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Wen Zhao PhD Yijuan Han PhD Dongyan Shao PhD Cuicui Han PhD Yixiao Tian PhD Qingsheng Huang PhD 《Bioelectromagnetics》2023,44(7-8):211-220
To explore the effect of ultra-strong static magnetic field on gut microbiota, 16 T static magnetic field was used to study the changes in the structure and composition of human and mouse gut microbiota in this environment. In the mouse gut microbiota, at the genus level, the magnetic field significantly decreased the relative abundances of Escherichia-Shigella, Lactobacillus, Enterococcus, Burkholderia-Caballeronia-Paraburkholderia, Parasutterella, and Ralstonia and significantly increased those of Parabacteroides, Alloprevotella, Alistipes, Odoribacter, Bacteroides, Mucispirillum, Sutterella, and Prevotellaceae_UCG-001. Similarly, at the genus level, the relative abundances of Bacteroides, Parabacteroides, Romboutsia, and Streptococcus significantly decreased in the human gut microbiota. Contrary to the changing trend of the abundance in the mouse gut, the abundances of Bacteroides and Parabacteroides in the human gut were significantly reduced under magnetic field. The BugBase phenotypic prediction analysis showed that the relative abundances of five phenotypes, including anaerobism, mobile elements, potential pathogenicity, stress-tolerant, and biofilm formation, changed significantly in the mouse gut microbiota, while the relative abundances of two phenotypes, including Gram-positive and Gram-negative phenotypes, changed significantly in the human gut microbiota. The 16 T magnetic field could differently affect the composition, structure, and phenotypes of gut microbiota in human and mice, suggesting the importance of model selection in studying the biological effects of magnetic field. 相似文献
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为了调查抑郁症患者的肠道微生物特征并验证肠道微生物是否可以成为抑郁症干预的新靶点,本研究共收集19例抑郁症患者和20例健康人的粪便样本进行16S rRNA高通量测序,并采用抑郁症自我评估量表(PHQ-9)、广泛性焦虑障碍量表(GAD-7)对参与者进行评估。基线微生物群分析显示,与健康人相比,抑郁症患者肠道菌群的Alpha多样性无群落级差异。差异物种分析表明,在抑郁症组中存在大芬戈尔德菌(Finegoldia)、干酪乳杆菌(Lactiaseibacillus)、肠杆菌目(Enterobacterales)、肠杆菌科(Enterobacteriaceae)、纺锤链杆菌属(Fusicatenilbacter)、梭状芽孢杆菌(Clostridia) 6种标志性微生物。相关性分析表明,健康组中显著富集的粪球菌属(Coprococcus)与抑郁焦虑水平呈明显负相关。本研究基于肠道微生物16SrRNA的高通量测序来解析抑郁症患者的肠道菌群结构和组成,并为缓解抑郁症的临床症状提供了潜在的微生物干预靶点。 相似文献
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昆虫肠道微生物多样性对宿主的生长发育以及营养物质的消化和吸收等起着非常重要的作用。本研究利用16S rRNA基因序列构建神农洁蜣螂Catharsius molossus雌性成虫和雄性成虫肠道微生物的克隆文库,旨在探究其菌群的结构并加以对比和分析,采用Illumina NovaSeq高通量测序的方法揭示两种菌群组成方面的相似性和差异性。结果表明：神农洁蜣螂雌性成虫和雄性成虫肠道微生物在结构和组成方面存在着一定的差异。雌性成虫肠道菌群的物种丰富度和物种多样性都高于雄性成虫。雌性成虫、雄性成虫体内肠道菌群共有1 253个ASVs,归属于20个门、35个纲、54个目、134个科、253个属,其中雌性成虫肠道菌群的ASVs有1 024个,而雄性成虫肠道菌群的ASVs有410个,二者优势菌群虽然都为阴道球菌属Vagococcus,但二者所占的比例不同。本研究分析了神农洁蜣螂雌雄成虫肠道微生物多样性及群落在结构和组成方面的差异,为探究神农洁蜣螂雌雄成虫的肠道微生物的潜在功能,以及其肠道菌群的开发应用提供了理论支持。 相似文献
