共查询到20条相似文献,搜索用时 968 毫秒
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Dinchuk JE Henderson NL Burn TC Huber R Ho SP Link J O'Neil KT Focht RJ Scully MS Hollis JM Hollis GF Friedman PA 《The Journal of biological chemistry》2000,275(50):39543-39554
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D L Haviland J C Haviland D T Fleischer R A Wetsel 《The Journal of biological chemistry》1991,266(18):11818-11825
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Manli Shen Stanislav Bellaousov Michael Hiller Pierre de La Grange Trevor P. Creamer Orit Malina Ruth Sperling David H. Mathews Peter Stoilov Stefan Stamm 《Nucleic acids research》2013,41(6):3819-3832
The serotonin receptor 2C plays a central role in mood and appetite control. It undergoes pre-mRNA editing as well as alternative splicing. The RNA editing suggests that the pre-mRNA forms a stable secondary structure in vivo. To identify substances that promote alternative exons inclusion, we set up a high-throughput screen and identified pyrvinium pamoate as a drug-promoting exon inclusion without editing. Circular dichroism spectroscopy indicates that pyrvinium pamoate binds directly to the pre-mRNA and changes its structure. SHAPE (selective 2′-hydroxyl acylation analysed by primer extension) assays show that part of the regulated 5′-splice site forms intramolecular base pairs that are removed by this structural change, which likely allows splice site recognition and exon inclusion. Genome-wide analyses show that pyrvinium pamoate regulates >300 alternative exons that form secondary structures enriched in A–U base pairs. Our data demonstrate that alternative splicing of structured pre-mRNAs can be regulated by small molecules that directly bind to the RNA, which is reminiscent to an RNA riboswitch. 相似文献
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L Chiariotti A L Brown R Frunzio D R Clemmons M M Rechler C B Bruni 《Molecular endocrinology (Baltimore, Md.)》1988,2(11):1115-1126
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Y Furutani M Notake T Fukui M Ohue H Nomura M Yamada S Nakamura 《Nucleic acids research》1986,14(8):3167-3179