纤恙螨属恙螨一新种：蜱螨亚纲：恙螨科

从采自新疆博乐县阿拉山口(海拔290 m)室内灰仓鼠伏龙芝亚种 Cricetulus migratorius caesius耳壳内的一批纤恙螨标本鉴定中发现中国新记录亚属——爱柯纤恙螨亚属 Ericotrombidium中的一个新种,定名为博乐纤恙螨 Leptotrombidium (E.) bolei sp.nov.,它与美丽纤恙螨L.(E.)pulchrum(Sosnina,1950)及索氏纤恙螨L.(E.)sokolovi Kudryshova,1984比较近似,但本新种IP及盾板量度除 AM外均较大,PW-SB≥PL,可与前者区别;IP较小,平均787,幅度763—821,DS较少,排列规则:2+8.6.6.4.2=28,可与后者区别。     

恙螨科二新种记述(蜱螨亚纲:恙螨科)

描述恙螨科Trombiculidae 2新种：多毛恙螨属的湖东多毛恙螨Multisetosa hudongensis sp.nov.和新恙螨属的河湟新恙螨Neotrombicula hehuangensis sp.nov.,标本均存放在青海省地方病预防控制所。     

青海禽棒恙螨属一新种：蜱螨亚纲：恙螨科

本文记述从朱雀Garpodacus erythrinus(Pallas)体上采到的禽棒恙螨属Ornithogastia Vercammen-Grandjean,1960,一新种,朱雀禽棒恙螨Ornithogastia erythinae sp.nov.标本采自青海省海南藏族自冶州贵南县。     

云南恙螨五新种（蜱螨亚纲：恙螨科）

本文报道云南恙螨五新种：二毛爬虫恙螨,泸水爬虫恙螨,河口长鞭恙螨,耿马囊样恙螨,巧家囊棒恙螨。     

青海恙螨亚科三新种：蜱螨亚纲：恙螨科

本文记述了恙螨亚科Trombiculinae Ewing 1929的三个新种:1.球形钳齿恙螨,新种,Cheladonta globosea sp.nov..2.久治真棒恙螨,新种,Euschoengastia jiuzhiensis sp.nov.3.民和囊棒恙螨,新种Ascoschoengastia minheensis sp.nov.,分别采自青海省久治县、化隆县、民和县和互助县。     

翼手恙螨属二新种记述（蜱螨亚纲：恙螨科）

本记述了翼手恙螨属二新种：菊蝠翼手恙螨Chirioptella(Chiroptella)chrysantheurn，sp，nov，粗毛翼手恙螨Chirioptella(Chiroptella)chrysantheurn，sp，nov。     

同恙螨属一新种(蜱螨亚纲:恙螨科)

梨形同恙螨,新种Cotrombicula pyriformis sp.nov.采自江西省弋阳县中华鼠耳蝠Myotis chinensis的翼膜上,为我国同恙螨属螨种初记。     

纤恙螨属一新种：真螨目：恙螨科

本文报道1983年采自吉林省和龙县棕背(鼠平) Clethrionomys rufocanus 体上的一种新恙螨,棕(鼠平)纤恙螨 Leptotrombidium rufoconum sp.nov,模式标本分别存放福建医学院寄生虫学教研室和沈阳军区后勤部军事医学研究所。     

长鞭恙螨亚属在中国的发现及其一新种的描述(蜱螨亚纲:恙螨科)

长鞭恙螨亚属Lorillatum由Nadchatram(1963)建立之初,归在纤恙螨属Leptotrombidium中,后来,Vercammen-Grandjean(1968)将其改归翼手恙螨属Chiroptella,至1971年,V.-G.和Longston二氏才将其提升为属;温廷桓(1984)鉴于一直归属置疑的通什恙虫Trombicula(?Trombicula)tungshihensis Hsu and Chen,1964的某些重要特征介乎长鞭恙螨属与翼手恙螨属之间而较接近前者,为此将可考虑建立一个亚属,但当时认为仍只能置疑。赵善贤(1985)研究了较大量的通什恙虫的海南岛标本     

无前恙螨属二新种(蜱螨目:恙螨科)

本描述了采自江苏黑线姬鼠体上的无前恙螨属二新种,即长盾无前恙螨Walchia(Walchia)longiscuta Xia et Chen,sp．nov．和孟氏无前恙螨Walchia(Walchia)meni Xiaet Chen,sp．nov．。模式标本保存于复旦大学上海医学院寄生虫学教研室。     

A homozygous female hemophilia A

BACKGROUND:

Hemophilia A (HA), being an X-linked recessive disorder, females are rarely affected, although they can be carriers.