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【背景】小气道免疫球蛋白A (immunoglobulin A,IgA)在慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)的病理生理学中发挥着重要作用。然而肠黏膜来源的IgA在COPD病程中的作用及包被微生物群尚不明确。【目的】探讨COPD小鼠肠来源IgA包被肠道微生物组成、丰度及菌群基因功能变化。【方法】采用鼻腔滴入脂多糖和熏香烟法相结合建立COPD小鼠模型。收集COPD小鼠和野生型小鼠粪便样品各12份,IgA磁珠分选IgA包被的肠道微生物菌群,16S rRNA基因高通量测序分析其组成及功能。【结果】通过比较两组肺组织切片染色、平均内衬间隔(mean linear intercept,mLI)和肺泡灌洗液炎症因子浓度证实COPD模型鼠建模成功。OTU和主成分分析(principal component analysis,PCA)均发现两组间粪便样品中肠来源IgA包被微生物群落差异大,具有可比性。α多样性分析显示两组间物种多样性无显著统计学差异(P>0.05)。物种组成分析显示：两组肠来源IgA包被的菌群结构和菌群差异具有统计学意义(P<0.05)。COPD组的菌群结构中显著富集的菌目是：蛭弧菌目(Bdellovibrionales)、梭菌目(Clostridiales)和双歧杆菌目(Bifidobacteriales);科层面分类中富集的主要是：普雷沃氏菌科(Prevotellaceae)、梭菌科(Clostridiaceae)、类芽孢杆菌科(Paenibacillaceae)、蛭弧菌科(Bdellovibrionaceae)及双歧杆菌科(Bifidobacteriaceae);菌属层面分类上主要富集拟普雷沃氏菌属(Alloprevotella)、短芽孢杆菌属(Brevibacillus)、狭义梭菌属(Clostridium-sensu-stricto)、苏黎世杆菌属(Turicibacter)、粪杆菌属(Faecalibacterium)、吸血弧菌属(Vampirovibrio)和双歧杆菌属(Bifidobacterium)。菌群差异基因通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)数据库通路富集分析结果显示COPD组细胞生长与死亡、核苷酸代谢以及消化系统相关通路明显上调,而膜运输相关通路显著下调。【结论】COPD小鼠肠来源IgA包被肠道微生物存在紊乱及基因功能失调。 相似文献
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肠道菌群与动物营养代谢、机体健康、免疫等方面紧密相关,同时也可以间接反映物种的环境适应能力。2017年獐(Hydropotes inermis)的足迹在东北地区重新出现,初步探究其种群扩散、恢复的原因,并进一步为有效扩大獐在我国的分布,于2021年冬季通过无损伤取样法在辽宁省东南部四个地区采集獐新鲜粪便样本,采用高通量测序技术对粪便DNA中细菌16Sr RNA的V3-V4高变区进行扩增,对獐肠道菌群的组成及多样性进行分析。结果显示:厚壁菌门(Firmicute)和拟杆菌门(Bacteroidete)是獐肠道中的优势菌门,变形菌门(Proteobacteria,30.40%)和大肠杆菌志贺菌属(Escherichia_Shigella,28.48%)仅在沙尖子(SJZ)地区的相对丰度较大,而在其它三个地区中丰度分别不到2%和0.1%。四个地区獐的肠道微生物在丰富度上没有差异(ACE和Chao1,P ＞ 0.05),而獐肠道菌群多样性比较中,沙尖子地区与其它三个地区存在显著差异(Shannon和Simpson,P ＜ 0.05)。NMDS分析和ANOSIM分析结果显示,沙尖子地区獐肠道菌群与其它三地区在组内结构相似,但组间存在着一定差异(R ＞ 0,P ＜ 0)。LDA直方图表明四个地区的獐肠道中存在显著差异的细菌菌属有18种,Anaerobutyricum菌属在下露河地区獐的肠道中被显著富集,沙尖子地区中对组间差异影响最大的是近芽孢杆菌属(Peribacillus)。通过对东北地区分布的獐肠道菌群的分析,初步了解獐在寒冷地区生存的肠道菌群的适应机制,揭示獐体内的潜在致病菌和通过消化粗纤维来获取能量的方式,为进一步探究东北地区獐肠道微生物生理生态适应提供基础资料。建议通过一定措施保护或恢复辽宁省东南部地区獐的食物来源,提升獐适宜生境内的生物多样性水平,从而在一定程度上有效保护该种群。 相似文献
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XueFei Wang HuaLing Wang XiaoYu Su Jie Zhang JiaWei Bai JianYong Zeng HuiPing Li 《Archives of insect biochemistry and physiology》2023,112(1):e21978
The Asian long-horned beetle, Anoplophora glabripennies (Motschulsky), is a destructive wood-boring pest that is capable of killing healthy trees. Gut bacteria in the larvae of the wood-boring pest is essential for the fitness of hosts. However, little is known about the structure of the intestinal microbiome of A. glabripennies during larval development. Here, we used Illumina MiSeq high-throughput sequencing technology to analyze the larval intestinal bacterial communities of A. glabripennies at the stages of newly hatched larvae, 1st instar larvae and 4th instar larvae. Significant differences were found in larval gut microbial community structure at different larvae developmental stages. Different dominant genus was detected during larval development. Acinetobacter were dominant in the newly hatched larvae, Enterobacter and Raoultella in the 1st instar larvae, and Enterococcus and Gibbsiella in the 4th instar larvae. The microbial richness in the newly hatched larvae was higher than those in the 1st and 4th instar larvae. Many important functions of the intestinal microbiome were predicted, for example, fermentation and chemoheterotrophy functions that may play an important role in insect growth and development was detected in the bacteria at all tested stages. However, some specific functions are found to be associated with different development stages. Our study provides a theoretical basis for investigating the function of the intestinal symbiosis bacteria of A. glabripennies. 相似文献
