共查询到18条相似文献，搜索用时 57 毫秒
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刘智任陈冬磊 《现代生物医学进展》2012,12(16):3182-3184
聚己内酯(PCL)以其具有的良好生物相容性及其力学特点,在组织工程领域已经成为主要的生物支架材料之一。利用生物支架材料,组织工程的目的是对组织、器官的丧失或功能障碍进行修复与重建。本文综述了对生物支架材料聚己内酯(PCL)的研究进展以及其在组织工程中的应用。 相似文献
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干细胞联合生物支架材料体外构建功能性组织与器官,成为当前组织再生研究的重要策略,而探求具有良好生物相容性的支架材料是其关键.本研究采用扫描电镜、噻唑蓝（MTT）法、荧光显微染色等方法检测小鼠诱导多能干细胞（murine induced pluripotent stem cells, miPSCs）在聚己内酯（poly ε-caprolactone, PCL）静电纺丝纳米纤维支架上的粘附、增殖等生物学特性,探究聚己内酯纳米纤维支架与miPSCs的生物相容性. 结果显示,miPSC在PCL纳米纤维支架上具有良好粘附性并呈集落样生长,其增殖能力及干性标记物（Oct4-GFP+）的表达均不亚于标准对照组;扫描电镜显示,miPSC在PCL纳米纤维支架材料上呈现出绒毛状突起的表面结构.上述结果表明,PCL纳米纤维支架可促进miPSCs的粘附、自我增殖以及干性维持,两者具有良好的生物相容性,为下一步联合生物支架材料与干细胞构建功能性组织奠定了基础. 相似文献
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目的：探讨利用生物可降解支架修复动物胸骨缺损,为临床手术治疗提供新的可行性方法。方法：对于12只比格犬进行手术切除部分胸骨,并利用聚己内酯/羟基磷灰石（PCL/HA）复合支架,并制备出与临床相似的胸骨缺损模型。实验动物分成2组,分别是：空白对照组和PCL/HA支架组。分别于术后第4、12周进行胸部CT扫描,并对胸廓进行三维重建,观察胸骨缺损部位的修复情况,并在第12周取胸骨缺损部位组织进行硬组织切片,苦味酸-品红染色,观察缺损部位的骨组织修复情况,并利用软件进行骨组织比率分析,评估修复情况。结果：通过检查发现空白对照组的胸骨缺损部位未见明显骨连接,胸廓的骨性结构有明显畸形,PCL/HA支架组能很好地维持胸廓的完整性,组织学检查发现PCL/HA支架组的缺损部位有明显新生骨形成,通过软件分析可发现支架组的骨组织比率较空白组的高（P〈0.05）。结论：这些结果表明采用PCL/HA复合材料支架能很好地修复胸骨缺损。 相似文献
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目的采用可降解的聚己内酯接枝肝素材料,负荷b-FGF(碱性成纤维细胞生长因子),体外构建的小口径组织工程血管,完成犬的股动脉移植动物实验。方法利用可降解的聚己内酯接枝肝素材料,电纺丝技术制备组织工程血管支架,并对支架负荷b-FGF生长因子,并进行材料的内皮细胞粘附实验。将体外构建的小口径组织工程血管,完成犬的股动脉移植动物实验,观察通畅率和移植术后组织工程血管的改变。结果可降解聚己内酯接枝肝素材料支架,负荷细胞生长因子(b-FGF),利于内皮细胞粘附。构建的组织工程血管进行体外动物实验构建,3个月移植物通畅率好,移植后取材,有新生内膜迁移和胶原纤维浸入。结论利用可降解聚己内酯接枝肝素材料构建小口径支架,初步符合构建组织工程血管支架的要求。 相似文献
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目的探讨聚己内酯(PCL)/柚皮素纳米纤维膜对白介素1β(IL-1β)诱导SD大鼠软骨细胞退行性变修复作用。方法使用静电纺丝技术分别制作PCL纳米纤维膜,PCL/柚皮素纳米纤维膜,使用扫描电子显微镜(SEM)观察纳米纤维膜的表征。提取3～5 d的SD大鼠软骨细胞,分为空白对照组、骨关节炎(OA)组、骨关节炎加PCL(OA+PCL)组、骨关节炎加PCL/柚皮素(OA+PCL/柚皮素)组,分别处理24 h后进行CCK-8实验检测各组SD大鼠软骨细胞的增殖情况,并进行活/死细胞染色,观察各组SD大鼠软骨细胞的毒性。提取各组SD大鼠软骨细胞的总RNA,使用qRT-PCR检测炎症及软骨标志基因的表达。结果与OA组相比,PCL/柚皮素纳米纤维膜组大鼠的细胞活性较高,细胞数量明显增多,炎症标志基因表达明显降低,软骨标志基因表达明显增高(均P<0.05)。结论 PCL/柚皮素能降低IL-1β诱导的SD大鼠体外骨关节炎症标志基因的表达,对SD大鼠关节炎有一定治疗作用,且能上调关节炎细胞中软骨标志基因COL2a1的表达。 相似文献
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目的:观察聚己内酯/壳聚糖神经导管复合骨髓间充质干细胞修复大鼠坐骨神经缺损的效果。方法:将24只SD大鼠随机分为4组,制备右侧坐骨神经5mm缺损模型,A组聚己内酯/壳聚糖神经导管复合骨髓间充质干细胞移植组;B组聚己内酯神经导管复合骨髓间充质干细胞移植组;C组壳聚糖神经导管复合骨髓间充质干细胞移植组;D组自体神经移植组。术后每2周进行坐骨神经功能指数检测,12周时行电生理、腓肠肌湿重恢复率、组织学观察和免疫组织化学检测。结果:坐骨神经功能指数显示,A组运动功能恢复速度较B、C组快,但比D组慢。A组电生理和腓肠肌湿重恢复率的检测结果与C、D组相比无统计学意义(P0.05),但优于B组(P0.05)。组织学观察,A组再生神经纤维排列密集。S-100免疫组织化学结果表明A组有大量雪旺细胞增生。结论:聚己内酯/壳聚糖神经导管复合骨髓间充质干细胞能够促进周围神经损伤修复,效果与壳聚糖神经导管、自体神经相同,优于聚己内酯神经导管。 相似文献
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由环己酮生物合成己内酯的研究 总被引：5，自引：0，他引：5
从本实验室保藏菌种中分离筛选出一株能够以环己酮作为唯一碳源的菌株,初步鉴定为邻单胞菌属,并对其产物进行CC/MS定性,发现有己内酯生成,存在Baeyer-villiger反应.文章还探讨了pH,装液量,底物浓度,培养时间,温度以及转速等条件对细菌生长的影响,并进一步研究了细菌的底物广谱性,发现此菌能够利用与环己酮具有相似结构的环戊酮等有机物. 相似文献
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心血管疾病是一种常见的疾病,目前尚缺乏能够应用于冠脉搭桥手术的小口径人工血管。传统的组织工程血管支架制备技术在调节支架的孔径、几何形态和互连性方面上存在不足。3D生物打印技术能够模拟血管组织的天然结构,精确打印活细胞和生物材料,在纳米尺度上对支架的微观结构和孔隙率进行调控,为研发新型的组织工程血管提供了新思路。本文系统评价了3D生物打印技术的分类特点,深入探讨了3D生物打印技术在组织工程血管领域的最新研究进展,分析总结了其优点,同时指出此技术还存在较多问题需要解决,如血管材料的免疫排斥等,为其进一步的研究提供参考和借鉴。 相似文献
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形状记忆聚合物是由固定相和可逆相构成的具有在外界刺激条件下诱导形状改变特性的一类高分子智能材料。相较于传统的形状记忆合金与陶瓷,其具有特定的生物可降解性、更高的机械性能调控空间、更强的形变恢复能力及更优良的生物相容性。凭借材料特性,近阶段针对形状记忆聚合物在组织工程领域的应用研究愈发广泛,包括血管组织、骨骼肌组织、神经组织与骨组织等方面。综述近年来形状记忆聚合物在多种组织工程领域研究中的实验创新、技术突破与应用拓展,例如将其作为新型多孔血管支架、骨骼肌修复支架、神经修复导管与骨缺损填充物等。可预见随着技术和材料的不断发展,形状记忆聚合物在组织工程领域的应用将更加成熟。 相似文献