AIMS:

To study the mutation in F8 gene in an extended family with a homozygous female HA.

MATERIALS AND METHODS:

All the seven affected members (six males and one female) were initially screened by Conformation Sensitive Gel Electrophoresis (CSGE) and direct DNA sequencing.

RESULTS:

A homozygous missense mutation c.1315G>A (p.Gly420Ser) was identified in exon 9 of F8 gene in homozygous state in the affected female born of 1° consanguinous marriage and in all the affected male members of the family. Her factor VIII levels was found to be 5.5%, vWF:Ag 120%.

CONCLUSION:

In India, as consanguineous marriages are very common in certain communities (up to 30%), the likelihood of encountering female hemophilia is higher, although this is the first case of HA out of 1600 hemophilia families registered in our Comprehensive Haemophilia Care Center. Genetic diagnosis in such cases is not necessary as all the male children will be affected and daughters obligatory carriers.     

Aminopeptidase A is angiotensinase A

Summary Quantitative histochemical measurements of aminopeptidase A (APA; E.C.3.4.11.7) were done kinetically in the kidney glomeruli of rat and mouse with an instrumental setup consisting of a microdensitometer and a computer-supported morphometric system. The histochemical demonstration of APA was carried out using the simultaneous azo coupling technique (purest-grade Fast Blue B as coupling agent and -l-glutamic acid-4-methoxy-2-naphthylamide as substrate). The methodological studies show that APA activity is calcium-ion-dependent and increases linearly with the thickness of the tissue section (3–12 m) and that the time-course of APA activity as determined by linear regression is linear only for the first 1 to 2 min of the reaction. — Kinetic measurements indicate a 40% decrease in APA activities when -l-glutamic acid-4-methoxy-2-naphthylamide (-l-Glu-MNA) is replaced by -l-aspartic acid-4-methoxy-2-naphthylamide. When -l-Glu-MNA is replaced with l-alanine-4-methoxy-2-naphthylamide, which is a substrate of aminopeptidase M (APM) only very low reaction rates are measurable (about 1.4% of those with -l-Glu-MNA). 100 and 130 mM NaCl in the incubation medium increase APA activities by approximately 16%–17%. — To clarify the functional importance of APA in the kidney, their activities were measured under the influence of angiotensins. The glomerulus was selected as the measuring site, for besides APA it contains no APM or other peptidases that could degrade angiotensins (the glomerular dipeptidyl peptidase IV is not inhibited by angiotensin II). Using the Lineweaver-Burk plot, we determined a K _m of 0.16 mM for the APA in rat glomeruli and 0.14 mM in mouse glomeruli. The V _max in mouse glomeruli is 1.6 times higher than in rat glomeruli. Ang iotensin I, II and III competitively inhibit APA in the rat and mouse glomeruli. — With quantitative histochemical techniques it was possible to show that APA is equivalent to angiotensinase A (splitting off the N-terminal aspartic acid from angiotensin I and II).Supported by the Deutsche Forschungsgemeinschaft (SFB 105)     

A glucoside of urospermal A

The aerial parts of Urospermum picroides afforded, in addition to urospermal A a p-hydroxylphenyl acetate of a glucoside of urospermal A.     

Quadruplexes of human telomere DNA analogs designed to contain G:A:G:A,G:G:A:A,and A:A:A:A tetrads

Replacement of two to four guanines by adenines in the human telomere DNA repeat dG₃(TTAG₃)₃ did not hinder the formation of quadruplexes if the substitutions took place in the terminal tetrad bridged by the diagonal loop of the intramolecular antiparallel three‐tetrad scaffold, as proved by CD and PAGE in both Na⁺ and K⁺ solutions. Thermodynamic data showed that, in Na⁺ solution, the dG₃(TTAG₃)₃ quadruplex was destabilized, the least by the two G:A:G:A tetrads, the most by the G:G:A:A tetrad in which the adenosines replaced syn‐guanosines. In physiological K⁺ solution, the highest destabilization was caused by the 4A tetrad. In K⁺, only the unmodified dG₃(TTAG₃)₃ quadruplex rearranged into a K⁺‐dependent quadruplex form, none of the multiple adenine‐modified structures did so. This may imply biological consequences for nonrepaired A‐for‐G mutations. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 880–886, 2010.     

A monoclonal antibody to ochratoxin A

The production of patulin by P. expansum Link ex Gray growing in apple juice was determined over a range of pH. Patulin was found to be produced in large quantities over a narrow range of pH from 3-2 to 3–8. The biomass produced increased with increasing pH. The changes in pH due to growth of the organism were found to be small.