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Yongzhen Wu;Taoxiu Zhou;Chen Gu;Baofa Yin;Shengmei Yang;Yunzeng Zhang;Ruiyong Wu;Wanhong Wei; 《Ecology and evolution》2024,14(3):e11084
The gut microbiota of rodents is essential for survival and adaptation and is susceptible to various factors, ranging from environmental conditions to genetic predispositions. Nevertheless, few comparative studies have considered the contribution of species identity and geographic spatial distance to variations in the gut microbiota. In this study, a random sampling survey encompassing four rodent species (Apodemus agrarius, Cricetulus barabensis, Tscherskia triton and Rattus norvegicus) was conducted at five sites in northern China's farming–pastoral ecotone. Through a cross-factorial comparison, we aimed to discern whether belonging to the same species or sharing the same capture site predominantly influences the composition of gut microbiota. Notably, the observed variations in microbiome composition among these four rodent species match the host phylogeny at the family level but not at the species level. The gut microbiota of these four rodent species exhibited typical mammalian characteristics, predominantly characterized by the Firmicutes and Bacteroidetes phyla. As the geographic distance between populations increased, the number of shared microbial taxa among conspecific populations decreased. We observed that within a relatively small geographical range, even different species exhibited convergent α-diversity due to their inhabitation within the same environmental microbial pool. In contrast, the composition and structure of the intestinal microbiota in the allopatric populations of A. agrarius demonstrated marked differences, similar to those of C. barabensis. Additionally, geographical environmental elements exhibited significant correlations with diversity indices. Conversely, host-related factors had minimal influence on microbial abundance. Our findings indicated that the similarity of the microbial compositions was not determined primarily by the host species, and the location of the sampling explained a greater amount of variation in the microbial composition, indicating that the local environment played a crucial role in shaping the microbial composition. 相似文献
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Sara Santarossa Alexandra R. Sitarik Christine Cole Johnson Jia Li Susan V. Lynch Dennis R. Ownby Alex Ramirez Germaine LM. Yong Andrea E. Cassidy-Bushrow 《Journal of Exercise Nutrition & Biochemistry》2021,25(4):24