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关节软骨损伤后的自我修复是医学界一直在研究和探讨的难题。3D生物打印技术可以精准的分配载细胞生物材料,构建复杂的三维活体组织,在优化软骨缺损修复组织的内部结构、机械性能以及生物相容性上有很大优势,因此近年来成为软骨修复组织工程领域的研究热点。重点介绍了软骨生物3D生物打印的最新进展,包括软骨生物打印“墨水”材料的选择、种子细胞的来源以及3D生物打印技术的发展。此外,还阐述了3D生物打印技术在组织工程学应用上的部分局限性,并对其在软骨修复领域的发展与应用进行了预测。 相似文献
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化石的三维复原与实物打印技术是建立古生物学研究与科普之间的重要桥梁。然而, 与常为三维立体保存的脊椎动物化石相比, 植物化石受自身生物结构的影响, 多以二维状态保存, 三维复原技术难度大。为解决此问题, 本文以柴达木盆地中新世德令哈莲Nelumbo delinghaensis Luo et Jia, 2022为材料, 探索并优化基于3D打印技术的古植物复原方法, 以期为古植物学研究与科普提供新的三维复原方案和展示途径。本文确立了针对古植物压型或印痕化石的普适性3D打印技术, 包含提取化石性状信息、图像处理、数字建模、3D打印、打印后处理等。此外, 还针对植物化石的特性, 提出了打印及后处理过程的优化方案, 如抽壳处理、添加晶格结构、模型拆分、化石性状细节复原优化等, 从而提升三维复原品的真实感与科学性。 相似文献
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Biodegradable polycaprolactone and collagen nanofibers were produced by electrospinning, with fiber diameters of around 300-700nm and features similar to the extracellular matrix of natural tissue. Human coronary artery smooth muscle cells (SMCs) seeded on nanofibrous matrices tend to maintain normal phenotypic shape and growth tends to be guided by the nanofiber orientation. The SMC and nanofibrous matrix interaction was observed by SEM, MTS assay, trypan blue exclusion method and laser scanning confocal microscopy. The results showed that the proliferation and growth rate of SMCs were not different on polycaprolactone (PCL) nanofibrous matrices coated with collagen or tissue culture plates. PCL nanofibrous matrices coated with collagen showed that the SMCs migrated towards inside the nanofibrous matrices and formed smooth muscle tissue. This approach may be useful for engineering a variety of tissues in various structures and shapes, and also to demonstrate the importance of matching both the initial mechanical properties and degradation rate of nanofibrous matrices to the specific tissue engineering. 相似文献
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Extrusion‐based bio‐printing has great potential as a technique for manipulating biomaterials and living cells to create three‐dimensional (3D) scaffolds for damaged tissue repair and function restoration. Over the last two decades, advances in both engineering techniques and life sciences have evolved extrusion‐based bio‐printing from a simple technique to one able to create diverse tissue scaffolds from a wide range of biomaterials and cell types. However, the complexities associated with synthesis of materials for bio‐printing and manipulation of multiple materials and cells in bio‐printing pose many challenges for scaffold fabrication. This paper presents an overview of extrusion‐based bio‐printing for scaffold fabrication, focusing on the prior‐printing considerations (such as scaffold design and materials/cell synthesis), working principles, comparison to other techniques, and to‐date achievements. This paper also briefly reviews the recent development of strategies with regard to hydrogel synthesis, multi‐materials/cells manipulation, and process‐induced cell damage in extrusion‐based bio‐printing. The key issue and challenges for extrusion‐based bio‐printing are also identified and discussed along with recommendations for future, aimed at developing novel biomaterials and bio‐printing systems, creating patterned vascular networks within scaffolds, and preserving the cell viability and functions in scaffold bio‐printing. The address of these challenges will significantly enhance the capability of extrusion‐based bio‐printing. 相似文献
