Eggs of pelagic fish from the Sargasso Sea with special reference to Anguilliformes

Pelagic fish eggs from IKMT hauls, taken during the 1979 Sargasso Sea Expedition, were sorted and classified according to structural aspects. 13 types were distinguished, one of them classified as exocoetid, two of them as anguilliform. One of the anguilliform egg types could be determined as belonging toDerichthys serpentinus.     

Consumption of benthic fauna by carnivorous birds in the Wadden Sea

Consumption by carnivorous birds was estimated for the Sylt-Rømø tidal inlet in the northern part of the Wadden Sea, as well as the subarea Königshafen, a small, tidal bay. The bird community of the Sylt-Rømø Wadden Sea was dominated by Dunlin (35% of all birds counted), Eider (9%), Oystercatcher (8%), Knot (8%), and Shelduck (7%). The community in the Königshafen was dominated by Eider (20%), Knot (17%), Bar-tailed Godwit (17%), Dunlin (13%), and Oystercatcher (8%). Annual consumption was estimated at 3.4 g AFDW · m^?2 · year^?1 for the entire Sylt-Rømø Wadden Sea and 19.2 g AFDW · m^?2 · year^?1 for the Königshafen. Restricting the calculations to the intertidal area resulted in a consumption of 8.7 g AFDW · m^?2 · year^?1 for the Sylt-Rømø Wadden Sea and 17.6 g AFDW · m^?2 · year^?1 for the Königshafen. In the two areas, consumption was dominated by the Eider with 37% and 60% of the total consumption, respectively. In comparison to the western parts of the Wadden Sea the seasonal pattern of consumption as well as species composition differed, most probably as an effect of different climatic conditions, whereas annual consumption on intertidal flats seems to be in the same order of magnitude. On average, 15–25% of the mean annual macrozoobenthic biomass seems to be taken by carnivorous birds in the Wadden Sea, which is in the same order of magnitude as in other northern temperate estuarine areas.     

Keratinization of fish skin with special reference to the catfish Bagarius bagarius

Summary Histochemical reactions indicating keratinization have previously been demonstrated in parts of the epidermis of Bagarius bagarius. Fluorescence histochemistry and electron microscopy have now confirmed these results. Elevated areas of the epidermis are capped by a layer of dead cells with altered contents. On the outer aspect of these cells a dense layer, 18 nm thick, beneath the plasma membrane corresponds to the resistant envelope found in keratinized cells in tetrapod vertebrates. In Bagarius this layer does not extend to all faces of the keratinized cells, but a similar envelope has been detected in two other sites of piscine keratinized epidermis investigated, namely in the breeding tubercles of Phoxinus phoxinus and in the teeth of Lampetra fluviatilis. In the elevated areas of Bagarius-epidermis, the epithelial cells undergo progressive changes in cytoplasmic organization as they become more superficial. The second tier from the surface is sealed by tight junctions and is separated from the overlying keratinized cells by a sub-corneal space resembling that found in keratinized amphibian epidermis. Histochemical evidence of a high lipid content in the outer layers of the epidermis correlates with the presence of lipid inclusions and lamellated membranous profiles in the material studied by electron microscopy. Histochemical results show that the fin skin of Blennius pholis is not keratinized, but secretes a cuticle, histochemically reactive for both proteins and glycoproteins.     

The dominant fish fauna in the North Sea and its determination

The 224 species of fish reported from the North Sea are regarded as being composed of elements of three faunas—Boreal, Lusitanian and Atlantic, and they can be grouped accordingly on both a number of species and a biomass basis. The estimation of biomass of more than 10 important species is based on stock estimates obtained from population data. For 65 non-standard species the estimate is based on a comparison of catch rates for 'standard'and non-standard species from groundfish survey data. This involves assumption about the relative catchability of different sets of species. For the other species the biomass are computed in different ways. An analysis of the dominant fish fauna in the North Sea is attempted. The fish fauna in the area is analysed in two ways: by the conventional total number of species from each zoogeographic area, and by the biomass of the representatives of each fauna. It is concluded that for establishing the dominant faunal element of fish, biomass is a better index than is the number of species. The dominant faunal element of fish in the North Sea is Boreal.     