[Purpose] To determine whether physical activity (PA), primarily the recommended 60 minutes of moderate-to-vigorous PA, is associated with gut bacterial microbiota in 10-year-old children.[Methods] The Block Physical Activity Screener, which provides minutes/day PA variables, was used to determine whether the child met the PA recommendations. 16S rRNA sequencing was performed on stool samples from the children to profile the composition of their gut bacterial microbiota. Differences in alpha diversity metrics (richness, Pielou’s evenness, and Faith’s phylogenetic diversity) by PA were determined using linear regression, whereas beta diversity (unweighted and weighted UniFrac) relationships were assessed using PERMANOVA. Taxon relative abundance differentials were determined using DESeq2.[Results] The analytic sample included 321 children with both PA and 16S rRNA sequencing data (mean age [SD] =10.2 [0.8] years; 54.2% male; 62.9% African American), where 189 (58.9%) met the PA recommendations. After adjusting for covariates, meeting the PA recommendations as well as minutes/day PA variables were not significantly associated with gut richness, evenness, or diversity (p ≥ 0.19). However, meeting the PA recommendations (weighted UniFrac R2 = 0.014, p = 0.001) was significantly associated with distinct gut bacterial composition. These compositional differences were partly characterized by increased abundance of Megamonas and Anaerovorax as well as specific Christensenellaceae_R-7_group taxa in children with higher PA.[Conclusion] Children who met the recommendations of PA had altered gut microbiota compositions. Whether this translates to a reduced risk of obesity or associated metabolic diseases is still unclear. 相似文献
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[背景] 人体能量稳态失衡表现为体重过轻、超重和肥胖,肠道菌群与人体能量稳态的维持有关,但不同身体质量指数（Body Mass Index,BMI）人群的肠道菌群特征仍需进一步探究。[目的] 基于美国肠道计划公开数据库,解析4类BMI人群肠道菌群的特征,并探究4类BMI人群肠道菌群共存网络特征及差异,为基于肠道菌群来干预肥胖及体重过轻等不健康状态提供新的理论依据。[方法] 从美国肠道计划数据集中筛选具有BMI信息的肠道菌群样本,并根据世界卫生组织规定的BMI划分标准将筛选后的样本分为4类：体重过轻（BMI<18.5 kg/m2）,正常体重（18.5 kg/m22）,超重（25 kg/m22）,肥胖（BMI>30 kg/m2）;通过计算和比较肠道菌群的α多样性和β多样性探究4类BMI人群肠道菌群的整体结构特征及差异;通过多元线性回归模型对不同BMI分类与肠道菌群进行相关性分析,并且将地域、年龄、性别因素作为混杂因素加入到模型中进行校正;采用SparCC分别计算4类BMI人群肠道菌群中菌属相关性,并分别构建肠道菌群共存网络。[结果] 经过Wilcoxon秩和检验,发现体重过轻、超重、肥胖人群的肠道菌群α多样性都显著低于正常体重人群;β多样性分析结果表明4类BMI人群肠道菌群的整体结构存在显著差异;4类BMI人群肠道中厚壁菌门（Firmicutes）和拟杆菌门（Bacteroidetes）的相对含量无显著差异;通过MaAsLin分析,并且将地域、年龄、性别因素作为混杂因素加入到模型中进行校正,共得到49个与BMI类型显著相关的物种;4类BMI人群肠道菌群共存网络的拓扑结构具有一定差异,体重过轻和正常体重人群肠道菌群共存网络的复杂度较高,超重和肥胖人群肠道菌群共存网络的复杂度较低。[结论] 4类BMI人群肠道菌群的多样性、整体结构和共存网络间均存在差异。 相似文献
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分子生物学技术在胃肠道微生态中应用研究进展 总被引：9，自引：0，他引：9
哺乳动物胃肠道中栖息着大量的微生物(主要为细菌),它们在营养、生理、免疫等方面对宿主起着有益作用。传统上,胃肠道菌群研究主要依靠培养技术。近来,一些基于16S rRNA(DNA)的分子生物学技术已被广泛应用于胃肠道菌群的研究,这些技术主要有16S rDNA克隆基因文库、16S rDNA指纹技术、定量PCR技术、荧光原位杂交技术及基因芯片技术等。对这些用于胃肠道微生态研究的分子生物学技术作一综述。 相似文献