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The tooth and its supporting tissues are organized with complex three-dimensional (3D) architecture, including the dental pulp with a blood supply and nerve tissues, complex multilayer periodontium, and highly aligned periodontal ligament (PDL). Mimicking such 3D complexity and the multicellular interactions naturally existing in dental structures represents great challenges in dental regeneration. Attempts to construct the complex system of the tooth and tooth-supporting apparatus (i.e., the PDL, alveolar bone, and cementum) have made certain progress owing to 3D printing biotechnology. Recent advances have enabled the 3D printing of biocompatible materials, seed cells, and supporting components into complex 3D functional living tissue. Furthermore, 3D bioprinting is driving major innovations in regenerative medicine, giving the field of regenerative dentistry a boost. The fabrication of scaffolds via 3D printing is already being performed extensively at the laboratory bench and in clinical trials; however, printing living cells and matrix materials together to produce tissue constructs by 3D bioprinting remains limited to the regeneration of dental pulp and the tooth germ. This review summarizes the application of scaffolds for cell seeding and biofabricated tissues via 3D printing and bioprinting, respectively, in the tooth and its supporting tissues. Additionally, the key advantages and prospects of 3D bioprinting in regenerative dentistry are highlighted, providing new ideas for dental regeneration. 相似文献
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The last few decades have witnessed diversified in vitro models to recapitulate the architecture and function of living organs or tissues and contribute immensely to advances in life science. Two novel 3D cell culture models: 1) Organoid, promoted mainly by the developments of stem cell biology and 2) Organ-on-a-chip, enhanced primarily due to microfluidic technology, have emerged as two promising approaches to advance the understanding of basic biological principles and clinical treatments. This review describes the comparable distinct differences between these two models and provides more insights into their complementarity and integration to recognize their merits and limitations for applicable fields. The convergence of the two approaches to produce multi-organoid-on-a-chip or human organoid-on-a-chip is emerging as a new approach for building 3D models with higher physiological relevance. Furthermore, rapid advancements in 3D printing and numerical simulations, which facilitate the design, manufacture, and results-translation of 3D cell culture models, can also serve as novel tools to promote the development and propagation of organoid and organ-on-a-chip systems. Current technological challenges and limitations, as well as expert recommendations and future solutions to address the promising combinations by incorporating organoids, organ-on-a-chip, 3D printing, and numerical simulation, are also summarized. 相似文献
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