Application of different methods for nuclear shape analysis with special reference to the differentiation of brain tumors

OBJECTIVE: To study the discriminatory power of different methods designed for nuclear shape analysis with reference to the differentiation and grading of brain tumors and the differentiation between proliferating and nonproliferating nuclei. STUDY DESIGN: At least 300 tumor cell nuclei per case were measured by means of a digital image analysis system. Fourier amplitudes no. 1 to 15, moments no. 1 to 7 according to Hu, roundness factor, ellipse shape factor, concavity factor, Feret ratio, fractal dimension and bending energy were determined for each nucleus. The discriminatory power of these parameters was tested in three pairwise comparisons: (1) oligodendrogliomas WHO grade II (n = 13) vs. grade III (n = 11), (2) medulloblastomas WHO grade IV (n = 14) vs. anaplastic ependymomas WHO grade III (n = 12), (3) Ki-67-positive vs. Ki-67-negative tumor cell nuclei in the 14 medulloblastomas. RESULTS: When data from Fourier analysis were included in statistical analysis, cross-validated discriminant analysis led to a 100% correct reclassification for the first and for the second pairwise comparison and to a 75% correct reclassification when comparing Ki-67-positive and Ki-67-negative nucleifrom medulloblastomas. Different combinations of the other shape parameters led to a lower percentage of correctly reclassified cases for all three pairwise comparisons, especially when Fourier analysis was not included in the analysis. CONCLUSION: Fourier analysis provided an optimal statistical discrimination between different brain tumor entities and between data sets from proliferating and nonproliferating tumor cell nuclei. Since nuclear shape is an important criterion for the investigation of tumors, the application of Fourier analysis is therefore recommended for quantitative histologic investigations in neuro-oncology.     

Chemical monitoring in the Dutch Wadden Sea by means of benthic invertebrates and fish

In monitoring, it is of utmost importance to carefully define the purpose, the sampling strategy, as well as the analytical chemical and statistical requirements. Surveys are appropriate for describing the geographical variation in environmental contaminant levels. Repeated surveys and recurrentdata collection at permanent locations provide means of detecting temporal trends. Results are presented here of surveys on pollution by trace metals, polychlorinated biphenyls and organochlorine pesticides in the Ems Estuary and Dutch Wadden Sea usingMytilus edulis, Mya arenaria, Arenicoia marina, Nereis diversicolor andCrangon crangon as test organisms. Trends towards decreasing pollution by mercury are illustrated by monitoring data onMytilus edulis andZoarces viviparus. It is stressed that the results of chemical monitoring in organisms may be interpreted only in termser the biological effects on the basis of relevant toxicological knowledge and/or additional bio-assays. Presented at the VI International Wadden Sea Symposium (Biologische Anstalt Helgoland Wattenmeerstation Sylt, D-2282 List, FRG, 1–4 November 1988)     

Cell volume regulation in fish heart ventricles with special reference to taurine

A review of the cell volume regulation mechanism in heart ventricles of teleosts reveal that the mechanism is not only restricted to euryhaline species in a changing salinity regime but also is manifested in fresh-water fish. Taurine is the dominating amino acid and the main cellular osmo-effector in teleost hearts (accounting for 40-50% of the osmolality change). During hypo-osmotic regulation, cellular taurine is reduced by an efflux from the cells, whereas intracellular synthesis of taurine most probably accompanies hyper-osmotic regulation. Vertebrate hearts seem to have a high concentration of taurine and it may in general in vertebrate hearts also play a pivotal role in cellular osmoregulatory function.     

The control of drinking in elasmobranch fish with special reference to the renin-angiotensin system

In summary, it is evident that teleost and elasmobranch fish respond to extra-cellular dehydration by increasing drinking rate mediated by an increase in circulating levels of ANG II. However, although the primary stimulus for drinking may be the same, clearly the mechanisms involved in regulating ion and water balance are entirely different. In order to maintain ion and water balance in the face of cellular and extra-cellular dehydration, the integration and hormonal control of renal and extra-renal function in elasmobranchs has developed in a very different manner to that described for teleost fish.     

Monitoring arthropods in a tropical landscape: relative effects of sampling methods and habitat types on trap catches

To discuss the challenge of monitoring multi-species responses of tropical arthropods to disturbance, we considered a large dataset (4 × 10⁵ individuals; 1,682 morphospecies representing 22 focal taxa) based on the work of parataxonomists to examine the effects of anthropogenic disturbance on arthropods at Gamba, Gabon. Replication included three sites in each of four different stages of forest succession and land use after logging, surveyed during a whole year with four sampling methods: pitfall, Malaise, flight-interception and yellow pan traps. We compared the suitability of each sampling method for biological monitoring and evaluated statistically their reliability for 118 arthropod families. Our results suggest that a range of sampling methods yields more diverse material than any single method operated with high replication. Multivariate analyses indicated that morphospecies composition in trap catches was more strongly influenced by habitat type than by sampling methods. This implies that for multi-species monitoring, differences in trap efficiency between habitats may be neglected, as far as habitat types remain well contrasted. We conclude that for the purpose of monitoring large arthropod assemblages in the long-term, a protocol based on operating a set of different and non-disruptive traps appears superior in design than summing a series of taxa-specific protocols.     

