应用。通过研究细菌防护液的辐射防护效果,结果显示,对照射小鼠３０天活存率提高４５５,并能保护小鼠肠道菌群中的双歧杆菌和乳杆菌。     

乳杆菌胞外多糖合成基因簇及构效关系

肠道菌群稳态对维护人体的健康具有至关重要的作用。作为肠道天然微生物群“守卫兵”,益生菌参与改善体内微生态平衡。乳杆菌(Lactobacillus)是一种肠道益生菌的代表,其合成的胞外多糖(exopolysaccharide, EPS)既可通过增益肠道内其他益生菌的生长来优化肠道微生态,还具有抗肿瘤、抗氧化、降胆固醇、降血压和增强机体免疫力等益生功能。本文对近年来乳杆菌胞外多糖的遗传、生物学活性、构效关系等方面的研究进展,进行综述和展望。     

Fate and dissemination of Bacillus subtilis spores in a murine model

Bacterial spores are being consumed as probiotics, although little is known about their efficacy or mode of action. As a first step in characterizing spore probiotics, we have studied the persistence and dissemination of Bacillus subtilis spores given orally to mice. Our results have shown that spores do not appear to disseminate across the mucosal surfaces. However, we found that the number of spores excreted in the feces of mice was, in some experiments, larger than the original inoculum. This was an intriguing result and might be explained by germination of a proportion of the spore inoculum in the intestinal tract, followed by limited rounds of cell growth and then sporulation again. This result raises the interesting question of whether it is the spore or the germinated spore that contributes to the probiotic effect of bacterial spores.     

Fate and Dissemination of Bacillus subtilis Spores in a Murine Model

Bacterial spores are being consumed as probiotics, although little is known about their efficacy or mode of action. As a first step in characterizing spore probiotics, we have studied the persistence and dissemination of Bacillus subtilis spores given orally to mice. Our results have shown that spores do not appear to disseminate across the mucosal surfaces. However, we found that the number of spores excreted in the feces of mice was, in some experiments, larger than the original inoculum. This was an intriguing result and might be explained by germination of a proportion of the spore inoculum in the intestinal tract, followed by limited rounds of cell growth and then sporulation again. This result raises the interesting question of whether it is the spore or the germinated spore that contributes to the probiotic effect of bacterial spores.     

微生态系统和微生态制剂的研究进展

阐述了微生态系统和微生态制剂的定义;概述了口腔、咽部、及胃肠道微生态系统的研究现状和发展方向;概述了胃肠道内微生物群的种类、相互作用机制,以及针对胃肠道微生态系统应运而生的微生态制剂的作用原理和种类。并对未来微生态系统的发展和应用领域做出了展望。     

多器官功能不全综合征时肠道细菌微生态学改变的实验研究

为研究多器官功能不全综合征（ＭＯＤＳ）时肠道细菌微生态的变化以及Ｇ＾－杆菌消长与肠道和血液中内毒素水平的关系,本实验选用ＳＤ大鼠,应用无菌Ｚｙｍｏｓａｎ腹腔注射制备大鼠ＭＯＤＳ模型,并对ＭＯＤＳ大鼠肠道菌群进行定量分析,对门静脉和外周静脉血中的内毒以及肠道游离内毒素含量进行定量测定。结果发现,ＭＯＤＳ状态下肠道细菌微生态发生明显变化,表现为肠杆菌和肠球菌等肠道内需氧菌的数量明显增多,双歧杆菌和乳酸     

Gastrointestinal microecology: a crucial and potential target in acute pancreatitis

In the early stage of acute pancreatitis (AP), abundant cytokines induced by local pancreatic inflammation enter the bloodstream, further cause systemic inflammatory response syndrome (SIRS) by “trigger effect”, which eventually leads to multiple organ dysfunction syndrome (MODS). During SIRS and MODS, the intestinal barrier function was seriously damaged accompanied by the occurrence of gut-derived infection which forms a “second hit summit” by inflammatory overabundance. Gastrointestinal microecology, namely the biologic barrier, could be transformed into a pathogenic state, which is called microflora dysbiosis when interfered by the inflammatory stress during AP. More and more evidences indicate that gastrointestinal microflora dysbiosis plays a key role in “the second hit” induced by AP gut-derived infection. Therefore, the maintenance of gastrointestinal microecology balance is likely to provide an effective method in modulating systemic infection of AP. This article reviewed the progress of gastrointestinal microecology in AP to provide a reference for deeply understanding the pathogenic mechanisms of AP and identifying new therapeutic targets.     

The use of bacterial spore formers as probiotics

The field of probiosis has emerged as a new science with applications in farming and aqaculture as alternatives to antibiotics as well as prophylactics in humans. Probiotics are being developed commercially for both human use, primarily as novel foods or dietary supplements, and in animal feeds for the prevention of gastrointestinal infections, with extensive use in the poultry and aquaculture industries. The impending ban of antibiotics in animal feed, the current concern over the spread of antibiotic resistance genes, the failure to identify new antibiotics and the inherent problems with developing new vaccines make a compelling case for developing alternative prophylactics. Among the large number of probiotic products in use today are bacterial spore formers, mostly of the genus Bacillus. Used primarily in their spore form, these products have been shown to prevent gastrointestinal disorders and the diversity of species used and their applications are astonishing. Understanding the nature of this probiotic effect is complicated, not only because of the complexities of understanding the microbial interactions that occur within the gastrointestinal tract (GIT), but also because Bacillus species are considered allochthonous microorganisms. This review summarizes the commercial applications of Bacillus probiotics. A case will be made that many Bacillus species should not be considered allochthonous microorganisms but, instead, ones that have a bimodal life cycle of growth and sporulation in the environment as well as within the GIT. Specific mechanisms for how Bacillus species can inhibit gastrointestinal infections will be covered, including immunomodulation and the synthesis of antimicrobials. Finally, the safety and licensing issues that affect the use of Bacillus species for commercial development will be summarized, together with evidence showing the growing need to evaluate the safety of individual Bacillus strains as well as species on a case by case by basis.     

肠道菌群及内毒素在多器官功能不全综合征时的变化

目的　探讨肠道菌群及内毒素在多器官功能不全综合征( MODS)时的变化。方法　取SD大鼠,腹腔注射无菌酵母多糖A制备MODS模型,检测大鼠肠道菌群、外周血和门静脉血中的内毒素以及肠道游离内毒素含量,并进行定量分析。结果　模型组大鼠肠道专性厌氧菌的数量明显减少,革兰阴性杆菌和双歧杆菌的比例倒置,内毒素含量明显增加,与对照组比差异有显著性( P<0 .0 5 )。结论　MODS时肠道细菌微生态发生明显改变,肠道内毒素池与肠道革兰阴性杆菌的变化密切相关