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Breast cancer (BC) and benign breast lesions (BBLs) are common diseases in women worldwide. The gut microbiota plays a vital role in regulating breast diseases’ formation, progression, and therapy response. Hence, we explored the structure and function of gut microflora in patients with BC and BBLs. A cohort of 66 subjects was enrolled in the study. Twenty-six subjects had BC, 20 subjects had BBLs, and 20 matched healthy controls. High throughput 16S ribosomal RNA (16S rRNA) gene sequencing technology was used to determine the microbial community structure. Compared with healthy individuals, BC patients had significantly lower alpha diversity indices (Sobs index, p = 0.019; Chao1 index, p = 0.033). Sobs and Chao1 indices were also lower in patients with BBLs than healthy individuals, without statistical significance (p = 0.279, p = 0.314, respectively). Both unweighted and weighted UniFrac analysis showed that beta diversity differed significantly among the three groups (p = 3.376e–14, p < 0.001, respectively). Compared with healthy individuals, the levels of Porphyromonas and Peptoniphilus were higher in BC patients (p = 0.004, p = 0.007, respectively), whereas Escherichia and Lactobacillus were more enriched in the benign breast lesion group (p < 0.001, p = 0.011, respectively). Our study indicates that patients with BC and BBLs may undergo significant changes in intestinal microbiota. These findings can help elucidate the role of intestinal flora in BC and BBLs patients. Open in a separate window 相似文献
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蜜蜂和熊蜂是重要的传粉昆虫, 对农业生产及生态平衡的维持具有重要作用。近年来, 研究发现蜜蜂及熊蜂肠道内含有大量微生物, 其组成简单、特异。正常的肠道微生物群落对蜜蜂的生长、激素调节、致病菌抵抗等具有重要作用。随着高通量测序的发展, 研究者们也可快速获得传粉蜂肠道微生物组成, 这给生物多样性和物种保护及蜂类健康等的研究带来了便捷。但是由于蜜蜂和熊蜂肠道微生物群落均由特殊菌种组成, 目前的细菌16S rRNA数据库无法对其进行准确的分类, 并且部分东方蜜蜂(Apis cerana)特有的肠道微生物菌种缺乏16S rRNA序列信息。本文从来源于5个不同省份的东方蜜蜂肠道中分离得到在东方蜜蜂中普遍含有的Apibacter菌属纯菌, 获取其全长16S rRNA序列, 并对目前蜜蜂和熊蜂肠道的5个核心菌种的分类进行了综述, 对其分类和命名进行了修正。根据蜜蜂肠道微生物的明确分类, 在目前常用的SILVA细菌分类数据库基础之上对其进行了命名及分类优化, 并加入东方蜜蜂中普遍含有的Apibacter序列, 从而获得了优化数据库Bee Gut Microbiota-Database (BGM-Db)。通过1组东方蜜峰及1组西方蜜蜂(Apis mellifera)的肠道菌群高通量测序结果, 分析不同数据库的表现, 我们发现相比于SILVA和Ribosomal Database Project (RDP), BGM-Db对蜜蜂肠道16S rRNA高通量测序短序列实现了菌种级别的分类, 分辨率更高。 相似文献
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【背景】肠道菌群在对虾的生理活动中起关键作用。日本囊对虾是我国海水养殖虾类中的主要品种之一,迄今为止有关其肠道菌群结构与功能的研究还鲜有报道。【目的】利用高通量测序技术探究日本囊对虾肠道菌群的组成结构与功能作用,揭示虾体肠道菌群与外源菌群结构间的相关性。【方法】60 d的养殖周期结束后,分别采集日本囊对虾肠道样品(归为虾肠组,n=3)、养殖水体样品(归为水体组,n=3)和对虾饲料样品(归为饲料组,n=3),提取各样品总DNA进行16SrRNA基因扩增子测序,基于生物信息学方法分析与比较样品间的菌群结构特征,并使用PICRUSt软件预测日本囊对虾肠道菌群功能。【结果】3组样品测序共获得822 713条有效序列,抽平处理后可聚类为3 416个OTU。虾肠组样品中有28.49%、59.30%的OTU可以依次在水体组、饲料组样品中检测到。门水平上,虾肠组样品中的优势菌门为变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)和梭杆菌门(Fusobacteria)。水体组、饲料组与虾肠组样品中的优势菌门结构不尽相同,但均由变形菌门和拟杆菌门组成。属水平上,虾肠组样品中的优势菌属包括弧菌属(Vibrio)、另类弧菌属(Aliivibrio)、假交替单胞菌属(Pseudoalteromonas)、假黄棕杆菌属(Pseudofulvibacter)、科尔韦尔氏菌属(Colwellia)、小纺锤状菌属(Fusibacter)、发光杆菌属(Photobacterium)、脱硫弧菌属(Desulfovibrio)、嗜冷杆菌属(Psychrobacter)以及弓形杆菌属(Arcobacter)。水体组和饲料组中检出的核心菌属结构与虾肠组相比有明显差异,其中海命菌属(Marivita)和假单胞菌属(Pseudomonas)分别为养殖水体及对虾饲料样品中的最优势菌属。PICRUSt预测结果显示,日本囊对虾肠道菌群的基因功能主要与新陈代谢类功能有关,包含氨基酸代谢、碳水化合物代谢与能量代谢等。【结论】日本囊对虾肠道菌群与其他种类对虾肠道菌群的结构间存在共性,其形成在一定程度上受到了外源菌群的干预,并在虾体的日常代谢活动中发挥了一定的作用。 相似文献
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Zhongqiu Wang Qingxin Wang Xia Wang Li Zhu Jie Chen Bailin Zhang Ye Chen Zhiyong Yuan 《Journal of cellular and molecular medicine》2019,23(5):3747-3756