Biological consequences of marine pollution with special reference to the North Sea fisheries

Summary 1. A case of copper pollution on the Dutch coast is used to demonstrate that waste disposal in inshore waters can easily lead to damage to the living resources of the sea, since dilution is under such conditions a much slower process than is usually assumed.2. Pollution by domestic sewage, its direct hazards for human health, and its contaminating effect on shellfish, is discussed. Next to these direct and indirect health hazards, discharge of domestic waste may lead to eutrophication, which in its turn can contribute to the development of plankton blooms producing truly poisonous metabolites. Some examples of eutrophication of the marine environment are given.3. Of the industrial waste, examples are given which clearly demonstrate how dangerous discharge of heavy metals and pesticides can be, also in the marine environment. Possible effects of petrochemical waste products and of synthetic detergents and soaps are discussed.4. Radio-active waste disposal in sea is more carefully studied and monitored than disposal of other types of waste.5. The increasing quantity and variety of industrial waste is a reason for serious concern. Careful discrimination between the many products is advocated and discharge far offshore is advised for certain categories of pollutants. Pesticides and other very dangerous products should never be admitted in the shallow sea areas, so important as nursery grounds for the fishery resources.

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Biologische Konsequenzen der Meerwasserverschmutzung unter besonderer Berücksichtigung der Nordseefischerei

Kurzfassung Obwohl das Gesamtvolumen der Ozeane erheblich größer ist als der Süßwasservorrat der Welt, so kann doch das hemmungslose Einbringen von Haus- und Industrieabwässern in das Meer zu schädlichen Folgen für den Bestand der Meeresflora und -fauna führen. Besonders anfällig sind die Küstengewässer, da diese oft die Ernährungsgrundlage für viele Nutzfische bieten und zudem dort Austern und Miesmuscheln gezüchtet werden. Aus wirtschaftlichen Gründen erfolgt andererseits das Verklappen von Abfall und Unrat vorzugsweise dicht unter der Küste. Schädliche Wirkungen können erfolgen durch Bakterien- und Virusinfektionen, durch biologische Akkumulation von Schwermetallen und von Schädlingsbekämpfungsmitteln (unter ihnen besonders chlorhaltige Kohlenwasserstoffe), durch petrochemische Produkte, wodurch übler Geschmack bei Fischen und Muscheln hervorgerufen wird, und durch eine allgemeine Eutrophierung, die zu einer Vermehrung von unerwünschtem Plankton führen kann.

     

Developmental plasticity in the cardiovascular system of fish,with special reference to the zebrafish

During development the circulatory system of vertebrates typically starts operating earlier than any other organ. In these early stages, however, blood flow is not yet linked to metabolic requirements of tissues, as is well established for adults. While the autonomic nervous system becomes functional only quite late during development, in the early stages control of blood flow appears to be possible by blood-borne and/or local hormones. This study presents methods based on video-imaging techniques and fluorescence microscopy to visualize cardiac activity, as well as the vascular bed of developing lower vertebrates, and tests the idea that environmental factors, such as hypoxia, may modify cardiac activity, or even the early formation of blood vessels in embryos and larvae. In zebrafish larvae, adaptations of cardiovascular activity to chronic hypoxia become visible shortly after hatching, and the formation of some blood vessels is enhanced under chronic hypoxia. Exposure of early larval stages of zebrafish to a constant water current induces physiological adaptations, resulting in enhanced swimming efficiency and increased tolerance towards hypoxia. Furthermore, application of hormones such as NO can modify cardiac activity as well as peripheral resistance, and they can stimulate blood vessel formation. In consequence, even during early development of fish or amphibian larvae, the performance of cardiac muscle and of skeletal muscle can be modified by environmental influences and peripheral resistance can be adjusted. Even blood vessel formation can be stimulated by hypoxia, for example, or by the presence of specific hormones. Thus, at approximately the time of hatching the physiological performance of vertebrate larvae is already determined by the combined action of environmental influences and of genetic information.     

Influence of light quality and gassing on the vertical migration of diatoms inhabiting the Wadden Sea

Diatoms inhabiting the Wadden Sea show a rhythmic migration pattern, which is superimposed by the tidal rhythm. In addition to light intensity, light quality has a pronounced influence on the upward-directed migration, thus giving some information on the nature of the relevant photoreceptors. Maximum diatom migration occurred when sediment surfaces were illuminated with blue light. The cell densities in blue light exceeded those of white light control experiments 1.8-fold. Furthermore, we registered a minor peak in the red light region, which reached approximately 60% of the white light controls. Cryptochrome and/or phototropin may thus be involved and act as photoreceptors for the vertical migration pattern. Flushing sediment surfaces of freshly mixed Wadden Sea sediments with air, O₂, CO₂ or N₂ did not show a significant influence of O₂ on the upward migration. The disappearance of diatoms from sediment surfaces which were flushed with CO₂ is most probably caused by the acidification of the sediment bed.Communicated by E. Rachor