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The low oxygen,oxidative and osmotic stress responses synergistically act through the ethylene response factor VII genes RAP2.12, RAP2.2 and RAP2.3 下载免费PDF全文
Csaba Papdi Imma Pérez‐Salamó Mary Prathiba Joseph Beatrice Giuntoli László Bögre Csaba Koncz László Szabados 《The Plant journal : for cell and molecular biology》2015,82(5):772-784
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Abiotic stresses have adverse effects on plant growth and productivity. The homologous RD29A and RD29B genes are exquisitely sensitive to various abiotic stressors. Therefore, RD29A and RD29B gene sequences have potential to confer abiotic stress resistance in crop species grown in arid and semi-arid regions. To
our knowledge, no information on the physiological roles of the proteins encoded by RD29A and RD29B are available in the literature. To understand how these proteins function, we used reverse genetic approaches, including
identifying rd29a and rd29b T-DNA knockout mutants, and examining the effects of complementing transgenes with the genes under control of their native
promoters and chimeric genes with the native promoters swapped. Four binary vectors with the RD29A and RD29B promoters upstream of the cognate RD29A and RD29B cDNAs and as chimeric genes with noncognate promoters were used to transform rd29a and rd29b plants. Cold, drought, and salt induced both genes; the promoter of RD29A was found to be more responsive to drought and cold stresses, whereas the promoter of RD29B was highly responsive to salt stress. Morphological and physiological responses of rd29a and rd29b plants to salt stress were further investigated. Root growth, and photosynthetic properties declined significantly, while
solute concentration (Ψπ), water use efficiency (WUE) and δ13C ratio increased under salt stress. Unexpectedly, the rd29a and rd29b knockout mutant lines maintained greater root growth, photosynthesis, and WUE under salt stress relative to control. We conclude
that the RD29A and RD29B proteins are unlikely to serve directly as protective molecules. 相似文献
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Huaying Liu Wenlong Yang Dongcheng Liu Yuepeng Han Aimin Zhang Shaohua Li 《Molecular biology reports》2011,38(1):417-427
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Caiqiu Gao Yucheng Wang Guifeng Liu Chao Wang Jing Jiang Chuanping Yang 《Plant Molecular Biology Reporter》2010,28(1):77-89
Plant peroxidases (PODs) have been ascribed a variety of biological functions, including hydrogen peroxide detoxification,
lignin biosynthesis, hormonal signaling, and stress response. In the present study, ten POD genes, including three ascorbate peroxidases (class I PODs) and seven secretory peroxidases (class III PODs), were cloned from Tamarix hispida. The roles of the ten POD genes were addressed under different abiotic stress conditions, and gene expression profiles in roots, stems, and leaves
were evaluated using real-time quantitative reverse-transcribed polymerase chain reaction. Our results showed that the relative
abundance of the PODs was markedly different in roots, stems, and leaves, indicating that POD activity differs in these three organs. ThPOD1 and ThPOD8 were the most and least abundant, respectively, in all organs. The expression profiles in response to abiotic stresses were
organ specific. All of the genes were highly induced by drought, salt, salt–alkaline, CdCl2, and abscisic acid (ABA) treatments in at least one organ. Five ThPOD genes were induced in roots, stems, and leaves under all of the studied stress conditions, indicating that they are closely
associated with abiotic stress. Our results demonstrate that the ten plant peroxidases are all expressed in leaves, stems,
and roots, that they are involved in different abiotic stress responses, and that they are controlled by an ABA-dependent
stress signaling pathway. 相似文献
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Overexpression of the trehalose-6-phosphate synthase gene <Emphasis Type="Italic">OsTPS1</Emphasis> enhances abiotic stress tolerance in rice 总被引:1,自引:0,他引:1
Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. In plants,
its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase
(TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity. To demonstrate the physiological function of OsTPS1, we introduced it into rice
and found that OsTPS1 overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant
phenotypic changes. In transgenic lines overexpressing OsTPS1, trehalose and proline concentrations were higher than in the wild type and some stress-related genes were up-regulated,
including WSI18, RAB16C, HSP70, and ELIP. These results demonstrate that OsTPS1 may enhance the abiotic stress tolerance of plants by increasing the amount of trehalose
and proline, and regulating the expression of stress-related genes. Furthermore, we found that overexpression of some Class
II TPSs also enhanced plant tolerance of abiotic stress. This work will help to clarify the role of trehalose metabolism in abiotic
stress response in higher plants. 相似文献
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Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes 总被引:4,自引:0,他引:4
Satoko Matsukura Junya Mizoi Takumi Yoshida Daisuke Todaka Yusuke Ito Kyonoshin Maruyama Kazuo Shinozaki Kazuko Yamaguchi-Shinozaki 《Molecular genetics and genomics : MGG》2010,283(2):185-196
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