Radiation enteritis (RE) is the most common complication of radiotherapy for pelvic irradiation receivers. Herein we investigated the alterations in gut microbial profiles and their association with enteritis in patients undergoing pelvic radiotherapy. Faecal samples were collected from 18 cervical cancer patients during radiotherapy. Microbiota profiles were characterized based on 16S rRNA sequencing using the Illumina HiSeq platform. Epithelial inflammatory response was evaluated using bacterial‐epithelial co‐cultures. Dysbiosis was observed among patients with RE, which was characterized by significantly reduced α‐diversity but increased β‐diversity, relative higher abundance of Proteobacteria and Gammaproteobacteria and lower abundance of Bacteroides. Coprococcus was clearly enriched prior to radiotherapy in patients who later developed RE. Metastat analysis further revealed unique grade‐related microbial features, such as more abundant Virgibacillus and Alcanivorax in patients with mild enteritis. Additionally, using bacterial‐epithelial co‐cultures, RE patient‐derived microbiota induced epithelial inflammation and barrier dysfunction, enhanced TNF‐α and IL‐1β expression compared with control microbiota. Taken together, we define the overall picture of gut microbiota in patients with RE. Our results suggest that dysbiosis of gut microbiota may contribute to development and progression of RE. Gut microbiota can offer a set of biomarkers for prediction, disease activity evaluation and treatment selection in RE. 相似文献
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Microbial compositions of human and animal feces from South Korea were analyzed and characterized. In total, 38 fecal samples (14 healthy adult humans, 6 chickens, 6 cows, 6 pigs and 6 geese) were analyzed by 454 pyrosequencing of the V2 region of the 16S rRNA gene. Four major phyla, Actinobacteria, Proteobacteria, Firmicutes and Bacteroidetes, were identified in the samples. Principal coordinate analysis suggested that microbiota from the same host species generally clustered, with the exception of those from humans, which exhibited sample-specific compositions. A network-based analysis revealed that several operational taxonomic units (OTUs), such as Lactobacillus sp., Clostridium sp. and Prevotella sp., were commonly identified in all fecal sources. Other OTUs were present only in fecal samples from a single organism. For example, Yania sp. and Bifidobacterium sp. were identified specifically in chicken and human fecal samples, respectively. These specific OTUs or their respective biological markers could be useful for identifying the sources of fecal contamination in water by microbial source tracking. 相似文献
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Saria Otani Aram Mikaelyan Tânia Nobre Lars H. Hansen N'Golo A. Koné Søren J. Sørensen Duur K. Aanen Jacobus J. Boomsma Andreas Brune Michael Poulsen 《Molecular ecology》2014,23(18):4631-4644
Gut microbes play a crucial role in decomposing lignocellulose to fuel termite societies, with protists in the lower termites and prokaryotes in the higher termites providing these services. However, a single basal subfamily of the higher termites, the Macrotermitinae, also domesticated a plant biomass‐degrading fungus (Termitomyces), and how this symbiont acquisition has affected the fungus‐growing termite gut microbiota has remained unclear. The objective of our study was to compare the intestinal bacterial communities of five genera (nine species) of fungus‐growing termites to establish whether or not an ancestral core microbiota has been maintained and characterizes extant lineages. Using 454‐pyrosequencing of the 16S rRNA gene, we show that gut communities have representatives of 26 bacterial phyla and are dominated by Firmicutes, Bacteroidetes, Spirochaetes, Proteobacteria and Synergistetes. A set of 42 genus‐level taxa was present in all termite species and accounted for 56–68% of the species‐specific reads. Gut communities of termites from the same genus were more similar than distantly related species, suggesting that phylogenetic ancestry matters, possibly in connection with specific termite genus‐level ecological niches. Finally, we show that gut communities of fungus‐growing termites are similar to cockroaches, both at the bacterial phylum level and in a comparison of the core Macrotermitinae taxa abundances with representative cockroach, lower termite and higher nonfungus‐growing termites. These results suggest that the obligate association with Termitomyces has forced the bacterial gut communities of the fungus‐growing termites towards a relatively uniform composition with higher similarity to their omnivorous relatives than to more closely related termites. 相似文献
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Jianghui Zhang Xueqing Zhang Kexin Zhang Xiaoyan Lu Guojing Yuan Huayu Yang Haiyun Guo Zhihui Zhu Tianli Wang Jiahu Hao Ying Sun Puyu Su Zhihua Zhang 《Polish journal of microbiology》2022,71(2):241
With the development of genome sequencing, many researchers have investigated the mechanism by which the intestinal microbiota influences sleep across the brain-gut axis. However, the relationship between gut microbiota and sleep disorder remains unclear. Thus, we studied the difference in gut microbiota composition between poor sleep quality- and normal populations, which helps set the ground for future research. The recruited college students provided baseline information and stool samples and completed the Pittsburgh Sleep Quality Index (PSQI). We compared the two groups’ gut microbiota composition and functional differentiation by using the 16S rRNA gene sequencing analysis. The main bacterial difference and the most critical effect were mainly concentrated within Tenericutes and Elusimicrobia. Compared with the healthy control group, some functions of the gut microbiota were impaired in the poor sleep quality group, such as butanoate metabolism and propanoate metabolism. Bacterial taxa with significant differences raised the possibility for future diagnosis and treatment of sleep problems. 相似文献
20.
T.P.R.A. Legrand M.L. Wos-Oxley J.W. Wynne L.S. Weyrich A.P.A. Oxley 《Journal of applied microbiology》2021,131(5):2528-2538